판막 질환에서의 심근 섬유화 : 심근 조직을 이용한 형태학적 연구 및 혈청 생화학적 지표의 분석

강수진,박덕우,송재관,박경민,송종민,강덕현,한기훈,송현,이재원,주석중,송명근,김정선 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.2

지속성 외래 복막투석 환자에서 염증 지표와 고해상도 B 모드 경동맥 초음파 소견의 연관성에 관한 연구

송현용,송영수,안철우,강신욱,최규헌,하성규,이현철,이호영,한대석 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.2

배 경 : 저알부민혈증 및 C-반응 단백(C-reactive protein, CRP)이 증가된 지속성 외래 복막투석 환자의 사망률이 높은 정확한 이유는 밝혀져 않다. 혈액투석 환자에서처럼 복막투석 환자에서 저알부민혈증 및 염증반응이 심혈관계 질환 위험인자 인지 알아보고자 하였다. 방 법: 비당뇨병성 말기 신부전, 지속성 외래 복막투석 환자 93명의 심혈관계 질환 위험인자들과 복막 투과 특성 및 알부민, CRP 등의 염증지표에 따라 고해상도 초음파를 이용한 경동맥내막-중간막 두께, 내막-중간막 면적, 플라크의 유무 등을 단면적으로 비교하였다. 결 과: 복막 투과 특성은 고투과막군 14명, 중간 고투과막군 38명, 중간 저투과막군 31명, 저투과막군 10명으로 각 군간의 경동맥 내막-중간막 두께, 플라크 유무 및 내막-중간막 면적의 차이는 없었다. 기존 관상동맥 질환 환자는 8예(8.6%)로 내막-중간막 두께는 관상동맥 질환이 없는 환자보다 증가되었고(0.79±0.21 vs 0.60±0.11 mm, p<0.05) 플라크 형성은 더 많이 보이나 통계적 유의성은 없었다(75.0 vs 63.5%, p>0.05). 혈청 알부민치와 경동맥 내막-중간막 면적은 역상관관계를 보였다(r=-0.27, p<0.05). 저알부민혈증 환자(혈청 알부민 <3.5 g/dL)는 정상 알부민혈증 환자보다 내막-중간막 두께가 증가되었고(0.67±0.15 vs 0.61±0.12 mm, p<0.05), 플라크도 유의하게 많았다(68.0 vs 55.8%, p<0.05). 혈청 CRP치와 경동맥 내막-중간막 면적은 양의 상관관계가 있었으며(r=0.21, p<0.05) CRP가 높은 환자(≥0.8 mg/dL, n=18)에서플라크가 많이 관찰되었으나(65.8 vs 50.0%, p<0.05) 경동맥 내막-중간막 두께 및 내막-중간막면적은 양군간에 차이가 없었다. 다변량 회귀 분석상 경동맥 내막-중간막 두께에 영향을 미치는 인자는 고령, 높은 CRP, 기존 심혈관계질환, 낮은 혈청 알부민 순이였다. 결 론 : 복막투석 환자에서 고해상도 초음파를 이용한 경동맥 내막-중간막 두께 측정은 심혈관계 질환 유무 예측의 비침습적 검사가 될 수 있고 저알부민혈증 및 높은 CRP과 같은 만성염증 지속상태가 복막투석 환자에서 죽상 동맥 경화증의 발생을 증가시킨다고 가정할 수 있다. Background : Continuous ambulatory peritoneal dialysis(CAPD) patients with low albumin(LA) and signs of inflammation reflected by increased C-reactive protein(CRP) level have an increased mortality, but the mechanism of this phenomenon is not clear yet. Methods : To answer whether LA and inflammation also enhance cardiovascular risk in CAPD patients, we performed cross sectional study measuring carotid artery intima-media thickness(IMT), calculated intima-media area(cIM area) and the presence of plaque by high-resolution B-mode ultrasonography in 93 non-diabetic CAPD patients. Results : Patients with coronary artery disease (CAD, n=8) had significantly increased IMT(0.79± 0.21 mm vs. 0.60±0.11 mm, p<0.05) and higher prevalence of carotid plaques(75.0% vs. 63.5%) compared to the non-CAD patients. Significant inverse correlation was observed between serum albumin (SA) level and cIM area(r=-0.27, p<0.05). Those patients with LA(SA <3.5 g/dL) had significantly increased IMT compared to non-LA patients(0.67±0.15 mm vs 0.61±0.12 mm, p<0.05). Prevalence of carotid plaques was also significantly higher in LA patients (68.0% vs. 55.8%, p<0.05). CRP level revealed a significant positive correlation with cIM area(r=0.21, p< 0.05). Patients with high CRP(≥0.8 mg/dL, n=18) had higher prevalence of carotid plaques (65.8% vs. 50.0%, p<0.05) compared to those patients with CRP <0.8 mg/dL, but IMT and cIMT area were not different. By multivariate logistic regression analysis, old age, high CRP, history of CAD and low SA were the independent risk factors affecting IMT. Conclusion : Our study strongly suggests that low albumin and chronic inflammatory state of CAPD patients could be associated with increasing atherosclerotic cardiovascular disease. (Korean J Nephrol 2002;21(2):285-294)

태양열 온수급탕 시스템에 관한 연구 : 아파트를 중심으로 With relevance to Apartment

송인춘,김기세,구현덕,김병수,나기태 忠南大學校 産業技術硏究所 1999 산업기술연구논문집 Vol.14 No.1

This work was carried out to propose a model of the most effective layout and installation for applying the Solar Hot Water system to apartment and to offer the basic data for promoting the destribution of this Solar Hot Water system in the country. For this purpose, the performed experiments are as followings: 1)Measurement and analysis of the amount of hot water adn the cost of water used in apartment of which the area is 30 to 40 pyung. 2)to esimate the efficiency of Solar Hot Water System in accordance with an angle of inclination. 3) Analysis of economical efficiency the annual expected saving cost produced by replacing the existing hot water system with the Solar Hot Water System. 4)Proposal of the best model for installing the Solar Hot Water System in apartment by considering the place and sunshine,and compaison of merit and demerit of this system.

정상 성인의 말초 혈액 림프구에 표현된 Humkv325-derived κ 경쇄에서의 N-region addition

송준현,이찬희,이지수,이수곤 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.2

Complementarity determining region(CDR3) of immunoglobulin is the center of antigen binding site. Changes in the number of amino acids of CDR3 may result in change in antigen binding specificity and affinity. The majority of immunoglobulin x chain has 9 or 10 amino acid co-dons in the CDR3 region. Immunoglobulin x chain containing CDR3 with 11 amino acid codons cannot be made without N-region addition. The aims of this study were to evaluate the presence of immunoglobulin x light chain with 11 amino acid codons in its CDR3 domain expressed in the peripheral blood lymphocytes(PBLs) of normal individuals and to correlate amino acid codon length in CDR3 region with age and sex. RNA was isolated from PBLs of normal individuals and x chain was PCR amplified. Amino acid length of CDR3 region was determined by polyacrylamide gel electrophoresis. A low but constant expression of immunoglobulin x chain with 11 amino acid codons in CDR3 region in normal individuals was found (mean±SD=11.72±1.80%). Significant correlation was not found between amino acid length in CDR3 region of x chain and age or sex. These findings suggest that the concept that N-region addition in x light chain does not occur due to absence of terminal deoxynucleotidyl transferase (TdT) needs to be reconsidered. The existence of long CDR3 K chain in PBLs of normal individuals may reflect that CDR diversity in is quite common. Amino acid length of x chain CDR3 is not regulated during development and there is no influence of sex on the expression of x chain containing CDR3 with 11 amino acids.

有限要素法을 이용한 過壓密粘土의 壓密沈下豫測

현상락,류제천,김송만,박춘수 충남대학교 산업기술연구소 2002 산업기술연구논문집 Vol.17 No.1

Consolidation is the time-dependent settlement of soils resulting from the expulsion of water from the soil pores. When a saturated compressible clay layer is subjected to a stress increase, consolidation settlement occurs greatly. Because the hydraulic conductivity of clay is significantly small, the excess pore water pressure generated by loading gradually dissipates over a long period. The settlement caused by consolidation in clay may be several times greater than the immediate settlement. A number of researches are carried out to predict the consolidation behavior in field, however, most of researches show the discrepancies between the prediction and the field settlement data. Therefore, the estimation of consolidation rate is one of the important factors in the construcion on soft clayey deposits. In this paper, laboratory tests have analyzed to apply accurately the soil parameters that executed the plan of stage construction. This study analyzes consolidation behavior of overconsolidated clay with two-dimensional consolidation analysis program by use of the numerical method. By using this program, the time dependent consolidation behavior of soft deposits can be analyzed appropriately. Elastic and elastic-plastic finite element analysis are compared with Terzaghi's theory of one-dimensional consolidation.

뇌동맥류의 진단에 대한 전산화단층촬영 뇌혈관조영술과 고식적 뇌혈관조영술의 비교

송상현,윤수한,안영환,안영민,조기홍,조경기,김선용,서정호 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.10

Cerebral angiography has been essential for the diagnosis of the intracranial aneurysms but. is sometimes accompanied by serious complications. Resolution of CT angiography was up-graded greatly to represent the three-demensional structure of vesseles since helical CT had been introduced. We have compared 26 cases of CT angiography and 28 cases of conventional angiography in terms of specificity and sensitivity for the diagnosis detectable aneurysm diameter. configurational diagnosis and diagnostic confidency. All results showed no statistical difference between CT angiography and conventional angiography. These should suggest that CT angiography could be replaced with conventional angiography for the diagnosis of ruptured aneurysms and even of unruptured aneurysms. resulting in the introduction of first screening modality of unruptured aneurysms.

NiAl/Ni₃Al 2상 합금의 상변태 및 미세조직에 미치는 제3원소 첨가효과

송성훈,김수현,오명훈,위당문 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.12

The microstructural evolution of b-NiAl/γ' -Ni3Al two-phase (Ni_(66)Al_(34))_(100-x)X_(X)(X=Ti, Si, Nb) alloys during various heat treatment (quenching and aging) were systematically studied. It was found that the microstructural features of the two-phase NiAl/Ni₃Al alloys could be divided into three types : lamellar, mesh and Widmansttten microstructures. The reason why those three types of microstructure were formed could be explained by the change of austenite start temperature(As temperature) depending on the ternary elements. In case of As>250℃, the lamellar microstructure was formed by following phase transformation: Martensite→Ni5Al3→β+γ' . In this case, the NiAl martensite was quickly transformed into the Ni5Al3 phase at 250℃ by a re-ordering reaction. In case of 20℃ <A_(s)<250℃, two types of mesh microstructure were formed depending on the ternary element. When Ti and Nb were added as a ternary element, the β→Ni_(5)Al3 transformation occurred very quickly, but not in the case of Si addition. Hence, the final mesh microstructures showed somewhat different microstructural features. On the other hand, in case of A_(s)<20℃, the direct β→β+γ' transformation occurred because the martensitic transformation could not occur due to its very low M_(s) temperature, resulting in the Widmanstatten type microstructure.

Baculovirus를 이용한 Aujeszky's Disease Virus gⅢ 단백질 발현

송재영,이중복,현방훈,박종현,김병한,권창희,전무형,안수환 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-

The g Ⅲ gene located in U_L region of Yangsan strain, a field isolate of Aujeszky's disease virus (ADV) in Korea, was cloned into pTZ18R and sequenced. The gⅢ gene consisting of 1,437 nucleotides showed 98% sequence homology with that of Becker strain, a reference strain of ADV. The gene encoding gⅢ of Yangsan strain was placed under the control of Autographa californica nucleopolyhedrosis virus (AcNPV) polyhedrin promoter, and expressed by the derived recombinant baculovirus using Spodoptera frugiperda 9 (Sf9) cells. The expressed gⅢ was a protein with molecular weight of 72kd determined by immunoprecipitation and Western blotting assay using anti-ADV polyclonal antibodies and anti-gⅢ monoclonal antibody. The partially purified gⅢ protein was utilized as antigen in the radial immunodiffusion enzyme assay (RIDEA) to detect to specific antibody against ADV in pig sera. The results indicated that the sensitivity of RIDEA with the recombinant gⅢ protein antigen (98%) was as high as that with the conventional glycoprotein antigen extracted from the ADV infected cells. In addition, the false positive and false nagative reactions in gⅢ RIDEA were significantly reduced than the conventional glycoprotein RIDEA as judged from the results of standard serum neutralization test.

백서의 복강내에 투여한 Mitomycin-C-Polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

송규상,강대영,최정목,노승무,정경수,오정연,김진향,양준목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.

저염농도의 자리발효식품의 가공에 관한 연구

송대진,김재하,강영주,김수현,고영환,하진환 濟州大學校 工科大學 産業技術硏究所 1990 産業技術硏究報告 Vol.1 No.-

This study was carried out to obtain the basic data on salted and fermented damsel fish(Jari-Jeot), one of the important traditional marine foods in Cheju-Do. and also to seek salt lowering method for the development of a new local tourist food. The results are as follows : The best organoleptic results were obtained after 60 days' fermentation for 25.0, 12.5 and 10% of salt-added group, and around 45 days for 7.5 and 5% of salt-added group. In raw fish ingredient IMP was abundant which marked 17.7 μmole/g while in fermented fish hypoxanthine was predominant but ATP and ADP were not detected after 30 days' fermentation. Sixteen kinds of free amino acids were detected and identified in Jari-Jeot instead of 17 kinds of those in raw sample. The abundant amino acids in Jari-Jeot after 60 days' fermentation were lysine. alanine, glutamic acid, glycine and leucine, and those were consisted of approximately 60% of the total free amino acids. During fermentation the content of TMAO was decreased gradually and no TMAO was detected after 75 days' fermentation while that of TMA was increased during fermentation up to 45 days' and decreased afterwards. The fatty acid of low-salt Jari-Jeot was composed of 31.9% of saturated acids, 13.2% of monoenoic acids and 54. 9% of of polyenoic acids. While the content of EPA in polyenoic acids was 1.9%, that of DHA was 23.0%. In order to lower salt concentaration of fermented damsel fish, additon of Pediococcus halophilus culture as a starter together with 10-12.596 of salt, 4-5% of KCI, and 2% of glucose gave as good quality as traditionally made Jari-Jeot.