백서 척수후근절로부터 슈반세포의 효과적인 체외 배양법

김성민,이종호,김남열,안강민,최원재,최시호,차미주,이주영,황순정,장정원,명훈,최진영,서병무,정필훈,김명진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.4

Schwann cells(SCs), an important component of the peripheral nervous system, intract with nerous to mutually support growth and replication for the peripheral nerve regentation. Recently, ading SCs to the lumen of guidance channel is widely tried to improve regeneration or to make regeneration possible over otherwise irreparable gaps. however, it is not easy to isolate and multiplicate SCs as much as enough to help the axonal regeneration. For the allogeneic SCs source for tubular nerve guidance, we developed a little bit improved technique of harvesting and multiplicating SCs. by culturing dispersed dorsal root ganglia in specially designed medium with growth factors and serial processing, we repeatedlly generate relatively homogenous SC cultures. Our technique was compared with other methods of literature using immunostaining methods such as GFAP, S100, BDNF and the total SC count assessment at different time interval after primary culture.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

버들치(Rhynchocypris oxycephalus)의 vitellogenin 생성량 측정을 이용한 수중 estrogen휴 화합물의 정량분석법에 관한 연구

김을환,이도부,한상훈,윤병수 경기대학교 부설 기초과학연구소 2005 경기대학교 기초과학논문집 Vol.18 No.1

Vitellogenin(Vtg), a phospholipoglycoprotein precursor of egg yolk, is synthesized and secreted from the liver in response to estrogens in female fish. Vtg is normally undetectable in the blood of male fish, but can be induced by exposure to chemicals possessing estrogenic activity. Thus, the presence of Vtg in blood of male fish can serve as a useful biomarker for assessing previous exposure to estrogenic compounds. In the present study, Vtg was abnormally expressed in Rhynchocypris oxycephalus treatment of Estradiol Benzoate (E_(2)). As the result, it was found that the level of Vtg in blood from R. oxycephalus was increased by treated quantity of E_(2) with dose-effect manner.

재원일수 단축에 따른 병상당 연간 의료수익 증대효과 분석

김영훈,윤병준 서울보건대학 1997 서울보건대학 부설 병원경영연구소 논문집 Vol.3 No.1

As medical insurance system and medical delivery system has been expanded, the demand of medical service at the tertiary medical institutions has been increasing rapidly. There has been shortage of beds in these hospitals and effective management of beds had to be considered. For the efficient utilization of the exsisting hospital beds, bed turnover rate has to be high and their length of stay in hospital has to be shortened. The sample of this study was in-patients admitted in 10 clinical departments of a tertiary hospital in Seoul. Daily medical fees through length of stay in hospital were observed and we analyzed the increase of hospital revenues per bed for the shortening of length of stay. The results of the analysis were as follows : 1. The trend of daily medical fees of in-patients admitted in dept. of digestive internal medicine, respirative internal medicine, circulatory internal medicine, general surgery, nervous surgery, orthopedic surgery, gynecology, pediatrics, urology, E.N.T. was the highest from the first day to the third day. Because most necessary examination and various treatment or operation took place in these period. 2. The estimative model for medical fees by the length of stay at each clinical department was inferred. 3. The increased revenue per bed by shortening the length of stay was calculated by the estimative model. Shortening one day would increase 16,168 thousand won in dept. of gynecology, 12.926 thousand won in dept. of E.N.T., 1,938 thousand won in dept. of digestive internal medicine, 4,227 thousand won in dept. of circulatory internal medicine. 739 thousand won in dept. of respiratory intrnal medicine, 2,375 thousand won in dept. of general surgery,1,752 thousand won in dept of nervous surgery, 2,236 thousand won in dept. of orthopedic surgery, 655 thousand won in dept. of pediatrics, 5.664 thousand won in dept. of urology. It is expected that they can improve their profitability by shortening the length of stay of the in-patients.

마케팅 전략수립을 위한 병원이미지 평가

김영훈,윤병준 서울保健大學 1994 論文集 Vol.14 No.1

This study was carried out to provide the basic data for a distinctive planning to improve hospital image as a part of marketing strategy. The purpose of this study was to investigate the factors that have either affirmative of negative effect on hospital image and selection. For this study, questionaires were sent to the 1000 persons in Sungnam city. Because it is predicted that the competition among the hospitals in Sungnam will be high in securing patients. Only 377 cases that had utilized three general hospitals(H hospital, J hospital, M hospital) were available for our study. We made use of frequency analysis for general characteristics of the object cases, one-way ANOVA for distinctions among hospitals, multiple regression and path analysis for the factors and process that had influenced on hospital image and selection. The results of this study are as follows. ① When they select the hospital, the main factors to be considered were doctors' ability, kindness and sincerity, and experience and explanation. We found that services of medical personnel were more important than we expected. ② There was difference of experienced image among three hospitals. Clean and sanitary environment of hospitals, traffic convenience, short periods of waiting time, kindness and sincerity of doctors, ability and technology of doctors, modernity of medical facilities and equipments, medical cost, external reputation of hospitals and services for community were statistically significant(p<0.001, p<0.01, p<0.05). Above variables were classified according to external environment factors, internal environment factors, non-doctor service factors, doctor service factors, traffic factors. The result of one-way ANOVA analysis showed the difference in experienced image among hospitals. Non-doctor service factors were statistically significant(p<0.001). ③ As a result of path analysis, the educational level had a direct effect and reverse effect on the noncausality the total effect of educational level being 0.07317. The sex had only a direct effect in the causality but reverse effect in the noncausality, the total effect of sex being 0.06887. The marriage status had only a direct effect, the total effect of marriage status being 0.08481. The convenience factors involved cleanness and sanitation, traffic convenience, modernity of medical equipments and reputation of hospital. They had a influence on the choice and the images of hospitals(α=0.049272). The inconvenience factors involved parking lot, waiting time, kindness of doctors, admission atmosphere, medical cost and service for community, so they had a reverse effect on the choice of hospital and the hospital image.

중소기업 공동브랜드 활성화를 위한 패키지디자인 연구

김남훈,김병욱 한국패키지디자인학회 2003 상품문화디자인학연구 Vol.14 No.-

Like above, in the meantime 'Development of Joint Brand by the Small-Medium Companies' has been researched briskly as a method of enhancing the value-added of products among the Small-Medium Companies with poor competitiveness in various sides. The object of 'Development of Joint Brand' of these products is to cut the huge expenses of development for investment at the early stage when newly developing their own Brands among a lot of Small-Medium Companies at the minimum, and minimize the danger of failure in the consumption market as soon as possible, and increase the sales among the Small-Medium Companies. For the development of these Joint Brands the various Small-Medium Companies should develop the unified Brand of products with the co-operative joint, manage the quality of products and Package design as well as marketing and publicity as a whole. After all, they are effective ways to push efforts to address the Brand image at the maximum and enhance an international image. It may be low-cost and high-efficiency strategy. The method of development of Joint Brands by the domestic Small-Medium Companies in the past appears again recently. Accordingly, it starts to appear a trademark by Joint Brand strategy with new concept, not the existing method. For example, Haebito Co, Ltd., a Brand related to foods, planned a Brand first, and collected affiliats. And then they took a method to sell the products with a unified Brand after receiving the products from the 90 food companies. This is, they stepped forward from the strategy of the existing general Joint Brand, made already planned Brand with a concept of unified Brand in the fields of marketing, publicity, and distribution, etc. As a result of that, they come to obtain cost reduction effect in various aspect as well as 'Brand Power' largely. This study, 'A Study on Package design for Activating Joint Brand for Small-Medium Companies', is to use a case of Joint Brand strategy of general concept that the domestic Small-Medium Company tried to do. And it is to develop the Joint Brand by the characteristics of products with the recent unified concept. Also, the object of this study is to approach the Package design for creating new high value-added.

세계화의 위기와 대응전략에 관한 고찰

金昞勳 명지대학교 리서치아카데미 2001 리서치 아카데미 논총 Vol.4 No.-

수치해석에 의한 미소 스테인리스강 파이프의 Nd:YAG 레이저빔 절단시 Striation 생성과 절단폭에 관한 연구

金秉喆,金道熏 대한금속재료학회 2001 대한금속·재료학회지 Vol.39 No.12

The formation of a striation pattern and the variation of kerf width in a thin stainless steel tube were investigated by numerical simulation during a pulsed Nd:YAG laser cutting process. The simulated results were compared with the experimental results, which were performed under the same conditions for simulation. The simulated results showed good agreement with the experimental results. Although the formation of the striation pattern was influenced by various laser parameters, the laser power density had come out the most important factor in the formation of striation patterns. Since a high power density resulted in clear regular striation patterns, relatively low energy density caused the formation of a hot spot, which hindered the formation of regular striation patterns and caused less striation. The kerf width is increased with decreasing the power density and the hot spot is generated in the bottom of tube section in the case of low energy density condition. The numerical simulation calculations can be used to predict the shape of striation patterns and to offer a way to provide a smooth cut wall.

알코올중독자의 백혈구탐식능, 림프구아형 및 증식능

김영훈,이정녀,서병배,김용호 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1996 Journal of biomedical laboratory sciences Vol.2 No.2

알코올 중독자의 폐렴관리와 같은 건강관리에 필요한 비특이 면역력, 면역기능을 측정하기 위하여 알코올 중독 의존형으로 입원한 신, 구환자군에 대하여 다음과 같은 결과를 얻었다. 대조군에 대한 알코올 중독증은 MCV, 혈당, ΥGTP를 이용한 생물학적 측정 결과에 의하여 확인 하였으며, 기타 측정 항목은 대조군과 유사한 결과를 나타내었다. 림프구 아형 분석 결과 대조군은 한국인 참고치와 매우 유사하였으며, 실험군 CD4+를 제외한 CD3+, CD8+, CD19+이 감소되었고, CD4+/CD8+ 비율은 통계적으로 유의하게 증가되어 있음을 알 수 있었다. 따라서 실험군의 면역기능과 림프구 증식능은 대조군에 비하여 현저하게 저하되었으며, 탐식세포 탐식능 및 유주능도 대조군에 비하여 현저하게 감소되었다. 탐식세포의 유주능과 CD4+의 증가, CD4+/CD8+ 비율의 증가와 PHA에 의한 림프구 증식능의 감소 및 CD3+, CD19+ 감소등은 상호관련성을 가지고 일치된 결과를 보여 대조군에 비하여 현저하게 저하된 탐식세포의 탐식기능과 함께 알코올 중독자의 비특이 면역계, 면역기능이 현저하게 저하되어 있음을 알 수 있었다. 그러나 알코올 중독 의존형으로 입원한 신, 구환자간의 비교분석 결과 총단밸질, 혈색소, 림프구 증식능, 탐식세포 탐식능, 유주능에서 신환자군에 비하여 구환자군이 대조군에 다소 근접되어가는 결과로부터 치료에 따라 숙주의 세포성, 체액성 면역기능이 회복되어가고 있음을 알 수 있었다. 또한 결과 알코올중독자의 비특이적 면역능의 측정을 위한 탐식세포 탐식능, 유주능은 숙주, 미생물측의 활성화 자극인자가 모두 포함된 phagocytic plaque film법에 의하여 측정함이 의의 있는 평가를 할 수 있을 것으로 보며, CD4+/CD8+ 림프구 아형 비율을 이용한 면역기능의 측정은 알코올 중독자의 건강 관리를 위한 면역력 측정에 유용한 방법이라고 생각된다. Alcoholics increased susceptibility to microbial infection that is associated with decreased immunity. but there has been little experimental evidence to support alcoholics-induced increase of microbial infection directly in non-specific immunity. Therefore, we were used the method of phagocytic-plaque including all the stimulating factors for the phagocytosis, subtypes of lymphocytes and T-lymphocyte proliferation. The experimental groups were divided into 3 groups: (1) alcoholics who were hospitalized less than 1 week (newly hospitalized alcoholics), (2) alcoholics who were hospitalized more than 2 weeks (old hospitalized alcoholics), (3) healthy blood donors. We have studied 98 alcoholics and 35 healthy blood donors and control groups. A physician has checked the biological markers and diagnosed the body-condition alcoholics. The immunity and non-specific immunity on the alcoholics were analyzed by using the simultest kit and flow cytometry. Proliferation of the lymphocytes was analyzed by the phytohemmagglutinine mitogen. Phagocytosis and migration properties of leukocytes were identified on the layer formed by Staphylococcus aureus Cowan I strain. Biological markers of alcoholics and control groups, by such as blood glucose, Υ-glutamyl transpeptidase and mean corpuscular volumes of red blood cells, were determined by biochemical and hematological methods. Compared with control groups, cluster of differentiation (CD)3+, CD8+ and CD19+ in alcoholic were more decreased except CD4+/CD8+ ratio. Proliferation of the T-lymphocytes, phagocytosis and migration properties of the leukocytes in alcoholics were decreased compared with those of control groups. According to the results observed in our experiment, they can be summerized as follows: 1. Cellular, humoral and non-specific immunities, are markedly decreased in alcoholics than those in control groups. 2. It is inferred that Phagocytic plaque formation is a very useful method to evaluate phagocytosis and migration properties of the alcoholic leukocytes 3. It is thought that the subtypes of lymphocytes, especially CD4+/CD8+ ratio, are essential methods to analyzed the alcoholic immunity. 4. Specific and non-specific immunity on the old hospitalized alcoholics was slightly increased, which depends upon the alcoholic medication.

地方政府 서비스供給의 效率性에 대한 民間化의 效果

金昞勳 명지대학교 사회과학연구소 1994 사회과학논총 Vol.9 No.-