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        • 다환방향족 탄화수소에 오염된 토양에서 분리한 Pseudomonas sp. KK1의 생리학적 특성

          윤병준,강형일,오덕철 제주대학교 생명과학연구소 2001 제주생명과학연구 Vol.4 No.-

          Strain KK1 was isolated from soil contaminated with polycyclic aromatic hydrocarbons and able to mineralize anthracene, naphthalene, and phenanthrene. In this study, strain KK1 was tested to evaluate carbazole-degrading potential using radiorespirometry. When KK1 was pre-grown on phenanthrene the cells were able to mineralize carbazole much more rapidly, suggesting a possible close linkage between the pathways for catabolism of carbazole and phenanthrene. Such an increase in carbazole degradation was not appreciable when KK1 was pre-grown on naphthalene. Strain KK1 was identified as the genus Pseudomonas with over 90 % confidence based on BIOLOG system and FAME analysis. Analysis of PLFAs extracted from kk1 cells on carbazole medium revealed that lipids 10:0 3OH, 17:0 cyclo, and 18:0 were representatives produced or significantly increased in response to carbazole. Tests for antibiotics provided the data that strain KK1 was resistant to antibiotic ampicillin, but susceptibile to chloramphenicol, gentamycin, kanamycin, streptomycin, and tetracycline. Strain KK1 demonstrated strong resistance to most heavy metals such as Ba, Cd, Fe, Hg, Pb used in this study.

        • KCI등재

          세균군집의 구조분석을 통한 장기간 농약사용이 토양생태계에 미치는 영향 평가

          윤병준,김성현,이동헌,오계헌,강형일,Yun, Byeong-Jun,Kim, Seong-Hyeon,Lee, Dong-Heon,O, Gye-Heon,Gang, Hyeong-Il 한국미생물학회 2003 미생물학회지 Vol.39 No.4

          본 연구는 장기간 동안 지속적인 농약의 사용이 토양생태계에 미치는 영향을 세균군집의 구조분석을 통하여 그 관련성을 평가하고, 이에 대한 기초 자료를 얻고자 수행하였다. 30년 이상 매년 수시로 농약을 사용하여온 제주지역의 한 감귤원 토양과 비농지 토양에 존재하는 세균군집을 16S rRNA clonal library로부터 얻은 각 100 개 클론의 유전자 염기서열을 기초로 하여 비교 분석한 결과 지속적으로 농약을 사용해온 감귤원 토양에서는 3개의 아문(Proteobacteria $\alpha$, $\gamma$, $\delta$)을 포함하는 Proteobacteria를 비롯한 5개 문 18개의 속 그룹에 포함되는 세균군집의 구조를 보였고, 농약을 사용하지 않은 비농지 토양에서는 4개의 아문(Proteobacteria $\alpha$, $\beta$, $\gamma$, $\delta$)을 포함하는 Proteobacteria를 비롯한 12개 문, 44개의 속 그룹에 포함되는 군집구조를 나타냈다. 감귤원 토양에서 가장 많은 분포를 보인 세균은 Proteobacteria g group에 속하는 것으로 전체 clone의 56%로 상당한 우점현상을 나타내었고, Acidobacteria group에 속하는 균이 25%, Firmicutes group, 5%, Planctomycetes group, 2%, Proteobacteria $\alpha$와 $\delta$ group이 각 1%, Cyanobacteria group, 1% 등의 순서로 우점현상을 보였다. 반면, 비농지 토양에서는 Acidobacteria group에 속하는 균이 14%, Planctomycetes group, 13%, Proteobacteria $\alpha$, $\gamma$, $\delta$ group이 각각 10%, 9%, 9%, Firmicutes group, 8%, Verrucomicrobia group, 8%, Actinobacteria group, 6%, Proteobacteria $\gamma$ group, 3%, Bacteroidetes group, 3%, Gemmatimonadetes group, 3%, Cyanobacteria group이 1% 등의 순서로 장기간 농약을 사용해 온 토양에 비하여 훨씬 다양한 미생물군집의 분포빈도를 나타냈다. 이러한 결과는 지속적인 비료 및 농약의 사용이 토양생태계를 구성하는 세균군집의 다양성을 크게 감소시키거나 또는 특정 미생물을 소멸 시킬 수 있음을 제시해 주었다. In this study bacterial community was analyzed to evaluate the impacts of long-term use of agricultural chemicals on a soil ecosystem as well as to obtain fundamental data on the relationship. Sequences of 16S rRNA clones from a non-agricultural site and a tangerine orchard soil which has a history of long-term use of agricultural chemicals over 30 years were analyzed. This revealed that bacterial community containing 5 divisions and 18 genera was distributed in a tangerine orchard soil, while bacterial community containing 9 divisions and 44 genera was distributed. In a tangerine orchard soil site, the most abundant bacteria in subdivision level were placed into Proteobacteria γ group which occupied 56% of total clones. The other bacterial clones from the ocrhcard soil exposed to agricultural chemicals over 30 years were Acidobacteria group (25%), Fimicutes group (5%), Planctomycetes group (2%), Proteobacteria α (1%), δ group (1%), and Cyanobacteria group (1%). Whereas, the clones were from the non-agricultural site were distributed among the division or subdivision Acidobacteria group (14%), Planctomycetes group (13%), Proteobacteria α (10%), β (9%), δ (9%), Fimicutes group (8%), Verrucomicrobia group (8%), Actinobacteria group (6%), Proteobacteria γ group (3%), Bacteroidetes group (3%), Gemmatimonadetes group (3%), and Cyanobacteria group (1%). This finding suggests the possibility that long-term application of agricultural chemicals or fertilizers on a tangerine orchard might result in drastic reduction or alteration in the composition of the bacterial community in the contaminated soil site.

        • KCI등재

          중・고등학생들의 인구사회경제적 특성, 건강관련행위 및 주관적 건강인지가 학업성취도에 미치는 영향

          윤병준 한국학교·지역보건교육학회 2019 한국학교지역보건교육학회지 Vol.20 No.3

          Objectives: The purpose of this study was to examine whether demographic and socio-economic characteristics, health behaviors and health status are related to academic achievement in Korean adolescents. Methods: Data from the 14th Korea Youth Risk Behaviors web-based Survey(KYRBWS) administered in 2018 were analyzed; 60,040 students in middle and high school participated in the survey. It was conducted frequency analysis, Rao-Scott chi-square test and multiple logistic regression analysis after adjusted for variables such as age, school grade. Results: The significant influencing factors on academic achievement were family economic status, living with parents, subjective health status, smoking experience, alcohol use experience, eating breakfast, sleeping, internet use during weekdays, depressive symptom(p<.001). The odds ratio for achieving higher academic achievement compared with those who never smoked was 1.50(p<.001). Conclusion: I found that students’ undesirable health behaviors affect their academic performance. To improve academic achievement, I recommend intervention programmes such as school health promotion that reduce undesirable health related behaviors. Objectives: The purpose of this study was to examine whether demographic and socio-economic characteristics, health behaviors and health status are related to academic achievement in Korean adolescents. Methods: Data from the 14th Korea Youth Risk Behaviors web-based Survey(KYRBWS) administered in 2018 were analyzed; 60,040 students in middle and high school participated in the survey. It was conducted frequency analysis, Rao-Scott chi-square test and multiple logistic regression analysis after adjusted for variables such as age, school grade. Results: The significant influencing factors on academic achievement were family economic status, living with parents, subjective health status, smoking experience, alcohol use experience, eating breakfast, sleeping, internet use during weekdays, depressive symptom(p<.001). The odds ratio for achieving higher academic achievement compared with those who never smoked was 1.50(p<.001). Conclusion: I found that students’ undesirable health behaviors affect their academic performance. To improve academic achievement, I recommend intervention programmes such as school health promotion that reduce undesirable health related behaviors.

        • 주관적 건강상태에 영향을 미치는 관련요인 분석

          윤병준 서울保健大學 1998 論文集 Vol.18 No.1

          This study was carried out to analyze the relationship self-evaluated health status, usual health status evaluated by a member of household with social-demographic levels and health related behaviors. I obtained the health consciousness and behavior survey data which Korea Institute for Health and Social Affairs conducted national-wide interview in 1995. The sample consisted of 5,330 persons aged 20~69. I select following independent variables residence, sex, age, educational level, marital status, standard of living among social-demographic variables and smoking, drinking, physical exercise, adequate sleeping, regular meals among health behavior variables and I select self-evaluated health status, usual health status as the dependent variables. The results were as follow 1. Self-evaluated health status was significantly associated with residence, sex, age, educational level, marital status, standard of living respectively(p<0.001). 2. Self-evaluated health status was significantly associated with smoking, drinking, physical exercise, regular meals, adequate sleeping respectively(p<0.001). 3. Self-evaluated health status was highly correlated with usual health status evaluated by a member of household (r=0.6916). 4. The result of multivariate analysis of variance was that physical exercise and adequate sleeping had an effect on self-evaluated health status significantly(p<0.001). 5. The health behaviors explained 0.5 percent of self-evaluated health status variance, but covariate variables of age, educational level, sex, standard of living, residence explained 11.2 percent of total variance.

        • KCI등재
        • KCI등재

          생명표기법을 활용한 행복기대여명의 산출

          윤병준 한국보건정보통계학회 2018 한국보건정보통계학회지 Vol.43 No.1

          Objectives: A comprehensive indicator termed happy life expectancy combines age-specific prevalence rates of subjective happiness with objective longevity. The objective of this study was to measure happy life expectancy on Korean with a quality of life index. Methods: The analysis combines age group specific prevalence rates of subjective life satisfaction from a large nationally representative survey and life table estimates of mortality. Employing the period prevalence-rate life table method, Sullivan method. Results: At age group 20-24, men were expected to live 33.07 years of happy life and 34.74 years for women. At age group 40-44, men were expected to live 23.84 years and 26.07years for women. At age group 60-64, men were expected to live 14.11 years and 16.24 years for women in 2016. Conclusions: Different of quality of life measurement could result in substantial differences in happy life expectancy. Multidisciplinary study on quality of life measurement and standardization are needed internationally.

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