The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection

Chun, Yoon-Keun,Ha, Joo-hun,Hong-Jung-Woo,Oh, Soo-Myung,Kim, Sung-Soo 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yoon-Keun Chun¹,Joohun Ha□Hong-Jung Woo□, Soo Myung Oh□,Sung Soo Kim□ ¹Department of Molecular Biology, College of Medicine,²Department of Surgery, college of Medicine,³Department of Internal Medicine, College of Oriental Medicine,and ⁴East-Weat Medical Reserch Institute,Kyung Hee University, Seoul, Korea. The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection. Proceedings of International Symposium on East-West Medicine, Seoul. 212-230, 1999. -Hepatitis B is caused by hepadnavirus. Hepatitis B virus replicates through 3.5kb pregenomic RNA intermediate which is regulated by core promoter. Pathogenesis of hepatitis B virus has been bilieved the result of host immune response. But recently many studies have reported that high level of viral replication caused by mutation in core promoter might result in severs hepatitis. But these studies were performed in vitro, not in vivo. So there is yet debate about which factor, viral of host factor, is more important in pathogenesis of hepatitis B virus. So we measured real viral replication level in 204 chronic hepatitis B patients by quantifying HBV DNA from sera by our novel PCR-based more sensitive method, and compared these results with ALT level measured from same sera, which indicates liver cell damage. Surprisingly there are no significant correlation between HBV DNA quantity and ALT level. Then we cloned core promoter region. In SSCP, we found that many viral mutants coexist in one patient. Base on SSCP result, we chose main viral core promoter type in each patients, which is thought to determine overall viral replication level in this patient. Main type of core promoter region of each 41 patients were directly sequenced. And with these we measured promoter activity by luciferase assay system and compared promoter activity with on another. We found tha there were some differences in promoter activity according to core promoter sequences. And we constructed replication-competent viral constructs with core promoter from 41 patients and Transfected these into HepG2 cell and measured HBV DNA by southern blot. There were also differences in HBV DNA quantity according to core promoter sequences. On these all results we investigated correlation between the effect of HBV core promoter on viral replication in vitro and HBN DNA quantity, ALT level from sera of each patients. We found there is no significant correlation among them. As a result, we concluded that in determining severity chronic hepatitis B patients, host factors of each patient is more important rather than replicative activity of virus itself.

개에서 이중 결찰법을 통한 동맥관 개존증의 완치 예

윤헌영,정순욱,박희명,박철,정만복,김준영,한현정,황민,노병국,박상혁,장하영,박정윤 한국임상수의학회 2004 한국임상수의학회지 Vol.21 No.1

A ten months old, female Yorkshire terrier weighing 2.88 kg referred to veterinary teaching hospital of college of veterinary medicine, Konkuk University because of syncope, cough and dyspnea. First hematological and serum chemical test revealed thrombocytopenia, mild anemia, and increase of concentration of ALP (195 U/L). On 57 days later, second hematological and serum chemical test revealed polycythemia, increase of concentration of ALP (211 U/L), and Tchol (387 mg/dl). Right atrium enlargement, main pulmonary artery bulge and cardiomegaly (VHS = 11.5) were observed in radiographic findings. Ultrasonographic images showed both right and left ventricular dilation and turbulent flow between the descending aorta and the main pulmonary artery in color Doppler imaging. ECG showed left ventricular enlargement, SA block, and electrical alternant. Thoracotomy was performed through left fourth intercostal incision under isoflurane anesthesia. Patent ductus arteriosus was double ligated with 1-0 silk. Cough and dyspnea disappeared on 5 days after operation. Turbulent flow was not found in color doppler imaging of ultrasonography on 10 days after operation. Ten months later after the operation, syncope could not exist any more.

수종의 고정성 보정장치에 따른 유성견의 치주조직 반응

조명훈,윤영주,김광원 대한치과교정학회 2001 대한치과교정학회지 Vol.31 No.1

교정력을 가한 후 각기 다른 재료로 제작된 고정성 보정장치를 적용한 경우에 발생하는 치주조직의 재형성과 치유과정을 조직학적으로 관찰하기 위해 건강한 치주상태를 가진 네 마리의 유성견을 대상으로 최초 교정력이 200gm이 되도록 견인 스프링 (sentalloy closed coil springR, Tomy Co., Japan)을 대상 치아에 결찰하여 1주일 간 교정력을 가한 후, 각각의 실험동물에 3가닥 호선인 0.018인치 DentaflexR(Dentarum Co., Germany), 3가닥 호선인 0.020인치 DentaflexR(Dentarum Co., Germany), 6가닥 호선인 0.0195인치 RespondR(G&H Co., U.S.A.)를, 그리고 자가중합형 레진 접착제인 Superbond C&BR를 고정성 보정장치의 재료를 이용하여 보정장치를 접착한 군과 보정장치를 접착하지 않은 군으로 나누어 3주간 적용 후 희생하여 H-E염색군, M-T염색군으로 나누어 광학현미경으로 관찰하여 다음과 같은 결과를 얻었다. 1.0.0195인치 RespondR를 접착시킨 군은 0.018인치 DentaflexR, 0.020인치 DentaflexR, Superbond C&BR를 접착시킨 군에 비교할 때 압박측에서의 거대세포 침윤 감소와 긴장측에서의 신생골 형성 증가가 매우 두드러지게 나타났으며 치주인대는 형태와 배열에서 대부분 정상적인 소견을 보였다. 2.실험 1군의 모든 실험대상에서 압박측 치조골 내부의 괴사골이 관찰되었고, 압박측과 긴장측 모두에서 치조골 표면의 골양조직 및 sharped 섬유의 형성과 치주인대의 재형성 현상이 나타나는 것이 관찰되었다. 3.실험 2군은 실험 1군에 비교하여 압박측에서 거대세포 침윤이 현저히 감소되었고 치주인대는 거의 정상적인 소견을 보였다. 긴장측에서는 수층의 골침착을 보이며 치주인대 측으로 골양조직과 골아세포가 구상으로 나타나는 활성화 소견을 보였다. 이상의 결과에서 더 여러 가닥이 꼬인 6가닥 호선인 0.0195인치 RespondR(G&H Co., U.S.A.)를 보정장치로 적용한 경우가 다른 재료의 고정성 보정장치보다 더 활발한 신생골주 형성의 활성화 소견이 관찰되었으며 대조군과 유사한 배열과 형태를 보이는 정상적인 치주인대 섬유의 배열양상이 관찰되어 다른 재료들에 비교하여 치주조직의 초기 재형성과정을 더 신속하게 유도하는 것으로 사료된다. The purpose of this study was to evaluate the material for fixed type retainer, allowing physiologic tooth movement. and proper remodeling of periodontal tissue during retention period. The present study was performed to observe the histologic changes of periodontal tissue after application of different types of fixed type retainer after orthodontic tooth movement in young adult dogs. For this study, 4 young adult dogs were used as a experimental animal and experimental group was divided into three groups ; experimental group 1 contained right side maxillary third incisors and canines, experimental group 2 contained contralateral teeth of same animals, and control group contained mandibular premolars. And each dogs were applied the 4 different types of fixed type retainer to experimental group 1. The experimental teeth were ligated on the Sentalloy closed coil springR(Tomy Co., Japan) from maxillary third incisors and canines and applied orthodontic force at initial 200gm-forced during 1 week. All the experimental animals were sacrificed on the 3rd week after the orthodontic teeth movement and then the specimens were taken, fixed in formalin, embeded in parafin, sectioned 6-8μm in thickness and stained with Hematoxylin-Eosin staining, and Masson's trichrome staining method. Examined under the light microscopy. The following results were observed. There were observed that decreased infiltration of giant cells in pressure side and increased the new bone forming in tension side on the specimen of 6-stranded 0.0195' RespondR(G&H Co., U.S.A.) group. Periodontal ligament fibers were much compressed or elongated in 3-stranded 0.018', 0.020' DentaflexR(Dentarum Co., Germany), and Superbond C&BR(Sun Medical Co., Japan) groups. 2. In experimental group 1, necrotic bone inside the alveolar bone of pressure side, forming of the sharpey's fiber in osteoid tissue, and remodeling of the periodontal ligament were observed in all animals. 3. In experimental group 2, it was observed that the amount of bone resorption was equal or decreased in pressure side, and increased new bone forming and significantly decreased Infiltration of giant cell than the experimental group 1. By this results, it considered that 6-stranded RespondR(G&H Co., U.S.A.) wire was the most useful material allowing early periodontal tissue remodeling.

임신 7 개월 이후 임산부 보행의 역학적 분석

금명숙,유실,김영란,정남주,한윤수,이훈표,윤희중 한국운동역학회 2002 한국운동역학회지 Vol.12 No.1

The purpose of this study was analyzed the effect of kinematical and kinetical factors of lower extremity of form change in the cause of growth an unborn child during in pregnancy. Three pregnant women were selected from pregnant 24 weeks as subjects. Each subjects were required to walk with usual walking speed. Cinematographic and GRF data were collected during walking, and the kinematical and kinetical variables were calculated using Kwon3d. Based on the results of the study, the following conclusions were drawn : 1. Step width and Step length Step width according to the period of pregnancy was gradually small but step length was gadually great. 2. Angle of lower extremity The angle of hip, knee and ankle was differed pregnancy on 9 month and other time. 3. Ground reaction force In the three part of ground reaction force, pregnancy on 9 month was most greater than other time.

중추신경계에 침범한 속립성 결핵 : 증례보고

이태환,김헌주,허철,한용표,홍순기,변진수,김명순,조미연,김윤미 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.11

in recent times, central nervous system(CNS) tuberculosis has been rare and the prevalence of the focal form, the tuberculoma, varies from 1 per 200 to 1 per 1000. CNS tuberculosis occurs as a result of hematogenous spread from a primary focus. mostly pulmonary tuberculosis. It can be diffuse exudative leptomeningitis or a localized tuberculoma ; the former is more common. We report a case of miliary cerebral tuberculosis in a 24-year-old female who had been having headaches for 4 months. Magnetic resonance imaging showed numerous small round Gadolinium-enhanced supratentorial and infratentorial lesions scattered throughout the brain. Histologic examination confirmed well defined tuberculous granulomas with central caseous necrosis in open biopsy, containing several acid-fast bacilli, the patient was treated with isoniazid, rifampin, ethambutol, pyrazinamide in combination with prednisolone.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

Glaucoma diagnostic value of the total macular thickness and ganglion cell-inner plexiform layer thickness according to optic disc area

Yoon, Myung Hun,Park, Sang Jun,Kim, Chan Yun,Chin, Hee Seung,Kim, Na Rae British Medical Association 2014 British journal of ophthalmology Vol.98 No.3

<P><B>Aims</B></P><P>This study examined the diagnostic value of the perimacular ganglion cell-inner plexiform layer (GCIPL) thickness in the detection of glaucoma according to the optic disc area.</P><P><B>Methods</B></P><P>The participants underwent reliable standard automated perimetry tests and Cirrus optical coherence tomography. The area under the receiver operating characteristic curve (AUC) was used to determine the diagnostic ability of the GCIPL, total macular thickness, retinal nerve fibre layer (RNFL) and optic nerve head parameters. Subsequently, optic disc areas of ≤1.9, ≤2.4 and >2.4 mm<SUP>2</SUP> were chosen arbitrarily, and the AUCs and sensitivities at fixed specificities were compared for each.</P><P><B>Results</B></P><P>87 normal subjects and 96 glaucoma patients were enrolled. The mean GCIPL thickness and RNFL showed similar ability to diagnose glaucoma (p=0.911). The mean GCIPL thickness had higher diagnostic ability than the central retinal subfield thickness (p=0.001), comparable with that of the retinal cube volume (p=0.840) and mean retinal cube thickness (p=0.840). The mean RNFL (0.866), the minimum GCIPL (0.840) and the inferior and inferotemporal (0.853) disc had the highest AUCs for small, medium and large discs, respectively.</P><P><B>Conclusions</B></P><P>The macular GCIPL thickness, total macular cube thickness, circumpapillary RNFL thickness and disc rim area showed similar performance in diagnosing glaucoma, irrespective of the optic disc size.</P>

Impaired Diastolic Recovery after Acute Myocardial Infarction as a Predictor of Adverse Events

Yoon, Hyun Ju,Kim, Kye Hun,Kim, Jong Yoon,Cho, Jae Young,Yoon, Nam Sik,Park, Hyung Wook,Hong, Young Joon,Kim, Ju Han,Ahn, Youngkeun,Jeong, Myung Ho,Cho, Jeong Gwan,Park, Jong Chun Korean Society of Echocardiography 2015 Journal of Cardiovascular Imaging (J Cardiovasc Im Vol.23 No.3

<P><B>Background</B></P><P>To investigate the impact of left ventricular (LV) diastolic functional recovery on major adverse cardiac events (MACE) 6 months after acute myocardial infarction (AMI) in patients with preserved LV systolic function.</P><P><B>Methods</B></P><P>A total 463 patients with preserved LV systolic function at 6 months after an AMI were divided into two groups based on the extent of diastolic recovery assessed by echocardiography: group I (n = 241) showed improving diastolic function and group II (n = 222) did not. MACE included death, recurrent myocardial infarction, and rehospitalization due to heart failure, and these events were compared with the patients' characteristics at baseline.</P><P><B>Results</B></P><P>Compared with group I, the patients in group II were older and had a higher prevalence of hypertension and diabetes. Blood levels of hemoglobin and triglyceride were lower in group II, whereas the levels of N-terminal pro-B-type natriuretic peptide (NT-proBNP) and of high-sensitivity C-reactive protein were higher in this group than in group I. MACE were significantly more frequent in group II than in group I. Age, elevated NT-proBNP, and impaired diastolic recovery were significant independent predictors of MACE.</P><P><B>Conclusion</B></P><P>Despite improvement in LV systolic function, LV diastolic function had not improved in 222 patients (47.9%) by the 6-month follow-up after the index AMI, and impaired diastolic functional recovery was found to be an independent predictor of MACE. Evaluation of diastolic function would be a useful way to stratify risk in patients discharged after an index AMI.</P>

비디오 영상 예측과 악교정수술

조명훈(Myung Hun Jo),김동훈(Dong Hun Kim),김중한(Joong Han Kim),지국섭(Kuk Sop Chi),박성만(Seong Man Park),윤영주(Young Jooh Yoon),김광원(Kwang Won Kim) 조선대학교 구강생물학연구소 2000 Oral Biology Research (Oral Biol Res) Vol.24 No.1

.