Carbon monoxide prevents TNF-α-induced eNOS downregulation by inhibiting NF-κB-responsive miR-155-5p biogenesis

Choi, Seunghwan,Kim, Joohwan,Kim, Ji-Hee,Lee, Dong-Keon,Park, Wonjin,Park, Minsik,Kim, Suji,Hwang, Jong Yun,Won, Moo-Ho,Choi, Yoon Kyung,Ryoo, Sungwoo,Ha, Kwon-Soo,Kwon, Young-Guen,Kim, Young-Myeong Nature Publishing Group 2017 Experimental and molecular medicine Vol.49 No.11

<P>Heme oxygenase-1-derived carbon monoxide prevents inflammatory vascular disorders. To date, there is no clear evidence that HO-1/CO prevents endothelial dysfunction associated with the downregulation of endothelial NO synthesis in human endothelial cells stimulated with TNF-α. Here, we found that the CO-releasing compound CORM-2 prevented TNF-α-mediated decreases in eNOS expression and NO/cGMP production, without affecting eNOS promoter activity, by maintaining the functional activity of the <I>eNOS</I> mRNA 3′-untranslated region. By contrast, CORM-2 inhibited MIR155HG expression and miR-155-5p biogenesis in TNF-α-stimulated endothelial cells, resulting in recovery of the 3′-UTR activity of <I>eNOS</I> mRNA, a target of miR-155-5p. The beneficial effect of CORM-2 was blocked by an NF-κB inhibitor, a miR-155-5p mimic, a HO-1 inhibitor and siRNA against HO-1, indicating that CO rescues TNF-α-induced eNOS downregulation through NF-κB-responsive miR-155-5p expression via HO-1 induction; similar protective effects of ectopic HO-1 expression and bilirubin were observed in endothelial cells treated with TNF-α. Moreover, heme degradation products, except iron and <I>N</I>-acetylcysteine prevented H<SUB>2</SUB>O<SUB>2</SUB>-mediated miR-155-5p biogenesis and eNOS downregulation. These data demonstrate that CO prevents TNF-α-mediated eNOS downregulation by inhibiting redox-sensitive miR-155-5p biogenesis through a positive forward circuit between CO and HO-1 induction. This circuit may play an important preventive role in inflammatory endothelial dysfunction associated with human vascular diseases.</P>

원위 대퇴골의 고립성 골연골종에서 발생한 속발성 연골 육종 1례 보고

정필현,황장수,강석,김용민,오형호,채동주,김종필 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

연골육종은 비교적 서서히 자라며 늦게 전이하는 악성 연골 종양으로 알려져 있다. 골 연골종에서 발생한 속발성 연골육종은 발생빈도는 드물며 타부위로의 전이가 적고 악성도가 낮은 경우가 많아 적합한 수술적 치료로서 좋은 예후를 보이는 것으로 보고되고 있다. 본 동국대학교 정형외과학 교실에서는 우측 대퇴골 원위부 골간단부에 생긴 고립성 골 연골종에서 이차적으로 발생한 연골육종을 치험한 바 문헌고찰과 함께 보고하는 바이다. A case of Secondary Chondrosarcoma Arising from Solitary Osteochondroma of the Distal Femur Department of Orthopaedic Surgery, College of Medicine, DongGuk University Phil Hyun Chung M.D., Jung Su Hwang M.D., Suk Kang M.D., Yang Mln Kim M.D., Hyung Ho Oh M.D., Dong Ju Chae M.D., Jong Pil Kim M.D. Chondrpsarcoma is a malignant cartilage-forming tumor that grows slowly and metastasis of this tumor occurs in late stage. Secondary chondrosarcomas arising from a solitary osteocartilaginous exostosis are rare and those have a better prognosis than other chondrosarcomas, and they rarely metastasize. We present our experience with a case of secondary chondrpsarcoma arising in solitary osteochondroma of distal metaphysis of right femur with clinical details.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

니켈-카드뮴 蓄電池중의 니켈極의 特性에 미치는 電解質의 影響

黃正儀,鄭倧在,金昌鎬 慶北大學校 自然科學大學 1986 自然科學論文集 Vol.4 No.-

Properties of the Ni(OH) electrode in Nickel-Cadmium battery are studied by the cyclo-voltammetric method in base solutions (LiOH, NaOH, KOH)at 20℃. The nickel electrode was prepared chemical precipitation of Ni(OH)_2 on the conducting substrate. The redox current ratio(ipa/ipc) which is closely related to the charge ratio of the Ni(OH)_2 electrode is greater than unity. We can conclude that OH^-ions have been inserted into the Ni(OH)_2 intersheets or adsorbed on the Ni(OH)_2 layers. The electrooxidation of inserted or adsorbed OH^- ions can be explained through a catalytic process involving the participation of Ni(Ⅱ)→Ni(Ⅲ) electrochemical reaction. The reaction products such as (OH) or OH^- also were retained in the defects of Ni(OH)_2 crystalline layers. The redox current ratio is increased by the existence of molecular oxygen in the electrolyte, because the number of OH^- ion in IHP may be increased by reduction of molecular oxygen. Voltammograms run in LiOH solutions are comparable to those recorded in NaOH or KOH solutions. In the same perturbation conditions, redox current ratio related to the Ni(Ⅱ)/Ni(Ⅲ) redox couple values in LiOH are greater than those obtained in NaOH or KOH. The effect of LiOH solution may be ascribed to the presence of highly solvated cations Li^+. In order that OH- ions can be closely adsorbed on the electrode surface, chemical work must be done to take off some water molecules from its primary hydrated sheath. It is guessed that solvated cations take up these chemical works. The sequence of redox current ratio is Li^+>Na^+>K^+.

소디움 루프 운전지원시스템

황종선,남호윤,문성익,이광형,조충호 한국경영과학회 1996 한국경영과학회 학술대회논문집 Vol.- No.1

본 연구에서는 액체금속로의 핵심구조인 소디움루프를 위한 운전지원 전문가시스템을 개발한다. 소대움루프는 현재 원자력연구소에서 연구 개발 단계에 있으며, 안전 운전을 위한 운전 지식을 연구하고 있는 중이다. 본 논문에서는 이러한 연구 상황에 맞추어 개발되는 전문가시스템에게 요구되는 특징을 정리하고, 소디움루프 운전의 효과적 지원과 운전원의 편의에 중점을 둔 전문가시스템의 개발을 설명한다.

電子製品經路에서 權力과 葛藤에 관한 硏究

황호종 용인대학교 산업경영연구소 1996 산업경영논총 Vol.1 No.-

Marketing channels have been characterized as social systems by the recent channel literatures. Under this assumption, many channel behavirol theories were developed by examining hypothetical relationships among several channel variables Among them, interrelationship between channel power and conflict has been one of major concerns to channel managers. From this point of view, the objecive of this paper is to search the relationship between power and conflict in the franchise system of electronic goods. In order to realize our goal, data was collected from home elctronics retailers and a simple random sample of 150dealers was drawn and tested. Major findings through the data analyses are as follows; 1.Coercive sources of power are increasing the conflict level of the dealers. On the contrary, noncoercive sources of power tends to reduce intrachannel conflict between dealers and manufactures. 2. Channel conflict negatively affects dealers' performance measured by perceived satisfaction. 3. Noncoercive sources power improve channel performance.

포괄수가지불제도 적용에 따른 산부인과 진료수입의 변화

황태연,유병철,정귀언,정수진,김성준,배기택,손혜숙,이종태,전진호,엄상화 인제대학교 2001 仁濟醫學 Vol.22 No.1

Objectives: Under the fee-for-service system, Korean hospitals have been suffering from the worsening profitability caused by law medical care fees. To overcome the difficulties, they have maximized the quantity of medical services and made relatively large investment in developing new medical services using high-priced medical equipment, medicines and medical supplies which were not covered by medical insurance system rather than reduced running costs. Materials and Methods: This study was made to analyze and forecast the revenue change of the department of Obstetrics and gynecology of an university hospital in Seoul, expecting the implementation of DRG system in 2000. Results: The results were as follows : 1.During the first two days of hospitalization. 41% of total medical service fee was occurred, which was thought that most of the check-ups and operations were made during this period. After two days of hospitalization, the new occurrence of medical service fee tended to reduce. 2.Out of total medical service fee, 67% of admission fee was occurred after 5 days of hospitalization. This was because medical service fees in obstetrics and gynecology department occurred during the first 5 days of hospitalization. 3.Out of fees for operation, treatment, medical supplies and blood transfusion. 71% was occurred during the first two days. 4. In case of fees for examination, 50% was occurred during the first two days. 5.A total of 53% of fees for medication and injection was occurred during the first 5 days. Conclusions: By the implementation of the DRG system, the income is forecast to increase by 800 won to 310,000 won by the disease group of obstetrics and gynecology department. To increase hospital income with the implementation of DRG system, the results of this study suggest that examinations should be done at out-patient departments before the hospitalization of patient, the discharge of patient should be noticed in advance to reduce the period of hospitalization, and admission appointment system should be implemented for the immediate operation and delivery.

株價指數先物의 헤징效果 分析

黃豪鍾 동국대학교 경영관리연구소 1992 經營經濟論叢 Vol.16 No.-

In early 1992, the domestic capital maket became partially liberalized and unified with the international capital market. In consequence, it is apparent that the market volatility will be much more increased than ever before on account of the inflow and outflow of foreign capital into the Korean market. In order to hedge these financial risks, especially for the fluctuation of the stock price, new investment techniques should be developed and introduced into the Korean stock market. Stock Index futures is an advanced risk hedging technique which removes the systematic risk from the stock portfolio owned by all kinds of stock investors. Therefore, the objective of this study is to examine the hedging effect of the stock index futures by assuming that it has been introduced into Korea. Main research method in this study is focused on the selection of hedging models, the date of maturity of future contracts and hedging ratio. To examine the hedging effectiveness of stock index futures in the Korean stock market, we have calculated the risk and return combinations which could have been achieved by selling index futures(Han Gyung Dow Average) against the Composite stock index for the one week holding period. The sample period was from January, 1989 through June, 1992. From the results of tentative analysis, we can have four findings as follows : 1. As for the hedging effect, minimun risk hedging models have comparatively higher hedging effects than those of the date hedging model. 2. Hedging effects were not differentiated by the date of maturity for two models. 3. Though all hedging ratios are smaller than 1, they are almost same as 1. 4. The beta model showed better hedging effects in the decling period of stock price than in the rising period of the stock price.

유통경로성과의 유형과 측정에 관한 연구

황호종 용인대학교 산업경영연구소 1998 산업경영논총 Vol.3 No.-

The purpose of this study is to classify the determinants of channel performance and to reveal the process of measuring performance of individual channel members. Three approches are used to classify the determinants of channel performance as fellows: channel system as a whole vs. individual channel members, Behavior-based vs. Outcome-Based, and positivistic vs. ecological approach. The first approach includes two factors of effectiveness and efficiency as determinants of channel system performance. The second one measures performance with several factors such as degree of channel coordination, cooperation and conflict etc. The ecological approach selects three factors: productivity, effectiveness, and fairness of the channel system. Based on these several approches, we can introduce the process of measuring performance of individual channel members. The first step is to describe the general nature of performance. The second step specifies the focal domain being evaluated. The third step is to identifies the performance assessment perspectives. The fourth step is to establish the type of data required. The fourth step generates items for the performance scale. Through these process, appropriate framework can be developed to measure the performance by researchers.

2상 스테인리스강(STS 329J1)의 크리프 특성

황경충,권종완,윤종호 한국공작기계학회 2005 한국생산제조학회지 Vol.14 No.2

Micro duplex stainless steel is used to denote a fine scale two-phase micro structure consisting austenite and ferrite. The development of this structure was done by proper thermo-mechanical processing. The objective of present investigation is to study creep characteristics of this alloy. Since we have little design data about the crap behaviors of the alloy. An apparatus has been designed and built for conducting creep tests under constant load conditions. A series of creep tests on them have been performed to get the basic design data and life prediction of micro duplex stainless steel products and we have gotten the following results. First, the stress exponents decrease as the test temperatures increase. Secondly, the creep activation energy gradually decreases as the stresses become higher. Thirdly, the constant of Larson-Miller parameters on this alloy is estimated as about 5. Last, the fractographs at the creep rupture show both the ductile and brittle fracture modes according to the creep conditions.