Identification of a Gene Cluster in Streptomyces spectabilis-spectinomycin producer

오태진,김형준,송재경 선문대학교 대학원 2000 대학원학술논문집 Vol.2 No.-

Spectinomycin (Spc) is an aminoglycoside antibiotic which shows remarkable broad-spectrum. From a chromosomal cosmid library of Streptomyces spectabills, a Spc producer, a clone (pSPM8) was isolated by homology to the dTDP-glucose 4,6-dehydratase and aminotransferase genes which obtained from S. spectabilis genome using a polymerase chain reaction. From this clone, a 5.8-kb DNA region was sequenced and found to encode four open reading frames and one Incomplete reading frame. The putative orfl protein reveals 29% amino acid sequence homology of putative myo-inosltol-2-dehydrogenase from Vibrio anguillarnm. The orf2 is about 46% homologous to putative myo-inositol-1-monophosphotase of Mycobacterium leprae. The orf3 showed strong homology (67.4%) to putative export protein (spcT) from S. flavopersicus. The amino acid sequence deduced from 0114 showed a high similarity (70.8%) to a spectinomycin resistance gene (spcN) from S. flavoperslcus. Expression of orf4 in E. coti BL21 showed the spectinomycin resistance.

Schottky Diode의 특성과 CSTL

오상식,김재형 인제대학교 1983 仁濟醫學 Vol.4 No.4

This paper described the characteristics of Schottky diode and Complementary Schottky Transistor Logic (CSTL) with Schottky diode. The Schottky barrier heights of Al and Al-2%Si layers to n-type Si were measured using I-V measurement and their values were 0.67eV and 0.85eV, respectively. To measure ac characteristics of CSTL, 29-stage ring oscillator and T-flip flop had been designed and fabricated according to Standard bipolar Process. For Al and Al-2%Si Schottky contact, the minimum propagation delay time and the maximum toggle frequency were measured to be 5ns, 10ns, 22MHz and 16MHz, respectively.

In Vitro 고삼투압이 정자 원형질막의 Protein Tyrosine Phosphorylation에 미치는 영향

오영근,장재호,최인호,정노팔,신형철,곽병주 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.4

정자의 원형질막은 삼투압에 의해서 영향을 받는다고 보고되고 있다. 이중 세포막내 분자구조의 변화 특히 막지질 구조의 변화와 동반되는 이온채널의 변화 그리고 Ca2+과 HCO-3의 유동성과도 밀접한 관련이 있는 것으로 보고되고 있다. 지금까지의 연구보고에 의하면, 정자의 첨체반응 (acrosome reaction)이 일어날 경우 protein tyrosine phosphorylation이 증가되는데 이것은 cAMP, protein kinase A 등을 통하여 작용되는 것으로 보고되고 있다. 막의 지질변화를 유도하는 물질로 일종의 sterol acceptor인 BSA가 알려져 있는 바, 실제로 BSA가 막지질 성분에 미치는 영향을 관찰한 결과 cholesterol이 유출되고 이온 등의 유동성 변화가 일어나, 이 유동성 변화가 정자의 adenylyl cyclase를 활성화시켜 cAMP를 증가시키고, PKA가 활성화되어 결과적으로 Protein tyosine phosphorycation이 유도된다고 보는 것이다. 침체반응과 protein tyrosine phosphorylation과는 밀접한 관계가 있는 것으로 사료되고 있다. 본 연구는 정자 원형질막에서 cholesterol이 유출되어 protein tyrosine phosphorylation이 유도될 때, BSA와 같은 sterol acceptor가 작용할 것이라는 전제하에, 고삼투압 하에서 탈수로 인해 원형질막이 위축되더라도 sterol acceptor가 존재한다면 막지질 성분의 구조적 변화가 억제 될 수 있을 것이라는 가설을 설정하였다. 실험결과, 저온 및 고삼투압 하에서 정자운동은 감소되지만 원형질막의 구조적 변화는 없고, 삼투압에 대한 반응정도는 원형질막을 통한 수분이동과 세포용적 변화에 따라 비례적으로 일어난다고 하는 사실을 발견하였다. 이 결과는 정자보존에 있어서 저온변화에 영향을 미치는 여러 인자들 특히 protein fgrosine phosphorylation의 증가와 밀접한 관계가 있음을 시시해 준다. 또한 sterol acceptor로 알려진 BSA는 삼투압이 변화되더라도 역시 중요한 인자로 작용할 수 있음을 알 수 있었으며, 특히 고삼투압으로의 변화는 cAMP와 protein kiname A를 거치는 신호전달과정에 있어서 중요한 요인이라는 사실을 확인할 수 있었다. It has been reported that plasma membrane activity of the spermatozoa may be susceptible to be influenced by extracellular osmolality and such membranous changes involve intracellular molecular changes, special regard to the structure of membranous lipids, and the accompanying ion-channel of which are closely related with their fluidity of Ca2+ and HCO-3. It is of common recognition that a certain kind of sterol acceptor player an important to induce lipid fluctuation of the sperm plasma membrane which have been influenced by BSA administration and came in effect to outflow of cholesterol from the spermatozoa and resulted in changes of ionic fluidity to facilitate adenylyl cyclase, and to induce protein tyrosine phosphorylation by increase of cAMP and activation of PKA. Thus it seems likely that an augmentation of the acrosomal reaction is closely related with protein tyrosine phosphorylation. The following experimental results were obtained in the present study; Under the high osmolality conditions, the spermatozoa motility declined significantly and the structural change of the plasma membrane diminished to confirm that the response degrees to the osmolality depended upon the water transfer volume through the plasma membrane and the changes of cellular volume. Those experimental results suggest that a physiological parameter such as low temperature condition played an important role for presentation of spermatozoa and that inducement of spermatozoa activation for reinforcement of protein tyrosine phosphorylation. On the other hand, it seemed likely that the BSA administration as one of sterol acceptors might represent a key role also under the high osmolality condition and their result also suggests that osmolality change, special regard to high osmolality condition may play an important role also in the processes of signal transmission

2차원 Si MOSFET 시뮬레이터 개발

오형재,박성호 서울市立大學校 1989 論文集 Vol.23 No.-

In this paper, a two-dimensional Si MOSFET simulator has been developed. The drift diffusion model is adopted as the physical basis in our simulator, and hence it is only applicable to Si MOSFET's with gate length above 0.5㎛. In this simulator, the basic differntial equations are discretized by the control volumn method and the matrix equations are solved by SIP method. The Si MOSFET with 1㎛ gate length has been analyzed by use of our simulator. The potential and electron distributions were presented and the drain current-voltage characteristics was calculated. The results of characteristics turned out fairly fitted to the results derived from MINIMOS.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

가성부갑상선 기능저하증 환아의 구강 증상 : CASE REPORT

김성오,홍은경,최형준,이제호,손흥규 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.2

부갑상선 호르몬은 혈중 인농도를 낮추고, 칼슘농도를 증가시키는 호르몬으로 부갑상선기능저하증은 부갑상선이 존재하지 않거나 비정상적으로 기능하여 이 호르몬의 분비가 감소되는 질환이며, 가성부갑상선기능저하증은 부갑상선 호르몬 분비는 정상이나, 이 호르몬에 대한 괄과 신장의 반응이상으로 혈중 칼슘 농도가 감소되고 인농도가 증가되는 질환이다. 가성부갑상선기능저하증은 임상적으로 성장 및 발육부전, 둥근 얼굴, 조기 골단폐쇄로 인한 단지증, 이소성 연조직 석회화, 비만, 두개관의 비후, 정신지체, 백내장 등이 나타난다. 구강내 소견으로는 치아의 맹출지연, 법랑질형성부전이 가장 큰 특징이며, 그 외에 결손지, 부정교합, 높은 구개궁, 확장된 치근관과 짧은 치근, 치수강 내 석회화, 높은 우식 이환율, 치근막의 비후 등이 관찰된다. 본 증례에서 환아의 구강내 소견은 맹출한 하악 4전치와 상악 좌우측 영구 중절치, 상악 우측 영구 측절치, 상, 하악 제 1대 구치에서 법랑질형성부전을 보였고, 다수의 유치에서 심한 동요도를 보였다. 방사선 소견으로는 대부분의 미맹출된 영구치들도 법랑질형성부전이 관찰되었으며, 특히 영구 견치의 경우 심한 치관의 형태이상을 볼 수 있었다. 또한 유치의 비정상적인 치근흡수가 나타났고, 하악 전치의 경우 치근형태가 짧고, 치근단이 둥근 형태로 나타났다. 이에 저자는 가성부갑상선기능저하증인 환아의 구강내 소견에 대해 보고하고자 한다. The parathyroid hormone plays a major role in the metabolism of calcium and phosphorus. In hypoparathyroidism the parathyroid glands are atrophied or absent associated with autoantibodies against parathyroid tissue. Pseudohypoparathyroidism is a metabolic disease caused by the disturbance in peripheral action of parathormone, but parathormone level is normal. In general, patients with pseudohypoparathyroidism have short stature, round face, brachydactylia, obesity, mental retardation, cataracts & ectopic calcifications on soft tissues. Dental manifestations are enamel hypoplasia, delayed eruption, blunting of root apex, hypodontia, pulp calcification, thickened lamina dura, excessive caries & malocclusion. In this case, intraoral examination showed enamel hypoplasia on the erupted permanent teeth & hypermobility on the remaining deciduous teeth. From the radiographic view, severe dental anomalies were observed on canines and shortening and blunting of root apex was observed on mandibular incisors. Pathologic root resorption was also observed on deciduous teeth.

Cds-(As-Te-Ge) 非晶質 Heterojunction

李亨宰,李用五 全北大學校 文理科大學 1976 論文集 Vol.4 No.-

Heterojunctions between CdS and As_3-Te_6-Ge_1 amorphous films have been fabricated by vacuum evaporation in order to investigate the electrical properties of the junctions. Excellent current rectification is obtained in lower applied voltages, while symmetric Ⅰ-Ⅴ characteristics is shown in higher voltage. Under forward bias, the electrode contact to the CdS film is made negative with respect to metal-(As-Te-Ge) junction. The electrical properties are characterized by junction characteristics in lower voltage, and by bulk characteristics in higher voltage. The junction shows asymmetric switching characteristics which has not been observed in any other devices.

응급센터에서 기관내 삽관을 시행하지 않은 호흡곤란 환자의 동맥혈 이산화탄소분압과 호기말 이산화탄소분압의 연관성분석

김형국,박승현,오동렬,박규남,이원재,황두영,최승필,이운정,정시경,김세경 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.4

Background: The end-tidal carbon dioxide tension(ETCO₂) is defined as a partial pressure of carbon dioxide at the end of an exhaled breath. The purpose of this study is to determine the correlation between ETCO₂and arterial carbon dioxide tension(PaO₂) in nonintubated patients with respiratory distress in emergency department(ED). Methods: A prospective non-blind study was performed in ED of our university hospitals. Participants included all nonintubated adult patients with respiratory distress requiring arterial blood gas analysis. ETCO₂was measured with a capnography monitor during tidal volume breathing. ETCO₂were recoreded at the time of arterial blood gas sampling. The correlation between ETCO₂and PaCO₂was analyzed in all patients and in subgroups by simple linear regression. Results: Sixty patients were enrolled. In all patients, ETCO₂was 5.72mmHg lower than PaCO₂and correlated well with PaCO₂(r²=0.716). ETCO₂correlated best with PaCO₂in patients who were either acidotic or non-smoking. Conclusion: ETCO₂correlate well with PaCO₂in nonintubated patients with respiratory distress in ED. ETCO₂may be sufficient to reflect PaCO₂in selected patients and obviate the need for repeat arterial blood gas determination.

아파트의 固體振動 低減對策에 관한 硏究

이주형,오제응 漢陽大學校 環境科學硏究所 1988 環境科學論文集 Vol.9 No.-

오늘날의 住居空間은 生活文化가 發達하고 變化함에 따라 經濟的이고 便利하여 졌으나, 騷音環境側面에서 불 때 적합하지 않은 문제점도 發生하게 되었다. 즉 騷音環境에 대한 要求가 더욱 세심해지고 住居空間의 構造變化에 따라 Wall Slab 아파트에서 structure-borne sound와 vibration이 심각한 문제로 제기되었다. 하지만 이에 대한 규제나 基準이 없는 실정이어서 生活文化發達에 부응하는 住居環境의 達成이 시급하다. 本 硏究에서는 Wall Slab구조 아파트에 대한 structure-borne sound와 vibration의 실상을 파악하기 위해서 "建築物의 現場에 있어서의 바닥 충격레벨의 測定方法"에 의해 소음과 진동을 측정하였다. 이에 대한 結論은 1) Wall Slab 와 R.C조를 비교한 결과 500㎐이하에서 약 10㏈의 차이를 보이면서 R.C조가 충격음과 진동에서 좋았다. 2) structure-borne sound와 vibration를 저감시키기 위해 Life Slab의 채택이 필요할 뿐만 아니라, 50~65m/m정도에 Life Slab는 가장 효율적인 節緣値를 나타냈다. The purpose of this study is to be calculated the problems of the Structure-bone sound and vibration to a apartment mode wall slab type and to be made the counterplan of noise reduction Actually based on respond to a resident, Structure-bone sound and vibration is measured with "Method for field measurement of floor impact sound level" The result of this study figures out: 1) The apartment mode reinforce concrete compared to the apartment made wall-siab has a good condition to a Structure-bone sound and vibration, taken the 10㏈ difference under the 500㎐. 2) Needed life-slab to apartment construction for the noise reduction of a apartment Structure-bone sound and vibration, having efficiently the 50~65m/m of life-slab increasing the degree of strength with a interior power wall within 5.Om of span in living space.

저장용액의 온도에 따른 치주인대세포의 생존율

조재현,김성오,최형준,이제호,손흥규,최병재 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.1

외상성 손상 후 치아 탈구 시 치아를 저장하는 저장용액의 종류와 온도가 치주인대세포의 생존율에 어떠한 영향을 미치는지 연구하기 위하여 치주인대세포를 10% fetus bovine serum(FBD) 함유 α-minimal essential medium(α-MEM)에서 37℃ 5% CO_(2) 공기 혼합 배양기에서 배양하고 4.25, 37℃의 Hank's balanced sa1t solution(HBSS)과 α-MEM, 우유(S회사, P회사), tap water에 저장하고 60분이 지난 후 각 군에 대해 치주인대세포의 생존율을 측정하기 위하여 MTT assay를 시행하여 다음과 같은 결과를 얻었다. 1. 4℃ 저장용액의 치주인대세포의 생존율은 α-MEM과 P회사우유에서 가장 높았고 HBSS, S회사우유, tap water 순으로 낮았다. 2. 25℃ 저장용액의 치주인대세포의 생존율은 α-MEM에서 가장 높았고 P회사우유, HBSS, S회사우유, tap water 순으로 낮았다. 3. 37℃ 저장용액의 치주인대세포의 생존율은 α-MEM과 P회사우유, HDSS, S회사우유에서 높았고 tap water에서 가장 낮았다. 4. α-MEM의 치주인대세포의 생존율은 4℃에서 가장 높았고 25℃와 37℃ 순으로 낮았으며 HBSS에서는 4℃에서 높았고 25℃와 37℃에서 낮았다. 5. S회사와 P회사우유에서 치주인대세포의 생존율은 4℃와 25℃에서 높았고 37℃에서 낮았다. 이상의 결과를 종합해볼 때 외상으로 인해 치아가 탈구되었을 때 탈구된 치아의 저장용액으로 HBSS 용액이 추천되고 있으나 이 연구에서 4℃와 25℃ 우유에서 치주인대세포의 생존율이 높았으므로 사고 현장에서 쉽게 구할 수 있고 치주인대세포의 생활력 보존에도 유리한 낮은 온도의 우유에 탈구된 치아를 보존하는 방법도 좋을 것으로 생각된다. To compare the survival rate of periodontal ligament cells preserved in storage media with good availability at the time of an avulsion injury, periodontal ligament cells were incubated in α-MEM culture medium containing 10% FBS in condition of 37℃, 5% CO_(2). These cells were then cultured in HBSS, α-MEM, milk(S. co., P. co.) and tap water at the temperature of 4, 25, 37℃ each in 60 min. The groups were measured by MTT assay. The results were as follows : 1. Among the storage media at 4℃, α-MEM and P-milk had the highest preserving ability of periodontal ligament cells, while that of HBSS, S-milk and tap was low in order. 2. Among the storage media at 25℃, α-MEM had the highest preserving ability of periodontal ligament cells, while that of P-milk, HBSS, S-milk, tap water was low in order. 3. Among the storage media at 37℃, the preserving ability of periodontal ligament cells was very high in α-MEM, P-milk, HBSS and S-milk. it' s lowest in tap water. 4. The preserving ability of periodontal ligament cells in α-MEM was high at 4℃ and it’s low in order of 25℃, 37℃, but in HBSS was high at 4℃ and it's low at 25℃, 37℃. 5. The preserving ability of periodontal ligament cells in S-milk and P-milk was high at 4℃, 25℃ and it’s low at 37℃. In conclusion, HBSS is the storage medium of choice in an avulsion, but in this study it is preferable to choose milk at 4℃ for tooth since it is easy to get and affect cell viability.