파 雄性不稔性의 遺傳分析 : Allium fistulosum L.

宋基現,金鎭漢 忠北大學校 農業科學硏究所 2002 農業科學硏究 Vol.19 No.-

Mlae streilith is one of the major tools for F1 Hybrid seed production in vegetable. Until 1980s, the F1 hybrid seed production in welsh onion (Allium fistulosum L) was accomplished by using genic male sterile lines However, the 50% male fertile plants had to be elminated from the seed production field by hand. In 1989, a new male sterile mutant (MS216) was found on the Breeding and Research Station of Hungnong, which is assumed for cyto;lasmic male sterilty. This experiment was performed to study on the inheritance of male sterility in MS216 The inheritance of male sterility in MS216 was analyzed by determining segregation ratio obtainde from crossings with MS216 and male fertile lines or GMS line. F1,BC1 and BC2 were taken from back-crossing with MS216for recipient and 5 different amle fertile lines for ercurrent In the same way F1, BC1 and BC2 by crossing with MS216 for recipient and 2 different GMS lines for recurrent was obtainde All plants of the F1, BC1 and BC2 population derived from the crossing with MS216 and 5 different male fertile lines showde male sterility in the field, suggesting that the MS216 has a factor influencing male sterility in its cytoplasm And also, from the experiment of crossing between the MS216 and the two GMS lines it was found that genic factor in the GMS lines was not related to the male sterility of MS216 These results show that MS216 has typical cytoplasmic male sterility not affected by any karyogene

두부방사선규격사진 촬영 시 유도방법에 따른 자연두부자세의 차이 및 재현성에 관한 연구

송진명,이기헌,,황현식 대한치과교정학회 2005 대한치과교정학회지 Vol.35 No.5

두부방사선규격사진에서는 매 촬영 시마다 두부의 수직적 회전 정도를 일정하게 유지하기 위하여 두부자세의 재현이 필요하다. 본 연구는 유도방법에 따른 자연두부자세의 수직적 두부회전 정도의 차이를 비교하고 각 두부자세의 재현성을 평가하기 위하여 시행되엇다. 20대 성인 남녀 30명을 연구대상으로 하여 head posture allgner를 이용한 방법(HPA법)과 self balance posture를 이용한 방법(SBP법)으로 자연두부자세를 유도하여 측모 및 정모두부방사선규격사진을 각각 한 달 간격으로 촬영한 후, 측모두부방사선규격사진 상에서 유도 방법에 따른 수직적 두부회전 정도의 차이를 비교하는 한편, 측모 및 정모두부방사선규격사진에서 시간 간격을 두고 동일 방법으로 유도하여 촬영한 두 사진 상의 동일 계측항목 간 차이를 비교함으로써 각 두부자세의 유도방법에 대한 재현성을 평가하였다. 연구결과 SBP법으로 유도한 자연두부자세는 HPA법에 의해 유도된 자연두부자세보다 평균 2.79˚ 고개를 숙인 자세로 나타났다. 또한 HPA법 또는 SBP법을 이용하여 시간 간격을 두고 각각 2회 촬영한 측모두부방사선규격사진과 정모두부방사선규격사진 상의 계측치는 모든 계측항목에서 통계적으로 유의한 차이를 보이지 않는 것으로 나타났다. 본 연구 결과는 HPA법과 SBP법으로 유도한 자연두부자세가 모두 재현성이 있음을 나타내는 것으로, 두부방사선규격사진 촬영 시 재현성을 높이기 위하여 HPA법과 더불어 SBP법도 임상적으로 사용될 수 있음을 시사하였다. The purpose of this study was to compare the degree of vertical head rotation and to evaluate the reproducibility of natural head posture (NHP) according to two guiding methods. The head posture aligner (HPA) method and the self balance posture (SBP) method. The subjects consisted of 30 adults. On the first day. Lateral and frontal cephalometric radiographs were obtained through the two guiding methods. One month later. lateral and frontal cephalometric radiographs were obtained again through both guiding methods. The degrees of vertical head rotation of both guiding methods were compared and the reproducibility was evaluated for each guiding method. A comparison of the degrees of vertical head rotation for the two methods revealed that the vertical head posture was lower in the SBP method than in the HPA method by an average of 2.79˚. All measurements obtained using the HPA and SBP methods with a time interval of one month did not show any significant difference in lateral and frontal cephalometric radiographs. The results of the present study suggest that the SBP method may be used as an alternative to the HPA method in case the HPA method can not be applied.

일부 농촌 지역의 취학전 어린이 시력에 관한 연구

송기철,이현,이효,안금순 대한보건협회 2003 대한보건연구 Vol.29 No.2

We performed ophthalmic examinations for 569 children in cheong yang(3-6 years of age), during May, 2002. 78 children were shown to have either visual acuity of less than 0.5 or anisometropia of over 2 lines. Detailed ophthalmic examinations were conducted on those 78 children. The 69 children(l2.1%) had ametropic eyes, 14(2.5%) amblyopia, and 3(0.5%) strabismus. Among the types of ametropia, Myopic astigmatism 21(30.4%) was the most common type. Astigmatism was combined with all other types in 56(81.2%) children. Among the types of amblyopia, visual deprivation amblyopia was 8(57.1%) and anisometropic amblyopia was 6(42.9%). In cases of strabismus, exotropia was 3. The early detection and adequate care of eye problems for preschool children are important to prevent the permanent visual disability. It is necessary to make institutional devices for preschool vision screening and participation rate for this screening will be more increased by efforts of constitutional support and parental cooperation.

Melatonin이 암세포주의 미세구조 및 세표바깥기질단백에 미치는 영향

성언기,정현규,김주영,1이융창,송인환 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.2

雄性不稔性 파의 花粉發育의 細胞學的 觀察 : Allium fistulosum L.

宋基現,金鎭漢 忠北大學校 農業科學硏究所 2002 農業科學硏究 Vol.19 No.-

In 1989, a new male sterile mutant (MS216) was found on the Breeding and Research Station of Hungnong, which is assumed ofr cytoplasmic male sterility. This experiment was performed to study on the histological differences between the CMS line derived from MS216 ans AS10 which is genic male sterile line. The results were summarized as follows. 1 In histological observation microspore degeneration of GMS line began around dyad stage. However, in anther of CMS line derived from MS216, it was observed to begin around tetrad stage. 2 In anther of GMS line, severe hypertrophy was observed in their tapetal layer from dyad stage and the trace of tapetum remamed until blooming stage In anther of CMS line, hypertrophy in their tapetal layer was also observed at tetrad stage. But, it disappeared prematurely after microspore stage. 3 In anther of CMS line, shrinking up of the anther locule was prevailing phenomena, but it was not observed in GMS or MF line.

외과적 보조의 급속상악확장술 : 치험 5예 REPORT OF 5 CASES

박충열,이용욱,송종운,김영운,박홍주,오희균,유선열,이기현,황현식 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.1

Five adults (3 cases of bilateral posterior crossbite, 2 cases of unilateral posterior crossbite) with intermaxillary discrepancy of over 5㎜ due to maxillary transverse deficiency were treated by surgically assisted rapid maxillary expansion (SARME). Bilateral maxillary horizontal osteotomies, osteotomies of pterygomaxillary junction and anterior lateral nasal wall, ans anterior palatal osteotomy were performed in 4 cases, and unilateral osteotomies in 1case, followed by palatal expansion with tooth-borne orthopedic applicance. No significant complication was observed. The mean palatal expansion was 7.2㎜ (5.8∼10.0㎜) in the maxillary first molar region. During the mean follow-up period of 4 years (5 months∼8 years 7 months), no recurrence of crossbite was observed in all cases. The SARME seemed to be a simple, stable, and reliable procedure for achieving a permanent increase of over 5㎜ in skeletal maxillary transverse width of adults.

위암 환자에서 세포성 면역 기능에 관한 연구

이재익,윤일국,이종선,김종완,장준,안정기,송민호,정현용,이헌영,김삼용,김영건 충남대학교 암연구소 1991 癌共同硏究所 硏究誌 Vol.1 No.1

We performed a variety of lymphocyte stimulation tests, delayed hypersensitivity skin tests, and enumeration of several lymphocyte subpopulations in 21 patients with gastric cancer who did not receive any form of anticancer therapy, and in 20 healthy controls. The gastric cancer patients had significantly decreased number and total score of positive reactions in delayed hypersensitivity skin tests compared with the healthy controls. The percent of CD_(4) positive lymphocytes in the gastric cancer patients was significantly decreased compared to the normal control subjects, but it had no correlation with the total score of delayed cutaneous hypersensitivity reactions. The ratio of helper to suppressor cells was lower in the cancer group. The uptake of 3H-thymidine was markedly depressed in cancer patients when stimulated with various mitogens. There was little correlation between any of the stimulation tests and any of the lymphocyte subpopulation proportions to delayed hypersensitivity cutaneous reactions. Optimal proliferative response was found in lymphocytes stimulated with 10 micrograms of concanavalin-A and 10 microgram of phytohemagglutinin. Advanced stage patients had greatly depressed delayed hypersensitivity skin reactions and proliferative responses to mitogens compared with localized diseases. These results suggest that gastric cancer patients have depressed cellular immune functions, which mainly result from the decreased helper cells and defects in functional proliferative response to mitogens. Interleukin-2 and interferon-gamma restored the in vitro proliferative response of lymphocyte in patients with gastric cancer.

위암 환자에게 세포성 면역 기능에 관한 연구

이재익,윤일국,이종선,김종완,장준,안정기,송민호,정현용,이헌영,김상용,김영건 忠南大學校 癌共同硏究所 1991 癌共同硏究所 硏究誌 Vol.1991 No.-

Silibinin Inhibits Tumor Growth through Downregulation of Extracellular Signal-Regulated Kinase and Akt <i>in Vitro</i> and <i>in Vivo</i> in Human Ovarian Cancer Cells

Cho, Hyun Jin,Suh, Dong Soo,Moon, Soo Hyeon,Song, Yong Jung,Yoon, Man Soo,Park, Do Yoon,Choi, Kyung Un,Kim, Yong Keun,Kim, Ki Hyung American Chemical Society 2013 Journal of agricultural and food chemistry Vol.61 No.17

<P>Anticancer activity of silibinin, a flavonoid, has been demonstrated in various cancer cell types. However, the underlying mechanisms were not elucidated in human ovarian cancer cells. The present study was undertaken to examine the effect of silibinin <I>in vitro</I> and <I>in vivo</I> on tumor growth in human ovarian cancer cells. Silibinin decreased cell viability in a dose- and time-dependent manner. Silibinin caused an increase in reactive oxygen species (ROS) generation, and the silibinin-induced cell death was prevented by the antioxidant <I>N</I>-acetylcysteine (NAC). Western blot analysis showed silibinin-induced downregulation of extracellular signal-regulated kinase (ERK) and Akt. Transfection of constitutively active forms of MEK and Akt prevented the silibinin-induced cell death. Oral administration of silibinin in animals with subcutaneous A2780 cells reduced tumor volume. Subsequent tumor tissue analysis showed that silibinin treatment induced a decrease in Ki-67-positive cells, an increase in transferase-mediated dUTP nick end labeling (TUNEL)-positive cells, activation of caspase-3, and inhibition of p-ERK and p-Akt. These results indicate that silibinin reduces tumor growth through inhibition of ERK and Akt in human ovarian cancer cells. These data suggest that silibinin may serve as a potential therapeutic agent for human ovarian cancers.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2013/jafcau.2013.61.issue-17/jf400192v/production/images/medium/jf-2013-00192v_0008.gif'></P>

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1997)

김재홍,문득곤,김정수,김용준,임동진,박상훈,김희성,이민수,송기훈,김갑형,김형석,성소영,이인섭,김석우,황지환,조창근,김경문,부태성 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.3

Background : In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. Objective & Methods : For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results : In 1997. 99 strains of N. gonorrhoeae were isolated, among which 45(45.5%) were PPNG. Conclusion : The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 45.5% in 1997.