경관영향평가 항목선정을 위한 타당성 평가

오명성,조현주,이현택,나정화 경북대학교 농업과학기술연구소 2008 慶北大農學誌 Vol.26 No.-

This research is significant in terms of estimating the validity by setting evaluation items in view of integrating not only original beauty and visual areas but also natural ecological areas based on questionnaire. The results are as follows. 1) According to literature study, 17 items such as variety, the character of sites in terms of landscape, the beauty of landscape, visibility, and ratio of green visibility are selected. Also, 21 items such as variety of animals and plants species, size of green area, and ecological naturalness are selected m the area of landscape ecological resources. 2) As a result of questionnaire of the group of landscape experts, animals and plants ecological areas show 5.6341, the highest in importance analysis according to assessment areas. Also, as a result of importance analysis on items in each area, for example, in the area of visual resources, the item of skyline analysis is 6.0488, the highest. 3) As a result of corelation of item meaning on landscape effect assesment, for example, meaningness of psychological assessment item and landscape site item indicate 0.710, the highest coefficient correlation. 4) As critical assessment items per unit project, items such as damage minimization of original land features for project in terms of point, ratio of green visibility, variety of animals and plants species marked above 8.0 as high important medium. The project in terms of line, minimization of original land features, Preservation of worthy biotope showed high point and the character of sites in terms of landscape, minimization of original land features, the size of green area, and analysis of skyline marked above 8.5 as high importance points. On the contrary, items for climate and soil showed relatively low points.

발치와의 치유과정에서 교원질 및 비교원단백질의 분포에 관한 면역조직화학적 연구

오화탁,김현수,주성숙,신제원 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

During tooth socket healing, the coagulum in the socket is replaced by fibrous connective tissue which undergoes mineralization and eventually becomes bone. Using this model, the healing process of the tooth socket and the role of type I collagen(CI), fibronectin(FN), bone sialoprotein(BSP), and osteopontin(OP) in the process were studied. Thirty Sprague-Dawley rats weighing 130-150gm were fed fi -aminopropionitcille for 5days before extraction of the first maxillary molars, and sacrified by perfusion with 4% paraformaldehyde at 1, 3, 5, 7, and 10days after tooth extraction. The socket and surrounding tissues were cut, demineralized, dehydrated and embedded in paraffin. For morphological observation, the sections were stained by Azan. Immunostaining of the ECM components was achieved by the avidin-biotin complex method. The results as follows; 1. Morphology of the tooth socket 1day after tooth extraction. The socket was filled with blood coagulum which was composed of densly aggregated red blood cells, platelets and fibrin network. 2. Morphology of the tooth socket 3days after tooth exyraction. A large portion of the coagulum was replaced by fibrous connective tissue contains a large number of fibroblasts, come from periphery of the socket. 3. At 5days, the newly formed bone demonstrated the highest proliferation activity. At 7days, the soclet was occupied with new bone. 4. Type I collagen was observed in the newly formed connective tissue and around the new bone. fiber. 5. Intensive staining for fibronectin was observed in granulation tissue, especially around blood vessels as well as inflammatory cells. 6. Strong immunostaining for bone sialoprotein and osteopontin was found in osteoblasts and new bone, while weaker staining was observed on dense connective tissue. 7. These data suggest that collagen and noncollagen proteins (FN, BSP, OP) an important role daring socket healing.

광주광역시 도시공간구조 변화에 관한 연구

오재화,김우혁,김현웅 朝鮮大學校 建設技術硏究所 2002 建設技術硏究 Vol.22 No.1

The purpose of this thesis is to inspect the step of spatial structure varied with the urban growth in place of Kwang-ju, to analyze the developing step of urban growth on the basis of the variety of shape-index and function-index on urban from 1930 to 1992 each year, to predict the variety direction of urban spatial structure for the concentrating and efficient urban function and to grope for the prospects of urban : what is the municipal concept in the land use, the variety of population, the change of traffic system, the change of urban planning zone, the change of executive zone, the transition of spatial structure on the basis of urban growth in Kwangiu. Considering the advent of subway in 1990, Pyoung-Dong industrial block, to work out these factors, the urban pattern in Kwangiu would be developed and established in aspect of long-range planning. The size of population, the changing pattern of land use planning and zonning are compared and analyzed to justify the relation with the urban comprehensive planning circulation planning.

결핵균 30-kDa와 32-kDa 단백항원의 분리정제와 마우스 비장세포 증식에 미치는 영향

백태현,오명주,김화중,조은경,박정규,최대경 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.1

Identification and characterization of individual components of M.tuberculosis have long been a focus of research on tuberculosis. The 30-kDa and 32-kDa antigens are major constituents of M bovis BCG and M. tuberculosis culture fluids. Because 30-kDa and 32-kDa antigens are partially identical, these antigens are difficult to purify in large amounts by biochemical techniques. This study was performed to purify the 30-kDa and 32-kDa antigens complexes primarily purified by 50% ammonium sulfate precipitation, hydroxylapatite chromatography and Sephadex G-75 gel filtration. And then further purification for separation of the two antigens was accomplished on preparative isoelectric focusing. Recovery of 30-kDa and 32-kDa antigens during above the purification procedures were 28% and 14%, respectively, and 147.0 and 59.8-fold purification were showed, respectively. On silver stained SDS-PAGE gels, the purified 32-kDa antigen gave a single band at 32-kDa molecule, while 30-kDa antigen gave one major band at 30-kDa molecule and faint additional band at 32-kDa. The pI of 30kDa-and 32-kDa antigens were 4.3 and 4.6, respectively. The partial identity between these two antigens was observed through the same pattern of reactivity of antigens in the ELISA and precipitation pattern of immunodiffusion. We also examined the immunological activities of both purified antigens by splenocyte proliferation of BCG-infected mice. Proliferative response to the 30-kDa and 32-kDa antigens did not show significant difference. However, macrophage-like cells existed in spleen cells from BCG-infected mice were involved in the suppression of the proliferative response to mycobacterial antigen. These results suggest that the 30-kDa and 32-kDa antigens could be effectively purified by the IEF and reactions of partial identity between the two antigens were found. However, 30-kDa antigen was the more immunogenic antigen than 32-kilodalton.

남제주 화력발전소의 증설과 저감대대책의 영향 분석

이화운,김유근,정우식,오은주,임헌호,노순아,반수진,최현정,김민선,김헌숙 부산대학교 환경문제연구소 2002 環境硏究報 Vol.20 No.-

In the view of the results investigated the effect of air pollutants that is emitted from a new generator facilities, the total estimated concentration which is the summation of a contributional concentration and observational concentration is much less than that of atmospheric environment criterion for the air pollutants. So we can predict that the operation of the new generator facilities with mitigation equipment don't seriously affect around environment. At the same time, we compared the contributional concentration of before mitigation equipment with that of after it to examine the decreasing amount due to mitigation equipment. The result showed that the concentration of SO2, NO2 and PM-10 is reduced to 25%, 30% and 26% of original value, respectively.

일회통과 관류실험시 물의 수송 : 관류액의 종류와 삼투압의 영향 Effects of Some Perfusates and Their Osmolality

이정화,이현주,용철순,오두만 영남대학교 약품개발연구소 1995 영남대학교 약품개발연구소 연구업적집 Vol.5 No.-

The single pass perfusion experiments were performed in anesthetized rata to investigate the effects of perfusates and their osmolality on the water transport and to determine the correlation between the extent of water transport and the volume change of perfusate. Phenol red was used as a nonabsorbable marker. In normal rats, when perfused at a flow rate of 0.5 ml/min 2-(N-morpholino) ethanesulfonic acid (MES) aid Sorensen's phosphate hullers showed minimal net water transport as 0.125 and 0.173 %/cm of intestinal length, respecetively. Hypotonic perfusate of 200 mOsm㎏ of water and hypertonic perfusate of 400 mOsm/㎏ of water generated significant water transport compared with isotonic perfusate of 300 mOsn/㎏ of water. There was a linear correlation between the attent of water transport and the volume change of perfusate, suggesting that the volume change can be used as a measure of water transport.

효율적인 영상 이진화를 위한 적응적 Water Flow Model에 관한 연구

임길택,오현화 경주대학교 정보전자기술연구소 2004 情報電子技術論叢 Vol.3 No.-

In this paper, we propose an improved method to overcome the drawbacks of the existing water flow model based method for document image binarization. The proposed method defines the region of interest (ROI) around character areas to restrict rainfall onto the terrain surface. The amount of water required to fill a local valley is automatically determined according to the properties of the valley, thereby eliminating an iterative rainfall procedure which is very time consuming process in the existing method. Finally, to threshold the amount of water filled, a local adaptive method is applied to each pond. In the experiments on three document images, the proposed method showed better performances than the existing water flow model based method in terms of both quality and speed.

Vibrio vulnificus에 의한 패혈증 3예

김신무,김유현,신상희,오재세,강숙희,윤여안,이화재,김영옥 대한임상병리사협회 1986 임상병리사회지 Vol.18 No.1

관상동맥 질환에서 CD14 유전자형에 따른 세균 및 Heat Shock Protein에 대한 반응의 차이

한주용,최수연,조현주,김화평,강현재,구본권,김남중,김효수,손대원,오병희,박영배,최윤식 대한감염학회 2007 감염과 화학요법 Vol.39 No.1

Background : CD14 is the receptor for lipopolysaccharides and heat shock protein (HSP), which has been suggested being associated with increased risk of coronary artery disease (CAD). We investigated whether the response to infectious agents or HSP is different according to CD14 polymorphism in Koreans. Materials and Methods : Antibody titers to Helicobacter pylori, Chlamydia pneumoniae, and human HSP60 (hHSP60) were measured in 48 patients with stable CAD and in 41 healthy controls by ELISA. CD14 genotype was determined by PCR and high-sensitivity C-reactive protein (hs-CRP) was measured. Results : Seropositivity to C. pneumoniae and H. pylori, and antibody titer to hHSP60 were not significantly associated with the presence of CAD. CD14 genotype distribution was 31 TT (35%), 43 CT (48%), and 15 CC (17%). To compare the response to the infectious organism and hHSP60, we divided study population into 3 groups; CAD patients with non-TT genotype (group I, n=30), CAD patients with TT genotype (group II, n=18), and normal controls (group III, n=41). Seropositivity to C. pneumoniae and H. pylori, and antibody titer to hHSP60 were not significantly different among 3 groups. Though hs-CRP level was significantly different among 3 groups, post-Hoc analysis showed that hs-CRP level was not significantly different between group I and group II (group I: 1.6[1.1-3.5] mg/L and group II: 0.35[0.1-2.0] mg/L). Conclusions : This study suggests that the inflammatory responses to infectious organisms and HSP do not differ according to the CD14 genotype in Koreans. 목적 : 만성적인 세균 감염이나 자가 면역 반응이 동맥경화와 연관되어 있다는 주장이 제기되어 왔다. CD14은 lipopolysaccharides (LPS)와 heat shock protein(HSP)의 수용체로 C(-260)→T 다형성이 관상동맥 질환의 위험과 관련되어 있다고 제안되어 왔다. 본 연구에서는 한국인에서 CD14 다형성에 따른 LPS와 HSP에 대한 반응성을 염증표지자를 측정하여 살펴보고자 하였다. 재료 및 방법 : 안정형 관상동맥 환자 48명과 정상 대조군 41명을 대상으로 Chlamydia pneumoniae, Helicobacter pylori 항체 및 인체 HSP60 (hHSP60) 항체 역가를 enzyme-linked immunosorbent assay (ELISA) 방법으로 측정하였다. CD14 유전자형은 중합효소연쇄반응을 이용하여 결정하였고 hs-CRP를 측정하였다. 결과 : C. pneumonias 및 H. pylori 항체 양성 여부 및 항체 역가, 그리고 hHSP60에 대한 항체 역가는 안정형 관상동맥 질환의 유무와 유의한 상관관계는 없었다. CD14 유전자형의 분포는 TT 30명(39%), CT 31명(40%), 그리고 CC 16명(21%)이었다. 관상동맥 질환 군에서 TT 유전자형은 38%, 대조군에서는 32%로 TT 유전자형과 관상동맥 질환의 유무와는 유의한 상관관계가 발견되지 않았다(P>0.05). CD14 유전자형에 따라 C. pneumonias와 H.pylori 감염 및 hHSP60에 따른 반응에 차이가 있는지 여 부를 알아보기 위해, 관상동맥 질환 군을 다시 TT 유전자형 군과 non-TT 유전자형 군으로 나누어, 전체적으로 세군에서 항체 양성률과 역가를 비교하였다(I군: CD14 TT 유전자형을 가진 안정형 관상동맥 환자, II군: CD14 TT 유전자형을 가진 안정형 관상동맥 환자, III군: 정상 대조군). 세 군에서 C. pneumonias와 H. pylori 항체 양성률 및 hHSP60 항체 역가에는 유의한 차이가 없었다. 세 군사이에 hs-CRP 값에 유의한 차이가 있었지만, post-Hoc분석에서 II군의 hs-CRP 값이 I군에 비해 유의하게 높지는 않았다(0.35 [0.1-2.0] mg/L in group II vs. 1.6 [1.1-3.5]mg/L in group I, P>0.05). 결론 : 본 연구는 한국인에서 CD14 유전자형에 따라 LPS나 HSP에 대한 반응이 유의하게 다르지 않음을 시사한다.

면역적격 환자에서 발생한 폐 크립토코쿠스증 5예

오현정,최민영,김수현,송은미,백두현,김지혜,류연주,천은미,이진화,장중현,심성신 이화여자대학교 의과학연구소 2010 EMJ (Ewha medical journal) Vol.33 No.2

폐 크립토코쿠스증은 최근 스테로이드 제제를 포함한 면역억제제의 사용으로 그 발생이 증가하고 있으며, 면역적격 환자들에게서 발생할 수 있다. 일부에서는 뇌수막염이나 패혈증과 같은 치명적인 전신감염을 초래할 수 있어, 이에 대한 진단 및 적절한 치료가 중요하다. 본 증례에서는 면역저하의 증거가 없는 환자들에서 다양한 폐질환의 양상으로 발병한 폐 크립토코쿠스증 5예를 경험하여 문헌고찰과 함께 보고하는 바이다. Cryptococcal infections occur frequently in patients with cell-mediated immune deficiency, such as acquired immune deficiency syndrome, organ transplants, hematologic malignancy patients and immunocompromised patients who receiving cancer chemotherapy or corticosteroid therapy. In these conditions, cryptococcosis can cause serious infections requiring systemic antifungal therapy such as meningoencephalitis and sepsis. However, pulmonary cryptococcosis in immunocompetent hosts may have self-limited course and improve without treatment. We experienced various pulmonary manifestations of pulmonary cryptococcosis in five immunocompetent patients. We diagnosed three cases of isolated pulmonary cryptococcosis and two cases of disseminated cryptococcosis involving lungs. All cases were treated with intravenous injection of amphotericin B during hostpital care, and maintained with oral fluconazole after discharge. During the follow-up, clinical symptoms and radiologic features were markedly improved.