백서 치은암의 골침투성 증식에 관한 실험적 연구

송요섭,조한국 慶熙大學校 齒科大學 1989 慶熙齒大論文集 Vol.11 No.-

The purpose of this study was to reach a better understanding of the mechanism of bone invasion by DMBA (9, 10-dimethyl[-1, 2-benzanthracene) induced carcinoma of the gingiva in the white rat. Fourty seven white rats were divided into the experimental and control group. In the experimental group gingiva was incisioned and about 5mg pellet of DMBA powder was implanted into the incisioned gingiva. After implantation the incisioned gingiva was sutured. Each 7 animals in the experimental group and 1 animal in the control group were sacrificed at 4, 6, 8, 10, 12 and 14 weeks after start of experiment. At the time of sacrificed, the mandible were removed and specimens were fixed with 10% neutral formalin solution, and embedded in paraffin as usual manner. Embedded specimens were sectioned in 4-6p with microtome, stained with Hematoxylineosin and observed through the ordinary lightmicroscope. The results were as follows; 1 In all specimens, severe coagulative necrosis and connective proliferation were observed at the site of DMBA implantation. As a severe inflammatory reaction, fascicular degeneration or phagocytic reaction of alveolar bone was observed at the adjacent area. 2 In 6 weeks, basal cells hyperplasia of the gingival epithelium revealed carcinoma in situ, and the inflammatory reaction in the lamina propria and periodontal tissue especially lymphocyte and plasma cell infiltration were prominent, and enlarged Haversian canal and connective proliferation into enlarged Volkman canal were observed. 3 In 8 weeks, well differentiated squamous cell carcinoma was derived from the gingival epithelium and Malassez epithelium, and in 10 weeks, the squamous cell carcinoma was proliferated into the enlarged Volkman canal. 4. In 12-14 weeks, well differentiated squamous cell carcinoma was proliferated into enlarged Volkman canal deeply, but proliferation into the Haversian canal and bone marrow were not observed. 5 In the opposite site of implantation, carcinoma in situ was observed in the labial gingival epithelium from 10 weeks,'and prominent proliferation was observed in 12-14 weeks.

치은섬유모세포 및 치주인대세포에 의한 교원질 수축에 관한 연구

홍영안,김현수,주성숙,신제원 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

The purpose of this in vitro study was to evaluate contraction of the human gingival and periodontal ligament fibroblast in the collagen matrix. The cell cultures were prepared using human gingiva and tooth. The collagen gels were observed daily with an inverted microscope and their area was determined by measuring the diameters of the collagen gel. The configurations of human gingival and periodontal ligament fibroblasts were done by SEM observation. The results as follows; 1. The contraction was found to be proportional to seeding cell without seeding cells, the contraction was not occured. 2. Periodontal ligament fibroblast was shown more fast contraction than gingival fibroblast. 3. The collagen matrix was translucent initially, but with fully contraction, the structure become opaque. 4. The collagen matrix with fibroblast were aggregated to form bundles and reorganized collagen fiber.

알코올과 스트레스가 Cyrochrome P450 발현에 미치는 영향에 관한 면역학적 연구

전양현,홍정표 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

전치 유도각의 인위적 증가에 의한 저작근과 하악 운동 양상의 변화에 대한 연구

이용식,최대균,최부병 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

This study' was performed to measure the changes of the mandibular movement and the masticatory muscular activities anterior temporal and masseter muscle of both side - reflected by intentional increase of anterior guidance angle. For this study, 5 volunteers (3 males and 2 females with average age of 24.0) were selected. Each volunteer had Angle's classification I and did not have any missing tooth except third molar and any extensive restorations. Metallic guide plate was made at volunteer's working model fabricated by improved dental stone and cemented to the palatal surface of maxillary central incisor using resin cement(Panavia 21�) and then adjusted not to give any occlusal interferences at intercuspal position. The activity of masticatory muscles and the changes of mandibular movement were recorded by EMG and Sirognathograph in Biopak analysing system(Bioresearch Inc., Milwaukee, Wisconsin, USA). Measurement was done at before experiment, irnmediatley after placement, 1 week after placement, immediately after removal, and 1 week after removal. The results were as follows: 1. Moderate phonetic disturbance and mild headache were occured to 3 volunteers for 2 days after setting and l volunteer had positive reaction to percussion and slight midline diastema. But all of these clinical signs were diappeared 1 week after removal and the other volunteer did not have any special clinical sign. 2. In the EMG of the mandibular rest position, the mean value of anterior tempotal muscle was increased immediately after placement(p<0.01) and then decreased 1 week after placement(p<0.05) and increased 1 week after removal(p<0.05) but not recovered as before experiment. The mean value of masseter muscle was decreased during the experiment period: 3. In the EMG during mandibular protrusive movement, all muscular activity was decreased during the experiment period. Reduced activity was not recovered I week after? removal(p<0.05). 4. During the habitual opening, anteroposterior movement of mandible was decreased immediately after place ment(p<0.05) and then increased 1 week after placement but not statistically significant(p>0.1). Vertical movement was not shown significant difference during the experiment period(p>0.1). Lateral movement was decreased immediately after placement(p<:0.05) and then increased 1 week after placement but not recovered as before experiment. The opening and closing velocity of mandible was shown minor changes but not statistically significant. 5. During the habitual opening, anteroposterior movement of mandible was decreased 1 week after placement(p<0.(6) and then increased immediately after removal and recovered 1 week after removal as before experiment. Vertical movement was not shown significant changes. Lateral displacement of mandible was increased continuously and recovered 1 week after removal. Opening velocity was temporarily increased immediately after removal but recovered and closing velocity was not shown significant changes. 6. During the right side chewing, anteroposterior movement of mandible was increased immediately after removal but recovered and vertical movement was not shown statistically significant results. Lateral displacement and velocity o mandible were not shown significant results. 7. During the left side chewing, the changes of mandibular movement pattern were not shown statistically significant results.

레진접착제를 도포한 상아질에 대한 합착용 시멘트의 전단결합강도

김교철,우이형,이성복,최부병 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

The purpose of this study was to confirm the formation of hybrid layer and resin tags in dentin tissue and the possibility of bonding between luting cements used for the prosthesis and the resinous surface coated with resin bonding agents to prevent the dentin hypersensitivity after abutment preparation. Some resin bonding agents, which may have the possibility of bonding with polyacrylic acid as a liquid ingredient of polycarboxylate and glass ionomer cements, were selected. All-Bond desensitizer containing NTG-GMA and BPDM, Scotch-Bond Multipurpose plus containing HEMA, and XR-bond containing organophosphate were selected as a coating agent. Dental cements were zinc phosphate, polycarboxylate, and glass ionomer cement. After the exposed dentin surface of premolars was ethced with 10% phosphoric acid and coated with resin bonding agents, the morphology of treated surfaces and the resin tags and hybrid layers on sectioned surfaces were observed by SEM. Shear bond strength between the resin bonding agents and 3 kinds of cements was measured 24 hours after bonding. On the debonded surfaces of the shear bond strength tested specimens, the cement tags and the bonding sites between the resin materials and cements were examined by SEM. Following conclusions were drawn 1. Coating of dentin with resin bonding agents had no effect on the shear bond strength of zinc phosphate cement. 2. Both of polycarboxylate and glass ionomer cements showed the increased shear bond strength by the dentinal coating with Scotch-Bond Multipurpose plus containing HEMA. However, in the case of dentinal coating with some agents containing NTG-GMA and BPDM or organophosphate, polycarboxylate cement exhibited the lowered shear bond strength, and glass ionomer cement showed the unchanged shear bond strength. 3. Complete obstructions of dentinal tubules were observed on the dentin coated with All-Bond desensitizer or XR-bond, but distinct shape of the orifices of dentinal tubules was observed consistently on the dentin coated with Scotch-Bond Multipurpose plus. 4. The hybrid layer was thickest on the dentin coated with Al1'Bond desensitizer, and the length of resin tags was longest on the dentin coated with Scotch-Bond Multipurpose plus. 5. On the debonded specimens which had been bonded with polycarboxylate cement or glass ionomer cement after coating with Scotch-Bond Multipurpose plus, the cement tags and the bonding sites between the resinous surface and the cements could be examined.

형질변형성장인자가 손상골 재생능에 미치는 영향에 과한 실험적 연구

이병도,황의환,이상래,홍정표 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

The purpose of this study was to observe the effect of TGF-81, which promotes differentiation and proliferation of osteoblasts, on bone regeneration. Experimental bone defects that measured 3 mm in diameter were created on the mandibles of guinea pig by removal of bone with the use of trephine burs. In one side of mandibular body, the experimental groups, bone defects were grafted with Biogran(Orthovita Co., U.S.A.) and TGF 131(R&D System Co., U.S.A.). In the remaining side of the mandiblar body, the control groups, bone defects were grafted with only Biogran. Guinea pigs in the control and experimental groups were serially terminated by fours on the 3 days, the 1 week, the 2 weeks, the 3 weeks, and the 4 weeks after experiment, and both sides of the mandibular bodies were removed and fixed with 10% neutral formalin. They were decalcified and embedded in paraffin as using the usual method. The specimen sectioned and stained with hematoxylin and eosin. Also, they were radiographed with a soft X-ray apparatus. The Obtained results.=were as follows; 1. Hemorrhagic condition, observed in the granulation tissues, disappeared on the 1 week after experiment in both groups, and more prominent in the experimental group. The granulation tissues of the experimental group had larger number of cells than those of the control group. 2. Osteoblastic differentiation in the margin of grafted material and adjacent bone was observed on the 1 week after experiment in both groups. Also, bone formation was observed in immature form on the 1 week after experiment, and more prominent in the experimental group. 3. In the polarizing microscopic examination, bone matrix was very loose on the 1 week after experiment, but increase in density with time, and more prominent in the experimental group. 4. In the microradiographic examination, newly formed bone was observed in the experimental group on the 2 weeks after experiment, and this was observed earlier than in the control group. Newly formed bone was increased with time and defected area was markedly decreased on the 4 weeks after experiment.

한국 성인 유치악자의 하악 치열궁에 관한 조사

김일한,박남수,최대균 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

The purposes of this study are to evaluate the Korean mandibular dental arch and classify the mandibular dental arch shape and size based on the incisal angle, canine angle, inter second molar width and height. In this study the mandibular study models were fabricated using irreversible hydrocolloid impression material from 225 volunteers with a mean age 23.62 (range 19-29). And the study models were measured with 3-dimensional measuring device and the mandibular dental arch was classified by means of K-means clustering method and visual inspection, then obtained data were analyzed with t-test for the statistical analysis. The results `were as follows ; 1. The average canine height was 5.19mm(s.d. 1.17) in both sex, 5.34mm in male, and 35.83mm in female. And the sexual difference was significant(0<p <―0.05). 2. The average second molar height was 39.81mm(sd. 2.44) in both sex, 40.19mm in male, and 39.21mm in female. And the sexual difference was significant(0<p50.01). 3. The average inter-canine width was 27.16mm(s.d. 1.78) in both sex, 27.41mm in male, and 26.77mm in female. And the sexual difference was significant(0<p<0.01). 4. The average inter-first molar width was 46.93mm(s.d. 2.67) in both sex, 47.72mm in male, and 45.7mm in female And the sexual difference was significant(0<p―<0.01). 5. 'the inter-second molar width was average 56.09mm(s.d. 3.01) in both sex, 57.24mm in male, and 54.32mm in woma. And the sexual difference was significant(0<p<―0.01). 6. The arch form was classified into three shapes based on the incisal and canine angle. V-shape showed 124.88 of incisal angle and 141.64 of canine angle, U-shape showed 152.76° and 125.35° , and 0-shape showed 138:03° and 133.66° respectively. Each arch shape distribution was that the V-shape was 142%, the Ushape was 14.7%, and the 0-shape was 71.1% of the 225 study models. 7. It was thought that the use of second molar width is more reasonable than height for classifying the dental arch size. The arch size was classified into four sizes based on the 2nd molar width. Size 1 showed range of 42.24-48.'23 mm, size 2 showed 48.24-54.23mm, and size 3 showed 54.24-60.23mm size 4 showed 60.24-66.23mm respectively Each arch size distribution was that the size I was 1.3%, the size 2 was 27.1%, the size 3 was 63.6%, and the size 4 was 8:0% of the 225 study models.

악안면부에 대한 수종 온냉요법시의 체열변화에 관한 비교연구

김선호,홍정표,황의환 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

The purpose of this study was t.o assess the efficacy of several thermal therapies using ice pack, moist-hot pack and ultrasound, separately and concomitantly and to obtain the background information on the vascular changes after thermophysical therapies. The author had used 15 healthy subjects were examined and the subjects were divided into 5 groups; ice pack, moist-hot pack, ultrasound, ice pack and moist-hot pack, ice pack and ultrasound. Observation were made immediate before and 0, 5, 10, 20, 30, 45, 60, 90 minutes after treatment. Thermography was performed in an Agema 870 thermovision with 0.1°C difference of gradual temperature shift, The results were as 'follows 1. Using ice pack only, the surface temperature of the masseter region was increased lapse of time, and most remarkably 90 minutes after the treatment. 2. Using moist-hot pack only, the surface temperature of the region was remarkably increased immediately after the treatment, but decreased lapse of time. 3. Using moist-hot pack with ice pack, the surface temperature of the face was remarkably increased immediately after the treatment, and decreased lapse of time. Hyperthermia was maintained for a longer time as compared with the group of moist-hot pack only. 4. Using ultrasound only, the surface temperature of the region was increased gradually, and most remarkably 30 minutes after the treatment, but decreased in the course of time. 5. Using ultrasound combined with ice pack, the surface temperature of the region was gradually decreased until 30 minutes after the treatment, and decrease to some extend at 45 minute And then a gradual increase observed over the remaining period of the experiment. 6. Hyperthermia were maintained for a longer time in the groups using ice pack combined with moist-hot pack and ultrasound as compared with the other groups. Our data suggest that ice pack can promote the efficacy of other thermal therapies.

Riluzole의 칼슘전류 억제효과에 관한 연구

문철현,김형찬 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

Riluzole is a recently synthesized antiglutarnate drug which has the potential therapeutic effect in amyotrophic lateral sclerosis(ALS). The aims of this experiment are to elucidate the pharmacological effect of riluzole on the calcium channels in bovine adrenal chromaffin cells and to verify the mechanism of pathogenesis of ALS, The authors recorded and analyzed the calcium currents of bovine adrenal chromaffin cells in the presence of riluzole by using whole-cell patch clamp technique. The results are as follows. 1. ICa.max was recorded at a voltage of 10 mV. 2. Riluzole inhibited ICaPK & ICaSS of bovine adrenal chromaffin cells. 3. There was a dose-dependent manner in the inhibiting rate of riluzole tested on calcium currents. 4. Inhibitory effect of riluzole on the ICaSS was greater than that on the ICaPK. Therapeutic effect of riluzole in amyotrophic lateral sclerosis seems to be related with the mobilization of calcium ions through voltage-gated calcium channels.

흰쥐 대구치의 치수강 노출 후 치유 및 형성과정에서 치수와 상아질 기질내의 교원질과 당단백의 분포에 관한 면역조직화학적 연구

김병우,민병순,최호영,박상진,최기운 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

The development and repair requires the formation of new tissues comprised of various extracellular matrix components. The present study investigated the formation and distribution of the major ECM components such as type I collagen, type III collagen, fibronectin, bone sialoprotein, and osteonectin during development and repair. For developing observation, Sprague-Dawley rats weighing 27±1gm were sacrificed. For repair ohsei'ation, Sprague-Dawley rats weighing 110±5 gm were used. The pulp perforation were prepared on mesial surface of the maxillary first molar by using 1/2 round bur. At 5 days after perforation, rats were sacrificed by perfusion with 3% paraformaldehyde. The maxillary first molar region were cut, demineralized, dehydrated and embedded in paraffin. Immunostaining of the ECM components was achieved by the avidin-biotin complex method. The results as follows ; 1. Bright immunoreaction for fibronectin was present in the basement membrane at the inner epithelial-mesenchymai interface, especially concentrated in the blood vessel walls,?ell membrane of odontoblasts, and initi it predentin: 2. Type I and III collagen was observed in the newly fowled pulp tissue, predentin, and its intensity increased as more of these components during repair. 3. Strong immunostaining for bone sialoprotein and osteonectin was found in dentin while no or weaker staining was observed loose connective tissue of the pulp. 4. These results suggest that development and repair is achieved through a series of cell differentiation and attachment by the specific ECM components.