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장관세포인 HT-29 에 존재하는 디펩티드수송체의 Xenopus oocyte 에서의 발현
오두만,양재하 한국약제학회 1995 Journal of Pharmaceutical Investigation Vol.25 No.4
Cloning the gene encoding a dipeptide transporter is necessary for understanding the absorption mechanism of peptides and peptide-like drugs in the gastrointestinal tract. Functional expression of a dipeptide transporter after microinjection into Xenopus laevis oocytes was performed using the mRNA purified from human intestinal HT-29 cells. Fifty nanoliters of purified mRNA (1 ㎎/mL) were microinjected into healthy oocytes followed by incubation for 4 days in order to express a dipeptide transporter. Functional expression was determined by a uptake assay using 10Ci/mL [³H]-glycylsarcosine, a dipeptide substate of the transporter. Seasonal variability and batch-to-batch variability were greater in summer. The usage of beveled micropipettes improves viability of oocytes at 4 days after microinjection. Expression of a dipeptide transporter in oocytes after microinjection of mRNA obtained from HT-29 cells was significantly larger than those after microinjection of water or mRNA obtained from the rabbit intestine.
흰쥐의 소장에서 음식물이 암피실린의 흡수에 미치는 영향
오두만,이현주,이정화,권용준,양재하 한국약제학회 1995 Journal of Pharmaceutical Investigation Vol.25 No.4
The purpose of this study is to verify the interaction between food and ampicillin which is one of the aminopenicillins known to be absorbed by a specified dipeptide transporter in the small intestine. The absorption of ampicillin was measured in the presence of the high carbohydrate food, high fat food, and high protein food, and compared with that in the presence of the control normal food. In situ single-pass perfusion method was chosen in these experiments using two jejunal segments in the rat. Reduction in the absorption of ampicillin was not shown, when both high carbohydrate food and high fat food were co-perfused with ampicillin. When the high protein food was co-perfused with ampicillin, the difference of C_(out)/C_(in) of ampicillin ratio was 0.084 ± 0.082. showing a trend of reduced absorption without a significance. Further, glyclysarcosine (Gly-Sar) which is a stable dipeptide in the small intestine was used in order to see the direct competitive inhibition with ampicillin on the dipeptide transporter. The difference of C_(out)/C_(in) ratio was 0.078 ± 0.020 in the presence of 10 mM Gly-Sar, showing a significant inhibition of ampicillin absorption (p<0.02). It suggests that dietary di- and tripeptides, the digestive products of protein food, might have influence on the absorption of ampicilln, and that ampicillin could be given at the fasting state for better absorption.
HT-29 장관세포에 있는 디펩티드수송체에 의한 디펩티드의 흡수
오두만 한국약제학회 1995 Journal of Pharmaceutical Investigation Vol.25 No.2
The peptide transporter can be utilized for improving the bioavailability of compounds that are poorly absorbed. Characterization of the dipeptide uptake into the human intestinal epithelial cells, HT-29 was investigated. The uptake of tritiated glycylsarcosine ([³H]-Gly-Sar. 0.l μCi/㎖) was measured in confluent or subconfluent HT-29. Caco-2, and Cos-7 cells. Uptake medium was the Dulbecco's Modified Eagle's Media (DMEM) adjusted to pH 6.0. Both HT-29 and Caco-2 cells expressed the dipeptide transporter significantly (p<0.005) but Cos-7 did not. Certain portions of passive uptake were observed in all three cell lines. Uptake of Gly-Sar was largest at 7 days after plating HT-29 cells with significant inhibition with 25 mM cold Gly-Sar (p<0.05), but expression ratio of the dipeptide transporter was 0.7, suggesting lower expression. The effect of pH on Gly-Sar uptake was not significant in the range of pH 6 to 8. Gly-Sar uptake was also inhibited with 50 mM carnosine. 25 mM Gly-Sar, and 35 mM cephalexin significantly (p<0.05). From above results the dipeptide transporter was expressed well in HT-29 cells and was similar to that in the small intestine, suggesting that large amounts of mRNA of the transporter from the cells can be obtained.
감마선을 이용한 케나프 펄프 표면의 Poly(ethylene glycol) Methacrylate 그라프트 중합반응
오두리,전준표,강필현 한국방사선산업학회 2012 방사선산업학회지 Vol.6 No.3
Pulp is typically used for paper industry to manufacturing various types of papers. Howeversimply chemical modification makes enable the pulp to a wide range of application in variousindustrial fields. To bring the polymerization the gamma ray irradiated on the mixture of kenafand PEGMA in various dose ranges from 20 to 60 kGy. As a results, the graft degree of 20.0% wasobtained from 475 g of gamma ray irradiated pulp and PEGMA. After the polymerization, the chemicalstructure and morphology of the surfaces were examined by Fourier transform infrared spectroscopy,thermogravimetric analysis, and scanning electron microscope. Chemical structure ofgrafted pulp has significantly growth in carbonyl content with increasing the radiation dose. Alsosurface morphology was distinctly changed with decreased the degree of roughness and increasingthe diameter. These results were explained gamma ray irradiation improve performance of graftpolymerization efficiency.
오두리,전준표,신혜경,강필현 한국방사선산업학회 2012 방사선산업학회지 Vol.6 No.2
Kenaf (Hibiscuc cannabinus L.) is a renewable resource for industry and kenaf ligninis syringyl-guaiacyl lignin. The electron beam irradiated on kenaf core various doses range from200 to 1,000 kGy to improve delignification. The yield of lignin, which is 2.53 g from 10 g of electronbeam irradiated kenaf core. A comparison extracted lignin between from native kenaf core andelectron beam irradiated kenaf core was then studied through chemical structure and bondingproperty by a Fourier transform infrared spectroscopy (FT-IR). Thermal stability of the extractedpurifiedlignin was performed via differential scanning calorimetry (DSC). These results wereexplained that electron beam irradiation increased performance of extracting efficiency.