Genome-wide investigation of a Korean synthetic breed, Woori-Heukdon using the Illumina PorcineSNP60K BeadChip

Yong‑Min Kim,Ha‑Seung Seong,Jung‑Jae Lee,Da‑Hye Son,Jin‑Su Kim,Soo‑Jin Sa,Young‑Sin Kim,Tae‑Jeong Choi,Kyu‑Ho Cho,Joon‑Ki Hong,Jung‑Woo Choi,Eun‑Seok Cho 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Woori-Heukdon (KWH) is a Korean synthetic pig breed generated using Chookjin-Duroc (KCD), Chookjin-Chamdon (KCC), and their crossbreds. Currently, there is a severe lack of studies investigating the Korean breed populations including wild boars (KWB) throughout the genome. Objective: This study was performed to investigate the genetic characteristics of Korean pig populations at the genome-wide level. Methods: Using the SNP dataset derived from genotyped and downloaded datasets using the Illumina PorcineSNP60K BeadChip, we compared the genomes of 532 individuals derived from 23 pig breeds to assess the genetic diversity, inbreeding coefficient, genetic differentiation, and population structure. Results: KWB showed the lowest average expected heterozygosity (HE = 0.1904), while KWH showed the highest genetic diversity (HE = 0.02859) among Korean populations. We verified that the genetic composition of KWH, showing USD of 74.8% and KCC of 25.2% in ADMIXTURE analysis. In population structure analyses, KCC was consistently shown to be separated from other pig populations. In addition, we observed gene flow from Western pigs to a part of Chinese populations. Conclusion: This study showed that Korean native pigs, KCC have genetic differences in comparison with Chinese and Western pigs; despite some historical records and recent genetic studies, we could not find any clear evidence that KCC was significantly influenced by Chinese or Western breeds in this study. We also verified the theoretical genomic composition of KWH at the molecular level in structure analyses. To our knowledge, this is the first genomic study to investigate the genomic characteristics of KWH and KCC.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

응급실에 내원한 환자에서 어지럼증의 원인과 임상양상의 분석

문원식,정경운,위준선,문정미,전병조,김용권,소정일,류진호,허탁,민용일 대한응급의학회 2001 대한응급의학회지 Vol.12 No.3

Background: Recently, The number of patients who have been complaining of the vertigo or dizziness has been increasing due to rapid growth of elderly population and senile disease. The differentiation of dizzy patients is not familiar to most emergency physicians. This study was designed to differentiate true vertigo and to investigate the clinical difference among central vertigo, peripheral vertigo, and other causes of dizziness. Methods: The authors analyzed the cases of 237 dizzy patients who visited the emergency department of Chonnam University Hospital during the recent 2 years. For the base of dizziness, associated past illnesses, severity, nystagmus type, and the causes of central and peripheral vertigo. Results: Female patients were 142(59.9%) and male patients were 95(40.1%). The most common age group was the 6th decade. The origin of dizziness was classified as peripheral cause(25.3%), central cause(32.9%), and others(41.8%). As to nature of the dizziness, the rotatory sense was dominant in peripheral vertigo and the floating sense and blurred vision in central vertigo. Peripheral vertigo was triggered by position change of head and body, and central vertigo by the eye movement. Audiograms I showed that most patients with central vertigo had normal hearing, but 46.7% of thoswith peripheral vertigo had an abnormal hearing disturbance. Nystagmus was more prevalent in peripheral vertigo Conclusion: History taking and physical examination played an important role in the diagnosis of dizzy patients. An important part of the diagnosis of a dizzy patient could be to evaluate the peripheral origin, the central origin and others causes. Because central vertigo may be associated with a significant neurological pathology, spectral radiological studies, including MRI and CT, are mandatory to rule out a devastating brain lesion.

불량 단감을 이용한 속성 감식초의 제조와 품질 평가

정용진,신승렬,강미정,서지형,원충연,김광수 동아시아식생활학회 1996 동아시아식생활학회지 Vol.6 No.2

This study was performed to analyze general components such as, organic acids, alcohols and minerals in persimmon vinegar made with quick fermented using deteriorated sweet persimmon and four commercial vinegars(rice vinegar, apple vinegar, brewed vinegar, traditional persimmon vinegar) purchased in local markets for quality evaluation. The pH and total acidity of all vinegars were in the range of 2.02~3.02 and 4.62~9.78%, respectively. The pH of quick fermented persimmon vinegar(A) was relatively higher than that of others. Acidity was the highest in brewed vinegar. Total sugar content was in the range of 0.45~6.43%. These contents were high in were high in traditional persimmon vinegar wherase low in brewed vinegar. Total nitrogen and amino-nitrogen were in the range of 0.025~0.046% and 0.015~0.029%. Organic acids were identified as acetic acid, citric acid, tartaric acid, malic acid, succinic acid, formic acid and oxalic acid. While major minerals of all vinegars were Mg, Ca, and Na, minor minerals were Zn, Cu, Mn and Fe.

시판 및 속성 감식초의 이화학적 특성

정용진,서권일,김광수 동아시아식생활학회 1996 동아시아식생활학회지 Vol.6 No.3

Physicochemical properties of marketing and intensive persimmon vinegar were investigated, the results are as following: Total. acidity in D, H, S, J, sweet persimmon(SP) and persimmon vinegar were 4.20, 3.03. 2.58, 3.30, 5.92 and 5.81%, respectively, and the value of intensive persimmon(IP) vinegars were higher than that of others. SP sample was lower than any other vinegar as compared to L value in colors, no sample was lower than H and S vinegar in turbidity and browning. Brix of IP samples were lower than that of other vinegars, there was a little fructose in the all samples. Among the organic acids, galacturonic acid content except acetic acid was the highest, and ascorbic and malic acid were detected a little. Calium content was the highest of minerals in persimmon vinegars, phosphorus was detected a little. Alcohols of 5~6 types were analyzed in each samples, free amino acids of 17~24 types were analyzed, D and IP vinegars have more alcohols and amino acids, and their contents were higher than the others. Volatile components of 6~9 types were analyzed in each samples, these have more ethanols except acetic acids than other volatile components, and the contents of IP samples were higher than that of marketing persimmon vinegars.

도메인이름의 無斷占有 關聯 새로운 立法에 對한 小考

鄭震溶 법무부 2004 선진상사법률연구 Vol.- No.24

A Specific Domain name is only exsisting one through the world. Therefore as what the Domain name is, there is possibility which many people wants to register the Domain name at the same time. By not having restrict, the Domain name is registered by the rules of "first come, first served". And once it is registered, the man who registered it has a monopoly status and uses it over and over just by renual. Because of this, a conflict arises between the Domain name registrant and the trade mark possessors. The trademark possessors have tried to solve this problems by the Trademarks Law and the Unfair Competition Law. But these laws were not enacted for this Domain name dispute, The trademark possessors have been through so many difficulties till this time especially on the matter of cybersquatting. So Korea enacted the Internet Domain Name Law on 29 January 2004, and remanded the Unfair Competition Law on 20 January 2004 which restrict cybersquatting. This treatise is written to think about the problems that happen when the existing Trademarks Law and the Unfair Competition Law are applied to cybersquatting, the way that we construct new Internet Domain Name Law and Unfair Competition Law which is not applied to real case yet, the problems that new Internet Domain Name Law and Unfair Competition Law have. 상표권자에게 판매할 목적 또는 상표권자의 등록을 방해할 목적 등의 부정한 목적으로 상표권자의 상표와 동일 또는 유사한 도메인이름을 등록만 하였을 뿐 그 도메인이름 하에 웹사이트를 개설하지 않았거나 개설하였다 할지라도 웹사이트에 실질적인 내용이 없는 경우를 상표법, 부정경쟁방지법 등의 기존 법률로 규율할 수 있는가에 대하여 많은 논의가 행하여졌으나 학설, 판례는 이하에서 설명하는 것과 같은 이유로 이를 부정하는 것이 일반적이었다. 이에 2004. 1. 20. 법률 제7095호로 개정된 부정경쟁방지법 제2조 제1호 아목과 2004. 1. 29. 법률 제7142호로 제정된 인터넷주소자원에관한법률 제12조는 도메인이름의 무단점유에 대한 구제수단을 규정하고 있다. 이러한 시점에 쓰여진 이 논문은 도메인이름의 무단점유에 기존의 상표법, 부정경쟁방지법을 적용하면 어떠한 문제가 발생하기에 위와 같은 새로운 입법이 있었던 것인지를 살핀 후, 국내에서는 아직 적용 사례가 없는 부정경쟁방지법 제2조 제1호 아목, 인터넷주소자원에관한법률 제12조의 요건을 어떻게 해석할 것인가를 통일도메인이름분쟁해결방침(Uniform Domain Name Resolution Policy, 이하 "UDRP"라 한다), 반사이버스쿼팅소비자보호법(Anticybersquatting Consumer Protection Act, 이하 "ACPA"라 한다) 등의 적용사례에 비추어 생각하여 보고, 2중 입법의 의문, 등록이전청구권의 부재, UDRP결정에 대한 불복수단의 부재등 새로운 입법의 문제점에 대하여 생각하여 보는 것을 목적으로 작성되었다.

회맹장 및 대장을 침범한 장 결핵에 있어서 바륨을 이용한 방사선 이중조영촬영 소견에 대한 연구

진용현,전혜정,이용근,강철호,조성범,김윤환,박철민,차상훈,김일영,이혜경 건국대학교 의과학연구소 2002 건국의과학학술지 Vol.12 No.-

To determine the characteristic double contrast barium enema findings of ileo-cecal and colonic tuberculosis and to make differential diagnosis from other bowel inflammatory disease. Sixty-seven patients confirmed as ileo-cecal and/or colonic-tuberculosis in six hospitals were evaluated retrospectively. The diagnosis was proven by endoscopic biopsy, surgery, and clinical follow up. Nine of 67 patients were excluded because radiologic findings were normal though endocopic biopsy examinations resulted in tuberculosis. Histopathological diagnosis were madein 58 cases as follows: endoscopic biopsy(n=29), operation(n=12), follow up examination(n=14), tissue culture(n=1), laparoscopic biopsy(n=2). There were 30 men and 28 females. The mean age were 36 yrs(range:16-67), 34 patients were in the second and third decade. The distribution of lesions, morphological characteristics, mucosal changes, ulcer shape and depth, associated with other tuberculous lesion, multiplicity were retrospectively analyzed in the findngs of double contrast barim enema. The commonest clinical symptoms were abdominal pain(n=35, 60%) and diarrhea(n=18, 31%). The chest radiography revealed association of pulmonary tuberculosis in 39 cases. Tuberculous lesions of other organs except chest were present in five cases. Double contrast barium enema findings showed a thickening and irregularity of intestinal mucosal fold(n=29, 50%), bowel contraction and/or shortening(n=26, 45%). There were small, nodular, superficial ulcers in the case of ulcer depth less than 2 mm in the depth. As many as 39 cases(67%) in this series had two or more lesions in the intestinal tuberculosis. The involved segment revealed symmetrical feature(n=21, 36%). Inflammatory pseudopolypoid lesions were noted in 30 cases(52%). The ileo-cecal region was involved in 38 cases(66%). The ascending colon(n=25, 43%) was the second commonest site with or without contiguous cecal involvement. Lesions in terminal ileum, ileo-cecal valve, transverse colon, descending colon and appendix were seen in 19, 18, 18, 12 and seven cases, respectively. Among 18 cases of ileo-cecal valve lesions, there were incompetency in nine cases(16%) and wide gapping in four cases(7%). In 14 cases, tuberculous enterocolitis was diagnosed by clinical follow-up and double contrast barium enema study. 12 out of 14 cases, the findings of double contrast barium enema of tuberculous enterocolitis were normalized after anti-tuberculous medication. However, two cases showed more aggravated state than before due to inappropriate treatment. Double contrast barium enema plays an important role in the diagnosis of tuberculous enterocolitis, determines the type, site and extent of the involvement and helps in treatment guide by using sequential studies.

대구·경북지역의 전통발효식품 이용에 관한 실태조사

정용진,김주현 동아시아식생활학회 1996 동아시아식생활학회지 Vol.6 No.3

This survey was carried out to investigate the usage of traditional fermented foods. Questionaries were answered by 283 female adults ranging from 20 to 60 ages in Daegu city and Kyungbuk province. 39.72% of total subjects answered that they made Meju and soybean paste by themselves. There were significant differences according to income, residential area, education level, and family size. In the results of the preference and consumption study on traditional soybean pastes, Kimchi and salted sea foods, preference of salted sea foods was the highest, whereas the consumption of salted sea foods was the lowest. The recognition of soybean pastes and traditional foods was remarkably low. However, the consumption frequency of traditional fermented foods and the recognition of soybean pastes and traditional foods were significantly increased with age.

Bromelain과 Zn-Methlonnine 혼합 급여가 젖소의 산유량 및 체세포수에 미치는 영향

정유진,김용국 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2

Holstein cows(n=8) were assigned to control diet(n=4) and treatment diet(n=4) containing products of Bromelain(50g/kg) and Zn-methionine (133g/kg). Basal diet was mixed as total mixed rations with 60% concentrate and 40% roughage(rice straw) and fed for 8 weeks. The milk production, somatic cell counts in milk were measured and determined. The results were summarized as follow. Average milk production was higher for cows fed treatment diet(30.2kg/d) than cows fed control diet(29.6kg/d) (P<0.05). The somatic cell counts was significantly lower for cows fed treatment diet(179.8×10³/ml) than cows fed control diet (260.8x10³/ml)(P<0.05). In conclusion, supplementation of both Bromelain and Zn-methionine increased milk production and reduced somatic cell counts in milk. 본 실험은 사료 첨가제로써 Bromelain과 Zn-methionine의 급여가 착유우의 산유량 및 체세포 수 함량에 미치는 영향을 규명하고자 실시하였다. 홀스타인 착유우 8두(대조구 4두 및 처리구 4두)를 공시하여 대조구는 기본 사료를 급여하고 처리구에서는 Zn-methionine 133g/kg(Zn 20 및 methionine 46.7g)과 bromelain 50g/kg(br 30g/kg)을 경구투여하였다. 그리고 시험은 착유우의 비유초기에 8주간 실시하였다. 본 시험에서 얻은 결과는 다음과 같다. 두당 평균 유량은 대조구(29.6kg)에 비하여 처리구(30.2kg)에서 높게 나타났다(P<0.05). 우유내 체세포수는 대조구(260.8Xl0³ml)에 비하여 처리구(179.8X10³ml)에서 현저하게 낮게 나타났다(P<0.05). 따라서 이들 첨가물의 급여는 착유우의 산유량을 증가시키고 우유의 위생상 지표가 될 수 있는 체세포수의 감소를 가져오는 결과를 얻었다.

소화성 궤양 환자에 대한Lansoprazole의 치료 효과

정현용,김진희 최신의학사 1997 最新醫學 Vol.40 No.6