STD-11 와이어 컷 방전가공시 가공조건에 따른 북현상 고찰

조규재,김선진,조상현,김훈희 朝鮮大學校 機械技術硏究所 2000 機械技術硏究 Vol.3 No.2

From the experimental study of W-EDM for alloyed steel STD-11, the characteristics such as Hand Drum Form and surface roughness have been observed and evaluated for various conditions. In square hole, the increase of IP as to made condition, the calculate high value of surface roughness. Also compare dimensionless square hole with circle hole' graph. In circle hole, if a value of surface roughness IP 6 in a side of circle it show a 0.4㎛ and in IP 8, 0.6㎛, in IP 10, 0.7㎛, in IP 12, 0.8㎛ higher than before. This figure show the surface roughness is higher than before, because a table move in either X-axis or Y-axis in square hole, on the contrary, in circle there table move in X-axis and Y-axis at the same time, hand drum form getting small when wire tension increase 1000gf to 1500gf, at the same working conditions, the smaller of off time, the maller of hand drum form in same condition and same wire tension. but if you compare square hole with circle hole' graph, hand drum form displayed in maintained term of working condision, on the contrary, in case of square hole variation of hand drum form is more increase than a grow of IP

흰쥐 腦의 中心扁桃核에서의 Substance P 및 Enkephalin 함유구조의 형태학적 특징

마도훈,조희중,김은희,박매자,배용철,홍해숙,주강 慶北大學校 醫科大學 1990 慶北醫大誌 Vol.31 No.4

흰쥐 뇌의 중심편도핵에서의 substance P (SP) 및 Enkephalin (ENK) 함유신경세포 및 축삭종말의 분포를 관찰하기 위하여 peroxidase antiperoxidase법을 행하여 다음의 결과를 얻을 수 있었다. SP함유신경세포는 중심편도핵의 medial zone에 분포하였고 SP함유축삭종말은 lateral capsular zone에서 고밀도로 관찰되었다. SP함유신경세포는 원형 내지는 타원형이었고 2∼3개의 근위수상돌기(proximal dendrite)를 가졌다. ENK함유신경세포 및 축삭종말은 공히 central 및 lateral capsular zone에 존재하였다. ENK함유 신경세포는 직경이 약 17-24㎛ 정도였고 3∼4개의 근위수상돌기를 관찰할 수 있었다. The central amygdaloid nucleus is rich in a number of neuropoptides. The present study examines the distribution and morphological characteristics of substance P (SP) and leucine-enkephalin (ENK) containing neurons in this nucleus. The results obtained were as follows: SP immunoreactive cells were observed within the medial zone and SP immunoreactive axon terminals were heaviest within the lateral capsular zone. The immunoreactive cells were round or oval shape (diameter, 15-20㎛) and had two or three proximal dendrites. ENK immunoreactive cells and axon terminals located within central and capsular zones. The immunoreactive cells were round or oval shape (diameter, 17-24㎛) and had three or four proximal dendrites.

성상교 세포종에서 p53 유전자발현에 대한 면역조직화학적 연구

황금,한용표,조미연,정순이,김헌주 대한신경외과학회 1995 Journal of Korean neurosurgical society Vol.24 No.3

Nineteen astrocytic neoplasms, such as 9 case of glioblastoma multiforme, 6 case of anaplastic astrocytoma and 4 case of low grade astrocytoma, were analysed in view of the relationship between histopathologic grade, nuclear pleomorhism, grade of mutant p53 gene expression and mean survival time after operation. The histopathologic calssification by Ringertz and immunohistochemical stain for mutant p53 gene with the DO-7 anti-p53 oncoprotein mouse monoclonal antibody were applied, and the results obtained were as follows : 1) Among 19 cases, 18 cases were located in the supratentorium where 13 cases(42%) were located in the left and 17 cases(55%) in the right. 2) The p53 gene expression was detected in 12(63.2%) of the cases, as revealed by positive nuclear staining. All cases of glioblastoma multiforme showed p53 gene expression, compared with two(33.3%) cases of anaplastic astrocytoma and one(25%) case of low grade astrocytoma. The frequency and degree of histopathologic grade(p=0.03) 3) The mean survival time after operation was 29.49±4.08 months in case with p53-negative tumors and 12.93±3.14 months in cases with p53-positive tumors(p<0.05). 4) Both histopathological calssification and p53 gene expression showed a significant influence on servival (p=0.02 and p=0.03, respectively) 5) The relative risk for survival time was the highest in p53 gene expression. In conclusion, p53 gene expression appears to be one of the recommendable prognosticators among astrocytic neoplasms.

전유전체 서열 분석과 가시화를 위한 워크벤치 개발과 응용

최정현,진희정,김철민,장철훈,조환규 한국유전학회 2002 Genes & Genomics Vol.24 No.2

An increasing number of genome sequencing projects results in explosive growth of whole genome sequences. Furthermore the number of studies on the functions of individual genes has also been rapidly increased. However on-memory algorithms are not applicable to the analysis of whole genome sequences, since the size of individual whole gene a ranges from several million base pairs to hundreds billion base pairs. In order to effectively manipulate the huge sequence data, it is necessary to use the indexed data structure for external memory. In this paper, we introduce the development and application of the workbench for the analysis and visualization of whole genome sequences using string B-tree that is suitable for the analysis of huge data. This system consists of two main parts, the analysis query part and the visualization part. The query system supports various transactions such as pattern matching, k-occurrence, and k-mer analysis. The visualization system helps biologists to easily understand whole genome structure and specificity by various kinds of visualization such as whole genome sequence viewer, annotation viewer, CGR (Chaos Game Representation) viewer, k-mer viewer, RWP (Random Walk Plot) viewer, and map viewer. We can find the relationships among organisms, support gene prediction in a genome, and study the function of junk DNA using our workbench. In this paper, we apply our workbench to investigating specific sequence such as avoided sequence, common sequence, and classifiable sequence.

뇌종양의 악성도 판정을 위한 분열기 세포측정에 관한 연구

한용표,변진수,허철,홍순기,김헌주,조미연,정순희,김호근 대한신경외과학회 1992 Journal of Korean neurosurgical society Vol.21 No.11

Tissue markers of cellular proliferation have been utilized as prognostic indicators in tumors of the central nervous system. Nucleolar organizer regions represent transcriptionally active sites of ribosomal deoxyribonucleic acid and can be identified by a simple argyrophilic technique. The authors describe a standardized approach to the assessment of these argyrophilic nucleolar organizer regions(AgNORs) in 25 astrocytomas(including 6 glioblastomas), 6 oligodendrogliomas and 1 xanthochromic astrocytoma, which were also estimated in association with 2-year survival post-operatively. Appropriate sections were silver stained and AgNORs were counted in 1000 cells. In cases of astrocytoma, the mean AgNORs/cell count of group survived more than 2 years after operation was significantly different from that survived less than 2 years : 1.93±1.13 vs. ≥ 4.79±3.90. And the mean AgNORs/cell count was 1.53±0.32 in low grade astrocytoma. 2.03±0.59 in grade Ⅲ astrocytoma, and ≥6.64±3.77 in grade Ⅳ astrocytoma. Among the patients with the same grade astrocytoma, the mean AgNORs/cell count was lower in the cases of long survival rather than short survival. The assessment of AgNORs can be easily performed by any surgical pathology laboratory without specialized equipment. It may be used as a valuable and independent prognosticator of biological behavior in cases of astrocytoma.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1997)

김재홍,문득곤,김정수,김용준,임동진,박상훈,김희성,이민수,송기훈,김갑형,김형석,성소영,이인섭,김석우,황지환,조창근,김경문,부태성 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.3

Background : In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. Objective & Methods : For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results : In 1997. 99 strains of N. gonorrhoeae were isolated, among which 45(45.5%) were PPNG. Conclusion : The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 45.5% in 1997.

Identification of Pharmaceuticals for process control using Near Infrared Spectroscopy and Soft Independence modeling of Class Analogy (SIMCA)

Cho, Chang-Hee,Kim, Hyo-Jin,Maeng, Dae-Young,Seo, Sang-Hun,Cho, Jung-Hwan 한국근적외분광분석학회 2000 Near infrared analysis Vol.1 No.2

The identification step of raw drug materials is an indispensible procedure in the GMP manufacturing process within the pharmaceutical industry. However, wet chemistry methods for identification of drug materials, used by the various Pharmacopeia are time-consuming and expensive steps. In this paper, near-infrared spectroscopy (NIRS) has been developed for identifying eleven drug substances including calcium pantothenate, cefaclor, cefoperazone, cephradine, dextromethorphan, ehtambutol, nicotinamide, pyrozinamide, tramadol, vitamin C, and vitamin E. Also the aim of ths work is to consturct a new algorithm for calibration model using soft independence modeling of class analogy (SIMCA) with Malinowskis Indicator Function (IND), which is used for finding the number of principal components of each class of the SIMACA model. The use of NIR technique with pattern recognition to qualify raw materials can make it possible to monitor process in real time as well as to control all procedures in the pharmaceutical industry. As the result, the samples identified of 183 different batches from 11 different compounds were separated clearly by SIMCA with 2nd derivative spectra in the NIR region of 1100∼2400 nm.

생약 및 생약(한방)제제의 품질평가에 관한 연구(Ⅰ) : 기기분석법에 의한 길경의 지표성분탐색 Studies on the standard for standardization of Platycodon Root using the instrumental analysis

조정희,김도훈,오미현,강인호,심영훈,김은경,조창희,지선경,원도희,김영중,문영희 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

튿은 도라지 ,PfgfycodoH gian겉친orum A. De Candole 의 뿌리 또는 주피를 제거한 것으로 한방 제제에서 분즈파뼘 및 트늦촛랸 등의 효능으로 널리 사용되는 한약재이다. 현재 길경 함유 제제는 방풍통성산, 오적산 등 100꺽'품목치 허가되어 있으며 약전규격시험으로는 확인시험, 회분 및 엑스함량이 설정되어 있으나, 함랸시험은 설정되어 있지 않다. 따라서 본 연구에서는 생약 및 생약제제(한방제제) 의 품질규격화를 위하여 국내, 중국 및 일본 길경 유통품 30종을 확보하여 형래즉적 감별 및 약전규격 시험을 실시하였고, TLC법에 의한 확인시험법과 지표성분을 이용한 기기분석법에 의한 항량시험법을 확립하였다. 약전규격시끌으로서 회분, 엑스함량을 시험한 결과 회분은 1.4~4.0% (기준 : 4.0% 이하)이며 엑스함량은 쪼.3~T4.4% (기준 :25.0% 이상)으로 모두 적합하였다. 확인시험법을 검토한 결과 메탄올추출액을 흡착제 : Silicagel 9el GF2%, 전개용매 .클로로포름 ·메탄을 ·물 (6 :4 : 1), 발색제 : 10% 알코올성황산시액의 초건으로 박층크로마토그래프법에 파라 시험하였을 때 표준품 및 각 원료에서 가장 좋은 패턴을 나타내어 확인시험법으로 가장 적합하였다 함량시험의 경우 지표물질로 platycodin D에 대해 검토한_켤과 HPLC법으로는 피크의 검출이 용이하지 않았으며, HPTLC법에 따라 시험했을패 흡착제 .옥타데실실릴화한 실리카겔, 전개용매 :물 ·메탄을 sodium dodecyl sulfate (20 · 20 : 0.5), 발색제 : 10% 에탄올섣황산시액, 검출파장 : 450nm의 조건에서 최적의 결과를 나타내었다. 각 즌역별 유통품 30종에 대하여 platycodin D 함량을 측정한 결과는 0.48~l,24%(0.79±0.20%)이었고, 또한 길경단미엑스, 방풍통성삯, 오적산, 패독산, 인삼패독산 및 삼소음 등 길경함유제제 중 plat)·codin D의 추출이행률을 검토한 결과 45.g~93.2%(보.2±17.8%)의 추출이행률을 나타내었다. 본 시험법으로 시판되는 길경함유제제인 형개연교탕, 인삼패독산 제제즉 대해 시험한 결과 0.18~l,24%를 나타내어 제제에서도 적용가능할 것으로 사료되었다. This study carried out the standardization and quality control of Platycodon Root. It is osed in traditional meacines to relieve diarrhea, constipation, bloating, low energyIrveas, and damage from parasites. Also it is used for its expectorant qualities to dilate thebronchii. Platycodon Root contains vafous compounds of saponins such as platycodin A, B,0,D2, D3, polygalacln D, f:』 and platycodigenin, phytosterol such as α -spinasteroT, o -spinasterolglucoside, sfigmasta-7-fnol and inulin as polysaccharide. In this study, the contents ofplatycodin D as a standard compound are 0.48~l.24% with 30 samples collected from diflerentareas. Total ash content of Platycodon Root samples Is 1.4 ~4.0% and EtOH ertrac4 content is25.3 ~34.4%. The identification method was evaluated by TLC and quantitative method byHPTLC for Platycodon Roo'4. The proper conditions of 7LC were , absorbent : Silicagel gelGp2s4, solvent : chloroform · afeOH · HaO (6 : 4 : 1), spraying reagent : 10% HBSOf. To fuautifyPlatycodon Root, HPTLC me·thed was applied and the optimal analytical conditions of HPTLCwere as follows , absorbent : Cls, solvent : H2O · hfeOH · sDs (20.20 : 0.5), spraying reagent :10% H2s04, used wavelengtil : 450nm. The availability of platycodin D in water extract ofPlatycodon Root preparations was 45.9 ~93.2% and the contents of platyrodin D in PlatycodonRoot preparations in market such af In-sam-par-dok~ san and Hyung-kye- yun-kyo-tang were0.24% and 0.18%, respectively.

Overexpression of a novel regulator of p120 catenin, NLBP, promotes lung adenocarcinoma proliferation

Hee Kim, Chang,Nam, Hae-Seong,Hee Lee, Eun,Hun Han, Seung,Jung Cho, Hyun,Jin Chung, Hee,Soo Lee, Nam,Jin Choi, Suk,Kim, Hojoong,Seon Ryu, Jeong,Kwon, Junhye,Kim, Hong-Tae Taylor Francis 2013 Cell cycle Vol.12 No.15