Disinhibition in pathological pain

배용철,고형곤 대한구강해부학회 2020 대한구강해부학회지 Vol.41 No.1

Noxious and tactile stimuli are transmitted into the spinal cord or in the trigeminal nucleus before being delivered to the brain. Inhibitory interneurons are distributed throughout these relay regions and modulate sensory stimuli by inhibiting primary afferent terminals (presynaptic inhibition) and spinal interneurons (postsynaptic inhibition) by releasing γ-aminobutyric acid (GABA) and/or glycine. Regarding pathological pain, the spinal inhibitory interneurons are involved in hyperalgesia, allodynia, spontaneous pain, and referred pain. Disinhibition is the loss of inhibitory power mediated by the inhibitory interneurons, underlying these symptoms. Studies on global GABAergic neurons have shown that dysregulation of intracellular Cl− concentration, death of inhibitory interneurons, and change of electrophysiological properties are the mechanisms underlying disinhibition. Recent studies exploring the connectivity and function of each specific class of inhibitory interneurons using neurochemical markers will strengthen the basic knowledge of disinhibition in inhibitory interneuron and help develop novel and effective treatments for pathological pain.

33. 인체의 생식기계에서의 Vasoactive Intestinal Polypeptide(VIP) 및 Dopamine-β-Hydroxylase(DBH) 함유 축삭 종말의 분포

배용철,홍해숙,조희중,주강,정성광 대한체질인류학회 1990 대한체질인류학회 학술대회 연제 초록 Vol.33 No.-

치수유래 구심성 신경섬유의 삼차신경 감각핵군에서의 연접특성

배용철,이은희,최민기,홍수형,김현정,남순현,김영진 대한소아치과학회 2001 大韓小兒齒科學會誌 Vol.28 No.2

일차연접부위에서 악안면 영역에서 유래하는 유해자극의 전달 및 처리기전을 이해하고자 horseradish pe개xidase를 치수지배 구심성 신경섬유를 표식한 후 연수후각에서 미세구조 및 연절 양상을 분석하여 다음과 같은 결과를 얻었다. 표식종말은 소수의 큰 치밀소포가 관찰되는 종말 (S형) 및 다수의 치밀소포를 함유하는 종말 (LDCV형)등 2종류로 분류할 수 있었다. S형 및 LDCV형 표식종말의 연접양식은 유사하였으며, 다수의 표식종말이 1개 혹은 2개의 neurofile과 연접을 이루어 대단히 단순한 연접양상을 보였다. 표식종말은 가지돌기체 보다는 다수의 가지돌기가시와 연접을 이루는 빈도가 높았다. 표식종말이 세포체 및 이에 인접한 근심부 가지돌기와 연접하는 경우는 드물었으며, 소수의 표식종말에서 p-ending과 연접하는 경우를 보였다. 표식종말의 체적, 표면적, 사립체의 체적, neurofile과 접하는 면적, 활성부위의 면적, 단위표식종말당 연접소포의 수 및 연접소포의 밀도등은 넓은 범위의 계측치를 나타내었으며, 이는 5명 및 LDCV형 표식종말 사이에 유의한 차이가 없었다. 이상의 결과로 미루어보아 연수후각에서 치수유래 구심성 신경섬유 종말의 연접양식은 고유의 특징을 보이며 이는 신경회로의 기능과 밀접한 상관관계를 가지는 것으로 판단되었다. Little is known about processing mechanism of pain sensation of the oral cavity at the 1st synapse of trigeminal sensory nuclei. Serial ultrathin sections of tooth pulp afferent terminals, identified by the transganglionic transport of 1% wheatgerm agglutinin conjugated horseradish peroxidase, were investigated wih electron microscope. Quantitative ultrastructural analysis was performed on digitizing tablet connected to Macintoshi personal computer (software: NIH Image 1.60, NIH, Bethesda, MD). Labeled boutons could be classified into two types by the shapes of containing vesicles : S bouton which contained mainly spherical vesicles (Dia. 45-55 nm) and few large dense cored vesicles (Dia. 80-120nm), and LDCV bouton, which contained spherical vesicles as well as large number of large dense coredd vesicles. Most of the parameters on the ultrastruotural characteristic and synaptic organization of labeled boutons were similar between S and LDCV boutons, except shapes of containing vesicles. Majority of the labeled boutons showed simple synaptic arrangement. The labeled bouton were frequency presynaptic to dendritic spine, and to a lesser extent, dendritic shaft. They rarely synapsed with soma and adjacent proximal dendrite. A small proportion of labeled boutons made synaptic contacts with presynaptic pleomorphic vesicless containing endings and synaptic triad. Morphometric parameters of labeled boutons including volume and surface area, total apposed area, mitochondrial volume, active zone area, vesicle number and density showed wide variation and theme were not significantly different between S and LDCV boutons. The present study revealed characteristic features on ultrastructure and synaptic connection of pulpal afferents which may involved in transmission of oral pain sensation.

삼층감광막구조를 이용한 미세패턴의 전자빔 묘화

배용철,서태원,전국진 대한전자공학회 1994 전자공학회논문지-A Vol.31 No.10

The PMMA/Ge/AZ trilayer resist decreased proximity effect of backscattering electrons and corrected pattern distoration in order to from deep submicron patterns. In the experiment, the prosiemity effect is decreased by 11% and 30% for the case of 0.9$\mu$m and 1.7$\mu$m AZ, respectively, in trilayer resist compared to monolyer resist. also, the EID of 240$\AA$ Ge film is smaller than that of 500$\AA$ film by 365. 0.1$\mu$m line/space was formed in the 2000$\AA$ PMMA layer with the condition of dose 330${\mu}C/cm^{2}$ and of 150sec of develop time in MIBK : IPA (1:3) developer.

일반연제 발표 : 부인과종양학 ; 자궁경부암에서 SCC항원, CEA와 CA-125 종양표지물의 효용성

배용철,박일수 대한산부인과학회 1991 대한산부인과학회 학술대회 Vol.68 No.-

砂囊粘膜細胞의 增殖部位에 關하여

裵龍喆,朴海子,朱剛 慶北大學校 齒科大學 1989 慶北齒大論文集 Vol.6 No.3

It is widely accepted that the surface and mucous neck cells of the gastric mucosa in mammals arise form the lower pits and the neck of the glands. The surface mucous cells are finally extruded and lost after migrating upward and the mucosa neck cells are sloughed at their sistes of born. In chick gizzard mucosa, on the other hand, it has been known that the new cells are divided and differentiated from the stem cells in the fundus of the glands and eventually sloughed into the lumen. In order to clarify proliferation sites of gizzard mucosal cells and to calculate the cell proliferation parameters of these, 66 one day postnatal male chick were injected intraperitoneally with ^3H-thymidine at 6 A.M. and the autoradiogram was obtained by the dipping method using Kodak liquid emulsion NTB-3. The results were summerized as follows 1. The proliferation of gizzard mucosal cells was found to occur not only at the entire regions of the glands but also the lower pits. 2. The generation time calculated from the labelling index and and S phase duration was 156.8 hours. 3. The generation time calculated form the mitotic index and mitotic, phase duration was 180 hours.

흰쥐의 깨물근 감마 운동신경원에 연접하는 GABA 및 glycine 면역양성 신경종말의 정량적 분석

배용철,박국필,이효정 대한해부학회 2000 Anatomy & Cell Biology Vol.33 No.5

위삼차신경핵에서 일차구심성 신경섬유 종말부의 미세구조 및 연접양상

배용철,박국필,정경원,박매자,조희중 대한해부학회 1995 Anatomy & Cell Biology Vol.28 No.5

방사선조사가 악하선 미세혈관과 내피세포에 미치는 영향에 관한 실험적 연구

최갑식,최미,손정익,유영아,배용철 大韓口腔顎顔面 放射線學會 1994 Imaging Science in Dentistry Vol.24 No.1

The purpose of this study was to investigate the irradiation effects on the capillary and endothelial cell in the submandibular gland. Sprague-Dawley strain male rats were singly irradiated to their neck region with the dose of 5Gy and 10Gy by 6MV X-irradiation and sacrificed on the 6 hours, 12 hours, 1,3,7, and 14 days after irradiation. The authors observed the histological changes of the capillary at H & E and PAS staining under a light microscope, and also observed the ultrastructural changes of the endothelial cell using a transmission electron microscope. The obtaining results were as follows: 1. In the light microscopic examination, the capillary density was slightly increased on the 1 day after irradiation, and increased until the 7 days after irradiation. After then, capillary density was apparently decreased. 2. The reaction to PAS staining at acinar cells was decreased on the 6 hours after irradiation, and recovered on the 7 days after irradiation. But reaction was decreased on the 14 days after irradiation again, after then, gradually recovered with days. 3. In the transmission electron microscopic examination, mild proliferation of cytoplasmic process of the endothelial cell and reduction in luminal size were observed just after irradiation. After then, nuclear degeneration, marked proliferation of cytoplasmic process, thickened basal lamina, and numerous cytoplasmic vesicles were observed on the 1 day after irradiation. These changes were recovered to normal on the 14 days after 5GY group, but not with 10GY irradiation group. And destruction of endothelial cell and loss of basal lamina were not observed in both groups. 4. From the above results, reduction in luminal size, proliferation of cytoplasmic process and thickening of basal lamina were observed as the irradiation effects on the capillary and endothelial cell of the submandibular gland. And also, these changes may induce increase in capillary number and endothelial permeability by means of increase of cytoplasmic vesicle formation. The changes appeared earlier and more prominent in 10Gy irradiated group than in 5Gy irradiated group.

방사선조사에 따른 백서 악하선내 혈관 내피세표 미세구조의 조기변화

손정익,최갑식,류정수,배용철 大韓口腔顎顔面 放射線學會 1995 Imaging Science in Dentistry Vol.25 No.1

The purpose of this study was to investigate the early irradiation changes on the ultrastructure of the capillary endothelial cell in the rat submandibular glands. For the study, 110 Sprague-Dawley strain male rats were singly irradiated to their neck regions with the doses of 2Gy, 5Gy, and 10Gy by 6MV X-irradiation, and sacrificed on the 3 hours, 6 hours, 12 hours, 1 day, 3 days, 7 days, and 14 days after irradiation. The authors observed the histologic and ultrastructural changes of the capillary endothelial cell using the light and electron microscopes. The results were as follows: 1.In the light microscopic examination, the capillary dilation was observed on the 6 hours group and the capillary density was slightly increased on the 12 hours group after 2Gy and 5Gy irradiation. And luminal size and capillary density were decreased on the 3 days and the 7 day groups after irradiation, after then, they were recovered. But capillary density was still decreased on the 14 days group after 10Gy irradiation. 2.In the transmission electron microscopic examination, the mild proliferation of cytoplasmic process of the endothelial cell and reduction in luminal size were observed on the 3 hours group after irradiation. After then, endothelial swelling, marked proliferation of cytoplasmic process, thickened basal lamina, and numerous pinocytotic vesicles were observed after the 1 day group after irradiation. Thickened basal lamina and numerous pinocytotic vesicles were still observed until the 7 days group after irradiation. These changes were recovered to normal on the 14 days group after 2Gy and 5Gy irradiation, but not after 10Gy irradiation. 3.In the scanning electron microscopic examination, the dilation of conduits and constriction, and meandering were observed on the 1 day group after 10Gy irradiation. These changes were observed with increased coarseness of the surface of the vascular resin casting on the 3 days group after irradiation. 4.From the above results, endothelial swelling, proliferation of cytoplasmic process, and thickening of the basal lamina appeared before the 6 hours group after irradiation. And these changes may also induce the increase of the capillary number and luminal size, after then, capillary permeability was increased via the increase of the number of pinocytotic vesicles. The changes were observed earlier and more apparent with the increase of the irradiation doses under the dose of 10Gy irradiation.