Serial values for hematologic and biochemical analysis after myocardial infarction in rats

Lee, Mi-Jin,Tae, Hyun-Jin,Li, Ying-Hua,Yu, Do-Hyeon,Han, In-Ae,Lee, Seok-Won,Ahn, Dong-Choon,Kim, In-Shik,Park, Jin-Ho The Korean Society of Veterinary Service 2008 韓國家畜衛生學會誌 Vol.31 No.2

To diagnose acute myocardial infarction (MI), many cardiac markers have been used in hematologic and biochemical analysis, and many studies have been published for hematologic and biochemical analysis associated with human acute MI. However, after occurrence of acute MI, the serial investigation for values in hematologic and biochemical analysis including chronic MI has rarely been performed. To observe the change of the serial values in hematologic and biochemical analysis, we induced artificial MI. The left main descending artery (LMDA) of the left coronary artery was ligated during the progression (day 1, 3, 5, 7, 14 and 30) of MI. Total 66 Sprague-Dawley rats were divided into the sham group (n=24, thoracotomy without LMDA ligation) and the experimental (MI) group (n=42, with LMDA ligation). And all individual in each group was sacrified at day 1, 3, 5, 7, 14 and 30 for the hematologic and biochemical analysis. In comparison of hematologic analysis between the sham and MI groups, the mean values of red blood cell (RBCs), hemoglobin and hematocrit (HCT) showed a steady increase. In biochemical analysis, the mean values of glucose, cholesterol, total creatine kinase (CK) and isoenzyme MB, and lactate dehydrogenase (LDH) were increased in all MI groups compared with the sham groups. The results of this study suggest that early hematologic and biochemical mean values occurred after acute MI are similar to those of human acute MI. In conclusion, we could observe the alterations and serial values in hematologic and biochemical analysis to the extent of chronic status after acute MI.

Serial values for hematologic and biochemical analysis after myocardial infarction in rats

( Mi Jin Lee ),( Hyun Jin Tae ),( Ying Hua Li ),( Do Hyeon Yu ),( In Ae Han ),( Seok Won Lee ),( Dong Choon Ahn ),( In Shik Kim ),( Jin Ho Park ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.2

To diagnose acute myocardial infarction(MI), many cardiac markers have been used in hematologic and biochemical analysis, and many studies have been published for hema-tologic and biochemical analysis associated with human acute MI. However, after occurrence of acute MI, the serial investigation for values in hematologic and bioche-mical analysis including chronic MI has rarely been performed. To observe the change of the serial values in hematologic and biochemical analysis, we induced artificial MI. The left main descending artery(LMDA) of the left coronary artery was ligated during the progression(day 1, 3, 5, 7, 14 and 30) of MI. Total 66 Sprague-Dawley rats were divided into the sham group(n=24, thoracotomy without LMDA ligation) and the experimental(MI) group(n=42, with LMDA ligation). And all individual in each group was sacrified at day 1, 3, 5, 7, 14 and 30 for the hematologic and biochemical analysis. In comparison of hematologic analysis between the sham and MI groups, the mean values of red blood cell(RBCs), hemoglobin and hematocrit(HCT) showed a steady increase. In biochemical analysis, the mean values of glucose, choles-terol, total creatine kinase(CK) and isoenzyme MB, and lactate dehydrogenase(LDH) were increased in all MI groups compared with the sham groups. The results of this study suggest that early hematologic and biochemical mean values occurred after acute MI are similar to those of human acute MI. In conclusion, we could observe the alterations and serial values in hematologic and biochemical analysis to the extent of chronic status after acute MI.

영남 지역 시첩(詩帖)제작의 한 국면 - 구암(龜巖) 이정(李禎) 가문의 『수서시(壽瑞詩)』를 중심으로 -

이미진 ( Lee Mi-jin ) 우리한문학회 2021 漢文學報 Vol.45 No.-

『壽瑞詩』는 李稵(1372~1495)의 장수를 기념하여 당시 右議政이었던 姜孟卿(1410~1461)이 지어준 祝壽詩및 후대의 次韻詩를 모아둔 詩帖이다. 이 시첩은 李稵의 후손인 龜巖李楨(1512~1571)이 제작을 주도하였는데, 여기에는 강맹경의 元韻이 지어진 作詩현장과 이후 여러 대에 걸쳐 지어진 次韻詩의 수습 과정이 그대로 남아있다는 점에서 눈여겨볼만하다. 『수서시』는 구암 한시를 모아둔 시집이 아니며, 문학적 완성도를 운운하기 위한 작품 선집도 아닌 만큼 이 시기 영남 지역을 중심으로 활동한 지식인들의 한시 授受장면을 엿볼 수 있는 흥미로운 자료라 할 수 있다. 구암 이정이 오랜 기간 영남 지역에서 지방관을 역임했다는 사실과 그 과정에서 차운시를 수습했던 사실에 주목하여 『수서시』의 제작 경위 및 차운시 현황을 살펴본 결과 차운시는, 강맹경 생존 당시, 구암의 지방관 재직 시, 그리고 구암 사후 수습되었다는 사실을 알 수 있었다. 구암은 영천군수(경북 영주)에 제수된 이후 경북 선산과 충북 청주를 거쳐 경북 경주와 전남 순천에 이르기까지 영남의 상하를 관통하면서 비교적 大邑의 수령을 지냈는데, 이 가운데 영주, 선산, 청주 지역에서 재직 시 요청하여 받은 차운시가 수록되어 있기 때문이다. 선대의 시문을 후손이 모아 제작했다는 점에서, 그리고 차운시의 대부분이 선조의 장수와 그의 후손들의 효행을 칭송하고 있다는 점에서 『수서시』는 명실상부한 구암 가문의 기록물의 성격을 지니고 있다고 할 수 있다. The 『Suseosi(壽瑞詩)』 is Sicheop(album of poems, 詩帖) collecting Chuksusi(祝壽詩) created by Gang Maenggyeong(姜孟卿, 1410-1461), the contemporary Uuijeong(state councilor), which expresses the longevity of Lee Ja(李稵, 1372-1495) from Sachoen(泗川) Lee’s family and Chaunsi(次韻詩) created by the next generation. This Sicheop, which was primarily created by Guam Leejeong (龜巖李楨, 1512-1571), a descendent of Lee Ja, shows the site in which Gang Maenggyeong’s Wonun(元韻) was created and the process in which Chaunsi which had been created across many generations was learned. The 『Suseosi』 is neither the collection of Guam’s Chinese poems nor an anthology with literary completion, but may be an interesting material with which it is possible to get a glimpse of the scenes in which intellectuals, who engaged in literary activities around Yeongnam province, exchange Chinese characters with each other. An analysis on the creation process of the 『Suseosi』 and the status of Chaunsi by focusing on the fact that Guam Lee Jeong served as a local official in Yeongnam province for a long time and learned Chaunsi in the process, shows that Chaunsi had been learned by his descendents, during the survival of Gang Maenggyeong and the period in which Guam served as an local official, and after the death of Guam. After being appointed as a Yeongcheon(Yeongju, Gyeongbuk) country headman, Guam held the post of a local governor in relatively bigger towns(大邑), by vertically penetrating Yeongnam, from Seonsan, Gyeongbuk and Cheongju, Chungbuk to Gyeongju, Gyeongbuk and Suncheon, Gyeongnam. The Chaunsi he asked to receive when he held office in Gyeongju, Seonsan and Cheongju was contained in the 『Suseosi』. The 『Suseosi』 is the de facto record of Guam's family, in that the descendants created it by collecting the ancestors’ poetic works and most of Chaunsi praise the ancestors’ longevity and his descendants’ filial conduct.

Transcriptional activation of hypoxia-inducible factor-1α by HDAC4 and HDAC5 involves differential recruitment of p300 and FIH-1

Seo, Hee-Won,Kim, Eun-Jin,Na, Hyelin,Lee, Mi-Ock Elsevier 2009 FEBS letters Vol.583 No.1

<P><B>Abstract</B></P><P>The interplay between hypoxia-inducible factor-1α (HIF-1α) and histone deacetylase (HDACs) have been well studied; however, the mechanism of cross-talk is unclear. Here, we investigated the roles of HDAC4 and HDAC5 in the regulation of HIF-1α function and its associated mechanisms. HDAC4 and HDAC5 enhanced transactivation by HIF-1α without stabilizing HIF-1α. HDAC4 and HDAC5 physically associated with HIF-1α through the inhibitory domain (ID) that is the binding site for factor inhibiting HIF-1 (FIH-1). In the presence of these HDACs, binding of HIF-1α to FIH-1 decreased, whereas binding to p300 increased. These results indicate that HDAC4 and HDAC5 increase the transactivation function of HIF-1α by promoting dissociation of HIF-1α from FIH-1 and association with p300.</P><P><B>Structured summary:</B></P><P>MINT-6802187:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>FIH1</I> (uniprotkb:Q9NWT6) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802058:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>HDAC4</I> (uniprotkb:P56524) by <I>pull down</I> (MI:0096)</P><P>MINT-6802021:</P><P><I>HIF1 alpha</I> (uniprotkb:Q61221) <I>physically interacts</I> (MI:0218) with <I>HDAC4</I> (uniprotkb:P56524) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802036:</P><P><I>HIF1 alpha</I> (uniprotkb:Q61221) <I>physically interacts</I> (MI:0218) with <I>HDAC5</I> (uniprotkb:Q9UQL6) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802102:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>HDAC5</I> (uniprotkb:Q9UQL6) by <I>pull down</I> (MI:0096)</P><P>MINT-6802121, MINT-6802156:</P><P><I>P300</I> (uniprotkb:Q09472) <I>physically interacts</I> (MI:0218) with <I>HIF1 alpha</I> (uniprotkb:Q16665) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P>

Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

알코올의 섭취가 흰쥐의 혈액과 간의 지질대사에 미치는 영향

이명숙,김영연,김효숙,김매하,전진순,서진숙,송선미,양영재,윤원손,이미라,이효영,정승현 誠信女子大學校 生活文化硏究所 2004 生活文化硏究 Vol.18 No.-

To investigate the effects of ethanol on the lipid metabolism in blood and liver, twenty one male Sprague Dawley rats had orally taken the 25% alcohol and 50% alcohol by 5g per Kg of weight during 3 weeks after the adjusting period. For the energy balance, 85% sucrose and 45% sucrose was added in control group and 25% alcohol group each. The weight was reduced by alcohol treatment according to the levels, otherwise the weight of liver and heart was little increased in alcohol groups rather than in control without significance. Moreover, RBC, WBC, hemoglobin and hematocrit were increased by alcohol treatment according to the levels without significance. Otherwise, adjusted bilirubin levels by 100g of weight was significantly increased by alcohol treatment dose dependently. Alcohol induced RBC degradation and increased circulated bilirubin because of liver damage. For blood lipid profiles, blood cholesterol was increased in 50% of alcohol group compared to that of 25% and control groups, otherwise, blood triglycerides (TG) was increased by 30% in 25% alcohol groups rather than in control but not significantly different. The hepatic lipid composition, cholesterol levels in alcohol treatment groups was lower than those in control. The hepatic TG was not influenced by alcohol treatment. The two reasons of decreasing hepatic lipids were suggested by 85% sucrose of control for energy balance more synthesized cholesterol and TG than those in 25 or 50% alcohol groups, and by cholesterol and TG could not be synthesized because of liver damage from alcohol.

임상 검체에서 분리된 nontyphoid Salmonella에서의 integron의 빈도와 특징

이정은,박수진,김성한,김미나,이남용,이복권,이상오,김양수,우준희,최상호 대한감염학회 2009 감염과 화학요법 Vol.41 No.3

Background : Nontyphoid Salmonella (NTS) is a leading cause of human food-borne enteritis, It has been known that integron, a naturally occurring gene capture and expression element, Plays an important role in the development and dissemination of multidrug-resistance. In this study, we investigated the prevalences and molecular characteristics of integrons in NTS clinical strains. Materials and Methods : Between 1995-96 and 2000-03, a total 261 NTS clinical strains comprising 39 serotypes were collected from clinical specimens. All strains were serotyped, and the MICs of ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, tetracycline, and trimethoprim were determined by agar dilution method. Integrons were detected by PCR amplification of integrase genes, and gene cassettes were determined by PCR and sequencing, Conjugation experiments were performed using E. coli J53 as a recipient. The clonal relationship was analyzed by pulsed-field gel electrophoresls (PFGE). Results : Of the 261 strains tested, class 1 integrons were present in 21 strains (8.0%). Class 2 and class 3 integrons were not found. The integron-positive rate was higher in S. Typhimurium (24.2% [8/33]) than in S. Enteritidis (2.0% [3/153]). Overall rates of antimicrobial resistance were higher in integron-positive strains. dhfr12-orfF-aadA2gene cassette was detected in 5 strains, aadA2in 4 strains, dhfr17-orfF-aadA5 in 2 strains, and addA1 in 1 strain. Ten integron-positive transconjugants were successfully selected. Among 8 integron-positive strains of S. Typhimurium, 7 had similar PFGE patterns. Conclusion: This study suggests that integrons are already playing a significant role in antimicrobial resistance in NTS. Continuous monitoring is needed to detect the emergence and spread of integron-mediated antimicrobial resistance.

간암 환자에 합병된 Citrobacter braakii 패혈증 1례 보고

이해미,배성하,이수남,이정우,신아영,류시영,이수연,윤문희,한선영,한남익,이영석,유진홍 대한감염학회 2010 감염과 화학요법 Vol.42 No.3

Citrobacter species is a gram-negative bacilli that can cause opportunistic infections in immunocompromised hosts. Citrobacter braakii refers to the genomospecies 6 of the Citrobacter freundii complex. There are no detailed studies on infections caused by this newly identified specific genetic species in Korea. We herein report a case of a patient with hepatocellular carcinoma who, after undergoing transcatheter arterial chemoembolization, developed biloma which later progressed to C.braakii sepsis and did not respond to treatment. To our knowledge, this is the first reported case in Korea on C. braakii infection resulting in septic shock in a patient with malignancy in Korea.

일개 대학병원에서 일년간 분리된 반코마이신 내성 장구균의 임상 분자역학적 연구

송진영,김창억,김성욱,우흥정,김미란,이규만,이란,장미화,정희진,김우주 대한화학요법학회 2001 대한화학요법학회지 Vol.19 No.4

목적 : 한강성심병원에서는 2000년 3월 반코마이신내성 장구균이 처음 분리되어, 원내전파를 막기 위한 노력을 하였으나, 분리가 지속되었다. 따라서 이에 대한 좀더 자세하고 객관적인 자료를 얻기 위해 본원에서 분리된 반코마이신내성 장구균에 대한 임상분자 역학적 연구를 시행하게 되었다. 방법 : 2000년 3월부터 2001년 2월까지 한림대학교 한강성심병원에서 분리된 장구균을 대상으로 디스크 확산법, 최소발육억제 농도등의 측정을 통해 반코마이신 내성여부를 확인하였으며, 반코마이신내성 장구균을 대상으로 PFGE를 시행하여 형별 분석을 하였다. 결과 : 분리된 균주는 총 32균주로 모두 E. faecium이었다. 항생제 내성 검사 결과 모두 Van A형이었다. PFGE 결과 1a형이 8검체, 1b형이 5검체, 2a형이 4검체, 2b형이 4검체, 3a형이 2검체, 3b형이 5검체였고, 그 외 4, 5, 6형이 각각 1검체씩이었다. 총 32균주 중 56%(18/32)의 균주가 같은 시기, 같은 병실에서 동일한 PFGE 형을 보이면서 분리되었다. 결론 : 반코마이신내성 장구균의 PFGE 분석 결과 여러 종류의 반코마이신내성 장구균이 유행하였음을 알 수 있다. 그러나 같은 기간, 같은 병실에서 분리된 균주의 PFGE 형이 같은 것이 상당수 있었다. 이는 반코마이신내성 장구균의 원내 전파가 있었음을 의미한다. 따라서 반코마이신내성 장구균의 전파의 발생 및 전파 방지를 위해서는 보다 효과적인 감염관리 활동이 필요할 것으로 생각된다. Background: Vancomycin-resistant enterococci(VRE) have been a rising problem worldwide. In March 2000, VRE was first isolated from a patient in Hangang Sacred Heart hospital. Although efforts to prevent transmission of VRE were performed, isolations continued. So molecular epidemiological study of VRE was done. Method : The minimal inhibitory concentration(MIC) to vancomycin and teicoplanin, and pulsed field gel electrophoresis(PFGE) pattern of VRE isolated from March 2000 to February 2001 were evaluated. Results : 32 VRE were isolated. All of the VRE were Enterococcus faecium and showed Van A resistance phenotype. We found out that a significant number of VRE isolated during same period and in same room, were same PFGE patterns. (18 cases/ 32 isolates= 56%) Conclusion: This study demonstrated the spread of VRE of same PFGE patterns. It suggests the nosocomial spreads of VRE.

조혈모세포이식 환자에서 발생한 Cytomegalovirus 질환의 특징 : 일개 대학변원에서 최근 10년간의 경험

최수미,이동건,박선희,김시현,김유진,민창기,김희제,이석,최정현,유진홍,김동욱,이종욱,민우성,신완식,김춘추 대한감염학회 2009 감염과 화학요법 Vol.41 No.1

Background : Studies on cytomegalovirus (CMV) diseases in Korean hematopoietic stem cell transplant (HSCT) recipients are lacking and do not reflect the recent trends of advances and changes. Therefore, we tried to analyze the clinical features of CMV diseases in HSCT recipients over the past 10 years at a tertiary university hospital in Korea. Methods : Retrospective review of medical records was done for all adult HSCT patients who received transplant at the Catholic HSCT Center from January 1998 to January 2008. Results : Forty-four cases (2.2%) of CMV diseases were identified. CMV pneumonia was diagnosed in 17 patients, retinitis in 16 patients, enterocolitis in 7 patients, esophagitis 1 patient, gastritis in 1 patient, duodenitis in 1 patient, and hepatitis in 1 patient. The median onset of symptom was 90 days after transplantation. Late CMV diseases accounted for 47.7%. CMV related death varied from 0 to 58.8% according to the involved organ. CMV retinitis was diagnosed relatively later in the course of transplantation mostly in patients who had chronic graft versus host disease (GVHD). On the contrary, CMV enterocolitis mainly occurred in patients who suffered from acute GVHD. The overall concurrent CMV reactivation was documented to be 63.6%: the concurrent CMV reactivation was observed only in 37.5% of patients with retinitis. Conclusions : We observed some differences in the pattern of CMV disease manifestation according to the involved organ and reconfirmed the fact that CMV pneumonia is the most common and fatal disease in HSCT recipients. Additionally, CMV retinitis was not uncommon in HSCT recipients. Since specific marker does not exist in predicting retinitis, regular ocular examination should be done thoroughly, especially in patients with chronic GVHD.