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The result of studying the epidemiological characteristics of Salmonella strains which have been isolated from the domestic animals in Gyeongbuk province from February 1998 to August 2000 were summarized as follows. The isolation rates of Salmonella strains were 2.0% from cattle feces, 6.3% from cattle lymph node, 9.5% from pig feces, and 25.1% from pig lymph node. In poultry, the isolation rates were 30.3%. The isolates of Salmonella showed positive reaction for MUCAP test, methyl red test, but showed negative reaction for urea test, indole test, Voges Proskauer test. On TSI agar, the isolates showed acid butt, alkaline slant. Also, the isolates were identified as Salmonella strain by API 20E kit. Non H2S production Salmonella strains isolated from poultry were identified as S gallinarum. As a result of serotyping, B group were the most common in cattle and pig, D1 in chickens. 21 serovars were found. The common serovar from the domestic animals was S typhimurium, S derby, S agona, S schwarzenground, S enteritidis and S gallinarum. The most commonly encountered serovars in cattle were S agona and S typhimurium in pig, S gallinarum in chicken. As a result of antimicrobial susceptibility test, all Salmonella isolates were susceptible to amikacin, ciprofloxacin. Norfloxacin, cefotaxime and polymycin B. The resistance rates to tetracycline and streptomycin was 58% and 56% respectively. 69.3% of all isolates were resistant to more than one antimicrobial agent. Out of the resistant isolates, the isolates resistant to streptomycin and tetracycline was 36%. There were 24 strains of multiresistant isolates resistant to more than 5 antimicrobial agents. S typhimurium were resistant to all antimicrobial agents, also had a lot of multiresistant strains. Therefore, S typhimurium was considered as a major agent of antimicrobial resistance.
Porcine epidemic diarrhea virus (PEDV), an enveloped single stranded RNA virus in the family Coronaviridae, causes acute viral enteric disease in piglets. Recently outbreaks of porcine epidemic diarrhea (PED) have been rare in Europe but frequent in Asia. In Korea, the increase of PED prevalence is showing specially in postweaning pigs. The purpose of this study was to investigatenucleotide sequence of nucleocapsid protein gene of PEDV field isolates from postweaning pigs in Korea and get more information about the viruses. A total of 15 postweaing pigs clinically suspected of PEDV infection by severe watery diarrhea and dehydration were used in this study. Viral RNA was extracted from small intestines and stools of the pigs. The N gene was amplified by nested RT-PCR, purificated, sequenced, analyzed and then compared with published sequences of other PEDV strains. Three PEDVs were isolated from the suspected postweaning pigs. The N gene of three PEDV field isolates consisted of 483 nucleotides. These PEDV field isolates showed nucleotide sequence homology range from 99.6% to 95% with Chinese strains, from 99.8% to 95.2% with Korean strains, from 97.3% to 95.7% with Japanese strains and from 96.5% to 95.7% with Belgium and British strains. The encoded pritein shared range from 98.8% to 95.6% with Chinese strains, from 99.4% to 95% with Korean strains, from 97.5% to 96.3% with Japanese strains, from 95.6% to 95% with Belgium and British strains. By phylogenetic tree analysis based on nucleotide sequence, three PEDV field isolates were clustered into two groups which were Chinese isolate groups and other Korean isolate groups. These results indicated that some of PEDV field isolates prevailing in Korean postweaning pigs may be associated with those of Chinese strains and other Korean strains.
Nucleotide sequences of mitochondrial DNA (mtDNA) of 19 leopard cats (Prionailurus bangalensis euptilura) obtained from Seoul grand park zoo in South Korea were determined for analysing genetic diversity. In the leopard cats, 3 haplotypes of the partial cytochrome b sequences (603 base-pairs, bp) were identified. Haplotypes obtained from those genes showed existences of at least 3 maternal lineages of leopard cats in Korea. Tamura-Nei nucleotide distance among 3 haplotypes were 0.00. Molecular phylogenetic tree showed the similar clustering of haplotypes for genes. Meanwhile, no individual variations within the leopard cats in S. Korea. Genetic surveillance system of leopard cats in S. Korea is warranted for maintaining biological conservation.
The result of studying the pathogenicity of Salmonella typhimuriwn and S enteritidis isolated from domestic animals in Gyeongbuk province were summarized as follows. In Congo-red binding test, S typhimurium had much more rough types than S enteritidis. In colicin production test, 4 strains of S typhimurium were positive but all of S enteritidis were negative. In hemolysin production test, all of S typhimurium and S enteritidis were negative. In Guinea pig serum resistant test, all of S typhimurium and S enteritidis were positive. As a result of pathogenicity test to mice, 54.4% of mice were died. Therefore, S typhimurium and S enteritidis were considered as highly pathogenic. S typhimurium DT104 and S enteritidis PT4 were more pathogenic to mice than other phage types of same serovar. S typhimurium and S enteritidis were considered not so pathogenic for 6-day-old chickens. The recovery rates of Salmonella strains from mice and chickens inoculated were 96.8%, and 54%, respectively. In chickens, proportional to the time From 2 weeks after challenge inoculation. The recovery rates were noticeably decreased.
Korean goral (Neamorhedus caudatus) is registered as a natural monument number 217 by South Korea Cultural Heritage Administration. It is also recognized as the endangered speciesⅠ by Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). In this study, blood samples of Korean goral were collected to make reference intervals. Blood sampling was conducted on 19 numbers of Korean gorals (ten females, nine males), which were reared in Korean Goral Restoration Center. For total samples, the reference intervals of hematological parameters were: white blood cell 7.69∼10.87 K/μA; hematocrit 36.73∼46.18%; red blood cell 10.72∼12.86 M/μA; hemoglobin 12.79∼15.14 g/dL; mean corpuscular volume 33.15∼36.75 fL; mean corpuscular hemoglobin 11.53∼12.23 pg; mean corpuscular hemoglobin concentration 32.64-35.91 g/dL and red blood cell distribution width 39.2 ∼43.46%. For total samples of each parameters, the following results were obtained for serum biochemistry: glucose 111.81∼153.77 mg/dL; blood urea nitrogen 22.35∼28.91 mg/dL; creatine 1.22∼1.84 mg/dL; phosphate 4.57∼6 mg/dL; calcium 8.7∼9.1 mg/dL; total protein 6.53∼6.92 g/dL; albumin 3.1∼3.48 g/dL; globulin 3.26∼3.62 g/dL; alanine aminotransferase 56.7∼158.56 U/L; aspartate aminotransferase 230.35∼473.06 U/L; alkaline phosphatase 178.06∼332.47 U/L; gamma-glutamyl transpeptidase 131.6-∼181.24 U/L; total bilirubin 1.47∼2.12 mg/dL; cholesterol 46.48∼71.52 mg/dL; amylase 16.3∼26.03 U/L; sodium 150.43∼153.88 mmol/L; potassium 3.98∼4.6 mmol/L and chlorine 109.48∼113.26 mmol/L. The ranges of values were similar campared to previous studies except in the case of RDW value, which showed higher range than the RDW value of a previous study. The reference intervals from this study will be useful data for treatment and management of gorals.
Total viable cells and lactose non-fermenting cells were counted from animal feedstuffs (n=65). And isolation of Gram negative lactose nonfermenting enterobacteria and antibiotics susceptibility of isolates were performed. 1. The ranges of total viable cells / lactose non fermenting in animal feedstuffs from Korean cattle were counted as 9×10-1×10/1×102-6×10, milking cow as 1×104-2×108/2×102-8×10, pig as 1×104-1×106/2×102-6×104, and chicken as 7×l04-1×109/4×102-1×105 cfu/g, respectively. 2. Among the 214 isolates from feedstuffs, 87 from Chinan(n=23), 66 from Changsu (n=23) and 61 from Mooju(n=19) were isolated. Of these isolates, 60 from pigs (n=19), 51 from milking cows(n=15), 45 from chikens(n=11) and 58 from Korean cattle(20) were isolated. 3. Among the 6 genuses of Gram negative lactose nonfermenting enterobacili, Sal-monella sp, Y pseudotuberculosis, Ent agglomerans and Sal choleraesuis were frequently encountered. 4. A majority of isolates were sensitive to 19 antibiotics, singly or in combination. These isoates were completely susceptible to Cp, Gm, Imp and Pi, 93% to Ak and To, 73% to Cax and Ts, 66% to Cit and Tim, 46-53% to Caz, Cf and Cz, 33-40% to Am, Azt, Cfz and Ti, and 6% to Cfx, in order, but not susceptible to Crm. 5. Among the antibiotic resistant strains, a total of 23 resistant patterns was noted, and of these Crm 40(18.7%), Am Cf Cfx Cfz Crm Ti 27(12.6%), each of Azt Ctx Crm and Azt Cax Caz Cft Cfx Crm 22(10.3%) were frequently encountered.
정경훈 ( Kyung Hun Jeong ), 정미영 ( Mi Young Jeong ), 박해철 ( Hae-chul Park ), 아킬후세인 ( Md Akil Hossain ), 김대균 ( Dae Gyun Kim ), 이광직 ( Kwang-jick Lee ), 강정우 ( Jeong Woo Kang ) 한국가축위생학회 2017 韓國家畜衛生學會誌 Vol.40 No.4
The aim of this study is to develop an optimal analytical method for gonadorelin, progesterone, oxytocin and estradiol, the major components of hormones. A relatively simple and reproducible method using high performance liquid chromatography was developed and as a result of the measurement of specificity, linearity, repeatability, accuracy and intermediated precision, the validity of the developed method was verified with the result of meeting the verification criteria of analytical method validation. Using this newly developed method, 12 post-market veterinary products were tested and the ingredient content were 91.9∼116.4%, which satisfied the 90∼120% condition of the administrative measure standard. Therefore, if the newly developed method is used for the collection examination of hormone in veterinary medicine, it can be useful as an approved test method.
Forty-five Salmonella typhimurium isolates were encountered 8 phage types in which DT197 and U302 were the predominant types. The DT104 type which was first found from pig in Korea, and was resistant to chloramphenicol, streptomycin, sulfamethoxazole/trimethoprim, tetracycline, gentamicin and nalidixic acid. Twenty-two S enteritidis isolates were encountered 5 phage types in which PT4 were the representative (predominant). S enteritidis isolates were susceptible to all antimicrobial agents. As a result of PFGE analysis for S typhimurium and 5 enteritidis, PFGE patterns was better than phage typing in discriminating of strains. PFGE patterns were not in accord with phage type even though some strain had the same phage types.
Observations were made on the infection rate of common internal parasites in Jindo dogs from January to March, 1989. Fecal samples were collected from 115 Jindo dogs in Jindo area. On the basis of egg counting, the following results were obtained; 1. Of 115 fecal samples, 65.2% were appeared as positive. Dogs from intensive dog-rearing places have lower infection rates than others. 2. Of the parasites detected, Toxocara canis was the most commonly encountered(26.1%). 3. Ascarids, flukes and coccidia were more prevalent in pups than in mature dogs, whereas hookworms were detected less often in young dogs than in older ones.