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      • KCI등재
      • KCI등재

        세균성 설사 원인균의 신속검출을 위한 다중 중합효소연쇄반응 검사의 대변검체에 대한 직접 적용

        김미나,조민철,노신애,김경모 대한임상미생물학회 2010 Annals of clinical microbiology Vol.13 No.4

        Background: Causative bacterial agents of infectious diarrheal disease were traditionally diagnosed by stool cultures. Stool culture, however, has a problem because of relatively low sensitivity and long turnaround time. In this study, we evaluated multiplex PCR applied on stool specimens directly to diagnose enteropathogenic bacteria. Methods: From June to September 2009, 173 diarrheal stools submitted for stool cultures were tested by SeeplexⓇ Diarrhea ACE Detection kit (Seegene,Korea) to detect 10 enteropathogenic bacteria. Specimens were cultured for Salmonella, Shigella, Vibrio,and Yersinia. Late 50 specimens were also cultured for Campylobacter. The specimens positive for verotoxin-producing Escherichia coli (VTEC) were further subcultured for detecting enterohaemorrhagic Escherichia coli O157:H7. Electronic medical records were reviewed for clinical and laboratory findings. Results: Of 173 specimens, multiplex PCR and cultures identified enteropathogens in 36 (20.8%) and 8 specimens (4.6%), respectively. While multiplex PCR detected 5 Salmonella, 15 Campylobacter, 1Vibrio, 4 Clostridium difficiles toxin B, 5 Clostridium perfringens, 1 Yersinia enterocolitica, 5 Aeromonas,and 2 VTEC, cultures detected 5 Salmonella, 1Vibrio, 1 Y. enterocolitica, 1 Aeromonas, and 2 E. coli O157:H7. Conclusion: Multiplex PCR would be useful to detect Campylobacter, VTEC and C. perfringens, as well as have equivalent sensitivity to conventional culture for ordinary enteropathogens such as Salmonella,Shigella, Vibrio, Y. enterocolitica. Direct application of multiplex PCR combined with conventional cultures on stool warrants remarkable improvement of sensitivity to diagnose enteropathogenic bacteria. (Korean J Clin Microbiol 2010;13:162-168)

      • KCI등재

        다중 PCR법으로 진단한 Verotoxin 생성 Escherichia coli O157:H7 출혈성 장염인 영아 1예

        김미나,조해선,조민철,노신애,김경모 대한임상미생물학회 2010 Annals of clinical microbiology Vol.13 No.2

        Enterohemorrhagic Escherichia coli (EHEC) is an important cause of bloody diarrhea in children, but is considered to be rare in infants. Herein, a case of infant hemorrhagic colitis of verotoxin-producing E. coli O157:H7 diagnosed by multiplex PCR is reported. A nine-month-old boy was admitted to our hospital with bloody diarrhea for the previous two days. Multiplex PCR using SeeplexⓇ Diarrhea ACE Detection Kit (Seegene, Seoul, Korea) was directly applied to the stool specimens. Amplified bands specific for verotoxin,O157, and H7 indicated the presence of O157:H7 EHEC. The stool specimens were inoculated on sorbitol-MacConkey agar (SMA) and tryptic soy broth containing mitomycin C (TSB-M). Colorless colonies on sorbitol-MacConkey agar were O157-positive. TSB-M enrichment cultures of the stool specimen and the isolates were positive for verotoxin according to an enzyme immunoassay (EIA). The prepared ingredients of baby foods for the patient including ground meat, chopped carrot, chopped cabbage, and white rice porridge showed no EHEC on TSB-M and SMA. The patient’s parents and three-year-old sister did not recently have any gastrointestinal symptoms. Cefdinir was administered for one day and was ceased after diagnosis of EHEC colitis. The stool culture and verotoxin assay were negative on the second day of hospitalization. Application of multiplex PCR and verotoxin EIA directly to diarrheal stool warrants the rapid diagnosis and appropriate treatment of EHEC colitis. (Korean J Clin Microbiol 2010;13:85-89)

      • KCI등재

        흑보찰 분말 첨가에 따른 쿠키의 품질특성 및 항산화 활성

        김미나,이지현,남진경,장혜원 한국식품과학회 2023 한국식품과학회지 Vol.55 No.6

        In this study, the quality characteristics and antioxidant activity of cookies supplemented with the ‘Heukbochal’ barley powder were assessed according to the quantity of barley powder used (0, 15, 30, 45 and 60%). As the barley powder content in the cookies increased, there were decreases in pH value, hardness, spread factor, and loss rate, along with an increase in density and water content. The antioxidant capacities of 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) radicals also increased with higher barley powder content. In the consumer preference test, the sample supplemented with 45% barley powder received the highest scores for color, flavor, texture, and overall preference. Taking both consumer preference and antioxidant properties into consideration, the optimal mixture ratio was determined to be 45% barley powder in cookies.

      • KCI등재

        구독경제 유형과 제품 유형 차이가 구독 의도에 미치는 영향

        김미나,박설우 한국유통학회 2023 流通硏究 Vol.28 No.1

        Purpose: While most of the studies on subscription economy focus on online media streaming services, consumer goods subscription services are already actively being implemented in the market. In this study, the type of subscription service (replenishment vs. curated) and product type (hedonic vs. utilitarian) examined the effect of subscription intention in the consumer goods subscription economy. Furthermore, based on previous studies, the factors mediating this relationship were classified into shopping convenience and perceived enjoyment, and the relationship was examined. Research design, data, and methodology: Consumers’ opinions on consumer products subscription services were collected through the pretest, and based on this, advertisement stimuli for drugstore’s Health & Beauty subscription service and Super supermarkets’ Food & Groceries subscription were produced. Subsequently, an experiment was conducted on 822 randomly recruited people through Amazon MTurk. Experiment 2 (Subscription service type: Replenishment subscription vs. Curated subscription) × 2 (product type: Hedonic goods vs. Utilitarian goods) was designed between groups, and experimental participants were exposed to advertising stimulation of subscription services were asked to respond to the measurement questions of Subscription intention, Shopping convenience, and Perceived enjoyment. Results: As a result of the experiment, the interaction effect of the subscription service types and product types on the subscription intention was confirmed. In addition, through PROCESS macro, the partial mediating effect of shopping convenience was proved in the relationship between subscription service types and subscription intention, and the complete mediating effect of perceived enjoyment was found in the relationship between product type and subscription intention. Conclusions: The study provides the following academic and practical implications. First, for the performance of product subscription services, companies need to change the types of subscription services depending on whether the supplied products are hedonic or utilitarian products. Second, when the subscription service type is Replenishment subscription, it is necessary to focus on the convenience for consumers, and when the product type is hedonic, it is important to improve customers’ enjoyment of shopping. Third, this study has verified the role of factors dealt with in existing shopping studies through experimental studies on subscription services, which are attracting attention as a new trend following the sharing economy. Finally, the influence of perceived enjoyment and shopping convenience, which have been studied as important parameters or regulatory variables related to shopping, was expanded to consumer goods subscription services.

      • KCI등재후보

        뇌사기증자 신장 분배제도의 현황

        김미나,김세란,이수형,오창권,방준배 대한이식학회 2017 Korean Journal of Transplantation Vol.31 No.3

        Background: This study was conducted to analyze the current system for allocation of deceased donor kidney transplantation in Korea, which includes an incentive regulation for candidates registered at the Hospital-based Organ Procurement Organization (HOPO). Methods: Between January 2011 and November 2016, there were 2,655 deceased donors in Korea. During the same period, there were 21,247 current candidates and recipients of kidney, pancreas and simultaneous pancreas-kidney transplants. We analyzed data from all of these donors, candidates, and recipients. Results: Mean waiting times for organ allocation of each priority differed significantly (2nd priority group, 1,701±974 days; 3rd priority group, 1,316±927 days; 4th priority group, 2,077±1,207 days). Additionally, HOPO candidates/deceased donor ratios were very different from each other (maximum, 49; minimum, 0.6). The number of deceased donors in region 1, 2, and 3 were 1,623, 429, and 603, respectively, while the number of transplantations in each region was 3,095, 597, and 1,165, respectively. The candidates registered at region 1 HOPO moved the longest distances on average for transplantation, and this value differed significantly different from that of other regions (56.18±91.9 km vs. 24.66±28.0 km vs. 26.20±37.3 km, P<0.05). Conclusions: The incentive system of current allocation system for deceased donor kidney in Korea does not coincide with the purpose of the ‘Declaration of Istanbul’ and unnecessary social costs have occurred. Therefore, we should make an effort to change our current allocation system to the geographic sequence of organ allocation system.

      • KCI등재

        Causes and Clinical Relevance of Inconclusive SARS-CoV-2 Real-Time Reverse Transcription- PCR Test Results

        김미나,김아람,허규화,성흥섭 대한임상미생물학회 2020 Annals of clinical microbiology Vol.23 No.4

        Background: Inconclusive SARS-CoV-2 real-time reverse transcription-PCR (rRT-PCR) test results, which are positive for one or more target genes but not all, are problematic in clinical laboratories. In this study, we aimed to investigate the cause and clinical relevance of such inconclusive results. Methods: rRT-PCR was performed using the Allplex 2019-nCoV assay kit (Seegene Inc., Korea) targeting the following three genes: E, RdRp, and N. For all inconclusive test results reported from March to June 2020, the frequency per kit, lot number, specimen type, cycle threshold (Ct) and peak values of the amplification curves, positive target genes, and results of repeated or consecutive tests were analyzed. Results: A total of 43,268 tests were conducted, of which 93 (0.21%) were inconclusive—49 from 11 coronavirus disease 2019 (COVID-19) patients and 44 from non-COVID-19 patients. In COVID-19 patients, the results were inconclusive 11.9 ± 4.7 days after diagnosis and were negative 8.8 ± 5.5 days after the inconclusive results were reported. However, in non- COVID-19 patients, they were all negative upon retest and 81.8% of them were identified to have yielded in 2 out of 8 lots. The most frequently positive target genes were N (55.4%) in COVID-19 and RdRp (61.2%) in non-COVID-19 patients, respectively. No difference was observed in the Ct or peak values of the amplification curves for inconclusive samples between COVID-19 and non-COVID-19 cases. Conclusion: Inconclusive test results should be reported neither positive nor negative. Such results can be reported as inconclusive without retesting in COVID-19 patients; however, they should certainly be confirmed by a retest in non-COVID-19 patients or newly diagnosed cases.

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