PGA2-induced HO-1 attenuates G2M arrest by modulating GADD45α expression

Yun-Jeong Choe,고경원,Hyein Lee,이선영,Byung-Chul Kim,Ho-Shik Kim,Ho-Shik Kim 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.4

Prostaglandin (PG) A2, a cyclopentenone PG, arrested the growth of U2OS cells in the G2M phase. While inducing G2M arrest, PGA2 increased the expression of heme oxygenase-1 (HO-1) at the level of transcription along with the accumulation of ROS and the activation of MAPKs including JNK, p38MAPK, and ERK1/2. Among the MAPKs, the inhibition of p38MAPK by a specific chemical inhibitor SB203580, or by RNA interference, but not JNK or ERK1/2, attenuated the PGA2-induced transcription of HO-1. Nacetylcysteine (NAC), a ROS scavenger, prevented PGA2-induced G2M arrest, p38MAPK activation and transcriptional induction of HO-1. PGA2 also stimulated GADD45α expression at the level of transcription, and the knockdown of GADD45α repressed PGA2- induced G2M arrest. Finally, the knockdown of the HO-1 protein elevated PGA2-induced GADD45α expression as well as G2M arrest. Collectively, these results suggest that PGA2 causes an increase in ROS accumulation which initiates both HO-1 transcription via p38MAPK, and G2M arrest via GADD45α transcription, and HO-1 attenuates G2M arrest by modulating the expression of GADD45α.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

나트륨-수증기 반응에 대한 실험적 연구

정지영,정경채,김병호,김태준,최종현,박남국 한국공업화학회 2002 응용화학 Vol.6 No.2

An experimental study for the investigation of the sodium-steam reaction phenomena was carried out. No significant aggressive explosion phenomena were observed as compared with that of sodium-water reactions. It was also observed that the rate of increase of reaction temperature decreases with increasing initial temperature of liquid sodium and that the temperature of the target depends upon the flow rate of injected steam.

정상교합자와 Ⅲ급 부정교합자의 수완부 골성숙 단계와 치아석회화도에 대한 연구

정병초,양규호 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.2

To investigate the relationship between the calcification stages of mandibular canines and the skeletal maturity stage of the hand-wrist in subjects with normal occlusion and Class Ⅲ malocclusion, hand-wrist radiographs and panoramic radiographs were taken from subjects of normal occlusion(94 males,88 females) and Class Ⅲ malocclusion(75 males,76 females) who had no systemic diseases and no history of orthodontic or prosthodonic treatment. Fishman's method for the skeletal maturity stages of the hand-wrist and Demirijian's method for the calcification stages of mandibular canines were used and analyzed. The results were as follows: 1.In subjects with normal occlusion and Class Ⅲ malocclusion,skeletal maturity of the hand-wrist and calcification of mandibular canines at various ages occured earlier in females than males(P<0.05). 2.Comparing the skeletal maturity stages of the hand-wrist and calcification stages of mandibular canines between subjects with normal occlusion and Class Ⅲ malocclusion,there were no significant differences between the groups. 3.The correlation coefficient between the calcification stages of mandibular canines and the skeletal maturity stages of thehand-wrist in subjects with normal occlusion and Class Ⅲ malocclusion showed a high association(P<0.01). 4.In stage 4 of the skeletal maturity of the hand-wrist,the frequency distribution of calcification G stage among the various calcification stages highest both in normal occlusion and in subjects with Class Ⅲ malocclusion.However,there was no signicant differences in the frequency distribution of calcification stage between the groups.

나트륨-물 반응 사고 중/후반기 거동특성 연구

정지영,어재혁,정경채,김병호,김종만,김태준,박남국 한국공업화학회 2003 응용화학 Vol.7 No.1

Generally, the analysis of a sodium-water reaction (SWR) is classified by two major events. One is the very initial stage of peak pressure and shock wave propagation caused by the reaction itself, and the other is the bulk motion including the mass transfer phase in the quasi-steady state of the reaction period during several second or minute orders after leak initiation. The system dynamic response during a sodium-water reaction event shows very different characteristics between the initial stage and the long-term period. In order to investigate the later phase of a SWR event. the code SELPSTA (Sodium-water reaction Event Later Phase System Transient Analyzer) has been developed and the desing and construction of the test facility has been made.

치면열구전색제의 열구 침투에 primer의 효과

정혜선,최병재,최형준,이제호 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.2

본 연구의 목적은 치면열구전색제 수복시에 상아질 접착제의 primer 도포의 치면열구전색제 열구 침투도 향상 효과를 확인하는 것으로 발거된 영구 구치상에서 통상적으로 치면열구 전색제를 도포한 대조군과 Scotchbond™ Multi-Purpose system과 ?? 2 system의 primer 도포 후에 치면열구전색제를 도포한 실험군들의 열구 기저부까지 전색제 침투 성공률을 비교하여 다음과 같은 결론을 얻었다. 1. Scotchbond™ Multi-Purpose system과 All-Bond 2 system의 primer를 사용한 군의 열구 기저부까지 치면열구전색제 침투 성공률은 primer를 사용하지 않은 대조군보다 높았으나 통계학적으로 유의성 있는 차이는 없었다(p>0.05) 2. 열구 깊이와 폭 및 '깊이/폭' 값은 열구 기저부까지 전색제 침투성공율에 대하여 통계학적으로 유의성 있는 효과를 나타내었다.(p<0.05) 3. 열구 기저부까지 전색제 침투 성공률은 열구 깊이가 25㎛ 증가할 때마다 약 0.9배로 감소하였고, 열구 입구의 폭이 25㎛ 증가할 때마다 약 1.1배로 증가하였으며, 열구의 '깊이/폭' 비율이 1 증가할 때마다 약 0.6배로 감소하였다. The objective of this study is to confirm the effect of dentine bonding primer application on penetration of sealant. Extracted permanent molars were used to compare penetration success rate of control group (sealant application only) and experimental groups (sealant application after applying the primers of Scotchbond™ Multi-Purpose system and All-Bond 2 system). The following results were obtained: 1. The experimental groups using the primers showed increased sealant penetration success rate to the base of fissure when compared to control group but there was no statistically significant differences(p>0.05). 2. The depth, width and 'depth/width' value of fissure had statistically significant effect on sealant penetration success rate(p<0.05). 3. The penetration success rate decreased about 0.9 times as the depth of fissure increased every 25㎛, and increased about 1.1 times as the width of the fissure orifice increased every 25㎛, and decreased about 0.6 times as the 'depth/width' value increased every 1. From the above results, it can be concluded that fissure morphology had a great effect on sealant penetration and for better penetration, use of dentine bonding primer can be helpful but it needs more study in clinical bases.

모세관 전기영동 장치를 이용한 음이온계 계면활성제의 분석

정혁,김승선,이병민,강호철,이원,김해동 숙명여자대학교 환경과학연구소 1995 환경과학 Vol.2 No.-

모세관 전기영동 장치를 이용하여 금속 표면 세척제(상품명 BFA와 BCA) 2종에 포함된 음이온 계면활성제 대한 정성 및 정량분석을 수행하였다. Acetonitrile과 sodium benzoate를 포함하는 pH 10의 완충용액을 이용하여 18kV의 전압하에서 분리한 결과10^4 이상의 이론단수를 보이는 좋은 electropherogram을 얻을 수 있었다. S/N∼3인 농도를 기준으로 검출한계는 ∼5ppm이었으며 표준물질을 이용하여 얻은 검정곡선의 경우 농도가 10∼100ppm의 범위에서 ∼0.99 이상의 상관계수를 갖는 좋은 직선성을 보였으며, 이러한 결과를 바탕으로 분석한 결과 BFA의 경우 음이온 계면활성제의 각 성분은 octanoate, decanoate, dodecanoate, tetradecanoate, hexadecanoate 등이었으며 상대비는 각각 1.0 : 1.0 : 6.5 : 2.1 : 0.8 이었다. Qualitative and quantitative analysis for the anionic surfactants used in the metal washing fluid (brand names are BFA and BCA) was performed by the capillary electroihoresis. Acetonitrile and sodium benzoate were mixed with the buffer solutioin which controlled at pH 10. Under the 18kV applied voltage, the electropherograms have shown the theoretical plates more that 10^4. Determined as the concentration at the S/N∼3, the typical detection limit was ∼5ppm and the calibration curves have shown the correlation coefficients higher than ∼0.99. Based on these results, it was concluded that each components were octanoate, decanoate, dodecanoate, tetradecanoate, hexadecanoate and the relative ratio was 1.0 : 1.0 : 6.5 : 2.1 : 0.8 for the BFA.

정상교합자와 Ⅲ급 부정교합자의 수완부 골성숙 단계와 치아석회화도에 대한 연구

정병초,양규호 전남대학교 치과대학 1995 전남치대논문집 Vol.7 No.1

To investigate the relationship between the calcification stages of mandibular canines and the skeletal maturity stage of the hand-wrist in subjects with normal occlusion and Class Ⅲ malocclusion, hand-wrist radiographs and panoramic radiographs were taken from subjects of normal occlusions(94 males, 88 females) and Class Ⅲ malocclusions(75 males, 76 females) who had no systemic diseases and no history of orthodontic or prosthodontic treatment. Fishman's method for the skeletal maturity stages of the hand-wrist and Demirijian's method for the calcification stages of mandibular canines were used and analysed. The results were as follows : 1. In subjects with normal occlusion and Class Ⅲ malocclustion, skeletal maturity of the hand-wrist and calcification of mandibular canines at various ages occured earlier in females than in males(p<0.05). 2. comparing the skeletal maturity stages of the hand-wrist and the clacification stages of mandibular canines between subjects with normal occlusion and Class Ⅲ malocclusion, there were no significant differences between the groups. 3. The correlation coefficient between the calcification stages of mandibular canines and the skeletal maturity stages of the hand-wrist in subjects with normal occlusion and Class Ⅲ malocclusion showed a high association(p<0.01). 4. In stage 4 of the skeletal maturity of the hand-wrist, the frequency distribution of calcification G stage among the various calcification stages was highest both in nomal occlusion and in subjects with Class Ⅲ malocclusion. However, there was no significant difference in the frequency distribution of calcification stages between the groups.

양식장에서 사용되는 수질정화제용 종균제의 효용성 평가

정병곤,김병효,장호영 7개 국립대학교 환경연구논문집 공동발행 위원회 2005 공업기술연구 Vol.5 No.-

Laboratory experiments were conducted to evaluate the effectiveness of commercial bioaugumentation agent in aquaculture farm. 4 dIfferent domestic commercial product and 2 different imported product were used in experiment. Batch reactor was runned under different condition of substrate concentration and agent dosing rate. In view of experimental results obtained in this experiment, nitrification efficiencies of domestic products are superior to those of imported product. When the economic evaluation has been done in view of purchasing cost, imported agent is superior to domestic one. When considering cost and quality simultaneously, cost discrepancy between products is quite serious especially in domestic product. Therefore, it is nessary to establish proper guideline in choosing bioaugumentation agent in aquaculture farm. In view of farmer, caution should be required in selecting adequate angent in aquaculture farm.

소듐-물 반응사고 중/후반기 계통거동특성 연구

정지영,어재혁,김태준,정경체,김병호,한도희,박남국 한국공업화학회 2004 응용화학 Vol.8 No.1

In order to investigate the later phase of a sodium-water reaction (SWR) event in KALIMER (Korea Advanced Liquid MEtal Reactor), the code SELPSTA (Sodium-water reaction Event Later Phase System Transient Analyzer) has been developed and an experimeantal study has been carried out for verification of the simple analysis model applied to the code. The 24 data set obtained in the experiment have been pre-analyzed. Comparison of SELPSTA results with experimental data shows fairly good agreement in the transient.