Sulforaphane protects against acetaminophen-induced hepatotoxicity

Noh, Jung-Ran,Kim, Yong-Hoon,Hwang, Jung Hwan,Choi, Dong-Hee,Kim, Kyoung-Shim,Oh, Won-Keun,Lee, Chul-Ho Elsevier 2015 Food and chemical toxicology Vol.80 No.-

<P><B>Abstract</B></P> <P>Oxidative stress is closely associated with acetaminophen (APAP)-induced toxicity. Heme oxygenase-1 (HO-1), an antioxidant defense enzyme, has been shown to protect against oxidant-induced tissue injury. This study investigated whether sulforaphane (SFN), as a HO-1 inducer, plays a protective role against APAP hepatotoxicity <I>in vitro</I> and <I>in vivo</I>. Pretreatment of primary hepatocyte with SFN induced nuclear factor E2-factor related factor (Nrf2) target gene expression, especially HO-1 mRNA and protein expression, and suppressed APAP-induced glutathione (GSH) depletion and lipid peroxidation, which eventually leads to hepatocyte cell death. A comparable effect was observed in mice treated with APAP. Mice were treated with 300 mg/kg APAP 30 min after SFN (5 mg/kg) administration and were then sacrificed after 6 h. APAP alone caused severe liver injuries as characterized by increased plasma AST and ALT levels, GSH depletion, apoptosis, and 4-hydroxynonenal (4-HNE) formations. This APAP-induced liver damage was significantly attenuated by pretreatment with SFN. Furthermore, while hepatic reactive oxygen species (ROS) levels were increased by APAP exposure, pretreatment with SFN completely blocked ROS formation. These results suggest that SFN plays a protective role against APAP-mediated hepatotoxicity through antioxidant effects mediated by HO-1 induction. SFN has preventive action in oxidative stress-mediated liver injury.</P> <P><B>Highlights</B></P> <P> <UL> <LI> SFN pretreatment increases the cell viability against APAP-induced toxicity. </LI> <LI> SFN pretreatment protects depletion of cellular GSH after APAP treatment. </LI> <LI> SFN pretreatment enhances Nrf2 target gene expression, especially HO-1 after APAP treatment. </LI> <LI> SFN has protective effect against APAP overdose-induced liver injury <I>in vivo</I> model as well. </LI> </UL> </P>

위암 환자의 복강내 투여를 위한 Activated Charcoal-Alginate Bead 제형으로부터 Mitomycin C의 용출 거동

이진호,최선웅,서중기,김동민,정경수,오정연,김진향,노승무,민병무,김용백,김창식,박근성,강대영,송규상,양준묵,조준식,정현용,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Locoregional recurrence is the most common type of recurrence in surgical operation of gastric adenocarcinoma, and peritoneal dissemination is one of the most difficult problems in advanced gastric adenocarcinoma treatment. Because the peritoneal cavity is the most common site of the first recurrence after gastric cancer resection, intraperitoneal chemotherapy seems a logical choice for cancer chemotherapy. In this study, Mitomycin C (MMC)-activated charcoal (CH)-alginate (ALG) beads were prepared by the mixtures of CH particles adsorbed with MMC as an anti-cancer drug and aqueous alginate solution. The alginate is recognized as biodegradable, nontoxic, and biocompatible. The release of MMC from the beads in 0.1 M Tris buffer was stable and continuous until about 1 week. The MMC-CH-ALG beads can be applied in the peritoneal cavity for intraperitoneal chemotherapy since they provide a good adhesiveness on the tissue and controlled release pattern of the drugs.

학교폭력 피해 청소년의 정신의학적 후유증에 관한 사례 연구

송동호,육기환,이호분,노경선 대한소아ㆍ청소년정신의학회 1997 소아청소년정신의학 Vol.8 No.2

전반적발달장애 아동 및 발달성언어장애 아동의 뇌 단일광자방출전산화단층촬영 소견에 대한 예비연구

이호분,신의진,육기환,유영훈,노경선,송동호 대한소아ㆍ청소년정신의학회 1997 소아청소년정신의학 Vol.8 No.2

단편 소설의 낱말 교육 자료 연구 : 「논 이야기」외 3편의 낱말을 중심으로

이희중,노일환,최명엽,신동일,이준호 전주대학교 교육문제연구소 2001 敎育論叢 Vol.16 No.-

선택적 함구증 환자에서의 Fluoxetine 치험

송동호,박민숙,남수용,육기환,노경선,이홍식 대한소아ㆍ청소년정신의학회 1997 소아청소년정신의학 Vol.8 No.2

과학 영재를 위한 캠프 운영과 교육 프로그램의 개발 준거

여상인,노석구,강호감,이희순,홍석인,송상헌,정동권 인천교육대학교 과학교육연구소 2003 과학교육논총 Vol.15 No.-

본 연구는 영재 프로그램과 그 운영의 적절성과 타당성을 판단하는데 기초 자료가 될 수 있는 준거를 추출하기 위한 연구이다. 과학캠프 활동 프로그램에 대한 학생들의 흥미와 유익성, 영재캠프 운영에 관련된 문항으로 구성된 설문지를 캠프에 참가한 학생과 그 학부모에게 실시하여 분석하였다. 영재 캠프에 참가한 학생은 직접 참여할 수 있으면서 지적 호기심을 자극하는 활동에 흥미를 느꼈고, 놀이 요소가 포함된 프로그램을 많이 선호하였다. 학생들이 모르거나, 중요하다고 생각하거나, 일상생활에 적용할 수 있는 과학 지식이나 원리를 배웠다고 인식하는 경우, 유익한 프로그램으로 인식하였다. 과학 영재 캠프의 운영에 있어서 많은 학생이 방학 중에 1주일 정도의 기간으로 합숙하는 프로그램 운영 방식을 선호하였다. 이러한 연구 결과를 토대로 본 연구에서는 캠프 활동 프로그램과 운영에 대한 몇 가지 시사점을 제안하였다. This study is to find out criteria and basic data to evaluate effectiveness and validity of program for gifted children in science. To this purpose, a questionnaire to investigate the perception of interest and usefulness for camp activities was administered to the gifted children registered to science camp. By analyzing their responses, we could conclude that the gifted children thought the activities, which stimulating their curiosity or providing opportunities to participate, to be interesting to them. They preferred the activities like a play or a game and considered that a useful activities should have something worth-learning. They and their parents preferred to stay in a camp for a week. Based on this research, we suggested some implications about academic camp program for gifted children in science.

cDNA array 방법을 이용한 망간에 노출된 흰쥐 뇌기저핵의 유전자발현 분석

이채관,노성민,문덕환,,김정호,손병철,김대환,이창희,김휘동,김정원,김종은,안진홍,이채언 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Objectives: This study investigated the gene expression profile in basal ganglia of manganese-exposed rats based on cDNA array analysis. Methods: For cDNA array, 25 male Sprague-Dawley rats (250±25 g) were intraperitoneally injected with 25 ㎎/㎏ B. W./day of MnCl2 (0.3 ㎖) for 10 days. For dose-related gene expression analysis, rats were intraperitoneally injected with 0.2, 1.0, and 5.0 ㎎/㎏ B. W/day of MnCl2 for 10 days. Control rats were injected with an equal volume of saline. RNA samples were extracted from brain tissue and reverse-transcribed in the presence of [α^(32)P]-dATP. Membrane sets of the Atlas Rat 1.2 array Ⅱ and Toxicology array 1.2 kit (Clontech, Palo Alto, CA) were hybridized with cDNA probe sets. Northern blot hybridization method was employed to assess the dose-related gene expression. Results: Fifty-two genes showed significant changes in expression of more than two-fold. Twenty-eight were up-regulated and 24 were down-regulated in the manganese-exposed group compared to the control. Among the 52 genes, 28 genes including nuclear factor I-X1 (NF1-X1), neuroligin 2 and 3, mitochondrial stress-70 protein (MTHSP70), neurodegeneration-associated protein 1 (Neurodap 1), multidrug resistance protein (MDR), and endoplasmic reticulum stress protein 72 (ERP72), were reported for the first time related to the manganese-induced neurotoxic-metabolism in the rat basal ganglia. According to the dose-related gene expression analyses, MTHSP70, Neurodap 1 and ERP72 genes were up-regulated compared to the control even in the group exposed to low manganese dose ( 0.2 ㎎/㎏ B.W./day). Conclusions: Twenty-eight genes detected for the first time in this study were closely related to the manganese-induced neurotoxic-metabolism in the rat basal ganglia and further study of these genes can give some more useful information about the manganese metabolism.

인천교육대학교 과학ㆍ수학 영재 캠프의 운영에 관한 기초 연구

송상헌,정동권,강호감,여상인,노석구 인천교육대학교 과학교육연구소 2003 과학교육논총 Vol.15 No.-

경기도와 인천시의 초등교사 양성을 책임지고 있는 인천교육대학교의 입장에서는 경기·인천지역의 초등 과학과 수학분야의 영재교육 프로그램을 경험하려는 학생들에게 기대와 요구를 충족시켜 줄 책임과 의무가 있다. 이에 인천교육대학교에서는 과학교육연구소가 중심이 되어 영재교육연구원이나 영재교육원을 설치하기 위한 장기적인 목표 하에 과학·수학 영재교육을 실시할 수 있는 실제적인 방안을 모색할 필요성에 따라, 우선 실험적으로 캠프 형식의 운영을 실험해 보았다. 본 연구는 이러한 구체적인 현장 사례연구를 바탕으로 인천교육대학교에서 주관하는 영재교육추진 기본 계획 수립을 위한 초등 과학·수학분야 영재교육 프로그램의 운영 모델과 그것의 실천에 필요한 장기적인 전망을 제안한다. 캠프에서 운영할 수 있는 프로그램의 성격은 심화형의 다 주제 탐구형으로 진행하면서 다양한 교과나 체험을 위주로 한 내용과 형식을 갖출 것을 권장한다. 영재캠프에 참가할 수 있는 자격 조건과 선발 기준은 운영하고자 하는 프로그램의 내용과 성격에 가장 부합하도록 특성화되어야 하고, 캠프에 참가한 학생들에 대한 지속적인 사후 관리가 필요하다. 지속적인 교육과 운영을 위해서는 장기적인 연구 계획 및 예산 수립이 필요하며, 각 교과의 전문가들과 보다 장기적인 공동연구를 수행해야만 한다. 그러면서 영재교육연구원이나 영재교육원의 운영을 목표로 영재교사 양성을 위한 직무연수 프로그램이나 부설 사회교육원의 프로그램을 통해 학부모 교실을 운영할 수 있음도 제안한다. This is a basic study on the science and math camp program for the gifted and talented children in science and mathematics. We organized and managed empirical camp twice for 2 years. From those experiences, we proposed a sample model to manage good camp and a long term vision. This is the result of the basic study on the management of the science and mathematics camp program in Inchon National University of Education.

경피적 자궁동맥 색전술로 치료한 산후 출혈 1례

정명철,김호성,김경철,노지현,고재환,김용봉,권동일 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Postpartum hemorrhage remains one of the leading causes of maternal deaths. The major causes of postpartum hemorrhage include uterine atony, retained placental fragment, placenta accreta, and lower genital tract lacerations. Conservative treatment is based on administration of uterotonic drugs, vaginal packing, and surgical repair of genital tract lacerations. When bleeding fails to respond to conservative treatment, surgical ligation of uterine vessels or hemostatic hysterectomy is performed. We present a case of postpartum bleeding treated by selective uterine arterial embolization with a brief review of previous literatures of postpartum hemorrhage & uterine arterial embolization.