DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

해상형 국립공원 탐방수요의 요인분해

모수원 ( Mo Soo-won ),이광배 ( Lee Kwang-bae ),박정환 ( Park Jeong-hwan ) 한국도서(섬)학회 2018 韓國島嶼硏究 Vol.30 No.2

국내관광은 국제관광의 변동성이 주는 충격을 완화하려 관광산업의 안정성을 담보하는 역할을 수행함에도 불구하고 국내관광부문은 활성화되지 못하고 있다. 이러한 점은 우리나라의 해외관광객은 2010년-2017년에 연평균 11.3%라는 높은 증가를 보인데 비해 우리나라 국립공원의 탐방객은 연평균 1.5% 성장이라는 초라한 실적을 드러내고 있다는 데서 알 수 있다. 더욱 산악형 국립공원에 대해서는 비교적 많은 연구가 이루어지고 있는데 비해 해상형 국립공원에 대해서는 상대적으로 연구와 관심이 빈약한 편이다. 이에 본고는 변이할당분석과 BCG분석을 이용하여 해상형 국립공원 탐방객의 변동요인을 식별한다. 먼저 변이할당분석을 적용하여 4개 해상형 국립공원 탐방객의 변동을 국립공원 전체에 대한 탐방수요가 개별 국립공원탐방수요에 미치는 국립공원효과, 국립공원 전체에 대한 매력과 해상형 국립공원에 대한 매력의 차이가 개별 국립공원 탐방수요에 미치는 해상형 국립공원효과, 그리고 개별 국립공원과 해상형 국립공원의 탐방수요가 미치는 소위 경쟁력효과와 같은 3개 요인으로 분해한다. 또한 변이할당분석을 정태적 분석과 동태적 분석으로 구분하여 정태적 분석과 동태적 분석의 3개효과가 부호와 크기에서 상당한 차이를 노정한다는 것도 밝힌다. 구체적으로 우리나라 4개 해상형 국립공원의 탐방객 변동을 요인별로 분해하여, 정태적 분석에서는 국가효과와 구성효과가 양의 값으로 4개 해상형 국립공원 탐방객을 증가시키나, 동태적 분석에서는 구성효과의 경우 2013년부터, 국가효과의 경우 2014년부터 음의 부호를 갖는다는 것을 밝힌다. 탐방객이 증가한 오동도, 다도해해상국립공원, 태안해안국립공원은 물론 탐방객이 감소한 변산반도국립공원에서도 개별 국립공원의 경쟁력을 의미하는 지역효과가 가장 큰 영향을 미친다는 것도 보인다. BCG 매트릭스를 적용하여 오동도와 다도해해상국립공원은 star 영역으로 상승한 후star 위상을 유지하며, 변산반도국립공원은 star에서 cash cow와 child로 위상이 변화하여 탐방객 증가가 평균 이상을 유지하고 있는데 비해 태안해안국립공원은 child에서 dog로 추락함을 보인다. 이러한 분석을 통해 4개의 해상형 국립공원 모두에서 지역효과가 탐방객 변동에 가장 큰 영향을 미친다는 것과, 오동도와 다도해해상국립공원의 경쟁력은 우수하나 변산반도 국립공원의 경쟁력은 정체상태에 있으며, 태안해안국립공원의 경쟁력은 하락하고 있음을 밝힌다. BCG분석에서는 오동도와 다도해해상국립공원이 탐방객에서 경쟁력이 있다는 것과 태안해안국립공원에서 가장 큰 경쟁력 향상이 이루어져야 한다는 것도 밝힌다. Tourism is widely considered one of the world's largest and rapidly growing industries. Domestic tourism, however, remains an under-researched theme in many countries involving Korea partly due to the difficulty to track domestic tourism and with governments and policy-makers placing emphasis on the attractive higher expenditures of foreign visitors. It is stated that a vibrant domestic tourism sector can cushion the industry from fluctuations of the international tourism market and bring stability and predictability in the industry. Nature-based tourism to national parks gives us broad benefits such as improved physical and mental health, stress reduction and increased social cohesion. Employment is one of tourism’s main development advantages and its role in promoting economic opportunities for communities adjacent to national parks has long been appreciated. But tourism performance in Korean national parks continues to dwindle and stagger. This paper introduces a shift-share decomposition to break down the tourism to the marine national parks into three components: one measuring national effect, and two other components due to changes in compositional effect and regional effect respectively. While the static shift-share analysis shows that the growth in marine national parks’tourism seems to be due to positive national park effect as well as marine national park effect, the dynamic regional effect appears to have a negative effect on visitors. This paper also employs the positioning analysis on national parks using Boston Consulting Group matrix over the period 2011-2016. The BCG analysis shows that Odongdo and Dadohaehaesang succeed in rising to a star area, Byeonsanbando ranges over a cash cow to a child, but Taeanhaean degrades from a child to a dog area.

한국 국립공원 산림생태계의 수목 탄소저장량 평가

이상진,박홍철,박관수,김현숙,이창민,김진원,심규원,최승운,Lee, Sang-Jin,Park, Hong-Chul,Park, Gwan-Soo,Kim, Hyoun-Sook,Lee, Chang-Min,Kim, Jin-Won,Sim, Gyu-Won,Choi, Seung-Woon 한국환경복원기술학회 2022 한국환경복원기술학회지 Vol.25 No.3

The purpose of this study is to quantitatively evaluate the amount of carbon storage for trees in forest ecosystem to support the foundation for carbon neutrality implementation in Korea National Park. It targeted 22 national parks designated and managed as national parks in Korea, and conducted research on forest trees in the terrestrial ecosystem among various natural and ecological carbon sink. The survey and analysis method followed the IPCC guidelines and the National Greenhouse Gas Inventory in Korea. The amount of tree carbon storage in the forest ecosystem of Korea National Park was confirmed to be about 218,505 thousand CO₂-ton and the amount of carbon storage per unit area was 570.8 CO₂-ton per hectare. Compared to 299.7 CO₂-ton per hectare, the average carbon storage per unit area of the entire Korean forest, it was found that about twice as much carbon was stored when assuming the same area. In other words, it means that the tree carbon storage function of the national park is about twice as high as that of the average tree carbon storage function of entire Korean forest. It has great implications in Korea National Park not only provides biodiversity promotion and exploration services as a national protected area, but also performs excellent functions as a carbon sink.

A Single-slope ADC with Digitally Programmable Dynamic Range for CMOS Image Sensor

Soo?Yang Park,Hee-Gyeong Yang,Sang?Hee Son,Won Sup Chung 대한전자공학회 2004 ITC-CSCC :International Technical Conference on Ci Vol.2004 No.7

콜롬비아 소가모스에서 미세먼지 감축 시나리오 분석

박일수(Park, Il-Soo),장유운(Jang, Yu-Woon),하상섭(Ha, Sang-Sub),장수환(Jang, Su-Hwan),정경원(Chung, Kyung-Won),김혜원(Kim, Hye-Won),김희선(Kim, Hee-Sun) 한국외국어대학교 중남미연구소 2016 중남미연구 Vol.35 No.2

콜롬비아 소가모스 지역에서 미세먼지 감축 시나리오를 TAPM 모델을 활용하여 도출하고 평가하였다. 시나리오 1의 제철소 및 금속 등 중공업 오염원을 대상으로 미세먼지 방지 기술을 이용하여 배출량을 80% 감축하는 방안에서는 도시 중심 평균 농도는 6.5 μg m-3로 1.5 % 감소하였고, 모델영역에서 최고 평균농도는 387 μg m-3 로 변동 없었다. 시나리오 2의 벽돌 및 석회공장 등을 대상으로 연료를 석탄에서 코크스로 교체하여 90 % 감축하는 방안에서는 도시 중심 평균 농도는 4.5 μg m-3로 31.8%, 모델영역에서 최고 평균농도는 111 μg m-3 로 71.6 % 감소하였다. 시나리오 1과 시나리오 2를 결합한 시나리오 3에서는 도시 중심에서 평균 농도는 4.3 μg m-3로 34.8 %, 모델영역에서 최고 평균농도는 110 μg m-3 로 71.7 % 감소하였다. 소가모스 지역에서 대기환경을 개선하는 로드맵 수립에는 수공업에서 주로 사용되는 석탄 연료를 청정연료인 코크스로 교체하는 방안이 최우선으로 고려되어야 하겠다. 이후 미세먼지 방지 시설을 굴뚝에 설치하는 방안도 차선책으로 고려하여 환경과 경제가 선 순환하는 녹색성장관점에서 장기적인 미세먼지 개선 마스터 플랜이 수립되어야 하겠다. Reduction scenarios for PM10 concentration in Sogamoso, Colombia were analyzed using the TAPM( The Air Pollution Model). The average concentration of PM10 in the downtown area was 6.5 μg m-3, a reduction of 1.5 %. The maximum average concentration in the model domain was 387 μg m-3 without any reduction in scenarios 1 which the PM10 emissions from heavy industries such as iron and metal was reduced by 80 % using the prevention technology. In scenario 2 which the PM10 emissions from the brick and lime industries was reduced by 90 % substituting coke (a cleaner fuel) for coal, the average concentration of PM10 in the downtown area was 4.5 μg m-3, a reduction of 31.8 % , the maximum average concentration in the model domain was 111 μg m-3 , a reduction of 71.6 %. In scenario 3 which the scenarios 1 and 2 were combined, the average concentration of PM10 in the downtown area was 4.3 μg m-3, a reduction of 34.8 % and the maximum average concentration was 110 μg m-3, a reduction of 71.7 %. To put forward a road map for the reduction of PM10 in the Sogamos region, substituting coke for coal in manual industries should have priority over reduction policies. Also, it is highly recommended that a longterm master plan for reducing PM10 concentrations should be established with a focus on environmentally-friendly development and future green growth policy with a win-win relationship between economy and environment.

주의력결핍과잉행동장애와 Alpha-1C-Adrenergic 수용체 유전자와의 연관성에 대한 연구

조수철,김재원,김붕년,황준원,박미라,김순애,조대연,유희정,정운선,손정우,박태원 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.1

Objectives : Neurobiological and pharmacological research has suggested that the dysregulation of the central noradrenergic systems might be involved in the pathophysiology of ADHD. The aim of this study was to examine the association of the alpha-1C-adrenergic receptor gene (ADRA1C) with ADHD in Korean subjects. Methods : In a case-control study, we assessed 186 DSM-IV ADHD probands and 150 normal controls. One hundred and eight trios were studied in a family-based association analysis. Psychiatric diagnoses were derived through structured diagnostic interviews. For the clinical evaluation of the ADHD subjects, the Child Behavior Checklist (CBCL), the ADHD Rating Scale-IV (ARS) and the Junior Temperament and Character Inventory (JTCI) were administered. A computerized continuous performance test (CPT) was used to measure the inattention and impulsivity of the ADHD children. Results : There were no significant differences in the genotype or allele frequencies of the ADRA1C PstI polymorphism between the ADHD and control group (p>0.05). The transmission disequilibrium test (TDT) analysis observed no evidence for biased transmission of any of the alleles of the PstI polymorphism. There were no significant differences in the CPT or JTCI profiles between those ADHD subjects with the CC genotype and those with the other (CT+TT) genotypes at the PstI polymorphism. Conclusion : The results of this study do not support the ADRA1C as a major genetic susceptibility factor in ADHD.

완화환율의 장기구매력평가 : 정수차분과 분수차분

모수원(Soo Won Mo),박석호(Seok Ho Park) 한국국제경영학회 1999 國際經營硏究 Vol.10 No.1

구매력평가(PPP)는 국제금융이론에서 중요한 비중을 차지하고 있다. 초기 환율의 화폐적 모형인 신축물가모형은 지속적인 구매력평가의 성립을 전제로 하고 있으며, 경직물가모형은 환율의 구매력평가로부터의 단기적 괴리를 허용하나 장기에서는 균형이 이루어짐을 상정하고 있다. 이러한 PPP의 중요성에도 불구하고 단기에서 PPP가 성립하지 않는다는 데에는 일반적으로 견해가 일치하고 있으나, 장기에서의 PPP 타당성에 대해서는 많은 논란이 되고 있다. 이러한 결과는 저자에 따라 물가지수, 자료의 빈도 또는 이용하는 검정기법상의 차이가 있기 때문이다. 일반적으로 장기PPP검정에는 흔히 공적분기법이 주로 이용되고 있다. 그러나 많은 문헌들이 사용하는 정수차분에 근거를 두는 기존의 Dickey-Fuller류의 공적분기법은 충격효과가 즉시 소멸하는가 아니면 영구히 지속되는가 하는 양 극단의 경우만을 다룰 수 있을 뿐이다. 즉 시계열의 특성이 I(0)인가 흑은 I(1)인가를 식별하는 데 그치고 있다. 이에 따라 정상성과 추세회귀성향을 구분할 수 있는 장점을 지닌 분수차분에 의한 검정이 이루어져야 한다. 이러한 분수차분에 의한 GPH검정을 통해 한국의 원화환율은 장기에 있어서 PPP를 지지하지 않음을 밝힌다.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

의원의 특성에 따른 의료보험진료의 차이 연구

서수교,박재원 대구보건대학 1999 대구보건대학 論文集 Vol.19 No.-

The primary medical care is the most important part in the Medical Delivery System. So this study was to survey the average of treatment amount among the kind of specialty through the medical insurance claims concerning 963 clinics during the period of one year, 1997, in Taegu. The average revenue of medical treatment amount was 187,950,000won, the average case of medical treatment amount was 9,255cases and the charges per case was 21,596won. The result of survey about the internists, general surgeons, family physicians, general practitioners and pediatricians was as follows : Upon the treatment cases, significant variables of the internists were the number of practitioners of clinic, employed numbers and the number of equipments. Significant variables of the general surgeons were the age of practitioners, the opening year, the number of practitioners, employed numbers and the number of beds. Significant variables of the family physicians were the age of practitioners, the opening year and the number of equipments. Significant variables of the general practitioners were the number of practitioners of clinic and number of beds. Significant variables of pediatricians were the number of practitioners and employed numbers. In view of the tendency for the park of medical insurance to enlarge more and more in revenue of the whole treatment amount, and through the analysis of factors influencing upon treatment amount of medical insurance, we will anticipate the expenditure of treatment amount of insurance and need to establish efficient medical insurance policy to reduce the differences between the distributions of treatment amount of insurance among special departments. Therefore the health manpower policy about the private medical practitioners will be continuously study.

백서와 기니픽의 대뇌피질에서 Opioid Kappa 수용체의 특성에 관한 연구

김기원(Kee-Won Kim),노혜원(Hye-Won Rho),김형일(Hyoung-Il Kim),은재순(Jae-Soon Eun),소수미(Soo-Mi Soh),조규박(Kyu-Park Cho) 대한약리학회 1994 대한약리학잡지 Vol.30 No.2

In this study, we tested the influences of several κ opioid ligands on the [³H]diprenorphine binding in rat and guinea pig cortex membrane preparations. Using paradigm to block μ and δ opioid receptors with DAMGO(1μM) and DPDPE(1μM), [³H]diprenorphine labeled κ sites. Competition analysis in both rat and guinea pig cortex has shown a single population of [³H]diprenorphine binding site with different Kd values, respectively. There is a significant difference in Ki values of (-) WIN44441 and (+)WIN44441 in both rat and guinea pig cortex. Bremazocine, (-)ethylketocyclazocine, (-)cyclazocine, nor-binaltorphimine effectively inhibited the [³H]diprenorphine binding with different Ki values in rat and guinea pig cortex. U-69,593, U-50,488H and dynorphine-A (1-8) did not inhibit the [³H]diprenorphine binding in rat but in guinea pig cortex. Nor-binaltorphimine was a ligand discriminate the κ₁, and κ₂ receptor most effectively. We, also, examined the influence of Na ion and GTPγS, a nonhydrolyzable guanine nucleotide analog, on the inhibition of [³H]diprenorphine binding by diprenorphine, (-)ethyl-ketocyclazocine, U-69,593 and bremazocine. By the replacement of NaCl with N-methy-D-glucamine or addition of GTPγS, Ki values of diprenorpnine were not changed and that of ethylketocyclazocine were changed significantly in both rat and guinea pig cortex. The Ki value of bremazocine was decreased by removal of Na ion, and increased by GTPγS, however, was not changed by any one of either. These results suggest that there are 2 kinds of subtypes of κ opioid receptor, κ₁, and κ₂, showing different Ki values for various κ opioid ligands, also, bremazocine possess the antagonistic property at κ₂ site which is dominant subtype of K receptor in rat cortex.