전국 10개 종합병원 중환자실 환자의 직장내 Vancomycin-resistant Enterococci(VRE) 보균율, 보균의 위험요인 및 유전적 다양성 조사

정희진,송준영,엄중식,김우주,최선주,최정현,이혁,정문현,이광미,신동현,손보라,최종성,우흥정,권정아,이경원,최강원,박승철 대한감염학회 2002 감염 Vol.34 No.5

肺吸蟲症에 있어서 尿를 이용한 免疫學的 診斷에 관한 硏究

李榮喆,李駿商,林漢鍾 고려대학교 의과대학 1986 고려대 의대 잡지 Vol.23 No.3

Human paragonimiasis is one of the most common trematode diseases and of very important public health problems in Korea. Since neither skin test always represents current infection nor recovery of Puragovzinius westermani ova, the immunodiagnosis of human paragonimiasis is not always possible, a more feasible, sensitive and specific method is required. To investigate the feasibility and diagnostic accuracy of using the enzyme-linked immunosorbent assay (ELISA) with a urine sample in the immunodiagnosis of human paragonimiasis. 72 normal control subjects. 100 subjects with skin test positive for paragonimiasis. 19 patients with paragonimiasis. 13 subjects with serum optical density of more than 1.00 for Paragonimus antigen and clonorchiasis subjects were studied. ELISA was performed with Verona! buffered saline antigen of Paragonimus westermani. peroxidase conjugated anti-rabbit IgG and orthophenylene diamine as a substrate by micro-method. The results obtained are as follows: 1. The mean O.D. (optical density) values of ELISA of normal control subjects was 0.19±0.07 in the urine samples and 0.16 & 0.09 in the serum samples. 2. The mean O.D. values in the urine samples of 100 positives by skin test was 0.47±1 0.64 and in the serum samples was 0.65±0.72. The relation of the absorbance values between the urine (x-axis) and serum (y-axis) samples made the regression equation: y = 0.95x+0.206, and showed high correlation (r = 0.847, n = 100, p < 0.01). 3. Pseudopodia showed at the wheal by the skin test in the 12 (63%) out of 19 paragonimiasis. The mean O.D. values of 19 paragonimiasis was 1.41±0.58 in the urine samples and 1.64±0.38 in the serum samples. The O.D. values of confirmed paragocimiasis cases were 0.5 or over in the almost every cases using a urine sample. 4. Six cases of paragonimiasis which were treated effectively showed same reactions of the skin test and the O.D. values in the serum samples of other paragoniniiasis before treatment, but the O.D. values in the urine samples was a tendency of decrease. In conclusion, these findings suggested that ELISA using a urine sample was more feasible and sensitive in the immunodiagnosis of human paragonimiasis in the mass screening.

석면관련 폐질환의 진단에 있어서 흉부고해상도전산화단층촬영검사(HRCT)의 유용성

이현재,손지언,,홍영습,,이영일,예병진,유창훈,정갑열,김상훈,임형준,장은철,김정만,김준연 大韓産業醫學會 2006 대한직업환경의학회지 Vol.18 No.2

목적: 석면에 노출된 근로자에서 석면관련 폐질환의 양상을 분석하고 진단에 있어 HRCT의 유용성을 연구하여 석면에 대한 의학적 감시프로그램의 개발에 기여하고자 하였다. 방법: 석면에 직접 노출되는 작업자 68명과 간접 노출되는 작업자 94명 등 총 162명에 대해 OSHA 석면 표준, 의학적 감시 프로그램에 따라 단순 흉부방사선검사, 폐활량검사 및 우리나라 실정에 맞게 일부 수정한 의학적 설문지를 통해 면접조사의 방식으로 환자에 관한 정보를 수집하였으며, 자료의 신뢰성을 확보하기 위하여 면접조사는 1달 이상의 기간을 두고 2차례에 걸쳐 실시된 후 확인, 수정하였다. 또한, 석면관련 폐질환을 확진하기 위하여 HRCT를 추가적으로 실시하였다. 결과: HRCT결과 석면관련 폐질환의 유병율은 162명의 근로자중 17명으로 10.5%였는데, 직접 노출군에서 10명 (14.7%), 간접 노출군에서 7명 (7.4%)이었다. HRCT와 비교할 때 단순 흉부방사선검사의 민감도와 특이도는 각각 70.6%와 98.6%이었고, 양성 예측도와 음성 예측도는 각각 85.7%와 96.6%으로, HRCT는 단순흉부방사선검사에 비해 민감도가 높아 초기 석면관련 폐질환을 진단하는데 효과적이었다. 석면관련 폐질환의 진단에 있어 HRCT와 폐활량검사 사이에 유의한 관련성이 관찰되었다. HRCT 결과 석면관련 폐질환의 유무와 의학적 설문조사 결과의 빈도분석을 실시한 결과, 유의한 변수는 작업기간, 흡연량, 호흡기질환의 과거력, 기침, 호흡곤란이었다. 결론: HRCT는 석면관련 폐질환의 진단에 있어 단순 흉부방사선검사와 비교하여 민감도가 높아 초기 진단에 유용하므로 단순 흉부방사선선검사 결과 석면관련 폐질환소견을 보일 때뿐만 아니라, 정상소견을 보이더라도 폐활량 검사결과와 근무력, 흡연력, 호흡기질환의 과거력, 기침, 호흡곤란 등의 호흡기 증상을 참고하여 HRCT검사를 고려해야 한다. 또한, 본 연구에서 관련성이 없었던 요인들에 대해서도 향후 연관성을 규명하는 광범위한 전향적 연구가 필요하다. Objectives: This study was carried out to improve the medical surveillance program of workers exposed to asbestos by examining the usefulness of High Resolution Computed Tomography (HRCT) in the diagnosis of asbestos-related lung disease. Methods: The study subjects comprised 162 workers in a ship-repairing yard, 68 of whom had been directly exposed to asbestos and 94 indirectly exposed. The 'Occupational Safety & Health Administration (OSHA) asbestos standard, medical surveilance program' and HRCT were conducted to analyze the aspects of the asbestos-related lung disease. The OSHA asbestos standard, medical surveilance program consists of simple chest x-ray, spirometry and medical questionnarie. Results: Seventeen (10.5%) of the 162 subjects, 10 (14.7%) directly exposed and 7 (7.4%) indirectly exposed, revealed asbestos-related lung disease on HRCT. The sensitivity and specificity of simple chest x-ray for asbestos-related lung disease were 70.6% and 98.6%, and the positive and negative predictive values were 85.7% and 96.6% respectively, as compared with HRCT, HRCT was an effective diagnostic tool especially to detect early asbestos-related lung disease. The study results indicated a relative significant association between the results of spirometry and HRCT. The variables significantly associated with asbestos-related lung disease on HRCT were work duration, smoking history (pack-years), past history of respiratory disease, cough and dyspnea. Conclusions: In the diagnosis of asbestos-related lung disease, HRCT should be considered not only for workers with positive findings on simple chest x-ray, but also workers with specific findings on spirometry, occupational history, smoking history, and past history of respiratory disease, or with respiratory symptoms such as cough and dyspnea.

국내 민간투자(SOC)사업의 효율화 방안에 대한 연구

이호경,김상철,윤준선,백준홍 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1

The purpose of this study is to examine the theoretical concept and practical example of Social Overhead Capital(SOC) project based on development of nation the world. Also, the critical access is to find the way we can develop domestic SOC project underway in private and nationwide. Furthermore, anther access is to propose clearly how to utilize BOT, BTO in financial, technical, CM method focusing on more systematic and effective result.

GIS와 유전자 Algorithm을 이용한 유지관리를 고려한 최적노선 선정

이철승,강인준,이준석,최현 釜山大學校 都市問題硏究所 2002 都市硏究報 Vol.13 No.-

Maintenance of roads is important thing and it should be considered in the plan of the road design process. Optimizing some road design characteristics in the plan may reduce road maintenance costs over the life of the road. Maintenance costs are relevent to sideslopes, cross section, and annual average daily traffic. Road can be chosen to minimize these costs, focusing on those conditions. Genetic algorithm, which is a good technic about optimization problem, is used to optimize those of characteristics. The purpose of this study is to choose a road by optimizing these characteristics. It was selected automatically by using GIS and GA and the result shows that sideslopes have influence on choosing optimal road relevent to maintenance.

항생제 다제내성균 Staphylococcus aureus SA2로부터 분리한 테트라사이클린 내성 플라스미드 pKH6의 염기서열

이대운,윤성준,김우구,신철교,임성환,이백락,문경호 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

한국에서 임산균주로부터 분리한 테트라사이클린(Tc) 내성 플라스미드 pKH6의 전체 염기서열을 결정하여 가장 대표적인 Tc 내성 플라스미드인 pT181과 비교하였다. pKH6의 전체 길이는 4439bp로 pT181과 동일하게 나타났으며 염기서열은 7개의 염기에서 차이를 보였다. 전부 다 염기쌍이 치환된 형태로 나타났으며 그 중 3개는 coding 부위에서 일어났다. coding 부위에서 일어난 경우에도 1개는 동일한 아미노산을 coding 하였기 때문에 pT181과 차이가 없었으며 RepC와 Pre 단백질에 있어 각각 1개의 아미노산에서 차이를 나타내었다. The complete nucleotide sequence of pKH6, a tetracycline-resistance (Tc^r) plasmid isolated from multi-drug resistant Staphlococcus aureus SA2, has been determined and compared with that of the staphylococcal Tc^r plasmid pT181. The nucleotide sequences of the two plasmids are in agreement except for 7 nucleotides. All differences are caused by base pair substitutions. Among 6 substitutions, 3 occurred in coding regions. However, only tow base substitutions in coding regions resulted in changes of amino acid sequences in two different ORFs of repC and Pre proteins.

TiO₂(Rutile)-H₂O 현탁용액의 유동성질에 미치는 농도 및 온도효과

이철우,한상준,김응렬 漢陽大學校 基礎科學硏究所 1990 基礎科學論文集 Vol.9 No.-

TiO₂- water 현탁계의 유동성질을 계의 농도와 온도의 변화에 따라 Couette 형회전 점도계를 이용하여 측정하였으며 Ree-Eyring flow equation으로부터 유도된 dilatant flow equation에 적용하여 고찰하였다. Rutile-water 현탁계는 30%wt 이상의 고농도에서는 thixotropy를 나타내고 25%wt 이하의 농도에서는 dilatancy를 나타내었으며 유동에 대한 활성화 에너지는 약 14Kcal/mol정도였다. 본연구에서는 유동성질간의 전이를 한 물질 내에서 구조의 형성과 파괴가 경쟁적으로 일어난다는 견지에서 고찰하였다. Rheological properties of Rutile-water suspension with various conditions of particle concentrations and temperatures. The rheologicl properties were measured using Couette type rotational viscometer. And we calculated flow parameters with applied to BKHR flow equation. Rutile-water suspension shows dilatancy at lower concentration relatively (at below 28%wt.) but at higher concentration(above 30%wt.) thixotropic flow was observed. And the activation energy for flow was about 14Kcal/mol. In this paper we studied this transition in the view point of competitive rate process between structure formation and deformation in the same material.

치료 불응성 Still's병에서 자가 조혈모세포 채집시 사용된 G-CSF에 의한 급성 악화 1예

이재웅,박성현,왕준광,오호석,최정혜,배상철,이영열,김인순,최일영,안명주 대한조혈모세포이식학회 2003 대한조혈모세포이식학회지 Vol.8 No.2

치료 불응성 자가면역질환에서 고용량 항암화학요법 및 자가 조혈모세포이식술이 시행되고 있으나 조혈모세포 가동화 및 이식술 시 사용되는 G-CSF에 의해 오히려 기존의 자가면역질환의 증세가 악화되었다는 보고가 있다. 저자들은 만성적으로 재발되는 Still's병 환자에서 조혈모세포 가동화를 위해 사용한 G-CSF에 의해 자가면역질환이 급성 악화된 1예를 경험하였기에 보고하는 바이다. High-dose immunosuppressive therapy with autologous stem cell transplantation is an increasingly used treatment for severe refractory autoimmune disorder including multiple sclerosis, rheumatoid arthritis, juvenile chronic arthritis, systemic lupus erythematosus. Although the optimal method of collecting stem cell is not determined, G-CSF-based mobilization is generally considered safe. However, worsening of disease status was reported in autoimmune disease undergoing mobilization with G-CSF. We report a 24-year-old male with refractory Still's disease who developed acute disease flare after administration of G-CSF for stem cell mobilization.

신생아에서 자세 및 경사도에 따른 경피적 산소와 이산화탄소의 분압 및 경피적 산소 포화도의 변화에 대한 연구

이상주,이동환,박준수,박상철,안재억 순천향대학교 1992 논문집 Vol.15 No.2

To determine the optimal positino for the newborn, tcp02, tcpc02 and Sp02 were monitored, in 21 healthy term infants, in 21 healthy preterm infants and in those infants with respiratory disease using the Radiometer(Cophenhagen) TCM2 TC oxygen monitor, ECM 20 TC carbon dixide monitor and PULSOX-7(Minolta) oxygen saturation monitor in both supine and prone position. Also, monitored with tilting in each position. When the healthy preterm infants were prone, tcP02 rose by a mean of 5.8mmHg(p=0.0185), an increase of 8.7%, and Sp02 was not significantly increased by a mean of 0.6%(p=0.1859). In those infants with respiratory disease, tcP02 was not significantly increased by a means of 6.9mm/Hg(p=0.1014), and Sa02 was not significantly changed. There was no change of tcPC02 in all infants. This improved oxygenation in the prone position appears to be the result of enhanced ventilation/perfusion ratios. Tilting the body in a 20head-down, or 20 head-down partially produce significant change in tcP02, or Sp02 in the newborns. These findings may have important implications in the management of preterm infants requiring neonatal intensive care and the infants with respiratry disease.

형상기억복합재료의 제조공정 및 신뢰성 평가

이진경,박영철,이규창,최일국,이준현 한국비파괴검사학회 2001 한국비파괴검사학회지 Vol.21 No.6

형상기억복합재료는 복합재료내에 압축잔류응력을 발생시킴으로써 재료의 인장강도를 증가시키는데 사용되어지고 있다. 본 연구에서는 형상기억복합재료를 제조하기 위하여 TiNi 강화재와 A16061 기지재를 사요하였으며 핫프레스 방법에 의해 TiNi/A16061 형상기억복합재료를 제조하였다. 그러나 핫 프레스 방법에 의해 제조된 형상기억복합재료는 하중을 받을시 강화재와 기지재 사이에 계면손상현상이 발생하였으며 이를 위하여 냉간압연을 실시하였다. 냉간압연을 받은 시험편은 냉간압연을 받지 않은 시험편에 비하여 인장강도가 현저히 증가하였다. 또한 본 연구에서는 고온에서 형상기억복합재료의 미시적 손상을 평가하기 위하여 음향방출기법을 이용하였다. Shape memory alloy has been used to improve the tensile strength of composite by the occurrence of compressive residual stress in matrix using its shape memory effect. In order to fabricate shape memory alloy composite, TiNi alloy and Al6061 were used as reinforcing material and matrix, respectively. In this study, TiNi/Al6061 shape memory alloy composite was made by using hot press method. However, the specimen fabricated by this method had the bonding problem at the boundary between TiNi fiber and Al matrix when the load was applied to it. A cold rolling was imposed to the specimen to improve the bonding effect. It was found that tensile strength of specimen subjected to cold rolling was more increased than that of specimen which did not underwent cold rolling. In addition, acoustic emission technique was used to quantify the microscopic damage behavior of cold rolled TiNi/Al6061 shape memory alloy composite at high temperature.