DJ-1 deficiency impairs synaptic vesicle endocytosis and reavailability at nerve terminals

Kyung, Jae Won,Kim, Jin-Mo,Lee, Wongyoung,Ha, Tae-Young,Cha, Seon-Heui,Chung, Kyung-Hwun,Choi, Dong-Joo,Jou, Ilo,Song, Woo Keun,Joe, Eun-Hye,Kim, Sung Hyun,Park, Sang Myun National Academy of Sciences 2018 Proceedings of the National Academy of Sciences Vol.115 No.7

<P>Mutations in DJ-1 (PARK7) are a known cause of early-onset autosomal recessive Parkinson's disease (PD). Accumulating evidence indicates that abnormalities of synaptic vesicle trafficking underlie the pathophysiological mechanism of PD. In the present study, we explored whether DJ-1 is involved in CNS synaptic function. DJ-1 deficiency impaired synaptic vesicle endocytosis and reavailability without inducing structural alterations in synapses. Familial mutants of DJ-1 (M26I, E64D, and L166P) were unable to rescue defective endocytosis of synaptic vesicles, whereas WT DJ-1 expression completely restored endocytic function in DJ-1 KO neurons. The defective synaptic endocytosis shown in DJ-1 KO neurons may be attributable to alterations in membrane cholesterol level. Thus, DJ-1 appears essential for synaptic vesicle endocytosis and reavailability, and impairment of this function by familial mutants of DJ-1 may be related to the pathogenesis of PD.</P>

공무원들의 인식에 나타난 경관자원 특성 분석 연구

박선명(Park Sun-Myung),박선경(Park Sun-Kyung),김주현(Kim Joo-Hyun),김종하(Kim Jong-Ha),이정호(Lee Jung-Ho),최무혁(Choi Moo-Hyuck) 대한건축학회 2010 대한건축학회논문집 Vol.26 No.11

In this study, to set up landscaping plans, characteristics of landscape were extracted based on opportunity and weaknesses factors of landscape after carrying out a questionnaire targeting officers in 23 cities and counties in Kyung-buk province and the findings are as following. First, in analyzing condition, component and value of landscape based on opportunity and weaknesses factors, for opportunity factors, it was found that the condition of landscape was quantitatively great, the component of landscape was industrially great and the value of landscape was great in possibility of development. Also, for weaknesses factors, the 'quality' was superior in its condition, the industry was superior in its 'component' and the shortage of artificial resources was superior in its 'value'. Secondly, in the relation between factors consisting opportunity and weaknesses resources, firstly, it was found that industry, city and nature are superior for opportunity factors, and culture, traffic and population are superior for weaknesses factors. Next, in the relation between component and condition, 'qualitatively superior industry' and 'quantitatively superior industry' had the highest frequencies in opportunity factors and 'qualitatively outstanding city' and 'quantitatively preponderant industry' had the highest frequencies in weaknesses factors. Thirdly, in analyzing the importance of opportunity and weaknesses factors, for opportunity factors, qualitatively superior resource was a priority in the importance of condition and for weaknesses factors, it was qualitatively little greater than the quantity. In the importance of component, the industry was most superior in both of strength and weak points and in the importance of value, the shortage of artificial resources was a priority. Fourthly, in characteristics of landscape, internal characteristics were found stronger than external characteristics in each city and county. This means that there are more resources to strategize in landscape plans of individual city and county unit than other in metropolitan unit. Also, possibility of development, possession of complex resources and geographical adjoin were common points of their strategies and plans.

식품 기준·규격의 합리적 관리 ·운영에 관한 연구(Ⅰ) : 두부,식용유지, 전분의 분말상 원료에 관하여 Powdered marerials of Tofu, Edible oil, Starch

김희연,홍진환,박혜경,한상배,박종석,이은주,이정성,송경희,최은희,최영준,소경아,성영제,이주엽 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

본 연구는 분말상 원료(전분, 대두분)의 저장유통시 품질변화를 건전성과 안전성 측면에서 검토하여 식품의 기준 ·규격 설정f.」 합지적으로 반영하고자 하였다. 전분과 패두분의 이물을 검사하고, 2종 대두분(중국산, 미국산)을 ?0일간 IS'c와 30t에 저장하면서 성상, 수분, 산가, 과산화물가, 지방산 조성,아플라톡신 BB의 생성여부를 분석하였다. 중국산 대두분 30'』 저장군에서 50일 이후 곰팡이가 발생한 것 이외에 모든 시료에서 양호한 성상을 나타내었고, 전분과 대두닥 모든 시료에서 이물은 검출되지 않았다. 산가는 중국산과 미국산 시료간에 편차를 보여 증국산 30"C, 50일 저장시료에서 약 10배끙도 증가하였고, 과산화물가는 저장 90일후 15'E 저장군은 약 2.3배, 30'c 저장군은 3배정도 증가하였다. 대두분의 주요 지방산은 linoleic acidf18. 2), oleic acid(18 : 1), palmitic acid(16 : 0)이었으며, 3개월 저장에 따른 지방산 함량의 변화는 미미하였다. 아플라톡신 B₁은 모든 시료에서 검출되지 않았다. The objective of this study was to investigate auality changes of type raw materials(starch, soy flour) during various storage conditions. Starchflour(source of China, U.S.A) were slfred under two temperature(15'C, 30'c) forSensory evaluation, (o.reign material test, moisture content, acid value, peroxide ualue,composition, aflatorin Bi were analyzed. China soy flour(stor·ed 30'f) was contaminated by fungj on 50 days. Except for soy flour contardnated by fungi, sensory characteristics were not changed and foreign mater;als were notdetected. Acid value of China soy flour(stored at 30'C, 90 days) was about 10 times higherlevel before storage and acid value was more influenced by source(China, USA). Peroxide valuewas in proportion with the storage period slightly. The major fatty acid of soy flour waslinoleic acid(18 . 2), an(3 the change of fatty acid composition was not observed. Aflatoxlrl Blwas not detected.

급성기 뇌경색 환자에서 화열 관련 증상과 증후의 변화에 관한 연구

곽승혁,박수경,우수경,이은찬,박주영,정우상,문상관,조기호,조승연,박성욱,고창남,Kwak, Seung-hyuk,Park, Su-kyung,Woo, Su-kyung,Lee, Eun-chan,Park, Joo-young,Jung, Woo-sang,Moon, Sang-kwan,Cho, Ki-ho,Cho, Seung-yeon,Park, Sung-wook,Ko, Ch 대한중풍순환신경학회 2011 대한중풍.순환신경학회지 Vol.12 No.1

Objective : Fire and heat related symptoms and signs are considered common in acute stage of diseases. The purpose of this study is to evaluate the occurrences and changes of fire and heat related symptoms and signs in acute cerebral infarction patients. Method & subjects : 40 acute cerebral infarction patients hospitalized in Oriental medicine hospital, Kyung-Hee University, who had examined and diagnosed 2 or 3 times based on oriental medical diagnosis were selected. We chose 23 as fire and heat related symptoms and signs from 94 diagnostic articles, and we added all those scores together of each patient. We analysed the scores of fire and heat related symptoms and signs as the time passed, and depending on oriental medical diagnosis. Result : In acute cerebral infarction patients of this study, 4 of fire and heat related symptoms and signs were took 1st, 2nd, 4th and 8th places in most changeable 10 articles of total 94 articles. The mean score of fire and heat related symptoms and signs of all patients were decreased significantly over the 3 times of measurements. The 8 patients diagnosed as fire and heat diagnosis at visit1 were samely diagnosed as fire and heat diagnosis at visit2, and at visit3 5 patients of them except for 3 patients excluded between visit2 and visit3, were still diagnosed as fire and heat diagnosis. At all of 3 measuring times, the scores of fire and heat related symptoms and signs of fire and heat diagnosis group were higher than non-fire and heat diagnosis group. Conclusion : This study indicated that fire and heat related symptoms and signs were very changeable phenomenon in acute cerebral infarction patients. And they decreased as time goes on.

가야산 국립공원일대의 식생 및 식물상 연구 : 단지봉 지역을 중심으로 Especially on the Danji-bong Area

박광우,권영한,최경,오승환,김동갑,김주환 한국환경생물학회 2005 환경생물 : 환경생물학회지 Vol.23 No.1

본 조사지역인 가야산국립공원은 지리학적으로는 북위 35?5′00″~35?9′30″, 동경 128?2′30″~128?09′30″에 위치하고, 행적구역상으로는 경상남도의 합천군과 거창군, 경상북도의 성주군, 고령군에 걸쳐 있으며, 총면적은 57.81 km꼬?이른다. 가야산은 우리나라 기후지역상 온대남부에 위치하고, 식물구계학적으로는 남부아구에, 식생구계학상으로는 냉온대에 속하는 것으로 알려져 있다. 또한, 전체면적의 약 82.5%가 해발 600m 이상이며, 1,000m이상이 약 10.3%에 해당된다. 가야산의 해인사-극락골-토신골 지역은 잘 보존된 소나무림이 우점하고 있고, 부분적으로 노각나무가 산생하는 것이 관찰되었으며, 해인사-진대밭골-두리봉 지역은 계곡에는 소나무와 졸참나무가 고지대에는 신갈나무 등이 부분적으로 군락을 형성하고 있었다. 또한 남산제일봉과 북사면은 각각 등산로와 임도가 형성되어 있어 소나무와 졸참나무, 신갈나무 등이 혼재된 양상을 나타내었고, 백운동계곡 주변은 다른 사면과 달리 식생의 발달정도가 소나무와 관목위주로 매우 단순했다. 특기할만한 점은 상왕봉 주변에서 흰참꽃, 설앵초, 네귀쓴풀, 백리향, 솔나리, 구름송이풀 등의 희귀 또는 고산성 식물들이 분포하는 것이 확인되었다. 가야산국립공원지역에 분포하는 양치식물 이상의 관속식물을 대상으로 조사한 결과 91과 268속 373종 1아종 75변종 12품종 4잡종으로 총 465분류군이 조사 확인되었으며, 이는 우리나라 관속식물 4,071 분류군의 11.4%에 해당되었다. 한국특산식물은 11과 15속 15분류군으로 조사지역내 분포하는 465분류군중 약 3.2%이며. 식물구계학적 특정식물은 17과 21속 25분류군으로 이는 가야산 소산식물 465분류군의 5.4%이었고, 조사지역에 분포하는 귀화식물은 5과 7속 7분류군으로 우리 나라전체 귀화식물종 177종류의 4%이다. 또한 가야산 단지봉(1,028.5m)에서 새로 발견된 산간습지 (forested wetland)와 자란초 자생 군락지에 대해 식생 및 층위별 종조성을 토대로 현존 식생 구조를 분석하였다. This study aims to investigate the flora of Mt. Gaya National Park, especially focused on the forested wetland vegetation of Danji-bong area. The flora of Mt. Gaya was investigated from April in 2003 to October in 2004. Vascular plants of this area consist of total 465 taxa, 4 hybrids, 12 forma, 75 varieties, 1 subspecies, 373 species, 268 genera, 91 families. It corresponds to 11.4% of totally 4,071 taxa appeared in Korea. Korean endemic plants were composed 11 families, 15 genera, 14 species, 1 variety and totaling 15 taxa. And also actual vegetation structures were analyzed the newly founded forested wetland and natural population Ajuga spectabilis on the Danji- bong area using the vegetation and the species composition by tree layer.

설비보전관리와 안전정보를 연계한 Web 상에서 지식베이스 진단시스템 구현

박주식,강경식 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

To keep enterprise's competitiveness on condition of the automatic manufacturing system such as FA, FMS and CIM, all the maintenance problems should be considered seriously in not only production and maintenance but also related industrial safety. As we analyze in the surveys for the maintenance management of domestic enterprises and the causes of industrial accident, there will be necessity of drawing up countermeasures for prevention of industrial accidents and for ensuring expertise maintenance technologies. Based on these analyses, this study studied the safety information system, maintenance management information system, and the machinery condition diagnosis technique by using of the knowledge-based system under the internet environment. This web based knowledge-based diagnosis system can easily provide not only the knowledge of expert about deterioration phenomenon of industrial robot, but also the knowledge of relating safety and facility on everywhere, everytime, Therefore, when we use this system, it is expected to improve the efficiency of business processes in the production and safety.

음악요법이 위내시경 대상자의 검사전 불안에 미치는 효과

박경숙,나명주 성인간호학회 2003 성인간호학회지 Vol.15 No.2

This study examined the effect of music therapy on the pre-exam of gastroscopy clients. Method: The research period is from June 17 2002 to August 30 2002, and the subjects were 60 individuals, 30 for the experimental and 30 for the control group among adult clients. The data were analyzed by using the SPSS program. Result: The results of this study were as follows: The score of the experimental group was lower than the score of the control group for the situational anxiety (t=-12.56, p=0.000) and systolic blood pressure (t=-3.97, p=0.000) before the examination was supported. The vital sign was partially supported. There was no significant difference(t=-1.05, p=0.297) in rhe diastolic blood pressure (t=-1.05, p=0.297) and pulse rate(t=-1.30, p=0.199) before the examination of the experimental group and lower than the score of the control group. Conclusion: Music therapy could be useful in the nursing practice, and be utilized as a way of nursing intervention to reduce anxiety of the patients in specific clinical situations. Finally, it is recommended that further studies be conducted on this therapy by utilizing different musical selections.

한국 노인의 특성에 따른 맛 감지 능력의 차이에 관한 연구

박경애,이희주,박명숙,이정희,전상은,김종성,최스미 대한노인병학회 2002 대한노인병학회지 Vol.6 No.4

CARBAMATE의 새 합성방법에 대하여(제1보)

주완철,박경래 成均館大學校 1979 論文集 Vol.26 No.-

Previous investigators have prepared carbamates by the Hoffmann-like rearrangement of amides using lead tetraacetate as an oxidation agent. Almost of these investigations are concerned mainly with the procedures of the carbamate preparations. For the formation of carbamates by the Hoffmann-like rearrangement of amides it seems very important to know the influences of the character of amides and also the acidity of alcohols. In this work we have studied at first the influences of amides with electron withdrowing and donating character upon the formation of carbamates. From our results we have found that there is no remarkable influence of amides on account of the yield or the reactivity. We have also found that this reaction does not initiate without base. Therefore we have concluded that the formation of isocyanate intermediate in the process of carbamate preparation occurs by a spontaneous intramolecular synchronous rearrangement and not by the free nitrene formation, and that the catalytic effect of a base, which acts to displace a nitrogen bonded proton of the amide and initiates the reaction, must be accounted as an important factor.

호흡 부전을 동반한 급성 자율-감각신경병증 1예

박경필,안규환,김은주,김대성,정대수,박규현,김매자 대한신경과학회 1999 대한신경과학회지 Vol.17 No.5