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박혜경,박낭운,이병헌,최제용,김인산,조준승 慶北大學校 醫科大學 1995 慶北醫大誌 Vol.36 No.2
목적 :이 연구의 목적은 keloid가 점점 그 주위조직으로 침범하는 성질이 있는 것에 착안하여 keloid 중심부위와 그 인접부위 그리고 정상부위 조직에서의 collagen 합성능을 측정하여 상호비교 해보는데 있다. 재료 및 방법 : 7예의 keloid 섬유아세포와 3예의 keloid 인접부위 섬유아세포 그리고 5예의 정상부위 섬유아세포를 배양하여 Peterkofsky씨등의 방법^13)에 따라 collagen 합성능을 측정하였다. 해체와 배양조건에 따른 차이를 배제하기 위하여 한 환자로부터 동시에 각 부위의 조직을 동시에 얻어 동시에 섬유아세포를 배양하였다. 결과 : Collagen 합성능의 평균값은 정상조직보다 keloid 부위에서 훨씬 높았으며 또한 keloid 부위보다 그 인접부위에서 더욱 높았다. 결론 : 섬유아세포에서의 collagen 합성능은 정상부위, keloid 부위 및 그 인접부위 순으로 더욱 높았다. 이것은 keloid 환부주위에 국소적으로 작용하는 인자들이 존재하여 이들에 의해 keloid 주위조직이 자극을 받아 collagen 합성능이 증가하게 됨을 시사한다. Keloids are predominantly fibrous skin tumors that take the form of firm, variably pruritic or tender growths near the site of an injury. Histopathologically they are ricd in various connetive tissue components, such as collagen. In this study, the collagen synthesis by fibroblasts derived from keloid, keloid-adjacent area and normal skin was analyzed. Six keloid fibroblast cell lines out of 7 demonstrated increased collagen synthesis and one showed collagen synthetic rate that was within the control limits. One keloid fibroblast cell and one normal fibroblast cell were cultured from a patient, and when their collagen synthetic rates were compared with each other it resulted in that keloid cells showed a higer synthetic rate than normal cells. Three keloid-adjacent fibrolast cell lines showed markedly incerased collagen synthesis than their corresponding keloid cell lines which were derived from the same patients. Taken together, these results suggest that in keloid lesion, there might be local factors which stimulate surrounding fibroblasts to synthesize excessive collagen.
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Polyacrylamide Gel Electrophoresis를 이용한 설사 환아 대변 검체에서 로타바이러스의 Electropherotype 조사
박혜경,서주영 이화여자대학교 생명과학연구소 1994 생명과학연구논문집 Vol.5 No.-
Rotavirus is known to be important causative agent of acute gastroenteritis in infants and young children all over the world. They also reported that rotavirus infection was prevalent in Korea. For the purpose of serosurvey and subgrouping of rotaviruses from children hospitalized for diarrhea in Korea, the stool samples were analysed for RNA electrophoretic pattern. The stool samples were also immunoassayed for the presence of rotavirus antigen and the sera were checked for the presence of antibody reacting with rotavirus antigen. Stools and sera from 28 infants or children(mean age 1.72 year) hospitalized for diarrhea in Ewha Womans University hospital from Dec. 1992 to Dec. 1993 were analysed. Enzyme-linked immunosorbent assay showed taht 89.3%(25/28) of the children with diarrhea had serum antibody reacting with rotavirus. Dot blot immunoassay showed that 89.3%(25/28) of the stools from children with diarrhea had rotavirus antigen. Two children were negative both for stool rotavirus antigen and for serum antibody to rotavirus. However, two children were postive only for either stool antigen or serum antibody. The silver staining patern of SDS-PAGE of RNAs extracted from stools revealed four electropherotypes; typical shor(subgroupⅠ), and typical long(subgroupⅡ) pattern with eleven bands, and long narrow and long wide patterns in which the 5th and the 6th bands were narrow and wide, respectively. Among the 28 samples, 20(71.4%) samples could be analysed for electropherotypes of rotavirus. The electropherotype distributhion was as follows; 35%(7/20) were long pattern, 20%(4/20) were long wide pattern, 15%(3/20) were long narrow pattern, and 30%(6/20) were short pattern. The stool samples from the two children negative both for stool antigen and for serum antibody were also negative for rotavirus RNA electropherotype. The detection of rotavirus RNA was parallel with the detection of rotavirus antigen by dot blot immunoassay when the serum antibody and the stool antigen was contradictory. These results suggested that long patterns(70%) of rotaviruses were prevalent in Korea. And the detection rate of rotavirus by RNA electropherotyping of stool samples was slightly less than that by dot blot immunoassay.
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박혜경,구현아 동덕여자대학교 2001 생활과학연구 Vol.6 No.-
This study was conducted to investigate how young children recognize the body organs. One hundred and twenty six children of ages 3, 4 and 5 were interviewed at an affiliated kindergarten of a university located in Seoul. Pictures of body organs such as eye, nose, mouse, ear, hand, and foot were shown to the children and they were asked to describe what the pictures reminded them of. Their answers were classified as functional ones and descriptive ones; The former involves factual explanations of the body organs while the latter involves indirect and metaphorical expressions. The children usually used functional expressions when they mentioned about hand, foot, and mouse. It was also observed that the ways the children recognize the body organs differed according to their age or sex.
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