김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

뇌졸중 후 중추성 통증 환자에 대한 동서협진이 진통과 재활에 미치는 영향

이현종,김수영,이상훈,서동민,이두익,김건식,이재동,이윤호,양형인,박재경,최도영 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2003 東西醫學硏究所 論文集 Vol.2003 No.-

Purpose : In order to study the effectiveness of East-West pain treatment on central poststroke pain(CPSP), we evaluated its effect on alleviation of pain and rehabilitation of CPSP Patients who were treated with electroacupuncture and west pain treatment for four weeks. Methods : Twenty four patients diagnosed by their pain characteristics of central pain from stroke were treated with sympathetic nerve block, gabapentin, amitriptyline, and electroacupuncture for four weeks. Pain intensity through the visual analogue scale(VAS), and improvements of mobility and rehabilitation through the modified Barthel index(MBI) and Rankin scale(RS), respectively, before and after pain treatment were also assessed. Results : VAS pain scores were significantly improved from 7.7±1.7 to 4.4±2.0 with pain treatment(p<0.05). In accordance with improvement of pain scores, RS and MBI scores were also improved from 2.88±0.95 to 2.13± 1.01 and from 83.0± 16.9 to 94.7±9.5(p<0.05), respectively, with pain treatment(p<0.05). Conclusions : It was suggested that the active pain treatment was contributed to the rehabilitation of CPSP patients, resulting in improvement of quality of life of CPSP patients. Futhermore, East pain treatment in combination with West pain treatment may be useful modality to alleviate CPSP.

소 수정란의 공배양 및 동결이 체외발생에 미치는 영향에 관한 연구

이종진,이명헌,남윤이,이만휘,이봉구,김상근 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

The studies on the carried out to investigate the effects of co-culture with cumulus cells and oviduct epithelial cells on the in vitro fertilization and cleavage rate of bovine follicular oocytes, and to determine the optimum thawing temperature and equilibration time of frozen bovine embryos and on survival rate and in vitro developmental rate of bovine embryos. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes were cultured in TCM-199 medium containing 10 IU/㎖의 PMSG(Sigma, USA), 10 IU/㎖의 hCG, 1 ㎍/㎖의 β-estradiol(Sigma, USA) and 10% FCS(Sigma, USA) for 24-48 hrs in incubator with 5% CO_2 in air at 38.5℃ and then matured oocytes were again cultured for 12-18 hrs with motile capacitated sperm by preincubation of heparin. The bovine embryos following dehydration by cryoprotective agents and a various concentrations of sucrose were directly plunged into liquid nitrogen and thawed in 30℃ water. The results are summarized as followes : 1. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with cumulus cells in TCM-199 media were 75.0%-76.8% and 17.3%-27.6%, respectively. And in-vitro fertilization rates of cumulus-enclosed oocytes(55.4%) were significantly(p<0.05) higher than cumulus-denuded oocytes(23.1%). 2. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with 1 ×10^4 cells/㎖, 1 ×10^6 cells/㎖, 1 ×10^8 cells/㎖ and 1 ×10^15 cells/㎖ oviduct epithelial cells in TCM-199 media were 74.5%-77.8% and 15.7%-21.2%, respectively. 3. The in-vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured in TCM-199 media containing PMSG, hCG, PMSG + hCG, PMSG + β-estradiol, hCG + β-estradiol 0 to 40 hrs after insemination were 74.0%-77.4% and 18.9% - 23.1, respectively. 4. The survival rates of bovine embryos thawed after rapid freezing in the media containing various kinds of cryoprotective agents added 0.25M and 0.50M sucrose were 14.7% - 35.1% and 17.6% - 31.6%, respectively. The survival rates of bovine embryos thawed after rapid freezing in the freezing media containing a various concentration of sucrose added 1.5M and 2.0M glycerol, 1.5M and 2.0M DMSO, and 1.5M and 2.0M propanediol were 23.5% -31.4% and 20.6% - 34.1%, respectively. 5. The temperature thawed at 30℃ after rapid freezing of bovine embryos resulted in a significantly higher embryos survival rate than did at 20℃ and 35℃. 6. The equilibration time on the survival rates of bovine embryos was attained after short period of time(2.5-5 min.) in the freezing medium higher than ling period of time(10-20min.).

다중 속성의 가중치를 이용한 인터넷 채용 협상 시스템의 설계 및 구현

이근수,이근왕,이종희 안성산업대학교 2002 論文集 Vol.33 No.-

These day, Internet Recruiting needs the negotiation of recruiting items. This paper proposes multilateral negotiation that substitutes applicants and employers. Previous Negotiation Systems use the preference value of the multi-attribute and the sequential negotiation. But proposed Internet Recruiting Negotiation System(IRNS) uses the parallel negotiation of multi-attribute. The parallel negotiation supplies the multi-attribute negotiation including values of single-attribute and result of parallel negotiation. This paper shows that excellent result for effective negotiation using the weight strategy of multi-attribute for a matter of concern of users.

Southern Blot Hybridization법을 이용한 악성 림프종의 면역유전형에 관한 연구

전호종,기근홍,김윤신,이미숙,이미자,장원재,장숙진,박영진,정춘해,정종훈,양성훈,이광민 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.2

Immunogenotyping using gene rearrangement analysis has emerged as a precise laboratory aid in the diagnosis and classification of malignant Iymphoid neoplasms. The lineage and clonality of the malignant Iymphoid neoplasms can be identified by the demonstration of rearrangements of antigen receptor genes of the immunoglobulin and T-cell receptor genes. The analysis of the gene rearrangements on the malignant Iymphoid neoplasms are also useful as a sensitive unique clonal markers to detect early recurrence in patients with malignant Iymphoid neoplasms after treatment. To analyze the sensitivity and specificity of gene rearrangements in the diagnosis of malignant Iymphoid neoplasms. 24 cases of malignat Iymphoma were examined by Southern blot hybridization using CTβ-T cell receptor β chain gene-DNA probe and JH-immunoglobulin heavy chain gene-DNA probe. The results of the immunogenotypings using Southern blot hybridization disclosed high correlation between the immunophenotyping using immunohistochemical stain with monoclonal antibodies (B-cell Iymphoma 84.2%. T-cell Iymphoma 75% ). The analysis of the gene rearrangement of the angioimmunoblastic lymphadenopathy(AILD) and unclassifiable Iymphoma using immunohistochemical stain could resolve the monoclonality and lineage . Rearranged bands to the CTβDNA probe were observed in one case out of 2 cases of AILD. One case of unclassifiable Iymphoma showed rearranged bands to the CTβ DNA probe. There were no rearrangements in reactive follicular Iymphoid hyperplasia and paracortical Iymphoid hyperplasia. In conclusion, DNA gene rearrangement study should be applied to differentiate the clonality and cell lineage in the malignant Iymphoma with indistinctive immunophenotype.

保險契約法上의 obliegenheit (Ⅱ)

李鍾根 경북대학교 경제경영연구소 1991 經商論集 Vol.19 No.2

赤米의 Anthocyan 色素에 關한 硏究

이란숙,박종대,박근형 全南大學校 農業科學技術硏究所 1993 農業科學技術硏究 Vol.28 No.-

상해향혈나 赤米 색소의 식품가공용으로서의 이용가능성에 대한 기초조사로서 赤米 색소의 이화학적 성질과 총 anthocyanin 함량 측정 결과는 다음과 같다. 1. 赤米 색소 추출물은 0.1N NaOHdp 의해 청색으로 변했고, 다시 0.1N HCl을 가해보니 본래의 색으로 복원되었으며, ether와 chloroform에는 전혀 녹지 않아 赤米색소 추출물은 anthocyanrP임을 추정할 수 있었다. 2. 赤米의 anthocyan 색소는 aluminum chloride, ammonium molybdate, 그리고 lead acetate 시약 처리에 의해 청색으로 변색되어 赤米 색소는 cyanidin, delphinidin, petunidin계 中 어느 한계에 속하는 색소임을 추정할 수 있었다. 3. 赤米 색소의 흡수 spectrum은 전형적인 anthocyanin의 흡수 spectrum을 보였으며, 흡수극대파장은 530nm, ????/???? (%) 比값은 19.4를 나타냈다. 그리고 5% ???? ethanol 용액 첨가에 의해 장파장 쪽으로 이동함을 보여 이상의 분광학적인 성질을 종합해 본 결과 赤米 색소 anthocyanin은 cyanidin 계의 3-monoglucoside 배당체 임을 추정할 수 있었다. 4. 赤米 색소의 TLC와 PC 분석 결과 각각의 용매계에서 단일 spot를 보여 단일종의 anthocyanin으로 구성되었음이 시사되었으나 HPLC 분석 결과 90% 이상을 차지하는 주 peak외에 작은 peak가 인정되었다. 5. 주 색소가 cyanidin-d-monoglucoside로 알려진 자두의 anthocyan 색소를 동일한 조건의 HPLC를 행한 결과 赤米의 주 anthocyan 색소의 data와 일치하여 赤米의 anthocyan 색소는 cyanidin-3-mono-glucoside로 확인되었다. 6. 赤米 색소의 총 anthocyan 함량은 14.3% 수분량을 갖는 赤米 100 g당 cyanidin-3-monoglucoside 형태로 40.3 mg 있었다. 이상의 결과를 종합해 볼 때 赤米 색소는 cyanidin-d-monoglucoside가 주 색소를 이루었으며, 총 anthocyan 함량은 赤米 100g당 cyanidin-3-monoglucoside 형태로 40.3mg 있었다. This study investigated red rice pigments with red color as a possibility of use them in the food processing. In order to separate and identify the pigments, the red rice of sanghaehyanghyulna was extracted with hydrochloric acid in methanol. The pigments were identified as the anthocyan by qualitative test. Base on the separated pattern thin layer chromatography, paper chromatography and spectrophotometric characteristics, the main anthocyanin of red rice pigments was identified as cyanidin-3-monoglucoside. This conclusion was supported by the comparison with high performance liquid chromatography chromatogram of the plum pigments in which the major anthocyanin was reported cyanidin-3-monoglucoside. The total anthocyanin content in red rice pigments was calculated 40.3 mg/100g weight.

광주시내 업종별 음식점의 주방기구와 관련된 주요 세균 및 이들의 계절별 변화

이홍열,유맹자,정해진,김근영,정희종 全南大學校 農業科學技術硏究所 1998 農業科學技術硏究 Vol.33 No.-

업종별 음식점에서 사용중인 주방기구 가운데 개숫물중에 존재하는 세균들을 분리·동정한 결과, 한색음식점에서 37균주, 일식음식점에서 23균주, 및 중식음식점에서 33균주가 분리되었고 이들 중 한식음식점에서 E. coli 등 16균주, 일식음식점에서 Listeria sp. 등 20균주, 중식음식점에서 Micrococcus sp. 등 15균주를 각각 동정하였다. 이들 세균들은 업종별 음식업소에서 주로 사용되는 색품재료에 존재하는 세균들과 밀접한 관계가 있는 것으로 밝혀졌고 E.coli와 같은 일부 세균들은 주방의 불결한 환경이나 종사자로부터 오염된 것으로 추정되었다. 업종별 음식점에서 사용중인 개숫물, 행주, 칼, 및 도마에 존재하는 세균의 분포는 일반세균수 및 대장균수가 모두 2월에서 8월까지 점점 증가하다가 11월에는 다시 감소하는 경향을 보였다. 특히 이같은 경향은 일반세균은 개숫물에서 대장균군은 칼에서 가장 뚜렷하게 나타났다. 따라서 식중독 발생 가능성이 가장 높은 여름철에는 칼, 도마 등 주방기구들은 정기적으로 열탕소독하는 철저한 위생처리가 필수적인 것으로 생각되었다. Thirty-seven, twenty-three, and thirty-three bacterial strains were isolated from dish-washing water which was selected as a kitchen supply commonly loaded the highest level of bacteria among various supplies using at Korean style, Japanese style, and Chinese style restaurants. Among these isolates, sixteen strains including Esherichia coli, twenty strains including Listeria sp., and fifteen strains including Micrococcus sp. were identified from Korean style, Japanese style, and Chinese style restaurants, respectively. This result suggested that most isolated bacteria were closely related to bacterial flora of raw food materials used in each style of restaurants and others could be contaminated from the surrounding environment and the employees. Total and coliform bacterial counts distributed in dish washing water, wiping cloths, knives, and cutting boards used at different types of restaurants were gradually increased from February to August and then decreased until November. This changing tendencies of total bacterial counts and coliform group counts were obvious in dish-washing water and in knives, respectively. Therefore, kitchen supplies such as wiping cloths, cutting boards, and knives should be treated carefully with hot water of above 45℃ at every certain period of time to kill some of these potential hazardous bacteria and to prevent outbreaking foodborne-illness by them, especially during Summer.

표면 SH파 모드의 분산특성 해석과 그 응용

이상용,박익근,윤종학,노승남,안형근 한국공작기계학회 2001 한국공작기계학회 춘계학술대회논문집 Vol.2001 No.-

A new flaw detection technique using by SH angle beam method has been discussed. The SH-wave is horizontally polarized shear wave and the surface SH wave has a characteristic of traveling along near surface layer. The surface SH wave technique is valuable for the detection of fatigue cracks at fillet weld heels which cannot be detected by other ultrasonic technique such as angle beam technique and The dispersion curves of it has simple characterization. In this work, using these beneficial chraterization, quality evaluation of spot weld with ultrasonic sound intensity of SH-wave passing through nugget area of spot weld are verified experimentally.

흰쥐 해마에서 일시적 대뇌 허혈에 의한 glutamate 수용체 아형 유전자의 발현변동

이승한,김종근,배춘상,정윤영,조기현 全南大醫大 2001 전남의대학술지 Vol.37 No.3