유산균(L.lactis CBT-8)및 발효 추출물의 Helicobacter pylori 억제 능력

진춘조,박형석,이혜운,김성렬,강동구,이준상,이재동,왕준호 건국대학교 의과학연구소 2002 건국의과학학술지 Vol.12 No.-

Helicobacter pylori infection was the cause of chronic gastritis, duodenal ulcer and gastric MALT lymphoma. The eradication rates of H. pylori using antibiotics are around 80%. Lactobacilli have been demonstrated to have in vivo and in vitro inhibitory effects on H. pylori infection. We aimed to evaluate the inhibitory effect of L. lactis CBT-8 and its extract on H. pylori in human stomach. Thirty-five H. pylori-infected volunteers(30-49 yr)were randomized into two groups which were treated with L. lactis CBT-8 and its extract(Group A n=17) or milk containing L. lactis CBT-8(Group B, n=18). They underwent 13C-urea breath tests before and after treatment. The DOB30 of group A(n=14) was decreased 41.0% after treatment(26.0±4.9 before, 15.3±2.1 after treatment)(p<0.05). the DOB30 of group B(n=13) was decreased 22.3% after treatment(22.0±2.2 before, 17.1±1.7 after treatment)(p<0.05). In conclusion, L. lactis CBT-8 was effective in suppressing H. pylori infection in human stomach.

道와 存在 : 노자와 하이데거의 근본사상 비교

全東震 水原大學校 1997 論文集 Vol.15 No.-

Dieser Aufsatz erortert die gedankliche Nahe und Ferne zwischen Heidegger und Lao-tzu. Das namenlose 'Tao' des Lao-tzu entspricht nicht Heideggers 'Sein' bzw. 'Ereignis'. Von Heidegger her gesehen ist es ein metaphysisches Wort. Aber Lao-tzus Konzeption von der Weghaftigkeit als Grundcharakter allen Ph anomens und dem 'Nicht-Tun' als der Weise der ursprunglichen Antwort des Menschen zu dem in sich bewegten Phanomen zeigt eine Verwandtschaft mit Heideggers Konzeption des weghaften Seins und der 'Gelassenheit'.

약물 유해반응 보고지침 -임상시험의 안전성에 관한 정보 관리지침 -

지동현,김훈교,배진우,손동렬,김인범,전용관,박혜연 大韓臨床藥理學會 1998 臨床藥理學會誌 Vol.6 No.2

일측 자골신경의 절단 및 재연결 후 흰쥐 척수에서 신경세포 접착분자(NCAM)의 면역조직반응의 변화에 관한 형태학적 연구

황세진,전복관,안동춘,조원길,김승현 대한신경과학회 1999 대한신경과학회지 Vol.17 No.1

하이데거의 존재론적 차이에 대해

全東震 水原大學校 1993 論文集 Vol.11 No.-

Die "ontologische Differenz", d.h. die Differenz von Sein und Seiendem nennt die Sache des Denkens, auf die Heideggers Denken standig unterwegs war. Der spate Heidegger faβt ontologische Differenz als den "Unter-Schied" von Lichtung und Anwesenheit auf. Dieser Unter-Schied ist ein Grundverhaltnis in dem je konkert ereignenden Seinsgeschehen, das der spate Heidegger als "Ereignis" zu denken versucht. Diese Grundstruktur des Ereignisses kommt aber schon in der "fundamentalontologischen" Frage nach dem "Sinn von Sein uberhaupt" als das Verhaltnis von Sein (des Innerweltlichen) und Zeit (der Welt) zur Sprache. So wird in diesem Aufsatz zunachst die Intention der Seinsfrage geklart und dann das Verhaltnis von Lichtung und Anwesen. Auβerdem wird gezeigt, daβ die Metaphysik ihre "onto-theologische". Wesensverfassung gerade der Verstellung des Unter-Schiedes von Lichtung und Anwesenheit verdankt.

숯 처리에 의한 녹차 추출액 중 질산태질소 제거 효과

김진우,고영옥,신동국,이 협,전현식,이종옥 진주산업대학교 산업과학기술연구소 2005 산업과학기술연구소보 Vol.- No.12

This experiment was carried out to measure the elimination effect of nitrate nitrogen from green tea extracts through the column system filled with charcoal activated and bamboo charcoal powder compare to that of an nitrate nitrogen standard solution prepared with KNO3 chemicals. The change patterns of pH, and the amount of removed nitrate nitrogen from green tea and nitrate nitrogen standard solution were analyzed according to time course and the amount of charcoal treatments. The results were summarized as follow: 1. pH The pH of Green tea extractions treated with charcoal was high up to 8.17(after 30min treated with 0.4% charcoal) and 8.36(after 60min with 0.4% charcoal) which pH of non-treated green tea (control) was 5.8 The results indicated that the pH values were variable to the treated amount of charcoal and the teated time course. 2. Nitrate nitrogen From the nitrate nitrogen standard solution, the least rate 69.1% and the highest rate 74.5% of nitrate nitrogen were observed to be absorbed through the column treatment (60 min), which with green tea extract solution 21.3% (the least) and 43.8% (the highest) were detected to be absorbed through column treatment (after 60min with 0.4% charcoal). In the case of bamboo treatments with nitrate nitrogen solution samples were showed the least rate 62.8% (30min with 0.1% bamboo) and the highest rate 68.9% (60min with 0.4% bamboo), and with green tea extract solution were observed the least 13.6 (30min with 0.1% bamboo) the highest rate 29.4% (60min with 0.4% bamboo). In general, the elimination activity of nitrate nitrogen was higher by the charcoal activated powder treatment than bamboo charcoal.

호중구 감소성 발열환자에게 경험적으로 투여한 Teicoplanin의 효과

이동건,임동석,최수미,박선희,유진홍,최정현,민우성,신완식,김춘추 대한감염학회 2004 감염과 화학요법 Vol.36 No.2

목적 : 호중구감소성 발열 환자에게 경험적으로 teicoplanin을 투여할 때의 효과를 알아보고자 전향적 연구를 시행하였다. 방법 : 2003년 7월부터 12월까지 가톨릭조혈모세포이식센터에 입원하여 항암치료 혹은 조혈모세포이식을 시행하고 호중구감소성 발열이 있는 환자 중 초기 항균요법에 반응이 없어 경험적 teicoplanin 투여가 필요한 49명을 대상으로 A, B 제조회사에서 제공한 teicoplanin을 무작위로 어느 한 쪽 치료군에 배정하여 투여하였다. 용량은 첫날 400㎎ 부하용량을 정맥내 bolus로 투여하고 매 24시간마다 200㎎ 유지용량을 투여하였다. 결과 : A군 27명, B군 22명이 연구에 참여하였고 대부분의 환자가 신독성이 있는 약제를 병용하고 있었다. A군 8명, B군 7명에서 그람양성균이 동정되었고, teicoplanin에 대한 내성률은 A군 22.2%, B군 28.6%로 유의한 차이는 없었다(P=1.00; 0.61<95%CI<1.95). 미생물학적 확인 감염이 있었던 환자 중 평균 53.3%에서 완치 혹은 개선의 반응이 있었고 양 군간 유의한 차이는 없었다(A군 4명 [50.0%], B군 4명 [57.1%], P=1.00; 0.29<95%CI<2.60). 미생물학적 제거율은 평균 62.5% (A군 55.6%, B군 71.4%)이었고 그 외 미생물학적 효과는 A군에서 제거 후 재발 2명(22.2%), 내성 2명(22.2%)이었고 B군에서 각각 0명(0.0%), 2명(28.6%)이었으며 양 군간 유의한 차이는 없었다(P=0.28). 발열기간(P=0.89), teicoplanin 사용기간(P=0.47) 및 전체적인 사망률(P=1.00; 0.78<95%CI<1.24)도 양 군간 유의한 차이는 없었다. 이상반응 중 신독성은 16.3% (A군 18.5%, B군 13.6%)에서 나타났고 양 군에 차이는 없었으며(P=0.72; 0.39<95%CI<3.51), 신기능 이상과 관련있는 약제를 적어도 2개 이상 병용하고 있었다. 피부발진은 A군에서 1명, B군에서 3명 발생하였다(P=0.31; 0.93<95%CI<1.34). 결론 : 호중구감소성 발열환자에게 teicoplanin을 투여하였을 때 임상적 반응률은 평균 53.3%(A군 50.5%, B군 57.1%), 미생물학적 제거율은 평균 62.5%(A군 55.6%, B군 71.4%)이었고 두 제조회사간 차이가 없었고 이상반응도 양 군간 차이가 없었다. 앞으로 국내 호중구감소증 환자에서의 teicoplanin의 적정 용량, 용법 등을 알기 위한 집단 약동학 등의 연구를 시행할 예정이다. Background : This study was done to elucidate the efficacy of teicoplanin as the empirical treatment for febrile neutropenia. Methods : Patients were randomized to two groups according to pharmaceutical company (company A or B). Total of 49 patients (A, 27; B, 22) with neutropenic fever were studied prospectively for 6 months (Jul. 2003-Dec. 2003). Patients received 400 mg i.v. once, then 200 mg i.v. once daily. Results : Groups were matched for all demographic variables. Most of the patients were concurrently receiving nephrotoxic drugs. Gram positive microorganisms were isolated in 8 patients for A and 7 patients for B. Resistance rate against teicoplanin was 22.2% in A and 28.6% in B (P=1.0; 0.61 < 95% confidence interval [Cl] < 1.95). Among the patients with microbiologically documented infection, clinical cure or improvement was seen in 4 (50%) of 8 patients for A and 4 (57.1%) of 7 patients for B (P=1.00; 0.29 <95%CI <2.60). Bacteriologic efficacy was assessed as follows; elimination in 5 (55.6%), elimination with relapse in 2 (22.2%), resistance in 2 (22.2%) out of 9 gram-positive bacteria for A and 5 (51.4%), 0 (0.0%), 2 (28.6%) out of 7 bacteria for B, respectively (P=Q.28). There were no significant differences in duration of fever, duration of use of teicoplanin, and overall mortality. The incidence of nephrotoxicity and ototoxicity was not significant. Conclusion : For using teicoplanin as the empirical therapy for febrile neutropenia, the rate of clinical, microbiological response, and nephrotoxicity was 53.3%, 62.5%, and 16.3% respectively with no significant differences between the 2 preparations of teicoplanin. Supplementary evaluation on the adequate dose and duration of teicoplanin may be required.

Lidocaine, Thrombin, Epinephrine 의 항균효과

김진우,이동건,전혜선,김승준,김석찬,안중현,김치홍,권순석,김영균,김관형,문화식,신완식,송정섭,박성학 대한감염학회 2005 감염과 화학요법 Vol.37 No.6

목적 : 기관지내시경 검사에서 흔히 사용되는 국소마취제인 lidocaine과 내시경시 지혈목적으로 사용되는 thrombin과 epinephrine이 각종 균주에 미치는 항균효과에 대해 알아보고자 하였다. 재료 및 방법 : 균주는 가톨릭대학교 성모병원에서 2004년 3월부터 2004년 9월까지 임상검체 에서 동정된 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 각각 42, 42, 42, 43주를 대상으로 하였다. Lidocaine, thrombin, epinephrine 감수성 검사는 National Committee for Clinical Laboratory Standards (NCCLS, 2002)의 기준에 따랐다. 결과 : Lidocaine은 S. aureus, S. pneumoniae, P. aeruginosa에서 MIC_(50), MIC_(90) 모두 20,000 ㎍/mL 이었다. K. pneumoniae는 각각 10,000 ㎍/mL이었다. Thrombin은 S. aureus와 P. aeruginosa에서 MIC50 500 lU/mL 과 MIC_(90) 500 IU/mL 이상이었고, K. pneumoniae에서는 MIC_(50)과 MIC_(90)이 모두 500 lU/mL이상이었으나 S. pneumoniae에서는 MIC_(50)과 MIC_(90)은 125 IU/mL이었다. Epinephrine은 K. pneumoniae, S. pneumoniae에 대한 MIC_(50), MIC_(90)가 모두 >500 ㎍/mL이었고, S. aureus와 P. aeruginosa에 대한 MIC_(50), MIC_(90)가 모두 500 ㎍/mL이었다. 결론 : 기관지 내시경 검사에서 흔히 쓰이는 lidocaine, thrombin, epinephrine 등의 약제들이 호흡기 질환의 흔한 병원균인 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 균주들에 대해 항균 효과가 있을 수 있어 세균배양검사에 영향을 미칠 수 있겠다. Background : We performed this stody to find out about antimicrobial effect of lidocaine which is commonly used local anesthetic, and thrombin and epinephrine used for hemostasis during bronchoscopic procedures. Materials and Methods : The microorganisms that were cultured from specimens obtained during bronchoscopy were Staphylococcus aureus (n=42), Streptococcus pneumoniae (n=42), Klebsiella pneumoniae (n=42), and Pseudomonas aeruginosa (n=43) collected from St. Mary's Hospital, from March to Sep 2004 were used for susceptibity testing. Susceptibility to lidocaine, thrombin, and epinephrine were tested according to the National Committee for Clinical Laboratory Standards. Result : MIC_(50) and MIC_(90) of lidocaine for S. aureus, S. pneumoniae, P. aeruginosa were all 20,000 ㎍/mL and that for K. pneumoniae were 10,000 ㎍/mL. MIC_(50) and MIC_(90) of thrombin for both S. aureus and P. aeruginosa was 500 IU/mL and above 500 IU/mL, respectively; that for K. pneumoniae were all above 500 IU/mL and for S. pneumoniae they were 125 IU/mL, MIC_(50) and MIC_(90) of epinephrine for K. pneumoniae and S. pneumoniae were above 500 ㎍/mL; that for S. aureus and P. aeruginosa were 500 ㎍/mL. Conclusion : We observed possible antimicrobial effect of lidocaine, thrombin, and epinephrine in vitro against pathogens such as S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa, which are common respiratory microorganisms. The use of these agants could affect the result of bacterial culture.

환기정도에 따른 수술실용 신발 종류가 수술실 오염에 미치는 영향 : How to Choose Suitable Shoes?

남경동,정혜선,박영신,원진희,주미자,성화신,이지혜,이병희,조경숙,배재춘 한국의료QA학회 2000 한국의료질향상학회지 Vol.7 No.1

Background : Various types of protective footwear have been used to minimize bacterial contamination in operating rooms. In recent years, debate has arisen concerning the need for use of such protective footwear. This study was designed to provide useful data about choosing shoes most suitable for the surgical environment. Methods : Between November, 1999 and January, 2000, we performed this experimental study by comparing effect of three types of shoes (i.e., disposable shoescover, operating room-restricted shoes, and ordinary shoes) on bacterial contamination of operating rooms equipped with two different ventilation systems (i.e., high air-change, low air-change) respectively. Data were collected during two-hour sham operations in which subjects and their activities were strictly standardized. Bacterial flora were sampled from the study area floor and air colony-counts were measured. Results : In experiments involving high air-change ventilation system, there was a significant difference of floor contamination between three types of shoes, but no difference of air contamination. Under low air-change system, there was a significant difference of both floor and air contamination between three types of shoes. Conclusion : The results show that protective footwear would be unnecessary in the operating room with high air-change ventilation system, but it is important to choose suitable shoes carefully under low air-change system. Therefore, the use of outdoor shoes can be considered under high air-change system, but it would seem sensible to apply their first use in less bloody operations at the day surgery center or out-patient department to prevent transfer of body fluid into the outside environment.

울산 지역에서 대기중 벤젠으로 인한 암 사망 손실비용 추정 모형에 관한 연구

이용진,김예신,신동천,신영철 한국환경경제학회 한국자원경제학회 2004 자원·환경경제연구 Vol.13 No.1

울산 지역에서 대기중 벤젠으로 인한 발암 인체영향에 대한 경제성 평가를 위해서, 건강 위해성 평가를 수행하여 대상 지역의 이론적 암 사망지수를 추정하고, 대상 지역 주민을 대상으로 가상 가치평가법을 통한 위해도 감소의 지불의사금액을 추정하여 1인의 통계적 생명가치액을 유도하여 이론적 암 사망지수에 대한 사망 손실비용을 유도하였다. 대상 지역의 대기중 벤젠의 평균 농도는 7.88㎍/㎥(min : 1.16∼max : 23.32㎍/㎥)로 조사되어 US EPA IRIS의 벤젠 단위 위해도 2.2×10^(-6)∼7.8∼10^(-6)을 사용하고, 30세 이상의 노출인구수 51만 6,641명에 대한 인구 집단 위해도를 추정한 결과 10년간 발생 가능한 발암 사망지수는 2.90명(5 percentile : 0.32명∼95 percentile : 9.11명)으로 조사되었다. 1인의 통계적 생명가치액을 추정하기 위하여, 공단 지역의 지불의사금약 모형을 추정한 결과, 응답자의 암 치료경험 유·무와 기대 여명, 가족수, 교육수즌이 유의한 영향 요인으로 조사되었고, 연간 1만 평당 5명의 위해도 감소에 대한 지불의사금액은 월1만 4,852원(95% C.I : 1만3,135원∼1만6,794)으로 산출되었으며, 위해도 감소치를 고려한 1인의 통계적 생명가치액은 약 3억6,000만원(95% C.I:3억2,000만원∼4억원)으로 추정되었다. 대기중 벤젠의 발암 영향으로 인한 건강 손실비용은 발암 위해성 평가로 추정된 이론적 사망지수에 1인의 통계적 생명가치액을 곱하여 추정하였다. 추정 결과, 현 수준의 벤젠의 발암 영향이 10년간 지속될 경우 약 10억원(5 percentile: 1억원∼95 percentile: 33억원)의 건강 손실이 있는 것으로 추정되었다. 본 연구 결과, 대기중 벤젠의 노출로 인한 위해도를 줄이기 위해서는 벤젠 단독물질에 대해서 이론적으로 10년간 약 10억원에 대응하는 감소 대책에 투자하여햐 한다. 그러나 벤젠 한 물질만을 감소시키기 위한 대안은 현실적이지 못하므로, 이 연구를 모델로 하여 차후 벤젠을 포함한 휘발성 유기오염물질에 대한 총합 적용이 필요하고, 손실비용을 재추정해야 할 것이다. This study aimed to evaluate cancer damage cost due to risk from benzene inhalation. We performed health risk assessment based on US EPA guideline to estimate annual population risk in Ulsan metropolitan city. Also, we estimated a willingness-to-pay amount for reducing a cancer mortality rate to evaluate a value of statistical life. We combined the annual population risk and the value of statistical life to calculate the cancer damage cost. In the health risk assessment, we applied the benzene unit risk (2.2×10^(-6)~7.8×10^(-6)) in the US EPA's Integrated Risk Information System to assess the annual population risk. Average concentration of benzene in ambient air is 7.88 ㎍/㎥(min: 1.16~ max: 23.32 ㎍/㎥). We targeted an exposure population of 516,641 persons who aged over 30 years old. Using a Monte-Carlo simulation for uncertainty analysis, we evaluated that the population risk of benzene during ten years in Ulsan city is 2.90 persons (5 percentile: 0.32 ~ 95 percentile: 9.11 persons). And the monthly averagc WTP for 5/1,000 cancer mortality reduction during ten years is 14,852 Won(95% C.I: 13,135 ~ 16,794 Won) and the implied VSL is 36 million Won(95% C.I: 32~40 million Won). Cancer damage cost due to risk from benzene inhalation during 10 years in Ulsan city is about 104 million Won(5 percentile: 13 ~95 percentile: 328 million Won). Health benefit cost to reduce a cancer mortality risk of benzene is about 50 million Won in Ulsan metropolitan city. But, it is very important that this cost is not for all health damage cost of cancer mortality in some area. We just recommended a model for evaluating a cancer risk reduction, so we must re-evaluate all integrated application of total VOCs damage cost including benzene.