大豆幼植物切片의 組織培養에 의한 再分化

高永玉 진주산업대학교 농업기술연구소 1988 農業技術硏究所報 Vol.1 No.-

大豆幼植物切片을 材料로 여러 가지 培地條件에서의 組織培養으로 再分化의 方向을 提示코자 하였다. picloram과 BAP가 첨가된 배지에서 상배축조직의 칼루스형성이 가장 유리하였다. 이때 뿌리분화는 대체로 용이하였으나 shoot의 분화는 자엽마디에서만 이루어졌다. picloran과 BAP가 첨가된 배지에서 형성시킨 칼루스는 kinetin과 2.4-D가 첨가된 배지에 계대 배양하였을때 배양체와 조직학상의 밀집된 세포층을 확인할 수 있어 재분화가 가능한 것으로 기대되었다. A tissue culture experiment to determine a routine regeneration procedure in soybean has been conducted. When callus formed on MS medium supplemented with 0.01mg/l picloram plus 0.2 mg/l BAP were placed on MS medium supplemented with 2.0mg/l kinetin plus 1.0mg/l 2.4-D., differentiated tissues were developed. Histological studies of these cultures confirmed the organogenic nature of the cultures.

재식시 종근본수 차이가 감초 생육 및 수량에 미치는 영향

高永玉 진주산업대학교 1975 論文集 Vol.13 No.-

본 시험은 감초 재배기술 개선의 기초자료를 얻고져 진주농전 특작포장에서 Glycyrrhiza glabra L. 품종을 선택하여 1975년부터 정식시 종근본수를 5개수준 30본 35본, 40본, 45본, 50본으로 처리하여 이들 처리가 생육 및 수량에 미치는 영향을 검토한 바 그 결과를 요약하면 다음과 같다. 1. 생육조사 초장은 처리간에 차를 볼 수 없고, 포기당 분지수. 총 포기수는 정식시 종근 본수가 많을수록 많았다. 2. 수량조사 수량은 50본구가 제일 많으며 다음으로 종근본수가 많은 순이였으며 서로간에 고도의 유의성이 인정되었다. 근직경은 처리간에 차가 없고, 근장은 종근본수가 많을수록 길었다. These tests Carried out in order to get The fundamental date of improvement in technical Know-how for growing of Glycyrrhiza Globra L and is performed the planting seed-root an a unit of five(30.35. 40.45.50 planting seed-root in a unit of pot) at the bract in our School. 1975. In brief as a result of thes tests are: 1) A suvey of growing: there is no difference in a leng th of grasses. 2) A suvey of guantity: the most of guantity is in the unit of 50 pot the ranking is the more highly the planting see-root much become the numlrer.

Beanfly(Melanogromyza SP.) 被害率에 關한 硏究 : 第Ⅱ報 播種時期別·土壤殺蟲劑 處理別에 對한 大豆 被害調査 Ⅱ. An Investigation of the Beanfly Damage in Soybean According to Seedling Dates and Soil Insectcides

高永玉 진주산업대학교 1981 論文集 Vol.19 No.-

大豆의 害蟲인 Beanfly의 正確한 被害狀況을 把握코져 品種 水原―85, YBL―47, 播種日 5월 1일부터 10回 間隔으로 9回, 土壤殺蟲劑를 處理 無處理하여 栽培하고 Beanfly의 發生消長과 被害率을 調査한 結果를 要約하면 다음과 같다. 1. 播種日에 따라서는 5月 中旬과 7月 中旬에 각각 2번 Peak를 이룬다. 2. 殺蟲劑 處理間 品種間에는 差가 적었으며 이는 氣候의 영향으로 본다. In order to find out the exact beanfly damdge in soybean, an investigation on responses of soybean varieties(Su Weon-85, YBL-47), seeding dates(May 1, May 10, May 20, Jun. 1, Jun. 10, Jun.20, Jul. 1, Jul. 10, Jul. 20) and soil insectcides(treatment, nontreatment)to fluctuation of the beanfly and ratio of damage was carried. The results obtained in an investigation were summarized as follows: 1. Two peaks of the seasonal fluctuation of beanfly were shown by occurring on May 20 and July 10 during the soybean cultivation. 2. The beanfly damage was show low by soil insectcides, seeding dates and soybean varities. I think this situation was influenced by the weather.

오이 誘引 方法이 收量에 미치는 影響

高永玉,姜鎬允 진주산업대학교 1968 論文集 Vol.2 No.-

오이의 收量을 높이기 위하여 誘引方法과 植付??離 달리하여 調査를 하였다. 品種은 靑長마디 오이로 3月 5日에 播種, 54日苗를 合掌式(90∼45㎝) 晋州式(99×30㎝)으로 栽培을 하여 收量을 調査한 결과는 다음과 같다. 1.1株間에는 生育과 收量間에 有意差가 없었다. 2.區當收量에는 高度의 有意差를 볼 수 있다. 3.經濟性은 晋州式이 合掌式보다 倍 정도의 收入을 볼 수 있다. This experiment was caried out to get more yields of cucumber by the change of cucumber invitation and the intervals of plantation cucumber variety, as the material used in this experiment was Chung-Chang-Madi cucumber. Seed-sowing was done on fifth march and setting was done on fifth March and setting was done on twentynineth. Plant density was classified into two kinds, 90×44㎝ as the Hapchang-sik method, 99×30㎝ as the Chinju-sik method. The results obtained in this experiments were as follows. 1.Between individual spacing was not convinced significant differences. 2.There was high significant differences between the yields per plots. 3.According to economic values, the income of Chinju-sik was about two times than Hapchang-sik.

야생차 가공 기술 개발

고영옥,이종옥,신동국 진주산업대학교 농업기술연구소 2002 農業技術硏究所報 Vol.15 No.-

현대인의 기호에 맞는 지리산 반발효차(구황차)를 개발하기 위하여 옛날 제차법이 보존되어 있는 절간의 기술에 새로운 과학기법을 접목하여 개발한 차의 결과는 다음과 같다. 1. 총질소함량은 고온황토방의 녹차에 비하여 황토방의 녹차의 함량이 다소 높았다. 2. 총아미노산, 카페인 및 비타민-C함량은 황토방과 고온황토방의 제차방법에 따른 차이는 그다지 높지 않았다. 3. Geraniol함량은 황토방의 녹차(2회차)가 2.4%이며, 고온황토방의 녹차에 비하여 높은 경향이었다. 4. Pyrrole류의 pyrrole성분은 고온황토방이 다소 높았으나, ethyl pyrrole는 황토방에서 조성비가 현저히 높았다. 5. 꽃향기에 기여하는 Jasmone함량은 고온황토방의 녹차에서 조성비가 다소 높았다. In the order to taste go well with half-ferment green tea(kuhwang green tea) in the products of Mt. jiri garden were made try to development for modern people, according to once upon time there buddhist temple preserve course of green tea processing was approachable to new science of skill . The results were summarized as follows: 1. The amount of total nitrogen in green tea was high for yellow soil room than high temperature of yellow soil room 2. The amounts of total amino acid, caffeine and Vit. C in green tea were not difference according to yellow soil according to making method 3. The amount of geraniol in green tea was high tendency of yellow soil room(2.4%) than high temperature of yellow soil room 4. The component of pyrrole, in green tea was a few high such as high temperature yellow soil room, but the ethyl pyrrole was high ratio with yellow soil room. 5. The aroma component of amount of jasmone in green tea was a few high with high temperature of yellow soil room

牧丹(Moutan Cortex Radicis)의 번식에 관한 연구

高泳玉 진주산업대학교 1977 論文集 Vol.15 No.-

목단의 번식 종류에 따라 생리현상을 알고져 분주법, 접목법(공대접목, 이대접목) 실생법을 실시하여 조사한 결과를 보면 다음과 같다. 뿌리수, 뿌리직경, 가지수 모두 분주묘가 가장 우세하며 다음으로 공대접목묘, 실생묘가 우세하였다. 이대접목묘는 세력이 가장 나쁘나 화예용으로는 좋은 것 같다. Experiment were Carried out order to investigate physioliogical state of tree paeonia. The material were classified three kind of reproduction, division seedling, grafting, and seedlings. The results are summarijed as follows. 1. The number of root, root diameter and branch number of division seedling were more vivid than the stoick of tree peonia × the scion of the tree peony and The stock of peonia × the scion of peony. 2. The seedling of the stock of peonia × the scion of peony were not good hand ofter seedlings but lt is very good that we use blowering plant.

파종시기가 들깨 수량에 미치는 영향

高永玉 진주산업대학교 1971 論文集 Vol.8 No.-

Experiments were Carried out to make clear The effects on some perillal (South district) Characters by the changes of sowing date. The results obtained are sumarized as follows : The results in these experiments showed that in most cases maximum yields were obtained whe Kyung Nam local Variety, were shown in Mid-may, and progressively lower yields were produced Mid-April sowings.

무우, 배추 播種時期가 收量에 미치는 影響

高永玉,姜鎬允 진주산업대학교 1969 論文集 Vol.3 No.-

南部地方(晋州)에서 秋播 무우. 배추 栽培에 適合한 播種期를 調査하여 增産의 機構를 究明코져 무우(궁중총대. 서울무우)를 8月 20日. 8月 24日. 8月 28日. 9月 1日 月 5日로 배추(불암 1호)를 8月 9日. 8月 13日. 8月 17日. 8月 22日. 8月 26日. 황옆직예를 8月 19日. 8月 23日. 8月 27日. 9月 1日. 9月 5日로 各各 4日 間隔으로 5回 播種하였다 1. 배추播種適期는 結珠種(불암 1호)은 8月 10日∼8月 15日이며 半結珠種(황옆직예)은 8月 10日∼8月 30日 適當하다. 2. 무우 播種適期는 品種에 따라 差이가 있으나 서울무우. 궁중총대는 8月 20日∼25日이 收量이 많았다. Experiment were carried out to detect clear the effects on some radish & chinese cabbage characters by the change of seeding date in southern part of Korea(Chinju). Radish varieties used as the material were Goong Joong, Chang-tae, Seoul radish chinese cabbage varieties used as Bul-AmNo.1, Hwang Yub Jik-Ye(yellow leaf). Radish & chinese cabbage seed sowing was conducted at 5 times, 20th August, 24th August, 28th August, 1st September and 5th September. The other was 9th August, 13th Aught, 17th August, 22th August, and 26th August(Bil-Am No.1). 19th August, 23th August, 27th August, 1st September, and 5th September, (Hwang Yub jek-ye) at 4day-interval, 1968. Chinju Agricultural and Forestry Junior Technical College Experiment farm. The results of obtained are summarized as follows. 1. In southern part of Korea(Chinju) yield of radish which were seed on 10th, August to 15th August gave best results. And Hwang-yub jik-Ye was 10th August to 30th August. 2. The optimum seeding date fov radish seems to be around Sept. 20∼25.

自然農業에 관한 硏究 : 1. 自然農業 農家 土壤의 理化學的 硏究 1. The Physicochemical Study on Soil of Natural Agriculture Farmhaus

高永玉,小山敦史 진주산업대학교 농업기술연구소 1996 農業技術硏究所報 Vol.9 No.-

환경의 오염으로 고품질 농산물 생산의 필요도가 높아가고 있는 이러한 시대적 상황에서 환경보전형 농업인 자연농업 농가의 토양조사가 실시되어야 된다고 본다. 영·호남지역의 자연농업 농가토양의 이화학적 상황은 다음과 같다. 1. 토양내 미생물 조사 미생물은 작물생육에 영향이 많은 총세균, 콩근류근, 방선균, 사상균을 조사했는데 지역간에는 경주시에 가장 많았고 구미시에 적었다. 처리간에는 일반농가보다 자연농업농가에 많았다. 무경운토양보다 자연농업농가에 많았다. 2. 토양내 일반분석 조사 일반분석 상황은 표준량보다 많았는데 그중 인산이 제일 많았으며 칼슘은 약간 적었다. I think there should be a Soil Investigation of Nature Agriculture Farmhouse which is the Environment Conservated Farming, because nowadays, the necessity of high quality farmcrops is increasing from the pollution of an environment. Nature Agriculture Farmhouse soil of physics and chemistry's situation in Young, Ho-Nam region is as follows : A. The Investigation of Microorganism in Soil : I investigation that the growth of influenced microorganism is such as Total Bacteria, Bean Rhizobium, Actinimy Cetes, and Filamentous Bacteria. a. Region : Kyong-Ju city had the most followings in the above ; Ku-Mi city had the least. b. Handing : the nature Agriculture Farmhouse had much than the Common Farmhouse. c. The Nature Agriculture Farmhouse had much than the Non-Bounding Soil. B. The Common Analysis of Investigation in Soil : The Common Analysis had much than the amount of standard : among these, phosphoric acid was the most numerous, and Ca was little less.

大豆(Glycine max L.)組織培養과 그 遺傳에 관한 試驗

高永玉 진주산업대학교 1992 論文集 Vol.30 No.-

大豆 組織培養中 遺傳關係를 조사코져 根分化能이 좋은 突然變異系統 Mut-54와 原品種 CB-27를 상호교배시켜, 이들 培養中 callus 形成과 根分化能을 조사하였다. 배지는 MS 기본배지에 0.01㎎/ℓ picloram, 0.02㎎/g BAP를 각각 사용하였다. 1)상호교배한 F_l의 Callus 생장은 兩親에비하여 양호함. 2)繼代培養 하지 않고 그대로 두었을 때 兩親은 생장정지, 고사하였다. 3)F_l 根分化形質은 양친의 中間形質로 나타냈으며 상호교배에서도 비슷한 경향을 보였다. 4)F_2 Callus로부터 根分化는 原種 F_l Mut-54의 型이 1:2:1로 분리되었다. The objectives of the study are to examine heredity, Callus formation and capability for rooting in the Tissue culture of Glycime max by interbreeding and Cultivating mutant lines Mut-54 and mother variety CB-27, where 0.01㎎/ℓ Picloram and 0.02 ㎎/ℓ BAP on the MS basic media respectively. The results obtained were as follows; ① The growth rate of F_1 callus interbreeded was higher them that of F_2° ② F_1 passaged culture-ed was blighted. ③ The root differential character of F_1 was identifed as the median character of F_1 and was alike in the interbreed ④ The Type(specialization rate) of F.Mut-54 in the root differential from the callus of F_2 was separated by 1:2:1.