안구적출에 따른 위둔덕의 Calbindin D-28k과 c-fos의 변화에 관한 연구

김명,김기훈,김주영,하현철,안명수,김장민,조향훈,정명섭,장인엽 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Changes of Calbindin D-28k- and c-fos-immunoreactivities in the superior collicuclus after Eye Enucleation. Objective and methods: Calcium-binding proteins (CBPs) play an important role in the protection, differentiation and reorganization of the central nervous system. The effects of neonatal retinal deafferentation on a CBPs, calbindin D-28k were examined immunohistochemically in the superficial layer of the rat superior colliculus. Also early gene familly c-fos was examined to evaluate the neuronal characteristics in the superior colliculus after monocular enucleation. Results: On the experimental side of superior colliculus, the number of calbindin D-28k-immunoreactive (IR) cells was reduced (77.4% compared to control), but not fibers. Appearance of c-fos-like immunoreactivity was represented much more in the ipsilateral superior colliculus than contralateral side within 24h after eye enucleation. Conclusion: These results suggest that the changes of retinotectal projection may alter the expressional pattern of calbindin D-28k and c-fos expression.

High efficient rice transformation method by modifying co-cultivation conditions

Hyang-Mi Park,Yul-Ho Kim,Tae-Young Hwang,Chang In Yang,Ji Ung Jeung,Young Chan Cho,Kyoung Ho Kang,Chung-Kon Kim 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

Rice transformation method using A. tumefaciens has already been widely used to generate transgenic plants, the transformation rate is still low in most Korean elite cultivars. We made several modifications of the standard protocol especially in the co-cultivation step to improve the efficiency of the rice transformation. The co-culture medium was modified by the addition of three antioxidant compounds (10.5㎎/ℓ L-cysteine, 1mM sodium thiosulfate, 1mM dithiothreitol) and of Agrobacterium growth-inhibiting agent (5㎎/ℓ silver nitrate). Co-cultivation temperature (23. 5℃ for 1 day, 26.5℃ for 6 days) and duration (7 days) were also changed. The plasmid of pMJC-GB-GUS carrying the GUS reporter gene and the bar gene as the selectable marker was used to evaluate the efficiency of the transformation. After co-cultivation, a high level of GUS gene expression was observed in calli treated with the modified method. It is likely that those newly added compounds helped to minimize the damage due to oxidative bursts during plant cell-Agrobacterium interaction and to prevent necrosis of rice cells. And the transformation rate under the modified method was also remarkably increased approximately 8-fold in Heungnambyeo and 2-fold in Ilmibyeo as compared to the corresponding standard method. Furthermore, we could produce the transgenic plants stably from Ilpumbyeo which is a high-quality rice but its transformation rate is extremely low. Transformation and the copy number of transgenes were confirmed by PCR, bar strip and Southern blot analysis. The improved method would attribute reducing the effort and the time required to produce a large number of transgenic rice plants.

Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

백서의 복강내에 투여한 Mitomycin-C-Polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

송규상,강대영,최정목,노승무,정경수,오정연,김진향,양준목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.

특발성 과호산구 증후군 환자의 치료 도중 발생한 간혈종 1례

이영태,김동기,박지훈,김윤정,김기향,박보민,권민정,김애란,이원식,주영돈,손창학 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Idiopathic hypereosinophilic syndrome is characterized by marked elevation of eosinophil count ( >1,500/㎣) in peripheral blood without explaining etiology. It shows tissue involvement in many organs such as liver, G-I tract, myocar dium, lung, skin, kidney and nerve. Some case of hypereosinophilic syndrome with hepatic involvement have been reported, but not much. We have recently experienced a case with idiopathic hypereosinophilic syndrome of a 40 year old man who presented with RUQ pain. By abdominal CT, ill- defined subcapsular hematoma was detected. Liver biopsy showed eosinophilic infiltration within the portal tract and sinusoid. Drain procedure and corticosteroid adminiiatbrought marked improvement of clinical manifestation, hematologic abnormality and radiologic abnormality.

역위 매복된 상악 소구치의 Enamel matrix derivative를 이용한 자기이식 치험례 : A CASE REPORT

오유향,이난영,이창섭,이상호 大韓小兒齒科學會 2003 大韓小兒齒科學會誌 Vol.30 No.3

치아의 자가이식 시 실패의 가장 큰 요인으로는 치주인대의 괴사를 유발하는 외상을 들 수 있으며, 이러한 외상은 치근흡수와 유착을 일으킬 수 있다. 따라서 자가이식의 성공을 위해서는 이식치아를 외상없이 제거하는 것이 중요하고, 매복치아의 자가이식 시에 발생되는 광범위한 조직 결손에서 치주조직의 재생과 주위조직의 재건 또한 필요하다. 치주조직 재생을 도울 수 있는 재료 중 최근에 소개된 Enamel matrix derivative (EMD)는 amelogenin계열의 법랑기질단백을 포함하고 있으며 여러 연구에서 치주질환 치료에 매우 효과적이라고 보고되었고 치주인대 세포의 분화를 증진시키므로, 치아자가이식에 유용하게 사용될 수 있음이 제시되어왔다. 본 증례에서는 상악 우측 제2소구치의 미맹출을 주소로 내원한 14세의 여환아에서 역위 매복된 미성숙 치아에 EMD를 적용하여 자가이식을 시행하고 관찰한 결과 치근 흡수나 유착의 소견이 관찰되지 않았고 향상된 조직재생의 소견을 보였다. The success of autotransplantation depends on the viability of periodontal ligament in the transplanted tooth. Mechanical injury to periodontal tissues frequently results in dental root resorption and ankylosis, which leads to the failure of transplantation. Enamel matrix derivative(EMD) Which contains several enamel matrix protein (amelogenin family) has been reported to be effective in some periodontal therapies has been recently used to induce periodontal regeneration. EMD promotes proliferation of periodontal ligament cells and is suggested to be useful for transplantation. In this case, we report a clinical case of EMD application in the transplantation of an impacted and immature tooth of a 14 year-old girl to enhance the periodontal regeneration.

광중합 광원의 종류와 조사시간에 따른 치면열구전색제의 미반응 모노머 용출

오유향,박윤경,이난영,이창섭,이상호 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.3

본 연구에서는 기존의 할로겐 광과 보다 강한 강도와 짧은 조사시간을 갖는 아르곤 레이저와 플라즈마 광의 광중합도를 평가하기 위해 많은 양의 TEGDMA를 용출하는 치면열구전색제를 실험재료로 이용하여 각각의 중합시간과 용출시간에 따른 미반응 TEGDMA의 양을측정, 비교 분석하여 중합도를 평가하였다. 광원에 따른 중합시간 당 각각 10개의 시편을 제작하여 3차 증류수에 넣은 후 바로 용출시킨 액을 0시간으로 하고 37℃ 항온기에서 10분, 1시간, 12시간, 24시간 동안 용출시켰다. 각 용출액의 20 μl를 역상 크로마토그래피(RP-HPLC)에 적용시켜 미반응 모노머의 시간경과에 따른 용출양을 측정하였다. 이상의 실험을 통해 얻은 결과는 다음과 같다. 1. 할로겐 광은 중합시간이 증가할수록 TEGDMA의 초기 용출량 감소를 보였다(p>0.05). 2. 플라즈마 광은 6초, 9초로 중합한 군에서는 TEGDMA의 용출량의 차이를 보이지 않았으나, 3초 중합 군에서는 6초와 9초 중합 군보다 초기 용출량이 크게 나타났다(p<0.05). 3. 아르곤 레이저는 전반적으로 할로겐 광과 플라즈마 광보다 TEGDMA의 초기 용출량이 컸으며 조사시간이 증가할수록 용출량은 감소되었다 (p<0.05). 4. 광원의 제조회사에서 권장하는 최소 중합시간인 할로겐 광 20초, 플라즈마 광 3초, 아르곤 레이저 5초로 조사시 TEGDMA의 용출량은 할로겐 광. 플라즈마 광, 아르곤 레이저 순으로 용출량이 적었다(p>0.05). 5. 제조회사의 권장 중합시간인 할로겐 광 40초, 플라즈마 광 6초, 아르곤 레이저 10초 조사시 할로겐 광 40초와 플라즈마광의 6초의 TEGDMA의 용출량은 아르곤 레이저 보다 낮게 나타났다(p>0.05). 6. 권장시간 이상인 할로겐 광 60초와 플라즈마 광 9초, 아르곤 레이저 20초 중합시 나타나는 TEGDMA의 용출량 간에는 유의할만한 차이가 없었다(p>0.05). The purpose of this study was to measure and compare the amount of unreacted TEGDMA from pit and fissure sealants cured with three different light sources ; conventional halogen light curing unit, plasma arc light curing unit and argon laser The specimens were eluted in distilled water for different time intervals. The time-related release of TEGDMA were analyzed by reverse-phase high performance liquid chromatography(HPLC). The result of present study can be summarized as follows: 1. The time-related release of TEGDMA decreased with increasing curing time in conventional halogen light, however, that not statistically significant difference(p>0.05). 2. The elution from the specimens cured for 6 and 9 seconds with plasma arc light was similar results corresponding with the time-related TEGDMA release, and was significantly lower than that cured for 3 seconds(p<0.05). 3. The elution of TEGDMA from the specimens cured with argon laser was significantly higher than that cured with halogen and plasma arc light(p<0.05). 4. The elution of TEGBMA from under recommended time of three different light sources were showed to be no statistically significant difference(p>0.05). 5. In time-related release of TEGDMA from recommended time of each light sources, the results correspond to 40 seconds of halogen light and 6 seconds of plasma arc light were similar(p>0.05). 6. The elution of TECDMA from over recommended time of three different light sources were showed to be no statistically significant difference (p>0.05). In this study, I suggest that curing time of plasma arc light is 6 and/or 9 seconds in the field of clinical pediatric dentistry claiming its effectiveness in optimal polymerization and reduced chair time.

광중합기를 이용한 광학적 치아우식활성도 검사법

이난영,김미라,오유향,이창섭,이상호 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.4

레이저와 플라스마 광, 할로겐 광 중합기를 이용하여 각 개인의 우식 활성도를 측정할 수 있는지를 규명하기 위해 7-8세 아동 45명을 대상으로 치아의 순면과 협면에 각 광원을 조사하고 특수 필터를 사용하여 초기 치아우식증으로 판별된 치아의 개수를 측정하여 기존의 우식활성도 측정방법인 dDfFtT rate와 mutans streptococci 측정법 Lactobacilli 측정법과 비교, 평가하고 그 특이도, 민감도, 예측력을 평가하였다. 1. 아르곤 레이저, 플라스마 광, 할로겐 광 조사시 육안검사시 보다 유의하게 많은 초기우식 병소가 관찰되었다(p<0.05). 2. 아르곤 레이저, 플라스마 광, 할로겐 광을 이용한 우식활성도 측정법은 dDfFtT rate와 양의 상관관계(γ= 0.42, 0.41, 0.39)를 보였다(p<0.01). 3. 기존의 우식활성도 측정방법을 기준으로 하여 평가한 결과 특이도와 민감도, 예측력은 모두 레이저가 가장 높았으며 플라스마 광, 할로겐 광 순으로 나타났다. 이상의 결과를 종합하여 볼 때 아르곤 레이저와 플라스마 광, 할로겐 광을 이용한 형광법은 모두 양호한 진단학적 지표를 보여줌으로써 향후 광학적 우식활성검사법으로 활용 가능성이 높을 것으로 사료된다. The purpose of this study was to develop a practical caries activity test by fluorescence using laser, plasma light and halogen light. The subjects of study were 45 children of 7-8 years old. Argon laser, plasma light and halogen light were irradiated to buccal or labial surface of all teeth. Fluorescence of initial carious lesion from teeth was observed through barrier filter and the number of teeth showing lesion was counted. Visual examination for the dDfFtT, mutans streptococci screening test and Lactobacilli colony counting were also done. Data analysis was accomplished by Axelsson's method. The result from the present study can be summarized as follows. 1. Laser, plasma light and halogen light could detect the initial carious lesions better than visual examination(p<0.05). 2. There was positive correlation between laser(r=0.42), plasma light(r=0.41), halogen light(r=0.39) and dDfFtT rate(p<0.01). 3. The specificity, sensitivity and predictive value was showed highest value in laser. but was showed favorable value in plasma light and halogen light. In regard to above results, laser, plasma light and halogen light all considered to be reliable method for determining individual caries activity. And they were also considered to be practical method because it would be simple, inexpensive, and time saving method.

사용자 수술명과 수술분류 code(ICD-9-CM) 일치율 향상에 관한 연구 : 수술실 OCS program 사용 활성화를 통하여

최향하,김미영,김도진,유지원,장정화,박수정,박재성 한국의료QA학회 2005 한국의료질향상학회지 Vol.12 No.1

Background : The necessity of unify and standardize codes used at hospital has been emphasized since OCS (Order Communicating System) was adopted. Therefore, the purpose of this study were to standardize operation code by continuous training of the ICD-9-CM code that is used as standard code in OCS program at operating room. Method : In 400 operation data, operation code entered in OCS program at operating room was compared to operation name recorded in medical record. In addition, a matching rate between input data of operation code by medical record department and computing input data of operation code in 3,710 cases was compared for each department. User operation name and operation code were matched and major diagnosis by operation department and operation name were also matched. Results : User operation name was reflected in operation classification code in detail, and operation code entered on user was registered. Input rate and matching rate of operation code were gradually improved after improvement activity. In particular, a matching rate was high at ophthalmology where operation name is segmented. Plastic surgery and orthopedics with a lot of emergency operation and comprehensive operation name show low input rates. Conclusions : As the medical field makes progress in computerlization, awareness of information exchange and sharing becomes higher. Among codes to classified medical institution, codes related to surgical operation are all different by user of hospital and department. Computerlization and standardization is essential. And when efforts of standardization continue in alliance with individual hospital and institution, initiative of preparing medical policy data at a national level will be accelerated.

완속기시(Soft-start)광조사 방식이 복합레진의 중합 및 수축응력에 미치는 효과

위유민,오유향,이난영,이창섭,이상호 大韓小兒齒科學會 2005 大韓小兒齒科學會誌 Vol.32 No.2

본 연구에서는 기존의 할로겐 광중합기를 이용하여 40초간 조사하여 복합레진을 중합한 경우와 플라즈마 광중합기를 이용한 고강도의 중합 및 완속기시 중합 방식, 그리고 LED 광중합기를 이용한 통법의 중합과 완속기시 중합 방식으로 복합레진을 중합하여 발생되는 수축응력을 비교하고 미세경도를 측정하여 중합도를 평가하였다. 내경 7mm, 외경 10mm의 아크릴릭 주형을 제작하고 외면에 스트레인게이지를 부착 시킨 뒤 각각의 광원에 따른 중합모드로 광중합 하였다. 광조사 시점부터 1초 간격으로 600초간 수축응력을 측정하였으며, 중합 24시간 후 각 군의 미세경도를 측정하여 통계 분석하였다. 수축응력 측정 후 시편을 종단하여 주사전자현미경으로 레진수복물과 아크릴릭 주형 계면을 관찰하였다. 이상의 실험을 통해 얻은 결과는 다음과 같다. 1. 플라즈마 광과 LED 광원의 완속기시 중합방식은 각각의 기본 중합방식에 비해 중함 10분 후에 수축응력 감소를 보였다(P<0.05). 2. 완속기시 중합방식의 플라즈마 광이 가장 낮은 수축응력을 보였으나 미세경도 또한 가장 낮았다(P<0.05). 3. 완속기시 중합방식의 LED 광중합은 기존의 할로겐 광과 LED 광중합 방법에 비하여 낮은 수축응력을 보였다(P<0.05). 4. 완속기시 중하장식의 LED 광으로 조사한 시편의 미세경도는 단일광도로 조사한 할로겐 광과 LED 광중합과 비교하여 유의할만한 차이가 나타나지 않았다(P>0.05). 5. 기존의 할로겐 장과 완속기시 중합방식의 LED로 중합한 시편이 플라즈마 광과 단일강도의 LED로 조사한 군보다 더 나은 번연봉쇄를 보였다. The purpose of this study was to evaluate the influence of soft-start light curing on contraction stress and hardness of composite resin. Composite resin mold was cured using the one-step continuous curing method with three difference light sources; conventional halogen light curing for 40 seconds at 400 mw/㎠, plasma arc light curing for 6 seconds at 1300 mW/㎠ and LED light curing for 10 seconds at 700 mW/㎠. For the soft-start curing method 2 seconds light exposure at 650 mW/㎠ followed by 3 seconds at 1300 mW/㎠ and exponential increase with 5 seconds followed by 10 seconds at 700 mW/㎠ were used. Contraction stress was measured using strain gauge method and Vickers hardness was measured 24 hours after polymerization at the top and bottom of specimens. Resin-acrylic interfaces were observed using a scanning electron micro scope(SEM). The results of present study can be summarized as follows: 1. Contraction stresses at 10 min after polymerization were significantly reduced with the soft-start curing both in plasma and LED light sources(P<0.05). 2. Plasma light curing with soft-start resulted in not only the lowest contraction stress, but also the lowest hardness (P<0.05). 3. LED light curing with soft-start showed lower contraction stress than the one-step continuous halogen and LED light curing(P<0.05). 4. Micro hardness of specimens cured by LED light with soft-start was equivalent to that of cured by the one-step continuous halogen and LED light(P>0.05). 5. Curing by LED light with soft-start and conventional halogen light resulted in better marginal sealing than plasma light and one-step LED light curing.