http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
생쥐 대장 평활근 세포에서 내향 정류성 칼륨 전류의 특성 연구
이은주,김명,정명섭,조향훈,김기훈,하현철,김준수,최석,전제열 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.2
Objectives: K^(+) channels play an important role in regulating cellular excitability. The aim of this study was to know whether or not inward rectifier K^(+) channel exists in colonic smooth muscle cells. Methods: Mouse colonic smooth muscle cells were isolated using collagenase, and then we recorded their membrane currents using a whole-cell patch clamp technique. Results: With 90 mM K^(+) in bath, hyperpolarization-induced inward currents from -120 mV to 20 mV with 400 ms duration at a holding potential of -10 mV showed rapid activation, inactivation and inward rectification. The inactivation showed single exponential time course. Reduction of external K^(+) to 60 mM decreased the amplitudes of the currents in whole test voltage range and shifted the reversal potential to more negative potential. The inactivation process and peak currents of hyperpolarization-induced inward currents were not affected by removing external Na^(+). External Ba^(+) blocked hyperpolarization-induced inward currents by dose-dependent manner and pure Ba^(+)-sensitive currents showed strong inward rectification. Cs^(+) also suppressed hyper- polarization-induced inward currents. Ba^(+) and Cs^(+)-induced inhibitiOn of hyperpolarization-induced inward currents was voltage-dependent, and the extent of inhibition increasing with membrane hyperpolarization. Conclusions: These results suggest that inward rectifier KU channels may exist in proximal colonic smooth muscle and may play an important role in regulating membrane potential.
안구적출에 따른 위둔덕의 Calbindin D-28k과 c-fos의 변화에 관한 연구
김명,김기훈,김주영,하현철,안명수,김장민,조향훈,정명섭,장인엽 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2
Changes of Calbindin D-28k- and c-fos-immunoreactivities in the superior collicuclus after Eye Enucleation. Objective and methods: Calcium-binding proteins (CBPs) play an important role in the protection, differentiation and reorganization of the central nervous system. The effects of neonatal retinal deafferentation on a CBPs, calbindin D-28k were examined immunohistochemically in the superficial layer of the rat superior colliculus. Also early gene familly c-fos was examined to evaluate the neuronal characteristics in the superior colliculus after monocular enucleation. Results: On the experimental side of superior colliculus, the number of calbindin D-28k-immunoreactive (IR) cells was reduced (77.4% compared to control), but not fibers. Appearance of c-fos-like immunoreactivity was represented much more in the ipsilateral superior colliculus than contralateral side within 24h after eye enucleation. Conclusion: These results suggest that the changes of retinotectal projection may alter the expressional pattern of calbindin D-28k and c-fos expression.
생쥐 작은창자 사이질세포에서 bradykinin의 효과 및 bradykinin 수용체 아형의 발현
고영엽,전제열,최석,임용,김준수,문현식,김명룡,조경원,조향훈,장인엽 대한체질인류학회 2004 대한체질인류학회지 Vol.17 No.4
소화관 염증 시 소화관 운동성 이상이 초래되며 여기에 내인성 매개물질들이 관여하는 것으로 알려져 있 다. Bradykinin은 그 중 하나의 매개물질로서 신경전달물질이 아닌 펩티드성 국소 호르몬으로 염증이나 조직손상 시 혈장으로부터 생성되어 직접 민무늬근에 작용하여 소화관 운동성에 영향을 주고 있다. 소화관 운동성을 조절 하는 창자사이질세포(interstitial cells of Cajal, ICC)는 소화관 운동의 향도잡이 역할과 더불어 창자신경계로부터 민무늬근으로 신호를 매개하는 기능을 담당하고 있다. 본 연구는 생쥐 작은창자에서 배양된 창자사이질세포와 작 은창자근육층 전층표본을 대상으로 전기생리학적 및 면역조직화학적 방법을 통하여 bradykinin의 작용 및 분포를 알아보았다. Bradykinin-2는 창자사이질세포에서 세포막의 저분극을 유발하였으며, 소화관운동에 있어서 향도잡이 전류의 긴장성을 활성화 시켰다. 이중면역형광염색 결과, c-kit과 bradykinin-2 수용체가 창자사이질세포에서 함께 발현되었다. 따라서 본 실험의 결과 bradykinin이 간접적으로 창자사이질세포를 통하여 소화관 운동성을 조절할 수 있음을 시사하며 이 작용은 bradykinin-2 수용체를 통하여 매개되는 것으로 여겨진다. Interstitial Cells of Cajal (ICC) are pacemaker cells that generates slow waves and drive spontaneous mechanical contractions of gastrointestinal smooth muscle. Slow waves are generated the periodic activation of spontaneous inward currents (pacemaker currents). We studied the modulation of pacemaker activities by bradykinin (10-8 M) in cultured ICC with the whole cell patch-clamp technique, and the localization of bradykinin-2 receptor-immunoreactivity using double labelling immunohistochemistry in the murine small intestine. Externally applied bradykinin produced membrane depolarization in current-clamping mode. At a -70 mV of holding potential bradykinin increased tonic inward pacemaker currents. Double labelling with bradykinin-2 receptor and and c-kit was shown that ICC expressed the bradykinin-2 receptor-immunoreactivity. These results suggest that bradykinin modulates electrical activities of ICC via bradykinin-2 receptor, which may regulate gastrointestinal motility.