쥐와 개의 부신의 Somatostatin 분포에 대한 면역조직화학적 연구

문정석,정윤영,김준수 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

The adrenal medulla using immunohistochemistry few somatostatin immunoreactive nerve fibers were demonstrated in adrenal medulla, In addition, a large population of chromattin cells in the dog adrenal medulla displayed intense somatostatin-like immunoreactivity especially in the peripheral and perivascular region. About 10~20% of the adrenaline cells were the somatostatin-immunoreactive. The present study confirms the somatostatin is localized in the dog adrenal chromattin cell, and it is suggested that somatostatin may have a paracrine or endocrine role in the dog adrenal gland.

전경대원에서 발생한 결핵의 임상 양상

문창기,박상준,조민구,김영중,김소연,김윤권,정준오,안석진,김은실,서승오,김지훈,최원제,이윤영,박형기,최규영,김현근 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

Background: The purpose of this study was to document the incidence and clinical characteristics of patients with tuberculosis (TB) in combat and auxiliary police, living in a group, in Korea where the incidence rate of active TB in a general population is higher than in Western countries. Materials and Methods:We retrospectively reviewed the medical records of all tuberculosis patients diagnosed at National Police Hospital from January 2002 through December 2004. Results:In 2002-2004, a total of 156 cases of tuberculosis were identified with the mean (Standard deviation) age of 20.6 (±1.0) years. Of these, 134 (85.9%) patients were registered as new cases, 11 (7.1%) as relapse, 2 (1.3%) as failure, 5 (3.1%) as treatment-after-default cases whereas 4 (2.6 %) patients were not included in any categories. Average annual new TB rate and smear-positive TB rate were 86.5/10^(5) and 17.4/10^(5) person-years, respectively. In 12 of 31 smear-positive cases, time from onset of symptoms to diagnosis was more than 30 days. Two multidrug-resistance TB cases were identified and two suspected outbreak episodes of TB had occurred during 3 years. Conclusion:There was no statistically significant difference in the incidence rate of new cases of TB between the general population aged 20 to 29 years and combat and auxiliary police in Korea. 배경 : 국내 결핵의 발생은 감소 추세에 있으나 여전히 후진국형 발생양상을 보이고 있다. 특히 집단생활을 하는 젊은이들에서 발생하는 결핵의 유행이 간헐적으로 알려지고 있어 우려를 낳고 있다. 이에 저자들은 최근 전경대원의 결핵 발병 양상에 대해 알아보고자 본 연구를 시행하였다. 재료 및 방법 : 2002년 1월부터 2004년 12월까지 3년간 경찰병원에서 결핵 진료를 받은 전경대원의 의무기록을 후향적으로 분석하여 연도별 결핵 신환발생률, 도말양성결핵 신환율, 진단 지연, 집단발병, 다제내성결핵을 조사하였다. 결과 : 연도별 결핵 신환자 수(10만명당 발생추정치)는 2002년 50명(83.6명), 2003년 42명(83.7명), 2004년 42명(93.5명)이었고 도말양성 폐결핵 신환자 수는 2002년 10명(16.7명), 2003년 11명(21.9명), 2004년 6명(13.4명)이었다. 3년간 도말양성 폐결핵 환자는 31명이 있었고 그 중 12명은 호흡기 증상 발생 30일이 지나서 진단을 받았다. 다제내성 결핵은 2명이 있었으며 결핵의 집단발병이 의심되는 사례는 2차례 있었다. 결론 : 전경대원과 20-29세 연령군 일반인의 결핵 신환발생률, 도말양성 폐결핵 발생률의 통계적으로 유의한 차이는 없었다.

베타, 감마-불포화 케톤의 광화학에 미치는 히드록실기의 영향 : o-Hydroxyphenyl 1,2-Dimethylcyclopent-2-enyl Ketone의 합성 Synthesis of o-Hydroxyphenyl 1,2-Dimethylcyclopent-2-enyl Ketone

문종택,공영철,주동준 慶熙大學校 1997 論文集 Vol.26 No.-

To study the effect of the intramolecular hydrogen bondinghydroxyl group on the photochemistry of a β,γ-unsaturated ketone, racemic ο-hydroxyphenyl 1,2-dimethylcyclopent-2-enyl ketone and ο-methoxyphenyl 1,2-dimethylcyclopent-2-cnyl ketone were synthesized. Thus these two β,γ-unsaturated ketone were synthesized from ethyl 2-oxocyclopentanecarboxylate, after eight steps, in 18% yield.

Increased expression of P53 and Bax in the spinal cords of rats with experimental autoimmune encephalomylitis

문창종,김승준,위명복,김희석,정종태,박전홍,지영흔,Tanumab, Naoyuki,Matsumoto, Yoh,신태균 濟州大學校 農科大學 動物科學硏究所 2000 動物科學論叢 Vol.15 No.1

The expression of pro-apoptotic molecules, p53 and Bax, in the spinal cord of rats with experimental autoimmune encephalomyelitis (EAE) was examined. Apoptosis was confirmed by the terminal deoxynucleotidyl transferasemediated dUTP nick end-labeling (TUNEL) method. TUSEL (+) apoptotic cells were mainly either ED1 (+) macrophages or T-cells in the parenchyma of E N . Western blot analysis showed that both p53 and Bax expression significantly ( p < 0.01) increased in the spinal cords of EAE rats at the peak stage, and thereafter declined. An immunohistochemical study showed that inflammatory cells (notably T cells) in the parenchyma express p53 and Bax, while brain cells, includng neurons and glia, were devoid of these nuclear staining of these molecules. The nuclear expression of p53 largely matches apoptotic cells in the parenchyma of EAE. These finchngs suggest that pro-apoptotic molecules, p53 and Bax, may play an important role in eliminating T cells in the parenchyma in EAE.

양어용 사료첨가제로서 감귤발효액 (EM-Fermented Orange)의 항산화특성

문상욱,이영돈,이준백,고유봉 제주대학교 해양연구소 2000 해양자원연구소연구보고 Vol.24 No.-

Mandarine orange (Citrus unshiu Marc.) only added with sugar was fermented by effective microorganisms (EM). called "EM-fermented orange". EM was mainly composed of lactic acid bacteria. phototrophic bacteria and yeast. As pH of EM-fermented orange reached 3.5 to 4.0 in the course of fermentation. the incubation was stopped. and then. EM-fermented orange was preservable at room temperature for about 6 months. Composition and concentration of free amino acids in EM-fermented orange were analyzed. Concentration of total amino acids was 167 mg/l. and the relative content of carnosine (β-alanyl-L-histidine) which was well known as a kind of antioxidant materials was very high. 41%. In addition. crude extract showing antioxidant activity could be obtained from EM-fermented orange using some organic solvents. The antioxidant activity of the crude extract was equivalent to about 9% of that of vitamin E by the use of 1.1-diphenyl-2-picrylhydrazyl (DPPH) method. It was suggested that EM-fermented orange having antioxidant characteristics could promote the health of fish.

제주 차귀도 연안해역 미생물 분포 및 이화학적 특성

문영건,김만철,이준백,송춘복,여인규,김종만,박근태,손홍주,최우봉,허문수 한국환경과학회 2006 한국환경과학회지 Vol.15 No.3

公共體育施設의 效果的인 活用方案

李永熙,金顯俊,鄭升溶,李揆文 忠北大學校 平生體育硏究所 1987 平生體育硏究所 論文集 Vol.2 No.-

In order to check and develop an effective use of public physical facilities. We have consulted the statistical data, the concerned officials from countries, cities and provinces, and the questionnaire. Then we have analized the nation's present situation of physical facilities according to their founders, kinds, and localities, and thereby we obtained the following results and problems abot the situation of management and the use of physical facilities in Chungbuk province: 1. The nation and the local government should open the public physical facilities to the people of the community and increase the percentage of their users by enlarging the lacking facilities as needed by the community. 2. It is necessary to support the physical activities of people in the community by stationing a leader in the facilities, organizing clubs, and developing programs. 3. It is necessary to induce people to take active part in the physical activities by publicizing the method of using the facilities, organizations, programs, and detailed activities. 4. The public facilities should be equipped with the attendant things and also controled by an employed manager. 5. Financial support should be continued till the management of the facilities is self-dependant and activated. 6. The use of physical facilities smould contribute to the health and physical abilities of people and the love of the facilities and nation.

월성원자력발전소 주변의 삼림식생 분석

이호준,김인택,정흥락,전영문,윤영진,임웅규 建國大學校基礎科學硏究所 1996 理學論集 Vol.21 No.-

월성원자력발전소 주변의 삼림식생을 분석하기 위하여 1994년 4월부터 1994년 10월까지 식물사회학적 조사를 실시하였다. 또한 상관에 의해 현존식생도 및 녹지자연도를 작성하였으며, 순 1차 생산력과 식물현존량도 추정하였다. 군집조성표에 의한 삼림군락은 소나무군락, 곰솔군락, 굴참나무군락, 상수리 나무군락의 4군락으로 구분되었다. 각 군락의 주요 구성종으로는 소나무군락에서 소나무, 졸참나무, 털진달래, 맑은대쑥, 산거울, 곰솔군락에서 곰솔, 소나무, 진달래, 청미래덩굴, 굴참나무군락에서 굴참나무, 조록싸리, 큰기름새, 맑은대쑥, 댕댕이덩굴, 상수리나무군락에서 상수리나무, 갈참나무, 큼기름새, 그늘사초, 맑은대쑥 등이다. 현존식생도에 의한 삼림군락의 분포율은 곰솔군락이 40.3%로 가장 높고, 녹지자연도는 7등급이 60.0%로 가장 넓은 분포율을 보였다. 삼림토양의 pH는 4.45∼5.32, 유기물함량은 5.61∼6.34%의 범위로 나타났다. Montreal model에 의한 순 1차 생산력은 1,555.2g/㎡/yr, 식물현존량은 14,963.32ton/㎢로 추정되었다 The forest vegetation, estimation of phytomass and net primary production on the vicinity of Wolsong Atomic Power Plant were analyzed on the basis of the phytosociological data. And depending on physiognomy, actual vegetation map and the degree of green naturality(DGN) was drawn in 1:50,000 scale. According to Z-M method, the forest vegetation was classified into 4 communities; A. Pinus densiflora community, B. Pinus thunbergii community, C. Quercus variabilis community, D. Quercus acutissima community. Generally, Pinus densiflora, Quercus variabilis, in tree-1 layer, Quercus acutissima, Quercus variabilis, in tree-2 layer, Rhodedendron mucronulatum, Quercus serrate, in shrub layer, Artemisia keiskeana, Carex humilis, in herb layer, showed high coverage index. The pH and contents of organic matter of the forest soil collected in each sites were shown range of 4.45 to 5.32 and 5.61% to 6.34% in mean of communities respectively. The distribution rate in actual vegetation map were the highest in Pinus thunbergii community. The average DGN showed to 5.1. Net primary production and phytomass per unit area which estimated by Montreal model were assumed in 1,555.2g/㎡/yr, 14,963.32ton/㎢, respectively.

심상성 루프스에서 Nested-Primer Gene Amplification Assay에 의한 결핵균 DNA의 검출

신문석,전효진,김병천,이규석,김재룡,권호준,송준영 啓明大學校 醫科大學 1994 계명의대학술지 Vol.13 No.4

Lupus vulgaris, which is a progressive form of postprimary tuberculosis in patients with a moderate to high degree of immunity and tuberculin sensitivity, is the most common, most serious, and most variable type of cutaneous and mucous membrane tuberculosis. Domonstration of M.tuberculosis directly or in culture in some of these eruptions can be difficult. It is essential to demonstrate the presence of M.tuberculosis in a clinical sample for a definitive diagnosis of tuberculosis. With the discovery of the polymerase chain reaction (PCR), the DNA diagnostic approach for infection has developed in a short period. With the dual purpose of definitive and rapid diagnosis of tuberculosis, this study examined the usefulness of a nested PCR for detection of M. turberculosis. Compared with a single-step PCR, the two-step PCR was able to enhance sensitivity approximately 1,000-fold on the basis of bacterial counts and DNA quantity. We concluded that two-step nested PCR appeared to be the most useful PCR protocol for early and sensitive diagnostic method detecting M.tuberculosis in acid-fast stain negative specimens.

플러그셀 크기 및 용토가 거어베라의 묘생장에 미치는 영향

조문수,예병쾌,박윤영,전하준 한국환경농학회 2003 한국환경농학회지 Vol.22 No.1

양액재배용 거어베라 우량묘를 생산하기 위한 기초연구로 플러그셀 크기 및 용토가 묘생장에 미치는 영향을 조사하였다. 용토의 물리성을 측정한 결과 가비중, 진비중 및 공극율은 퍼얼라이트에서 가장 높게 나타났으며 수분보유량은 코코피트에서 가장 높게 측정되었다. 162, 129, 72, 50구 플러그셀 트레리별로 퍼얼라이트 코코피트 그리고 퍼얼라이트+코코제트(1:1)을 채우고 60일간 육묘하여 묘의 생육을 조사하였다. 묘의 엽수는 125구 코코피트 처리에서 가장 많았으며 엽면적은 50구 코코피트 처리에서 높게 나타났다. 신초와 뿌리의 생체중 및 건물중 그리고 엽록소 함량은 퍼얼라이트+코코피트 처리에서 높게 측정되었다. 플러그셀 크기가 클수록 유모 생장에 효과적이었으며 퍼얼라이트 단용 보다는 코코피트와의 혼용 용토에서 유묘 생육이 가장 양호하였다. This study was conducted to examine the effects of plug tray cell size and growth media on good seedling production of Gerbera hybrida Hort. Seedlings were worm for 60 days in 50, 72, 128, 162 cell trays continuing perlite, cocopeat and perlite+cocopeat(1:1, v/v). Perlite showed higher bulk density than cocopeat and perUte+cocepeat. Total porosity was greater in perlite, cocopeat and perlite+cacopeat in order. Cocopeat showed the highest water balding capacity. Number of leaves were greatest in 128 cell tray containing cocopeat. Leaf area was greatest in 50 cell tray containing cocopeat. Seedling growth was also better in plug tray of bigger cell size. Seedling growth of fresh and dry weight of shoot and root was much better in the growth media of perlite+cocopeat.