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林雄圭,任慶彬 한국식물학회 1977 Journal of Plant Biology Vol.20 No.2
Two sample varieties of Yushin and Jinheung were used for the experiment in low-level wetty paddy field which was arranged with PVC pipe underdrainage of 6 m in distance and 60㎝, 90㎝, and 120㎝ in depth and control, using sprit plot design with three replications. On the yield of brown rice, Yushin was increased by 24.8% in the 90㎝ plot and Jinheung by 16.7% in the 120㎝ plot, respectively, over the control. The ratio of matured grains of these two varieties was increased significantly by the underdrainage and the number of grains per head of Yushin was also increased in drained plots. Otherwise, the underdrainage enhanced the plant height in the early growth of rice plant and further increased the culm height and panicle legnth. It seemed that these results might enhance the light-receiving efficiency in the latter growth of rice plant and bring about the effect of increased yields by the underdrainage.
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임웅규,김해중 한국작물학회 1986 Korean journal of crop science Vol.31 No.2
돌외의 사포닌 성분을 비교하기 위하여 경남 거창지방과 일본 덕도지방에서 수집한 돌외의 잎과 줄기에서 사포닌을 추출 정제하여 HPLC로 분석하고, 인삼의 사포닌 성분과 비교하기 위하여 HPLC의 크로마토그램의 retention time에 의하여 비교하였다. 돌외의 사포닌 성분을 인삼의 사포닌 성분과 달랐으며, 두 지방에서 수집한 돌외의 사포닌 성분은 서로 비슷하였다. 사포닌의 함량은 거창지방에서 수집한 돌외가 일본 덕도지방의 것보다 0.26 % 더 많았다. Saponins of Gynostemma pentaphyllum that collected from Geochang region(Korea) and Dokujima(Japan) were extracted by the method for ginseng saponin. Comparison by retention time in chromatogram(HPLC) of G. pentaphyllum to that of ginseng showed that it is hardly to find out a common saponin between penta-phyllum and ginseng saponins. Saponin content extracted from G. pentaphyllum growing in Geochang region was higher than that growing in Dokujima region.
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임웅규 한국작물학회 1990 한국작물학회지 Vol.35 No.2
한국에서 자생군락이 비교적 큰 3지역에서 채집한 달맞이꽃 종자의 필수 지방산을 Capillary column gas chromatography로 분석하였다. 각 지방산의 조성 함량은 palmitic acid가 6.19-6. 73%, stearic acid가 1.84-l.99%, oleic acid 가 6.73-9.10%, linoleic acid가 74.41-75.53%이었다. 특히 prostaglandin의 전구체인 GLA는 청주가 9.14%, 제천이 9.32%이고, 안동이 8.31%이었다. 따라서 한국의 자생 달맞이꽃은 GLA자원으로 유용한 것으로 생각된다. The essential fatty acid composition of evening primrose seed at 3 natural habitats in Korea was investigated by means of capillary column gas-chromatography. The content of essential fatty acid components was palmitic acid 6.19-6.73%. stearic acid 1.84-1. 99%, oleic acid 6.73-9.10%. linoleic acid 74.41-75.53%. Particularly. gamma-linolenic acid (GLA) which is a precursor of prostaglandin was 9.14% in Cheong-ju. 9.32% in Che-cheon and 8.31% in An-dong. Based on the GLA content evening primroses in Korean natural habitats seem to be very promising and useful for GLA production.
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임웅규 한국식물학회 1974 Journal of Plant Biology Vol.17 No.4
The present investigation dealt with observation and experiments concerning the growth and differentiation of hop plant, using the varieties of Cascade(C), Shinshuwase(Sh), and Hallertau(H). The results were as follows: (1) Life cycle of hop plants. The annual growth period of hop plant was devided chiefly into 3 phases, dormant, vegetative and reproductive. (2) Growth of main stem. The hop vine begun to grow in the middle of May and grow vigorousely in the middle and latter of June, then gradually decline or stops at the middle of July and the early of August. (3) Growth of lateral vines. By the statistical analysis, it is judged that the varieties of H and Sh were more grown than that of C. H and Sh were not significant, but H and Sh from C were significant in 5% level. (4) Fresh weight and water content of hop cone. Hop cone in fresh weight of C variety was higher than those of other two varieties and water content of hop cone was decreased with time elapse in three races together. (5) Growing point. Histological view of hop varities in each was different. C showed form of sweet potato, H showed form of round, and Sh showed form of ellipse. (6) Shape of the leaf. C and H were 3 lobes, but Sh is 3∼5 lobes. Generally, the color is dark green. (7) Hop cones. Hop cones are as follows.
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林雄圭 서울大學校 農科大學 1978 서울대농학연구지 Vol.3 No.1
Three sample varieties of Sh, H and C were used for Experiment which treated with ureafoliar application of 3 levels, using split plot design with 3 replication in hop garden of College of Agriculture in Suweon. In the stem of growth, 1 level was highly significant. In the fresh weight and dry weight of 100 hop cone, 1 level was significant. In the production of hop cone per one tree, 1 level was significant. In chemical component of α-acid, Sh ₁increased 6.74%, H ₁increased 5.24%, and C ₁increased 11.37% as compared with control In conclusion, urea-foliar application of hop is 0.5% solution.
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임웅규 서울大學校 農業生命科學大學 農業開發硏究所 1995 서울대농학연구지 Vol.20 No.2
건조된 백화사설초 1.2kg을 methanol로 3일간 추출한 후 methanol 조추출물을 hexane, chloroform, ethyl acetate, water로 분획하여 짙은 녹색 분말상의 HEF 35.81g, 짙은 황색의 syrup 상의 CHF 1.04g, 황색의 syrup 상의 EAF 0.98g, 연한 갈색 분말의 WAT 4.03g을 얻었다. 한편 백화사설초 1kg을 열수추출하여 얻은 다당체의 양은 7.14g이었다. HEF 분획에는 terpenold, steroid, anthraquinone이 함유되어 있는 것으로 밝혀졌고, CHF에는 terpenoid, steroid가 함유되어 있는 것으로 밝혀졌으며 소량의 alkaloid가 함유되어 있는 것으로 추정되었다. EAF에는 terpenoid, steroid가 검색되었고, 미약한 반응을 보이는 flavonoid가 검색되었다. WAT 분획에서는 saponin이 검색되었다. 백화사설초 전초의 일반 성분은 수분의 경우 9.5%, 회분 13.5%, 조지방 7.5%, 조단백질 2.0%, 조탄수화물 61.5%가 각각 함유되어 있었다. 생리활성분획으로 판단된 EAF를 여러가지 chromatography 법으로 분리, 정제하여 활성분획 EAF9를 얻었으며 이후 ODS로 정제하여 EAF93을 얻었으며, HPLC로 분취하여 활성분획 EAF933, EAF934, EAF935를 얻었다. 각 HPLC에서 얻어진 분획을 화학적 검색법으로 검색한 결과, 항종양작용 관련 성분은 terpenoid, coumarin계 물질이었다. 항균작용 관련 성분은 iridoid계 물질이었으며, 백화사설초 열수추출 다당체는 고등균류와 같은 단백질결합다당체가 아닌 거의 당만으로 이루어진 성분이었다. Dried H. diffusa(1.2kg) was extracted with methanol for three days, and then crude methanol extract(CME) was sequentially partitioned with n-hexane, chloroform, ethyl acetate and water to give 35.8lg of hexane extract fraction(HEF) of greenish powder, 1.04g of chloroform fraction(CHF) of dark yellow syrupy substance, 0.98 of ethyl acetate fraction(EAF) of yellow syrupy substance, and 4.03g of water extract fraction(WAT) of weak brownish powder. Hot water extract(CWE) from 1kg of H. diffusa(1kg) was polysaccharide and its mass was 7.14kg. The active fractions were chemically analyzed and purified through various chromatography to obtain purified substances EAF933, EAF934, and EAF935. Color change test and bioassay were performed on the fractions. The result suggested that antitumor components were terpenoid and coumarin while antimicrobial components were iridoid. Chemial analysis of CWE showed that the polysaccharide was consisted of sugars only unlike protein-bound polysaccharides from higher fungi(Data of bioassay are not shown).
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