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장티푸스환자의 혈청내 Salmonella typhi에 대한 IgG subclass항체의 분포
김영중,황응수,강재승,차창룡,장우현,김윤원,조민기,민창홍,Kim, Young-Jung,Hwang, Eung-Soo,Kang, Jae-Seung,Cha, Chang-Yong,Chang, Woo-Hyun,Kim, Yoon-Won,Cho, Min-Ki,Min, Chang-Hong 대한미생물학회 1986 大韓微生物學會誌 Vol.21 No.4
To diagnose the typhoid fever rapidly and accurately in clinically suspected patients, the levels of IgG subclass antibody were measured by enzyme-linked immunosorbent assay(ELISA). With symptom, blood culture and agglutination test, tested persons were categorized into 6 groups as typhoid fever, FUO, paratyphi A or B, other bacterial infctions, cancers, and control. ELISA was performed on the polyvinyl chloride plates coated with killed whole cell($10^8\;cell/ml$) of S. typhi 0901W by poly-L-lysine applied as binding substance (and polyvinyl chloride as solid phase). The distribution of the level of IgG subclass antibodies in each group was analyzed and compared with other groups. The results obtained were summarized as follow: 1. The optimal dilution of the sera from patients with typhoid fever was 1:160, and those of the sheep anti-human IgG subclass and the peroxidase conjugated rabbit anti-sheep IgG were 1:4000 and 1:5000, respectively. 2. The absorbance levels of IgG subclass in the sera of typhoid fever patients were as follows; a) IgG1 value is $0.439{\pm}0.110$ b) IgG2 value is $0.416{\pm}0.165$ c) IgG3 value is $0.449{\pm}0.145$ d) IgG4 value is $0.525{\pm}0.154$ IgG subclass levels in the sera of typhoid patients were much higher than in control group and patient with paratyphi A or B as well as other infectious diseases. The sensitivity and the specificity in differential diagnosis of typhoid fever and other febrile diseases were 92% and 79% in the assay of IgG1 respectively, whereas those in the assay of IgG2 were 97% and 72%, respectively (above absorbance 0.3). 3. The absorbance levels of IgG subclass in the serial sera of typhiod fever patients tend to decrease to the level of absorbance 0.3 in 10 months from the onset of illness. 4. The order of absorbance levels of IgG subclass in the serum of each group were typhoid fever, paratyphi A or B, other infectious diseases, control and cancer. 5. For the serodiagnosis of typhoid fever against other febrile diseases, the sensitivity and the specificity in the assay of IgG2 activity were 76% and 93% in absorbance 0.4, respectively. 6. In the distribution of the level of each IgG subclass in the sera of FUO patients which were suspected of typhoid fever, the positive rate was ranged from 36% to 82%. This suggest that more than 50% of FUO patients are caused by S. typhi.
조리 및 식음료 종사원이 인지하는 직무 자율성이 잡크래프팅과 직무만족도에 미치는 영향
김영중,정윤조 한국외식경영학회 2019 외식경영연구 Vol.22 No.3
The purposes of this study were to understand the influence of job autonomy on job crafting and job satisfaction. Based on a total 278 samples obtained form empirical research, this study reviewed the reliability and fitness of the research model and verified a total of 7 hypotheses with the Amos program. The hypothesized relationships in the model were tested simultaneously by using a structural equation model(SEM). The proposed model provided an adequate fit for the data =482.532(df=201), p<.001, CMIN/df=2.401, GFI=.864, AGFI=.909, TLI=.936, CFI .944, RMSEA=.071. The model’s fit, as indicated by these indices, was deemed satisfactory; thus, it provided a good basis for testing the hypothesized paths. The SEM showed the relational job autonomy had a positive job crafting and job satisfaction. Also, job crafting had a positive significant influence on job satisfaction. Limitations and future research directions are also discussed 본 연구는 조리 및 식음료 종사원이 인지하는 직무 자율성이 잡크래프팅과 직무만족도에 어떠한 영향을 미치는지에 대해 실증분석해보고자 하였다. 실증연구를 수행하기 위해 확보된 278개의 표본을 바탕으로 연구 모형의 신뢰성, 적합성 등을 검토하였고, 구조방정식을 사용하여 총 7개의 가설을 검증하였으며, 모형의 적합도는 =482.532(df=201), p<.001, CMIN/df=2.401, GFI=.864, NFI=.909, TLI=.936, CFI=.944, RMSEA=.071 등으로 조사되었다. 연구결과, 직무 자율성은 과업 경계변화, 인지 경계변화, 관계 경계변화에 유의한 정(+)의 영향을 미치는 것으로 나타났으나 직무만족도에는 유의한 영향을 주지 않는 것으로 조사되었다. 한편 잡크래프팅의 인지 경계변화, 관계 경계변화는 직무만족도에 유의한 정(+)의 영향을 주는 것으로 나타났다. 이러한 결과를 통해 조리 및 식음료 종사원의 직무 자율성 보장을 통해 잡크래프팅을 거쳐 직무만족도를 향상시키는 것이 중요할 것이다.