Korean Red ginseng prevents endothelial senescence by downregulating the HO-1/NF-κB/miRNA-155-5p/eNOS pathway

Tae-Hoon Kim,Ji-Yoon Kim,Jieun Bae,Young-Mi Kim,Moo-Ho Won,Kwon-Soo Ha,Young-Guen Kwon,Young-Myeong Kim 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.2

Background: Korean Red ginseng extract (KRGE) has beneficial effects on the cardiovascular system by improving endothelial cell function. However, its pharmacological effect on endothelial cell senescence has not been clearly elucidated. Therefore, we examined the effect and molecular mechanism of KRGE on the senescence of human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were grown in normal or KRGE-supplemented medium. Furthermore, they were transfected with heme oxygenase-1 (HO-1) gene or treated with its inhibitor, a NF-κB inhibitor, and a miR-155-5p mimic or inhibitor. Senescence-associated characteristics of endothelial cells were determined by biochemical and immunohistochemical analyses. Results: Treatment of HUVECs with KRGE resulted in delayed onset and progression of senescence-associated characteristics, such as increased lysosomal acidic β-galactosidase and decreased telomerase activity, angiogenic dysfunction, and abnormal cell morphology. KRGE preserved the levels of anti-senescent factors, such as eNOS-derived NO, MnSOD, and cyclins D and A: however, it decreased the levels of senescence-promoting factors, such as ROS, activated NF-κB, endothelial cell inflammation, and p21 expression. The beneficial effects of KRGE were due to the induction of HO-1 and the inhibition of NF-κB-dependent biogenesis of miR-155-5p that led to the downregulation of eNOS. Moreover, treatment with inhibitors of HO-1, NF-κB, and miR-155-5p abolished the anti-senescence effects of KRGE. Conclusion: KRGE delayed or prevented HUVEC senescence through a signaling cascade involving the induction of HO-1, the inhibition of NF-κB-dependent miR-155-5p biogenesis, and the maintenance of the eNOS/NO axis activity, suggesting that it may protect against vascular diseases associated with endothelial senescence.

청심연자탕(淸心蓮子湯)으로 호전된 뇌내출혈에 고혈압이 동반된 태음인 환자 치험 1례

오정민,엄태민,최고은,허종원,유호룡,설인찬,김윤식,Oh, Jeong-Min,Eom, Tae-Min,Choi, Ko-Eun,Heo, Jong-Won,Yoo, Ho-Ryong,Seol, In-Chan,Kim, Yoon-Sik 대한중풍순환신경학회 2014 대한중풍.순환신경학회지 Vol.15 No.1

■ Objectives The purpose of this case study is to report that a hypertension with intracerebral hemorrhage of tae-eumin was treated with 'Cheongsim Yeunja-tang' and then the symptoms were improved and blood pressure was decreased. ■ Methods Although the patient who had hypertension with intracerebral hemorrhage took antihypertension drugs, intermittent increase of blood pressure was shown. We diagnosed him as Tae-eumin and treated with Cheongsim Yeunja-tang. We daily checked blood pressure and evaluated the symptoms. ■ Results After the treatment with Cheongsim Yeunja-tang, blood pressure was decreased and symptoms were improved. ■ Conclusion The result shows Cheongsim Yeunja-tang has antihypertensive effect and improve hypertension symptoms of Tae-eumin patient with intracerebral hemorrhage.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

효모로부터 젖산 탈수소 효소(LDH)의 분리

윤태호,김인호 충남대학교 산업기술연구소 2000 산업기술연구논문집 Vol.15 No.1

Lactate Dehydrogenase from Saccharomyces cerevisiae was separated. Yeast cultured in glucose media were obtained by centrifuge from broth and cell pellet was stored in freezer at -20℃. The cell in the freezed yeast pellet suspended in 0.01M phosphate buffer(pH=7.0), was destrupted by French press. After cell destruption, supernatant prepared by centrifuge to remove cell debris was fractionally crystallized with (NH4)2SO4 to gain enzyme. The lactate dehydrogenase had maximum yield at 40-60% saturation of (NH4)2SO4, and precipitates were stored again in freezer at -20℃. To obtain more purified enzyme, we loaded protein sample on chromatography column packed with Sephadex G-200 or DEAE Bio-Gel A. The activity of lactate dehydrogenase separated by Sephadex G-200 column chromatography was 84U/mg, and 9.47 times higher than that of crude solution. Futhermore, the activity of lactate dehydrogenase separated by DEAE Bio-Gel A column chromatography was 4118U/mg, this value was 464 times higher than that of crude solution.

집중질량 모델에 의한 건물의 동적해석

윤태호 國立 昌原大學校 産業技術硏究所 1999 産技硏論文集 Vol.13 No.-

The lumped parameter mass(LPM) model approach is investigated. LPM model is widely used in nonlinear dynamic analysis of tall buildings under lateral loads. The parameters of LPM model are obtained through static pushover anaysis of the three dimensional frame model using CANNY and the story force-drift relationship is assumed as tri-linear force-displacement relationship to represent the behavior of the lumped model. El-Centro NS components are used for earthquake excitation and the intensity is controlled by changing the maximum ground acceleration : PGA=0.034g for elastic response and PGA=0.3g for inelastic response. The results from the three dimensional nonlinear dynamic analysis of the frame model are compared with those of lumped model analysis. In both cases the analysis of the three dimensional and LPM model show similar results and the results from the lumped model turn out to be quite sensitive to the determination of the parameters.

K₂O·CaO·P₂O_5조성의 구조와 용출특성에 관한 연구

윤영진,윤태민,이영수,강원호 단국대학교 2001 산업기술연구 Vol.2 No.-

Various composition of K_2O·CaO·P_2O_5 glasses were studied by melting process to identify glass forming region. Thermal properties were observed by DSC and TG-DTA, structural properties were investigated by FT-IR and Raman Spectroscopy. Dissolution mechanism was observed by pH meter and Optical Microscopy. The glass transition temperature(Tg) and softening temperature(Ts) were decreased with decreasing CaO content. Tgand Ts was increased from 318℃ to 536℃, Ts was from 350℃ to 563℃ respectively with increasing CaO content, α was decreased from 201.5×10 exp (7) to 90.1×10 exp (7), as CaO content was increased, dissolution properties was decreased, pH has been changed acid as the times.

저압의 유기금속 화학증착법에 의해 성장된 Ta₂O5 박막의 계면물성

윤재홍,박태곤,김명호,이찬규 國立 昌原大學校 産業技術硏究所 1994 産技硏論文集 Vol.8 No.-

High quality Ta₂O? dielectric films were deposited by low-pressure metalorganic chemical vapor deposition (LPMO-CVD) from Ta(OC₂H?)? and oxygen. The microstructure and Ta₂O?/SiO₂/Si interfacial properties were investigated by XRD, SIMS, SNMS, RBS, SEM and TEM, XRD analysis showed that the structure of as-deposited thin films was amorphous, but it was crystallized to orthorhombic β-Ta₂O? by after-annealing at temperatures higher than 700˚C. The SiO₂layer at TiO₂/Si interface is strongly dependent on after-annealing conditions and dominates the electrical properties of Ta₂O? thin films on Si.

변단면 보-기둥 용접접합부의 전단응력

윤태호,허택녕 國立 昌原大學校 產業技術硏究院 2004 産技硏論文集 Vol.18 No.-

This study suggests that in order to reduce off-face shear stress of beam flange a tapered beam can be employed, rather than attaching triangle haunch to the beam, to improve the effective depth of beam itself in the vicinity of connection, as analyzed by a limited factors analysis about beam-column welded moment connection the fact that the development of severe shear stress in the beam flange leads to be causes of brittle fracture on welded region, and suggests that the welded moment connection using papered beam can help in improve seismic performance on the beam-column welded moment connection, as analyzed transfer mechanism of shear and bending stress by a limited factors analysis about tapered beam and column connection.

스트레스 대처방식이 조혈모세포 이식 환자의 우울과 불안에 미치는 영향

윤수정,이철,임성후,채정호,전태연,김광수 한국정신신체의학회 2002 정신신체의학 Vol.10 No.1

저먼 세퍼드에서 발생한 내측 갈고리돌기 유합부전을 컴퓨터 단층촬영술을 이용하여 진단한 증례

황윤태,안지영,신희승,최호정,이영원 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2009 動物醫科學硏究誌 Vol.16 No.1

A 4-year-old male German shepherd dog with reluctance to exercise, swelling of bilateral elbow joint and general depression was referred to the Veterinary Medical Teaching Hospital of Chungnam National University. Swelling, alopecia and lichenification of bilateral elbow joint were observed on physical examination. Osteophytes, sclerosis and joint mouse of bilateral elbow joint were found on radiography. Deformation of medical coronoid process and soft tissue density swelling at caudal part of bilateral elbow joint were also observed on radiography. Computed tomography revealed fragmented medial coronoid process and osteophytes of bilateral elbow joint. Therefore this case was diagnosed as fragmented medical coronois process and degenerative joint disease.