Clinicopathological and molecular analysis of multinodular and vacuolating neuronal tumors of the cerebrum

Choi, Euno,Kim, Seonk-Ik,Won, Jae-Kyung,Chung, Chun Kee,Kim, Seung Ki,Choi, Seung-Hong,Choi, Seongmin,Han, Bome,Ahn, Bokyung,Im, Sun-Wha,Park, Sung-Hye Elsevier 2019 Human pathology Vol.86 No.-

<P><B>Summary</B></P> <P>Multinodular and vacuolating neuronal tumor (MVNT) of the cerebrum is a recently recognized rare neuronal tumor, and its pathogenesis is unclear. We analyzed 7 cases of histologically typical MVNT: 6 were adults (mean age, 43.0 years [range, 23-56 years]) and 1 was a child (age, 10 years). The most common symptoms were seizures (n = 4) and headache (n = 2). The tumors were supratentorial (temporal, 5; frontal lobes, 2) in origin as reported. Vacuolated tumor cells were robustly positive for α-INA and Olig2 and at least partly positive for synaptophysin and MAP2, but negative for Neu-N, nestin and CD34. GFAP and vimentin were expressed in reactive astrocytes but not in tumor cells. Negative results were obtained for p53, IDH-1, BRAF<SUP>V600E</SUP>, H3 K27M, EGFR, Lin28A, and L1CAM. ATRX, BRG1, INI-1, and TMHH were retained. The Ki-67 labeling index was very low (<1%), and pHH3 revealed no mitotic figure. Ultrastructural features of tumor cells were comparable with those of immature neuronal cells, with several intracytoplasmic myelin-like autophagosomes and pericellular vacuolization. No <I>IDH1</I>/<I>IDH2</I> and <I>BRAF</I> <SUP> <I>V600E</I> </SUP> mutations were found upon direct sequencing. Whole-exome sequencing revealed <I>FGFR2</I>-<I>ZMYND11</I> gene fusion in 1 case. After gross total resection, all patients were alive without seizures. There was no tumor recurrence during an average period of 68 months (range, 23-101 months). The analysis of 7 typical cases of MVNT suggested that these lesions may be clonal tumors because <I>FGFR2</I>-<I>ZMYND11</I> fusion was found (1 case).</P> <P><B>Highlights</B></P> <P> <UL> <LI> “MVNT” of the cerebrum is a recently recognized rare epileptogenic neuronal tumor. </LI> <LI> Whole-exome sequencing revealed <I>FGFR2</I>-<I>ZMYND11</I> gene fusion in one of our MVNT cases. </LI> <LI> The tumor cell is robustly positive for α-INA and Olig2, but negative for Neu-N. </LI> <LI> Ultrastructurally, tumor cells have autophagolysosomes with pericellular vacuolization. </LI> <LI> There was no tumor recurrence during an average period of 68 months. </LI> </UL> </P>

Effects of Streptozotocin-Induced Type 1 Diabetes on Cell Proliferation and Neuronal Differentiation in the Dentate Gyrus : Correlation with Memory Impairment

Jung Hoon Choi,In Koo Hwang,Sun Shin Yi,Ki-Yeon Yoo,Choong Hyun Lee,Hyung-Cheul Shin,Yeo Sung Yoon,Moo-Ho Won 대한해부학회 2009 Anatomy & Cell Biology Vol.42 No.1

We examined the effects of steptozotocin (STZ)-induced type 1 diabetes on cell proliferation and neuroblasts in the subgranular zone of the hippocampal dentate gyrus (SZDG) of male Wistar rats. Change in memory function was also investigated using the passive avoidance test. In the SZDG, Ki67 (a marker for cell proliferation) positive nuclei were significantly decreased at 2 and 3 weeks and slightly decreased at 4 weeks after STZ treatment. Doublecortin (DCX, a marker for neuronal differentiation)-immunoreactive (+) neuroblasts with tertiary dendrites were significantly decreased in the STZ-treated group compared to those in the vehicle-treated group. However, DCX+ neuroblasts without tertiary dendrites were abundant at 4 weeks after STZ treatment. In addition, retention latency time in STZ-treated group was similar to that of vehicle-treated group at 2 and 3 weeks after STZ treatment. However, the retention latency time was significantly decreased at 4 weeks after STZ treatment. These results suggest that STZ significantly reduced cell proliferation and neuroblasts at 2~3 weeks after STZ treatment, but not at 4 weeks after STZ treatment although memory impairment was detected at 4 weeks after STZ treatment. The gradual reduction of DCX+ neuroblasts with tertiary dendrites may be associated with the impairment of hippocampus-related memory function.

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.

하악골 성장 지표로서 경추골 성숙도의 유용성

최봉선,최남기,김선미,양규호,정성수 大韓小兒齒科學會 2007 大韓小兒齒科學會誌 Vol.34 No.4

This study was to investigate if cervical vertebrae maturation stages are as useful as hand-wrist maturation stages in evaluating the mandibular growth. The subject consisted of 292 girls aged from 8 to 16 years with normal occlusion. They were classified according to diagnosis by using studycast. lateral cephalogram, and handwrist X-ray film. The results were as follow: 1. Cervical vertebrae and hand-wrist maturation stages increased with age. 2. All mandibular measurements (Ar-Go, Go-Me, N-Go, S-Gn, N-Me) increased linearly with cervical vertebrae maturation stages. 3. Ar-Go, Go-Me, N-Go, S-Gn increased linearly with hand-wrist maturation stages. 4. Ar-Go, Go-Me, N-Go, S-Gn increased relatively rapidly between cervical vertebrae maturation stages 3 and 4. Go-Me and S-Gn increased relatively rapidly between hand-wrist maturation stages 6 and 7. 5. Ar-Go, Go-Me, N-Go, S-Gn, N-Me had high correlations with cervical vertebrae maturation stages as well as hand-wrist maturation stages. These results suggest that cervical vertebrae maturation stages are reliable on evaluating the mandibular growth. 하악골 성장을 평가하는 데 경추골 성숙도가 수완부골 성숙도만큼 유용한가 규명하고자 본 연구를 시행하였다. 정상교합을 가진 만 8세∼만 16세 여성 292명을 연구대상으로 하여 진단석고모형,측모두부규격방사선사진,그리고 수완부방사선사진을 이용하여 경추 및 수완부의 골성숙도를 비교하여 다음과 같은 결과를 얻었다. 1. 연령 증가에 따라 경추 및 수완부의 골성숙도가 일정하게 증가하였다. 2. 경추골 성숙도가 증가할수록 Ar-Go,Go-Me,N-Go,S-Gn,N-Me이 일정하게 증가하였다. 3. 수완부골 성숙도가 증가할수록 Ar-Go,Go-Me,N-Go,S-Gn이 일정하게 증가 하였다. 4. 경추골 성숙도 3단계와 4단계 사이에서 Ar-Go,Go-Me,N-Go,S-Gn이 크게 증가하였고,수완부골 성숙도 6단계와 7단계 사이에서 Go-Me,S-Gn이 크게 증가 하였다. 5. Ar-Go,Go-Me,N→Go,S一Gn,N-Me은 경추 및 수완부 골성숙도와 각각 유의 한 상관성을 보였다. 이상의 결과는 하악골 성장을 평가하는 데에 경추골 성숙도가 수완부골 성숙도처럼 유용하게 사용될 수 있음을 시사한다.

경상돌기연장증 2례

최종욱,권기환,전병선,김정준,최건 한국의학사 1998 診斷과治療 Vol.18 No.9

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

殘留應力을 考慮한 鎔接材의 疲勞龜裂 進展擧動에 關한 硏究

최병기,이을호,국중민,기준호,임선빈,정장만 朝鮮大學校 機械技術硏究所 2000 機械技術硏究 Vol.3 No.1

The effect of welding residual stress on the initiation and propagation of fatigue crack is investigated so as to study the fatigue behavior in welding residual stress field. Especially, the relation between welding residual stress and fatigue crack opening behavior is investigated and the redistribution of residual stress as a fatigue crack propagates is examined. Following results are obtained. 1) In case of the load range is constant, as the stress ratio is changing to 0.1, 0.33, 0.5 the propagation life is constant but the growth life decreases. And than, when maximum load or minimum load is constant, as the stress ratio increases the growth life and propagation life increase. 2) The fatigue crack propagations rate da/dN appears to accelerate when the stress intensity factor range ΔK is less than 15MPa-m^1/2. 3) The residual stress that is appeared by welding decreases after cutting. 4) Fatigue crack propagation ratio is affected by tensional and compressible residual stress at the initial time, but it's similar to the original in the more than 20mm of crack length.

레이져 마킹을 위한 갈바노미터 구동에 관한 연구

최치영,홍선기,강태삼,김수길 호서대학교 반도체제조장비국산화연구센터 2001 반도체장비학술심포지움 Vol.2001 No.-

본 논문에서는 레이져 마킹용 갈바노미터의 구동을 위하여 마킹에 필요한 벡터 폰트의 개발환경 구축하고, 만들어진벡터 폰트를 PC와 구동 장치간의 통신용 인터페이스를 제작하여 갈바노미터(galvanometer)에 송신, 출려할 수 있도록 하였다.벡터 폰트의 곡선 처리는 베지어(Bezier) 함수를 사용한 외곽선 폰트(outline pont)를 사용하였다.폰트 에디터를 이용하여 문자를 작성한 후 문자의 외곽선 정보를 인터페이스 카드에 보내고, 이 데이터를 D/A변환기를 통해 갈바노미터 구동장치를 구동하고, 레이져를 갈바노미터 반사경에 조사하여 폰트를 출려할 수 있도록 하였다.인터페이스 카드는 16비트 D/A변환기를 이용하여 PC의 ISA 버스를 이용하였다.실험을 통해 개발된 장치의 구동이 올바르게 동작함을 확인하였다.

酵母細胞의 인산대사 및 유기물 합성에 미치는 무기인산 화합물의 영향

崔榮吉,이기성,李鍾三,趙善姬 漢陽大學校 環境科學硏究所 1983 環境科學論文集 Vol.4 No.-

효모의 개체군 생장에 미치는 인산 화합물(??) 및 ??, ?? 양이온의 영향을 측정한 결과, ??는 Knopp씨 배지수준의 1배, 10배, 50배 처리의 전구간에 걸쳐서 개체군 생장에 유의한 촉진효과를 볼 수 없었다. ?? 처리구에서는, ?? 의 1배 처리구에서 대조구에 비하여 뚜렷한 생장촉진효과를 볼 수 있었으나, 10배, 50배의 처리구에서는 오히려 생장이 감소함을 나타내었다. 또한 ?? 처리구는 전 구간에 걸쳐서 대조구에 비하여 뚜렷한 억제가 관찰되었다. 한편, 효모의 인산화합물 합성에 미치는 영향을 측정하여 본 결과, ??의 경우는 산 가용성 Total-P 가 전 구간에 걸쳐 배양 처리후 6일째에, 대조구에 비하여 증가하였으며, ??처리구는 전처리구에서 산가용성 Total-P가 감소하였으나 ortho-P만은 증가하였음을 보였다. ??의 처리구에서도 Total-P는 역시 대조구에 비하여 감소하고 ortho-P는 대조구와 비슷한 경향을 보였다. poly-P "A", "B", "C", type 의 소장 관계를 측정한 결과, poly-p-A 와 B는 인산농도 및 양이온의 농도가 높을수록 많은 생성량을 나타내었으며, 특히 ?? ×50 처리구에서 poly-p-"B" 의 최대 합성량을 볼 수 있었다. 또한 여러 배양조건하에서도, 여러가지 poly-P 전환과정은 모든 시험구에서 동일한 것으로 나타났다. 그리고 특히 ?? 이온에 의해 영향받는 poly-P의 유기물합성에 이용되는 전환경로와 poly-P 상호 전환경로는 다음의 관계가 성립하는 것으로 추론된다. ?? 한편, 효모세포의 단백질, 핵산, 인지질, 탄수화물 합성에 미치는, 이들 인산 화합물의 양적 동태를 고찰한 결과, 핵산의 경우, ?? 처리구는 모든 구간에서 핵산 합성에 유의한 효과를 찾을 수 없었으며, ??처리구는 1배농도의 처리구에서는, 핵산의 합성증가를, 그외의 처리구에서는 합성량이 낮았다. ?? 처리구에서는 전체적으로 현저한 유기물의 합성억제를 나타내었다. 단백질 합성의 경우는 ??×1처리구를 제외한 모든 처리구에서 대조구와 비교하여 비슷한 경향을 나타내거나 억제효과를 보여주었을 뿐,촉진효과를 관찰할 수 없었다. 인지질 합성시 ??는 촉진효과를 나타내었으나 ?? 와 ?? 는 유의한 효과를 볼 수 없었다. 효모의 탄수화물 합성에 있어 ?? 는 대체로 대조구의 합성량과 유사하며, ?? 의 경우와 ??의 경우는 전 처리구간에서 대조구에 비하여 배양초기에 억제효과를 나타내었으나, 배양후기에는 오히려 증가되었다. 한편 poly-P와 유기물 합성과의 상관관계에서, Polysaccharide,lipid-p, DNA 합성에는 Poly-P-B가, nucleotidic-labile-P 생성에는 poly-P-C 가 상호 연관된 것으로 추정되었다. Examined the effect on the growth of yeast population by treating medium with phosphate compounds such as ??, ?? and ?? ion as the the concentration of Knopp's level, tenfold concentration and of fiftyfold, respectively, it could be recognized as the following facts; in case of ??, it showed no significant effect on the growth of yeast population in all treatmemt. And in case of ?? treatment replaced K? of KH₂PO₄in knopp's medium it showed the stimulating effect in the culture as comparing with those of control, but on the contrary, it was revealed the inhibition effect in the culture of tenfold and fifty fold treatment. However, the addition of ?? compounds showed the clear evidence of inhibition effect in population growth. Measuring the effect on the synthesis of phosphate compounds of yeast cell by treating the above chemicals, the amount of acid soluble-total phosphate was increased in all ?? teatment on 6th day after treatment. On the contraty, in case of ?? treatment, the amount of acid soluble-total phosphate was decreased in all treatment comparing with that of control, but the amount of ortho-phosphste was increased in all cultures. These similar tendencies were also found in the cultures of As?? compound treatment. The amount of acid soluble-total phosphate was decresed comparing with that of control and ortho-phosphate was as of control level. The accumulation amount of each type of polyphosphate(A,B and C) seemed to have a relationship between each other. It seemed that the amounts of polyphosphate A and B in yeast cell are increased in accordance with high cation concentration and phosphate concentration in culture medium. Especially, the culture in fiftyfold concentration of ?? ion synthesized the highest amount of polyphosphate B. However, the trun-over patterns of each polyphosphate under various conditions showed similar tendencies with each other. But the patterns utilizing in synthesis of cellular organic matter are belived to be affected by ?? ion as the direction in the following figure. ?? The synthesis of cellular organic matter, such as protein, nucleic acid, phospholipid , and polysaccharide was also belived to have a relation to cellular nutritional conditions. Any treatment of ?? showed no significant effect in synthesis of nucleic acid . The treatment of ?? compounds was resulted in decreased of nucleic acid generally, but increase in the culture replaced ?? of KH₂PO₄of Knopp's medium by ??. However, all the treatment of ??) compounds to medium showed strong inbibition effect in synthesis of nucleic acid. The synthesis of cellular protein is promoted by treating ?? compounds as Knopp's level. but in case of tenfold and fiftyfold concentration, the amount of protein is equivalent to or below to that of control. Referring to synthesis of phospholipid, ?? treatment was effective, but ?? and ?? compounds showed inbibition effect. The amount of carbohydrate in yeast cell treated by ?? is similar to those of control, however, in the cell treated by ?? or ?? compounds, the amount was increased just after decreasing in early stages of incubation. The relationship between each polyphosphate and synthesis of cellular organic matter could be interpretated that polyphosphate B is utilized mainly in synthesis of polysaccharide, phospholipid and DNA, and that polyphosphate C is also related in formation of nucleotidic labile phosphate.

개에서 발생한 양파 섭취에 의한 하인즈 소체 용혈성 빈혈 증례

이미선,윤기영,서경원,최호정,정성목,송근호,조종기,박성준 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2012 動物醫科學硏究誌 Vol.19 No.1

A 10 month-old Yorkshire Terrier was referred to the Veterinary Medical Teaching Hospital of Chungnam National University with the history of lethargy, bile pigment in urine and anorexia. Blood smear showed Heinz body, polychromatocytosis, schistocytosis, anisocytosis, which were the results from the oxidative injury. Hematology demonstrated a moderate anemia(PCV 22.4%, reference rage 35-55%), which was regeneration based on the degree of RPI (4.03, reference rage > 1). Serum biochemistry demonstrated mild elevation in total bilrubin, and mild decrease in total protein and albumin concentration. A diagnosis of Heinz body hemolytic anemia secondary to onion ingestion was made and treated with fluid therapy, oxygen and antioxidant drugs. The dog recovered without any complication.