피칸(Carya illinoensis C. KocH)으로부터 지질대사물질의 분리 및 동정

송명종,한민우,양혜정,이대영,노영덕,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

피칸(Carya illinoensis C. Koch) 열매를 80% MeOH로 추출하고, 추출물을 극성에 따라 n-hexane, CHCl_(3), EtOAc, n-BuOH 및 물로 분배, 추출하였다. 이 중 EtOAc분획으로부터 silica gel column chromatography를 반복하여 4종의 지질화합물을 분리하였다. 분리된 화합물 화학구조는, NMR 및 MS 등의 스펙트럼을 해석하여 oleic acid methyl ester, linoleic acid methyl ester, β-sitosterol, daucosterol(3-O-β-D-glucopyaranosyl β-sitosterol)으로 동정하였다. Carya illinoensis C. Koch was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with n-hexane, CHCl_(3), EtOAc, n-BuOH and H_(2)O, successively. From the EtOAc fraction, four compounds were isolated through the repeated silica gel column chromatographies. From the results of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as oleic aicd methyl ester(1), linoleic aicd methyl ester(2), β-sitosterol(3), and daucosterol(4). They were the first to be isolated from C. illinoensis.

국내 다기관에서 조사한 지역사회획득 메티실린내성 황색포도알균의 빈도와 임상적 특성

송진수,최평균,송경호,조재현,김성한,방지환,이창섭,박경화,박경운,신수,최희정,김의석,김동민,이미숙,박완범,김남중,오명돈,김의종,김홍빈,최강원 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

목적 : 최근 전세계적으로 지역사회획득 메티실린내성 황색포도알균(community-associated methicillin-resistant Staphylococcus aureus, CA-MRSA)의 보고가 증가하고 있다. 하지만, 우리나라에서는 CA-MRSA 감염증에 대한 증례보고만 있을 뿐 아직까지 체계적인 연구결과가 없는 실정이다. 저자들은 국내에서 CA-MRSA의 빈도, 감염증의 임상적 양상, 분리된 균주의 항균제내성 양상을 조사하였다. 재료 및 방법 : 2005년 1월부터 2005년 6월까지 7개 병원에서 MRSA가 분리된 환자의 명단을 파악한 후 의무기록지와 건강보험심사평가원의 자료를 검토하였다. 외래나 응급실에서 혹은 입원 후 72시간 이내에 균주가 분리되고 MRSA 획득과 관련된 위험인자가 없는 경우 CA-MRSA로 정의하였으며, 분리된 균주의 임상적 의미에 따라 원인병원체(pathogen), 집락화(colonizer), 미결정(undetermined)으로 분류하였다. Penicillin과 oxacillin을 제외하고 3개 이상의 다른 계열 항균제에 내성이면 다제내성으로 정의하였다. 결과 : 연구기간동안 총 3,251주의 황색포도알균이 분리되었으며, 이 중 MRSA는 1,900주(58.4%)였다. MRSA 가운데 CA-MRSA는 114주(6.0%) 였으며, 이들이 분리된 부위는 귀(62주), 비뇨기계(14주), 피부 및 연부조직(11주), 호흡기계(10주), 혈액(3주) 등이었다. CA-MRSA 균주 가운데 집락균은 22주, 원인병원체는 22주였으며, 나머지 균주에 대해서는 그 임상적 의미를 결정할 수 없었다. 항균제 감수성 검사를 시행한 73균주 중 47주(64.4%)는 다제내성이었다. CA-MRSA 감염증 22예 중 피부 및 연부조직 감염(9예)과 중이염/외이도염(9예)이 가장 흔하였다. 침습적 감염증(invasive infection)은 4명(원발성 균혈증 3예, 감염성 관절염 1예)에서 확인되었지만, CA-MRSA 감염증으로 사망한 환자는 없었다. 결론 : 병원내 감염증에서는 MRSA가 심각한 문제이지만, 아직까지 지역사회 감염증에서 CA-MRSA는 흔하지 않았다. Background : Methicillin-resistant Staphylococcus aureus (MRSA) infection has emerged in patients who do not have the established risk factors. In Korea, little is known about the epidemiology and clinical features of community-associated MRSA (CA-MRSA). Material and Methods : Clinical microbiology laboratory databases of 7 hospitals were reviewed to identify the patients from whom MRSA was isolated during the period of January to July 2005. Only one isolate per patient was enrolled. In order to identify the risk factors of MRSA acquisition, the medical records and the Health Insurance Review Agency databases were reviewed. CA-MRSA was defined as MRSA isolated from patient without established risk factors. We analyzed patient demographics, underlying medical conditions, characteristics of infection, and antimicrobial susceptibility profiles. Results : Of total 3,251 S. aureus isolates, 1900 (58.4%) were MRSAs. Of the MRSA isolates, 114 (6.0%) were CA-MRSA. Of 114 CA-MRSA isolates, 22 (19.3%) were colonizers, 22 (19.3%) were pathogens, and the clinical significance of remaining 70 (61.4%) could not be determined. Median age of the 22 patients with CA-MRSA disease was 47 years. Nine patients had skin and soft tissue infections, 9 ear infections, 3 bacteremia, 1 septic arthritis. Seven patients had underlying medical disease. None died of the CA-MRSA infections. Of the 73 isolates of CA-MRSA, 47 (64.4%) were resistant to more than 3 classes of antibiotics besides β-lactams. Conclusion : Although MRSA is highly prevalent among hospital-associated S. aureus infection, CA-MRSA infections are not common.

쑥갓(Chrysanthemum coronarium L.)으로부터 배당체의 분리

송명종,김세영,송효남,백남인 경희대학교 생명자원과학연구원 2004 硏究論文集 Vol.23 No.-

쑥갓의 지상부를 MeOH로 추출하고, 추출물을 EtOAc, n-BuOH 및 물로 분배, 추출하였다. n-BuOH분획으로부터 silica gel 및 ODS column chromatography를 반복하여 배당체를 분리하였다. 화합물의 화학구조는, MNR 및 MS 등의 스펙트럼을 해석하여 1-o-β-D-glucopyranosyl-3-hexenol으로 동정하였다. The aerial parts of Garland (Chrysanthemum coronarium L.) were extracted in MeOH and solvent fractionated with EtOAc, n-BuOH and water, successively. n-BuOH fraction gave a glycoside compound through application of silica gel column chromatographies including ODS. The chemical structure of the glycoside was determined by the interpretation of several spectral data, including NMR and MS as O-β-D-glucopyranosyl-3-hexenol (1).

中學校 科學科의 實驗實習 敎材의 開1發硏究Ⅰ

郭宗欽,朴炳彬,李元求,宋寅命,崔斗文 공주대학교 사범대학 과학교육연구소 1981 과학교육연구 Vol.13 No.1

The present condition of science education has been investigated b means of questionaires, interviews and referring to literature, and the points at issue gave been extracted on the basis of them, and in consideration of the problems various materials for experiment and practice have been developed to be used for the effective inquiry learning. The present condition and problems of science education in junior high schools are as follows; 1) Science teacher's backgrounds for their teaching profession and majors are good, but they are overburdened and the classes are overcrowded. 2) The textbooks now in use contain too much contents, the levels are high, and the books lack in appropriateness. 3) Especially the contents Presented for experimental activities require too many tasks to be digested in a given time. 4) Experimental facilities or instruments are unsatisfatory in their quantity or quality, and instruments or teaching materials that can be used effectively are far from being sufficient. Futhermore, some experimental instruments are too crude to work successfully. 5) Students' elementary knowledge is not sufficient, and the degrees of their interest or understanding are low. 6) With the teaching methods still conservative and the teachers unable to give satisfactory inquiry leaning. the students are disencouraged. 7) Support of experimental facilities, encouragement of teachers, appropriate school inspection, and administrative or financial aids are desired. In this study, materials for every branch of science have been developed to be available for the effective performance of experiments and practices through inquiry. The materials developed in this study are as follows. 1. The following materials are presented in physics: the relations between the heat quantity of water and the temperature change, and between the heat quantity of the electric heat line and the electric current, for group experiments; the observation of Brown motion, Law of Archimedes, altimeters, comparing the heat capacity of metals, the transmission of radiation, the frictional electricity, the interaction of electric current with magnetic needle, and Fleming's right-hand rule, as the reference materials of demonstration; a program learning materials for diffusion of light with experimental kit utilized in learning the properties of light; a program learning material on the diffraction of light with an experimental kit utilized in learning the diffraction and interference of light. 2. In chenistry, the experimental materials for electrolysis are presented as the teaching-learning material of group experiment. Besides, the errors in the experimental materials and inappropriate expressions in the textbooks now in use are corrected and completed: 3. In biology, problems have been extracted by means of questionaires about the general learning contents and the analisis of Japanese textbooks, and the materials for experiment have been developed. The observation of somatic cell division, that of mold, and that of prothallium are presented as the materials for group experiment. Inquiry learning based on experiment and practice should be settled on the scenes of learning, and the suggestions are made to the effect as follows. 1) The quantity of learning should be reduced, and the levels lowered in the new textbooks. 2) The development and distribution of individual learning materials for the master learning are desirable. 3) The items for group experiment should be reduced to the minimum, and the thorough inquiry learning should be pursued, and the greater part should be replaced by the demonstrative experiments or audio-visual materials. 4) The experimental instruments should be manufactured and distributed on the national scale to ensure their solidity and accuracy. 5) The atmosphere in which inquiry learning is performed by the teacher's creative efforts is greatly needed, To support this conditioning, exhibitions of scientific works should be held continually, science museums operated, the research centers of science education cooperated, new materials for practice developed and studied, and the corporations for research of science education fostered. 6) The measures to lighten the teachers' burden and to encourage them should be contrived.

HID 램프 수명 연장을 위한 적응형 점화기 개발

박종연,조계현,송명석 江原大學校 産業技術硏究所 2004 産業技術硏究 Vol.24 No.A

The metal halide lamps are now widely used in the application and commercial lighting due to their attracting properties such as good color rendering and high efficiency. But, they have the serious problem of acoustic resonance for high frequency operation and they need the high voltage to ignite. So, they have not been applied to indoors. Over the past few years, a considerable number of studies have been conducted on the electronic ballast together with hot restarting and resonance phenomenon. But, very few attempts have been made with the adaptive ignition method according to the lamp state. In this paper, electronic ballast is proposed for metal halide lamps with an igniter for adaptive ignition. The proposed electronic ballast can generate different ignition voltages according to the arc tube state.

산딸나무 열매로부터 저밀도 지질 단백질(LDL) 산화 역제물질의 분리

이대영,송명종,김민정,정낙훈,정태숙,이윤형,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

산딸나무 열매를 100% MeOH로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 물로 용매 분획 하였다. 이 중 EtOAc 분획으로부터 silica gel과 octadecyl silica gel(ODS) column chromatography를 반복하여 1종의 lignan 화합물을 분리, 정제하였다. 화합물의 화학구조는 NMR, Ms 및 IR 등의 스펙트럼 데이터를 해석하여, (+)-pinoresinol (1) 로 동정하였다. 이 화합물은 산딸나무에서 처음 분리되었다. LDL-항산화활성 억제활성이 20㎍/mL 처리농도에서 85.1±1.3%로 나타났다. (+)-Pinoresinol was isolated from the EtOAc fraction of methanol extracts of the fruits of Cornus kousa Burg. The chemical structures of (+)-pinoresinol was determined by spectroscopy method, as well as 1D- and 2D-NMR (^(1)H-^(1)H COSY, HSQC and HMBC) spectroscopy. This compound was the first to be isolated from C. kousa Burg. Also, (+)-pinoresinol inhibited LDL-oxidation with the inhibitory activity of 85.1±1.3% at a concentration of 20 ㎍/mL.

애기마름(Trapa pseudoincisa S. et. Z.)으로부터 sterol 화합물의 분리 및 구조동정

양혜정,송명종,노영덕,김대근,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

애기마름을 80% MeOH로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 H_(2)O로 용매 분획 하였다. EtOAc 분획으로부터 silica gel과 octadecyl silica gel(ODS) column chromatography를 반복 실시하여 2종의 화합물을 분리하였다. 각 화합물의 화학 구조는 NMR, MS 및 IR 등의 스펙트럼을 데이터를 해석하여, stigmasterol과 stigmasterol 3-O-β-D-glucopyranosyl로 구조 동정하였다. 이화합물은 애기마름에서는 처음 분리되었다. Trapa pseudoincisa was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and H_(2)O. Two compounds were isolated from the EtOAc fraction through repeated silica gel and ODS column chromatographies. From the result of spectral data including NMR, MS, IR and GC/MS the chemical structures of the compounds were determined as stigmasterol (compound Ⅰ), and stigmasterol 3-O-β-D-glucopyranosyl (compound Ⅱ). They were isolated from Trapa pseudoincisa for the first time.

상황버섯(Phellinus linteus)으로부터 Ergosterol의 분리

류하나,유종수,송명종,이대영,김동현,노영덕,김인호,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

상황버섯을 80% MeOH 용액으로 추출하고, 추출물을 EtOAc, n-BuOH 및 물로 분배, 추출하였다. 이 중 EtOAc 분획을 silica gel 및 octadecylsilica gel(ODS) column chromatography로 정제하여 1종의 sterol 화합물을 분리하였다. 화합물의 화학구조는 NMR, MS 및 IR 등의 스펙트럼 데이터를 해석하여, ergosta-7,22E-diene-3β,5α,6β-triol(cerevisterol, 1)로 동정하였다. 이 화합물은 상황버섯에서 처음 분리 보고 되었다. Phellinus linteus was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and H_(2)O. The repeated silica gel and ODS column chromatographies of the EtOAc fraction led to the isolation of one sterol. From the result of spectral data including NMR, MS and IR, the chemical structure of the sterol was determined as ergosta-7,22E-diene-3β,5α,6β-triol (cerevisterol, 1). Compound 1 was isolated from the Phellinus linteus for the first time in this study.

상추(Lactuca sativa L.)의 ACAT 억제 Diterpenoid, Phytol : 식용 식물자원으로부터 활성물질의 탐색 Development of Biologically Active Compound from Edible Plant Sources

장태오,방면호,송명종,홍윤희,김지영,정대균,배동근,권병목,김영국,이현선,김인호,백남인 한국농화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.1

자발성 세균성 복막염을 일으킨 그람음성간균의 3세대 세팔로스포린 내성률 변화

방지환,송경호,박주경,박완범,김성한,김홍빈,김남중,오명돈,김의종,이효석,최강원 대한감염학회 2007 감염과 화학요법 Vol.39 No.3

The third generation cephalosporin is widely used in treatment of spontaneous bacterial peritonitis (SBP). Resistance to the third generation cephalosporin was associated with poor outcome in patients with SBP. Thus it is necessary to know the changes in resistance rate. We planned to investigate retrospectively on resistance rate of the third generation cephalosporin of gram negative bacteria isolated in patients with SBP, who visited Seoul National University Hospital between 1998 and 2006. The Clinical and Laboratory Standards Institute guidelines were applied for antibiotic susceptibility test. A total of 269 consecutive episodes of clinically and microbiologically proven SBP was identified during the study period and 209 cases were caused by gram negative organisms. Among 209 isolates, 22 (10.5%) showed resistance to the third generation cephalosporin. The prevalence of resistance was decreasing during the study period (P=0.014).