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식용식물자원으로부터 활성물질의 탐색-XX. 순무(Brassica campestris ssp rapa)뿌리로부터 지질화합물의 분리
방면호,이대영,한민우,오영준,정해곤,정태숙,최명숙,이경태,백남인,Bang, Myun-Ho,Lee, Dae-Young,Han, Min-Woo,Oh, Young-Jun,Chung, Hae-Gon,Jeong, Tae-Sook,Choi, Myung-Sook,Lee, Kyung-Tae,Baek, Nam-In 한국응용생명화학회 2007 Applied Biological Chemistry (Appl Biol Chem) Vol.50 No.3
순무 뿌리로부터 활성 물질을 분리 동정 하기 위하여 80% MeOH 수용액으로 추출하고 이를 여과, 감압 농축하여 MeOH 추출물을 얻었다. 이를 EtOAc분획, n-BuOH분획, $H_{2}O$분획으로 나누었으며, EtOAc분획과 n-BuOH분획에 대해 silica gel 및 ODS column chromatography를 실시하여 5종의 화합물을 분리 정제하였다. $^{1}H-NMR$, $^{13}C-NMR$, DEPT spectrum 및 Mass spectrum 등을 통하여 palmitic acid methyl ester(1), linolenic acid methyl ester(2), linoleic acid methyl ester(3), ${\beta}-sitosterol$(4), daucosterol(5)으로 구조를 결정하였다. The roots of Brassica campestris ssp rapa were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_{2}O$. From the EtOAc and n-BuOH fractions, five compounds were isolated through the repeated silica gel column chromatographies. From the result of spectroscopic data including NMR and MS, the chemical structures of the compounds were determined as palmitic acid methyl ester (1), linolenic acid methyl ester (2), linoleic acid methyl ester (3), ${\beta}-sitosterol$ (4) and daucosterol (5).
순무(Brassica rapa) 뿌리로부터 이차대사산물의 분리 및 동정
방면호,한민우,정해곤,정태숙,최명숙,이경태,백남인,이대영 한국식물생명공학회 2009 식물생명공학회지 Vol.36 No.1
In order to identify secondary metabolites, the root of Brassica rapa was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and H2O. From the EtOAc and n-BuOH fractions, four secondary metabolites were isolated through the repeated silica gel and octadecyl silica gel (ODS) column chromatographies. From the result of spectroscopic data including NMR and MS, the chemical structures of the compounds were determined as 4-(methoxymethyl)phenol (1), α-methoxy-2,5-furandimethanol (2), phenyl-β-D-glucopyranoside (3), and 2-phenylethyl-β-D-glucopyranoside (4). They were isolated for the first time from Brassica rapa. 순무 뿌리로부터 활성 물질을 분리 동정 하기 위하여 80%MeOH 수용액으로 추출하고 이를 여과, 감압 농축하여 MeOH추출물을 얻었다. 이를 EtOAc분획, n-BuOH분획, H2O분획으로 나누었으며, EtOAc분획과 n-BuOH분획에 대해 silica gel 및 ODS columnchromatography를 실시하여 4종의 이차대사산물을 분리 정제하였다. 1H-NMR, 13C-NMR, DEPT spectrum 및 mass spectrum등을 통하여4-(methoxymethyl)phenol(1), α-methoxy-2,5-furandimethanol(2), phenyl-β-D-glucopyranoside(3) 및 2-phenylethyl-β-D-glucopyranoside(4)로구조를 결정하였다. 이 화합물들은 순무에서는 처음 분리되었다
흰씀바귀 (Ixeris dentata forma albiflora)뿌리로부터 Flavonoid 화합물의 분리
방면호,송명종,한민우,양혜정,이대영,백남인 慶熙大學校 食糧資源開發硏究所 2007 硏究論文集 Vol.26 No.2
The root of Ixeris dentata forma albiflora was extracted with 80% aqueous methanol and solvent fractionated with ethyl acetate, n-butanol and water, successively. From the ethyl acetate and n-butanol fractions, two flavonoid compounds were isolated through the repeated silica gel column chromatographies. The chemical structures were determined as luteolin (1), luteolin-7-0-βD-glucopyranoside (2) through the intεrpretation of several spectral data including 2D-NMR.
Anti-osteoporotic Activities of Fucosterol from Sea Mustard (Undaria pinnatifida)
방면호,이대영,Nam-In Baek,Hong-Hee Kim,Min-Woo Han,Yoon-Su Baek,정대균 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.2
Samples of the edible seaweed, sea mustard (Undaria pinnatifida) were extracted with 80% aqueous methanol, and the concentrated extract was partitioned with ethyl acetate, n-butanol, and water, successively. Isolated fucosterol (1) from the ethyl acetate layers using repeated silica gel and octadecyl silica gel (C_18, ODS-A)column chromatography and identified it using spectroscopic data including NMR, MS, and infrared spectroscopy (IR). Osteoclasts are specialized multinucleated cells with bone resorbing activity. The induction of c-Fos and NFATc1transcription factors by receptor activator of nuclear factor κB ligand (RANKL) is a key event in osteoclast differentiation. Isolated and identification compound 1 was investigated in vitro for their anti-osteoclastic activity. Compound 1 inhibited osteoclastic differentiation in a dose-dependent manner without cytotoxicity. In addition,the inhibitory mechanisms of compound 1 were investigated. Compound 1 induced a clear reduction in the expressions of NFATc1 and c-Fos upon RANKL stimulation. In summary, compound 1 from sea mustard showed antiosteoporotic potential.
식용식물자원으로부터 활성물질의 탐색 XVI. -사자발쑥(Artemisia herba)의 전초로부터 sterol 화합물의 분리-
방면호,정해곤,송명종,유종수,정선아,이대영,김세영,정태숙,이경태,최명숙,백남인,Bang, Myun-Ho,Chung, Hae-Gon,Song, Myoung-Chong,Yoo, Jong-Su,Chung, Sun-A,Lee, Dae-Young,Kim, Se-Young,Jeong, Tae-Sook,Lee, Kyung-Tae,Choi, Myung-Sook,Baek, 한국응용생명화학회 2006 Applied Biological Chemistry (Appl Biol Chem) Vol.49 No.2
사자발쑥의 전초를 80% MeOH 용액으로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 물로 용매 분획 하였다. 이 중 EtOAc 분획과 n-BuOH 분획으로부터 silica gel과 octadecyl silica gel(ODS) column chromatography로 정제하여 4종의 sterol 화합물을 분리하였다. 각 화합물의 화학구조는 NMR, MS 및 IR 등의 스펙트림 데이터를 해석하여, ${\beta}-sitosterol$ (1), dehydroergosterol $5{\alpha},8{\alpha}-epidioxide$ (2), stigmasterol (3), daucosterol (4)으로 동정하였다. 이 화합물들은 사자발쑥에서 처음 분리되었다. Sajabalssuk (Artemisia herba) was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$, successively. From the EtOAc and n-BuOH fractions, four sterols were isolated through the repeated silica gel and ODS column chromatographies. From the results of physico-chemical data including NMR, MS and IR, the chemical structures of the sterols were determined as ${\beta}-sitosterol$ (1), ergosterol peroxide (2), stigmasterol (3) and daucosterol (4). They were the first to be isolated from Sajabalssuk (Artemisia herba).