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식용 식물자원으로부터 활성물질의 탐색-XXIV.- 식용 식물 추출물의 항암 활성 -
백남인,곽호영,권병목,송명종,이진희,양혜정,김대근,안은미 한국생약학회 2007 생약학회지 Vol.38 No.4
screening of anti-cancer activity for the MeOH extracts of 163 natural sources, which were registered as edibleplants by Korea Food & Drug Administration, exhibited 9 extracts to have significant inhibitory effects on farnesyl-proteintransferase (FPTase) and phosphatase of regenerating liver-3 (PRL-3). In order to confirm the inhibitory activity of these activeextracts, the activity assay was repeated for some fractions obtained from the active extracts using Medium Pressure LiquidChromatography (MPLC). Some fractions of Carya illinoensis, Chlorella vulgaris, Panicum miliaceum, Perilla frutescens, Ros-marinus officinalis showed over 50% inhibitory activity on FPTase as well as those of Capsella bursa-pastoris, ,C. vulgaris, Coix lacrymajobi, Myristica fragrans, P. miliaceum, R. officinalis did over 50% inhibitory activity on PRL-3.Key words edible plant, anti-cancer, FPTase, STAT3, PRL-3
Sophorose의 제조 -Ⅱ. 효모 (Torulopsis bombicola) 배양액 및 회화나무 (Sophora japonica)로부터 Sophorose의 제조
백남인,이미자,이유희,박종대,김해영,김신일 ( Nam In Baek,Mi Ja Lee,You Hui Lee,Jong Dae Park,Hae Yeong Kim,Shin Il Kim ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.2
A yeast, Torulopsis bombicola, was cultured in the media fortified with soybean oil as a additional carbon source for 7 days with reciprocal shaking. From the culture broth, sophorose-lipid was isolated and treated with alkali to afford sophorose. The sophorose contained in the medium was acetylated and isolated through silica gel column chromatography. The aceylated sophorose was hydrolyzed with 5% KOH at room temperature to give rise to sophorose. Meanwhile, the MeOH extracts obtained from the pod of Sophora japonica was solvent-fractionated with n-BuOH and H₂O, and butanolic layer was chromatographed on silica gel column to afford a flavonoide-glycoside. The glycoside was hydrolyzed with 0.02 N H₂SO₄, to yield sophorose.
식용 식물자원으로부터 활성물질의 탐색-1 고구마 (Ipomoea batatas Lam .) 괴근 주요성분의 분리
백남인,안은미,방면호,김해영 ( Nam In Baek,Eun Mi Ahn,Myun Ho Bang,Hae Yeong Kim ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.6
The MeOH extracts obtained from the tuber of Ipomoea batatas Lam. were solvent-fractionated with EtOAc, n-BuOH, and H₂O, respectively. From EtOAc fraction four different compounds were isolated through the repeated silica gel column chromatographies. From not only the results of NMR and MS data but also the adaptation of hydrolysis, methylation, and acetylation, the chemical structures of compounds were elucidated as resin glycoside, simonin I, β-sitosterol, and two kinds of unsaturated fatty acids.