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상추(Lactuca sativa L.) 유래 항동맥경화물질 규명 및 가공적성 실험을 통한 건강기능성 식품 시제품 개발
배동근 안양대학교 산업기술연구소 2004 自然科學硏究 Vol.11 No.1
우리나라 전역에서 전통적으로 식용 및 약용으로 사용되어온 상추에서 생리활성물질을 탐색한 결과 항동맥경화성이 발견되어 상추로부터 항동맥경화성을 갖는 물질을 분리·정제하여 물질의 구조규명을 한 결과 phytol로 밝혀졌다. 또한 이 상추유래 항동맥경화물질의 열안정성, 광안정성 등 가공적성을 연구하여 이를 토대로 최적의 건강기능성 식품의 모델에 대한 연구를 하여 항동맥경화물질을 함유한 건강기능성 시제품을 개발하였다. The anti-atherosclerotic substance originated from lettuce(Lactuca sativa L.) was extracted with MeOH and further partitioned by using EtOAc, n-BuOH and H_(2)O. The substance was determined as phytol by NMR and MS. The lettuce extract with anti-atherosclerotic activity was tested for processing characters such as heat and light stability. Food model was developed with the processing parameters of the lettuce extract. The extracts was finally processed as a functional food in a tablet form with other biologically active ingredients.
Pai, Tongkun 안양대학교 산업기술연구소 2001 自然科學硏究 Vol.8 No.-
The optimum condition for rat hepatocyte primary cell culture as a model system for studying the role of stearate on cholesterol metabolism was determined. Up to the cell concentration of 1.5x10^6 cells/well, there was a linear relationship (r = 0.96) between the cell number and the quantity of cellular protein recovered after the incubation. Stearate bound to BSA at high levels in the medium (>97%) at all stearate-BSA ratios. Therefore, it is concluded that the cellular protein (mg) can be used as the base unit to express the results and stearate-BSA ratio of 5.6 was determined to be the optimum concentration when considering the difficulty of handling the medium at higher ratios.
식용식물자원으로부터 활성물질의 탐색-XI. GC를 이용한 상추(Lactuca sativa L.)의 품종별 Phytol 함량 분석
송명종,김지영,정대균,배동근,김인호,백남인,방면호,이진희 한국응용생명화학회 2004 Journal of Applied Biological Chemistry (J. Appl. Vol.47 No.4
An easy, sensitive and rapid method for the analysis of phytol, a strong ACAT (Acyl-CoA: Cholesterol Acyltransferase) inhibitory diterpenoid, was established. The pre-treatment of sample for GC analysis of phytol includes aq. MeOH extraction, partition of the extracts between EtOAc and water, and filtration of organic phase through Supelclean LC-Si SPE tube. The GC analysis of phytol for a variety of Lactuca sativa was carried out on DB-5 capillary column with flame ionization detector (FID). The consistency in phytol contents according to each variety was not shown. Especially, Jeoktoma, as RS-2 type, showed the highest phytol content.
식용 식물자원으로부터 활성물질의 탐색-V. -상추(Lactuca sativa L.)의 ACAT 억제 Diterpenoid, Phytol-
장태오,방면호,송명종,홍윤희,김지영,정대균,배동근,권병목,김영국,이현선,김인호,백남인,Jang, Tae-O,Bang, Myun-Ho,Song, Myoung-Chong,Hong, Yoon-Hee,Kim, Ji-Young,Chung, Dae-Kyun,Pai, Tong-Kun,Kwon, Byung-Mok,Kim, Young-Kuk,Lee, Hyun-Sun,Kim 한국응용생명화학회 2003 한국농화학회지 Vol.46 No.1