정상 한국인에서 연령증가에 따른 혈장 Dehydroepiandrosteronesulfate ( DHEA-S ) 농도 및 DHEA - S / cortisol 비의 변화

박성우,유형준,임성희,최문기,유재명,박민숙,이권엽,박철수,김철홍,김현규,김두만,박규용 대한내분비학회 1997 Endocrinology and metabolism Vol.12 No.2

Backgroud: DHEA-S is the most abundant steroid hormone in circulation, and primarily secreted from the adrenal cortex, but its physiological role is little known. One of the characteristic features of DHEA-S is progressive decrement of plasma DHEA-S level with advancing age, in contrast, plasma levels of other adrenal hormones are not chaging or littie decreasing. To grasp the trends of plasma DHEA-S level and DHEA-S/cortisol ratio by age in healthy Korean, we measured the plasma DHEA-S levels and DHEA-S/cortisol ratios in healthy Korean. Methods: Healthy Korean(men: 99, women: 102, age range: 15-97 year old)were studied. Subjects were not taking drugs(such as glucocorticoid or androgenic medication) or cigarettes known to modify the plasma level of DHEA-S and cortisol, and had no evidence of hepatic, renal disease or hyperlipidemia as determined by serum lipid, bilirubin, SGOT, SGPT, BUN, creatinine. Data were analyzed by 10-year age group for men and women: i.e, 10-19, 20-29, 30-39, 40- 49, 50-59, 60-69, 70-79, 80-89 and 90 year or more. Plasma DHEA-S levels were measured by using a commercially available RIA kit with 125I labeled-DHEA-SO4(Coat-A Count DHEA-SO4), and for the measurement of plasma cortisol levels, commercial Gamma Coat TM[125I] Cortisol Radioimmunassay Kit was used. Results:. 1) In both men and women, plasma DHEA-S level showed high interindividual variation within the same age group. 2) There were individual sex differences in plasma levels of DHEA-S, in all age groups, plasma DHEA-S levels were significantly higher values for men than for women. 3) Maximum plasma DHEA-S levels(men; 237+-3.35 ug/dL, women; 108+-17.5 ug/dL) were at third decade in both men and women. 4) Both men and women showed the continuous decline in plasma DHEA-S level with age. These age-related decline was more prominent in men than in women(men; y= -3.152 * +292.6, r2= 0.8459, P$lt;0.05, women; y= -1.417 * +143.3, r2 = 0.7278, P$lt; 0.05). 5) As an index of aging, there was no stastical difference between DHEA-S and DHEA- S/cortisol ratio. Conclusion: In healthy Korean, there were high interindividual variation of plasrna DHEA-S levels. In both men and women plasma DHEA-S level was peak at third decade, and from when it declined progressively with age. These results suggest that although the reliability of single plasma DHEA-S measurement are limited, the decline of DHEA-S with advancing age might be a specific marker of endocrinologic hormonal milieu(aging index). Also, concerning to individual adrenal secreting capacity, we measured DHEA-S/cortisol ratio. But we did not found that plasma DHEA-S/cortisol ratio is superior to the plasma DHEA-S level as an aging index. (J Kor Soc Endocrinol 12:245-254, 1997)

성대마비환자를 대상으로 시행한 후두근전도검사의 임상적 유용성

최홍식,최영준,문상우,김태만,최홍식,최영준,문상우,김태만 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.2

전주시내 일부 유치원 아동의 요충 감염조사

문홍만 광주보건대학 1995 論文集 Vol.20 No.-

The auther study was incidence of Enterobius vermicularis infections amon the 354 Kindergarten pupils in Jeon-ju area from February to March, 1995. The method employed was Scotch-tape anal swab and the results were obtained as follow: 1. The infection rate of Enterobius vermicularis was 6.2% among 354 Kindergarten pupils. 2. The infection rate of Enterobius vermicularis according to age was the highest in 5 years old with 7.3% and the lowest in 7 years old with 3.7% 3. The infection rate according to sex was 6.7% in male and 5.6% in female, and the highest in male 5 years old with 7.9%, in female 4 years old with 6.7%, and the lowest in male and female, 7 years old with 4.5% and 2.7%.

열안정형 카테콜-O-메틸전이효소 유전자의 클로닝

홍경만,최용복,정갑용,지은정,장현신,박현,백문기 圓光大學校 醫科學硏究所 1998 圓光醫科學 Vol.14 No.2

Catechol-O-methyltransferase (COMT; EC 2.1.1.6) is the enzyme which catalyzes the transfer of methyl group to the catecholamine neurotransmitters from its methyl donor S-adenosyl-L-methionine. Partially purified COMT from rat liver has been used to measure the concentration of catecholamines in the blood through solvent extraction and thin layer chromatography after converting ^3H-methyl derivatives of catecholamines. To improve this inconvenient and fluctuating method, an attempt was made to use COMT gene for the measurement of catecholamines. Specific primers, COMT5P (5'-TGC TCA GAG GTG CTT TGA AG-3') and COMT3P (5'-GGA GCC GCA GAA GGT CA G-3'), were used to amplify COMT gene from human placenta cDNA library. The amplified COMT gene through 35 cycles of polymerase chain reaction was cloned into T-vector and the nucleotide sequences are determined by automatic sequencer. Human COMT has two common variants, a thermostable high activity form which has valine at amino acid 158 and a thermolabile low activity form which Has methionine at the same position. The cloned COMT gene in this study has both variants. Clone 4 and 5 have valine at amino acid 158 (guanine at nucleotide 472) and clone 1-3 have methionine at this site (adenine at nucleotide 472). In addition to this, there are other DNA polymorphisms in COMT gene at nucleotide 101, 102 (amino acid 34, a structural mutation cysteine/serine) and nucleotide 186 (a silent mutation). All of the clones have cysteine at amino acid 34 (guanine and adenine at nucleotides 101 and 102) and cytosine at nucleotide 186 except clone 3 which has thymine at this nucleotide. Clone 4 and 5 are thermostable high activity variants, suggesting being more useful for the measurement of catecholamines after expressing the gene.

광주시내 일부 유치원 아동의 장내 기생충 감염과 환경조사 연구

문홍만 광주보건대학 1983 論文集 Vol.8 No.-

The auther studied the incidence of intestinal parasitic infections and surveyed the environment of 485 Kingdergarten pupil fom May to September, 1982. The method employed was cellophane thick smear and the results are was follows: 1. The prevalence rate of intestinal parasites of total was 9.2% among 485 Kingdergarten pupil. The infection rate of Ascaris lumbricoides was 4.9%, Trichuris trichiura 3.0%, Enterobius vermicularis and Hymenolepis nana each 0.6%. 2. The infection rate of intestinal parasites occording to age was the highest in 6 years old with 10.6% and the lowest in 5 years old with 5.5%. 3. The infection rate of intestinal parasites occording to degree of father's educations was the lowest in the college group with 5.9% and the highest in the high school group with 17.6%, and the degree of mother's educations was the lowest in the college group with 8.3% and the highest in the middle school group with 10.5%. 4. The infection rate of intestinal parasites occording to occupations was the highest in office man with 12.9% and each 12.6% in public officer, 8.6% in commerce, 4.7% in transportation business, 3.1% in teacher and 0% in docter respectively. 5. The infection rate of intestinal parasites by number of children was the highest in the family of over 5 children with 14.2% and the lowest in 1 chileren with 2.7%.

家兎의 過 Cholesterol에 대한 미역의 영향

文洪萬 광주보건대학 1980 論文集 Vol.5 No.-

In order to observe the experimental hypercholesteremia of rabbit, the results treated with Undaria Pinnatifida (1,000㎎/㎏. body weight) were summarized as follows: 1. In the Control group, as the health rabbits were treated with the only cholesterol (500㎎/㎏. body weight), it showed the high hypercholesteremia from normal value and the thick of arterial wall in the lung was observed. 2. In the first group, as the rabbits were treated with Undaria Pinnatifida from second weeks, continuosly increased cholesterol was decreased at once time but increased again. 3. In the second group, as the rabbits were treated with Cholesterol and Undaria Pinnatifida at the same time, the cholesterol was high increased but decreased to normal value and continuosly, and the thick of arterial wall in the lung was not observed.

제주 차귀도 연안해역 미생물 분포 및 이화학적 특성

문영건,김만철,이준백,송춘복,여인규,김종만,박근태,손홍주,최우봉,허문수 한국환경과학회 2006 한국환경과학회지 Vol.15 No.3

광주시내 일부 중학생의 장내 기생충 감염과 환경조사 연구

문홍만 광주보건대학 1985 論文集 Vol.10 No.-

The auther studied the incidence of intestinal parasitic infections and surveyed the environment of 1,347 middle school boy and girl from March to May, 1985. The method employed was cellophane thick smear and the results obtained are as follows: 1. The prevalence rate of intestinal parasites of total was 5.2% among 1,347 middle school and 6.6% in. school boy, 3.8% in school girl. The infection rate of Ascaris lumbricoides was 1.4%, Trichuris trichiura 3.1%, Hymenolepis nana 0.7%. 2. The infection rate of intestinal parasites according to school years was the highest in 2 school year with 6.3% and the lowest in I school year with 4.4%. According to sex in school years, I and 2 school years was the highest in school boy than school girl, but 3 school year was similar. 3. The infection rate of intinal parasites according to degree of education was the highest in the primary school group with 7.1% and the lowest in the college group with 3.8%. According to sex was the highest in the middle school group of school boy with 9.4%, the lowest in college group of school girl with 3.8%. 4. The infection rate of intestinal parasites according to occupations was the highest in Agriculture with 8.5% and each 7.0% in Public officer, 6.3% in Labor, 6.0% in. Transportation business, 5.4% in Nonoccupation, 3.6% in Office man, 2.1% in Teacher, and 0% in Doctor respectively. In the posotive, according to sex was the highest in school boy of Agriculture with 11.4%, the lowest in school girl of Office man with 1.3%. 5. The infection rate of intestinal parasites by number of children was the highest in the family over 5 children with 5.7% and the lowest in I children with 0%. In the positive, according to sex was the highest in school boy of over 5 children with 8.5% and the lowest in school girl of 3 children with 2.4%.

Helicobacter pylori가 만성위염과 위종양에 미치는 영향에 관한 면역조직화학적 밑 초미세구조적 연구

문경래,정순봉,이미숙,이미자,서재홍,김만우,박찬국 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Background and Objectives : Helicobacter pylori (H. pylori) is accepted as a principal cause of chronic gastritis and an important factor in the pathogenesis of peptic ulcer and gastric malignancy. The aim of our studies is to investigate relationships between H. pylori and gastric epithelial cells. Materials ðods : One hundred and eighty one H. pylori positive cases of gastric biopsy specimens were studied immunohistochemically and ultrastructurally. Results : p53 positive staining exhibited foveolar epithelial hyperplasia in 1 out of 32 cases, regeneration in 2 out of 12 cases, incomplete intestinal metaplasia in 5 out of 41 cases, complete intestinal metaplasia in 3 out of 18 cases, chronic ulcer in 1 out of 9 cases, tubular adenoma in 2 out of 14 cases and intestinal type of adenocarcinoma in 16 out of 21 cases. Ultrastructurally, H. pylori was observed to be in direct contact with the microvilli of the target cells and was demolishing the surface microvilli. The organisms were in close contact with the uncoated cell membrance. Penetration into the gastric cells by a few organisms was associated with marked cell damage and ultimately to cell disintegration. Conclusion : Our results suggest that H. pylori plays a role in the pathogenesis of gastric cancer, leading a relatively slow, long lasting process that induces chronic active gastritis, intestinal metaplasia, dysplasia, and adenocarcinoma of intestinal type.

백서에서 신 허혈성 손상에 미치는 칼슘 길항제의 효과

문철웅,정종훈,박천국,이승일,배학연,장경식,김만우,정춘해,홍순표,이병래,김호중 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

Renal ischemia is one of the most common causes of acute renal failure. Four factors related to the pathogenesis of acute renal failure are vasoconstriction, decreased glomerular filtration rate, tubular back leak of filtrate, and intratubular obstruction. The cellular response to renal ischemic insults include decreased content of adenosine trihosphate, lipid peroxidation induced membrane degradation, alteration in cellular pH, and calcium or phospholipase induced mitochondrial dysfunction. Much attention has been given to the role of increased cellular calcium as a pathogenetic contributor to cell injury during ischemia. Author studied the protective effects of calcium antagonists on cellular injury during renal ischemia in rat. To investigate the protective role of these agents, author measured the amount of malondialdehyde(MDA) and the enzyme activities of free radical scarvengers-superoxide dismutase(SOD), catalase and glutathione peroxidase from non-pretreated group and calcium antagonists pretreated groups in control, ischemia and reflow subgroups. The results were summerized as follows: 1) The amount of MDA in non-pretreated group was higher in the reflow compared with the control(<p<0.01). But, in all pretreated groups, there was no statistically difference in the amount of MDA. 2) The SOD activity in non-pretreated group was lower in both the ischemia and the reflow compared with the control (P<0.05). But, in both verapamil and trifluoperazine-pretreated groups, there was no statistically difference in the SOD activity. 3) Both catalase and glutathione peroxidase activities in non-pretreated group were lower in both the ischemia and the reflow compared with the control (P<0.05). But in all pretreated groups, there was no statically difference in both catalase and glutathione peroxidase activities. These results suggest that free radical mediated ischemic injury by renal artery clamp in rat can be protected by intraperitoneal pretreatment with calcium antagonists. As trifluoperazine has a protective effect in renal ischemia, the calcium activated calmodulin dependent enzyme may play a role in renal ischemic injury.