http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Rhizopus nigricans 를 이용한 고농도의 Progesterone으로부터 11α- hydroxyprogesterone의 생산
최용복,최상기,김학성,박영훈 한국산업미생물학회 1990 한국미생물·생명공학회지 Vol.18 No.1
부신피질호르몬 제조의 중요한 전구체인 11α-hydroxyprogesterone을 Rhizopus nigricans를 이용하여 고농도의 progesterone으로부터 고수율로 생산하기 위한 연구를 수행하였다. 생물전환수율을 최대로 하는 배지조성은 포도당과 casamino acid의 비율이 2:1인 것으로 관찰되었다. Progesterone은 Tween 80 용액에 분산시켜서 종균배양후 12시간 정도 지나서 첨가하였을 때 전환수율이 높았다. Progesterone의 농도가 증가함에 따라 생산성이 증가하였고 progesterone이 20g/l일 경우 전환수율은 약 70% 정도였다. A study on 11 α-hydroxylation of progesterone by using Rhizopus nigricans was carried out to produce efficiently 11 α-hydroxyprogesterone which is an essential intermediate of corticosteroids synthesis. Firstly, medium was optimized in view of bioconversion yield and cell growth. Glucose and casamino acid were selected as carbon and nitrogen source and the ratio of carbon to nitrogen which maximize bioconversion yield was determined to be 2:1. Secondly, the addition time of progesterone and dispersion method were studied. When progesterone dispersed with 0.01%(v/v) Tween 80 was added at 12-14 hr of cultivation, higher bioconversion yield was obtained. When 20g/l of progesterone was added, the yield reached upto 70% under optimized conditions.
Increased lysine N-methylation of a 23-kDa protein during hepatic regeneration
최용복,고명현,신창호,김경숙,홍경만,백문기,박동은 생화학분자생물학회 2005 Experimental and molecular medicine Vol.37 No.3
The methylation of a 23-kDa nuclear protein increased after partial hepatectomy and methylation returned to basal levels after the initial stage of regeneration. The methylating enzyme was partially purified from rat liver by ammonium sulfate precipitation, DEAE-anion exchange chromatography and Butyl-Sepharose chromatography. The 23-kDa protein was purified from a nuclear fraction of liver tissue with SP-Sepharose. When the 23-kDa protein was methylated with the partially purified methyltransferase and analyzed on C18 high performance liquid chromatography (HPLC), the methylated acceptor amino acid was monomethyl lysine (MML). Previously, only arginine N-methylation of specific substrate proteins has been reported during liver regeneration. However, in this report, we found that lysine N-methylation increased during early hepatic regeneration, suggesting that lysine N-methylation of the 23-kDa nuclear protein may play a functional role in hepatic regeneration. The methyltransferase did not methylate other proteins such as histones, hnRNPA1, or cytochrome C, suggesting the enzyme is a 23-kDa nuclear proteinspecific lysine N-methyltransferase.
Progesterone의 연속첨가에 의한 11$\alpha$-hydroxyprogesterone으로의 생물전환수율의 증대
최용복,김학성,박영훈 한국미생물 · 생명공학회 1990 한국미생물·생명공학회지 Vol.18 No.3
Biotransformation of progesterone to 11 $\alpha$ -hydroxyprogesterone by growing cells of Rhizopus nigricans was investigated. As the concentration of progesterone increased, the specific growth rate of R. nigricans decreased linearly, and consequently the conversion yield lowered. The hyphae of the microorganism were observed to become thicker, shorter, and more densely branched at high concentrations of progesterone. In order to improve the process productivity, biotransformation was conducted with continuous addition of progesterone. When the substrate was added continuously at a rate of 0.86 g/hr for 30 hrs, overall conversion yield reached upto 56% while a single addition of the same amount of progesterone yielded about 40% eonversion. When additional feeding of glucose was carried out upon its depletion, an improved br'oconversion yield upto 68% was obtained.
Rhizopus nigricans를 이용한 고농도의 Progesterone으로부터 11$\alpha$-hydroxyprogesterone의 생산
최용복,최상기,박영훈 한국미생물 · 생명공학회 1990 한국미생물·생명공학회지 Vol.18 No.1
부피질호르몬의 제조의 중요한 전구체인 11alpha-hydroxyprogesterone을 Rhizopus nigricans를 이용하여 고농도의 progesterone으로부터 고수율로 생산하기 위한 연구를 수행하였다. 생물전환수율을 최대로 하는 배지조성은 포도당과 casamino acid의 비율이 2:1인 것으로 관찰되었다. Progesterone은 Tween 80 용액에 분산시켜서 종균배양 후 12 시간 정도 지나서 첨가하였을 때 전환수율이 높았다. Progesterone의 농도가 증가함에 따라 생산성이 증가하였고 Progesterone이 20g/$\ell$일 경우 전환수율은 약 70정도 였다. A study on 11 $\alpha$-hydroxylation of progesterone by using Rhizopus nigricans was carried out to produce efficiently 11 $\alpha$-hydroxyprogesterone which is an essential intermediate of corticosteroids synthesis. Firstly, medium was optimized in view of bioconversion yield and cell growth. Glucose and casamino acid were selected as carbon and nitrogen source and the ratio of carbon to nitrogen which maximize bioconversion yield was determined to be 2:1. Secondly, the addition time of progesterone and dispersion method were studied. When progesterone dispersed with 0.01% (v/v) Tween 80 was added at 12-14 hr of cultivation, higher bioconversion yield was obtained. When 20g/$\ell$ of progesterone was added, the yield reached 70% under optimized conditions.
濟州國際自由都市의 生態的 特性을 고려한 生態觀光 活性化 方案 硏究
최용복 국토연구원 2003 국토연구 Vol.36 No.-
Korean government has initiated the special law for Jeju International Free City in order to build Jeju-Do as an international city focusing on tourism and leisure. Even though Jeju has been recognized as the best favorite tourism site possessing the preserved natural resources in the nation, it faces a risk due to the lack of tourism programs and efforts to develop tourism resources. Indeed, since the trends of tourism development have been oriented in a large scale development that caused to a serious destruction of natural environment, social conflicts between developmentalists and environmentalists have been aroused. In these circumstances, ecotourism has been emerged as an alternative tourism that minimizes its impacts upon environment and emphases on ecological sustainability. In this sense, the purpose of this paper is to propose the development alternatives for tourism development under the assumption that ecotourism is the best suitable tourism type for Jeju island. In order to fulfill this purpose, following three subjects have been investigated and analyzed : 1) status of ecotourism with a wide examination of ecological characteristics of Jeju, 2) analysis on recognition of tourists and citizens regarding ecotourism development, and 3) the possible development alternatives extracted from a comprehensive understanding and examination of 1) and 2).