Ethylenediamine-N, N'-di-S-α-Propionic Acid의 Alanine과 Cysteine 코발트(Ⅲ) 착물

전무진,박윤봉,최승락 연세대학교 자연과학연구소 1986 學術論文集 Vol.17 No.-

△-s-cis-H[Co(SS-eddp)Cl_2]H_2O 착물과 amino acid인 alanine 또는 cysteine과의 치환반응을 통하여 새로운 amino acid의 코발트(Ⅲ) 착물을 합성하였다. Alanine과 dichloro코발트(Ⅲ) 착물의 치환반응을 통하여 s-cis-[Co(SS-eddp)(S-ala)] 착물을 얻었는데 이는 치환반응 과정에서 어떠한 isomerization도 일어나지 않았음을 보여준다. 새로이 합성한 이 착물의 기하학적 구조는 전자 흡수 스펙트럼과 핵 자기 공명 스펙트럼으로부터 결정하였다. 한편 cysteine은 alanine과 달리 코발트(Ⅲ) 금속에 배위할 수 있는 세개의 작용기를 가지고 있는데 dichloro코발트(Ⅲ) 착물과의 치환 반응 과정에서는 N과 S가 주게원자로 작용하여 cysteinato코발트(Ⅲ) 착물을 형성하였다. A series of new cobalt(Ⅲ) complexes of amino acid with SS-eddp have been prepared via the substitution reactions of dichloor cobalt(Ⅲ) complexes of SS-eddp with S-alanine and S-cysteine. These complexes have been characterized using IR, electronic absorption spectra, and pmr spectra. The coordination mode of cysteine in [Co(SS-eddp)(cy)]^- is through amino and mercaptide groups whereas S-alanine is coordinated through amino and carboxylate groups in [Co(SS-eddp)(S-ala)].

Ethylenediamine-N, N'-di-α-isobutyric Acid의 Cobalt(Ⅲ) 착물의 합성

전무진,박장우,최승락,박광하 연세대학교 자연과학연구소 1987 學術論文集 Vol.18 No.-

주게전자가 ONNO인 네자리 리간드, ethylenediamine-N, N'-di-α-isobutyric acid(eddib)를 합성하고 이 리간드의 디클로로 코발트(Ⅲ) 착물을 공기산화법에 의하여 합성하였으며 이 착물의 uns-cis이성체와 s-cis이성체를 이온 교환 크로마토그래피법에 의하여 분리하였다. 그리고 eddib의 디클로로 코발트(Ⅲ) 착물을 용액중에서 Co(eddib)L^n+(L=Cl·H_2O,CO_3^2-, (H_2O)_2)로 치환반응을 시켰을때 이들의 전자흡수 스펙트럼의 흡수띠 위치가 분광학적 계열과 일치함을 알 수 있었다. A new flexible N_2O_2-type tetradentate ligand, ethylendiamine-N, N'-di-α-isobutyric acid (eddib), has been synthesized, and dichloro cobalt (Ⅲ) complex of eddib has been prepared via the airation method. The geometric isomers of the complex, s-cis and uns-cis isomers, have been separated through ion exchange chromatographic method. Aseries of cobalt (Ⅲ) complexes of eddib, [Co(eddib)L]^n+ (L=Cl. (H_2O), Co_3^2-, (H_2O)^2) have been prepared in solution. In their electronic absorption spectra, the absorption maxima and their intensities of the above series of complexes are on the ordinary line of the spectrochemical and hyperchromic series. Elemental analysis, IR, NMR and electronic absorption spectra have been used to characterize the complex and geometries of the complex.

Ethylenediamine-N,N'-di-α-Butyric Acid의 로디움(Ⅲ) 착물

전무진,한창윤,박장우,최승락,박광하 연세대학교 자연과학연구소 1985 學術論文集 Vol.15 No.-

네자리 리간드인 ethylenediamine--N,N'-di-α-butyric acid(eddb)의 디클로로 로디움(Ⅲ)착물을 합성하고 크로마토그래피법으로 분리하였다. Eddb리간드가 로디움(Ⅲ) 이온에 배위될 때 s-cis와 uns-cis기하 이성체가 형성됨이 관찰되었다. 합성된 착물의 구조는 적외선 분광분석법, 핵자기 공명 및 흡수 스펙트럼으로 확인되었다. Dichloro rhodium(Ⅲ) complex of a flexible tetradentate ligand of ethylenediamine-N,N'-di-α-butyric acid(eddb) has been prepared and separated by the chromatographic method. S-cis and uns-cis geometric isomers have been yielded during the preparation of complex. IR, PMR and electronic absorption spectra have been used to characterize the complex.

급성 충수염으로 진단된 예측 불가 염증성 맹장 종물에 대한 외과적 고찰

민경진,백무준,채만규,김성용,이문수,김창호,김재준,송옥평 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.1

Purpose: An unexpected inflammatory cecal mass of uncertain etiology was encountered during surgery for presumed appendicitis. In this case, the surgeon couldn't exclude differential diagnosis which were severe appendicitis involving the cecal region, diverticular disease, other inflammatory bowel diseases, and neoplasm. Therefore, this finding leads to a therapeutic dilemma for the surgeon, when making surgical decisions during the operation. The aim of this study was to resolve this dilemma, a retrospective review was undertaken in this study. Methods: A retrospective study was performed to review the management of this problem at Soonchunhyang University Chunan Hospital for eight and half years from July 1991 to December 1999. A review of the emergency operation records identified 50 patients who had undergone ileocecal resection or right hemicolectomy for an inflammatory ileocecal mass of uncertain etiology when operated on for probable appendicitis. Results: Altogether, 39 patients underwent ileocecal resection, and 11 patients underwent right hemicolectomy. The final pathologic diagnosis was appendiceal abscess in 32 patients (63%), cecal diverticulitis in 7 patients (14%), neoplasm in 6 patients (12%), appendiceal phlegmon in 3 patients (6%), ileal tuberculosis in 1 patient (2%) and Anisakiasis in another patient (2%). The neoplasm of the 6 neoplasm patients was ascending colon cancer for 4 patients and cecal cancer for 2 patients. There was no mortality in this group. Conclusion: Although most inflammatory cecal masses are caused by benign disease, all cases in which the intraoperative diagnosis is unclear, any pathologic diagnosis including neoplasm cannot be ruled out. So this suggest ileocecal resection or right hemicolectomy to remove a possible underlying malignancy, and to decrease morbidity and mortality.

생체분해성 고분자를 이용한 사시수술용 제형개발 및 효용성에 대한 연구

민병무,김용백,김승영,김창식,박근성,길숙종,조항진,이성복,노승무,송규상,강대영,조준식,양준묵,정경수,최선웅,이진호,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

A new device, muscle clamping system was developed to facilitate exact quatifying technique A strabismus surgery, and reduce the risk of complications. The device is composed of a lower fixing body with three jaws and an upper supporting body. They are used to clip an extraocular muscle and fix it to the sclera with a single bite. Superior rectus recession on 16 rabbit eyes were performed with this new device. Conjunctival injection, muscle adhesion strength, and light microscopic findings were examined at 1, 2, 4 and 8 weeks postoperatively. The Conjunctival injection were minimal, adhesion power ranged from 420 to 600 gram gravity, which is sufficient in withstand the normal pull of human extraocular muscle. In microscopic exam, some Inflammatory cells and fibrosis were found. The new device was technically easy, fast, and accurate, so it may be useful in stabismus surgery.

교사의 스포츠 인식 및 참여실태에 관한 연구

차봉준,안영필,이무진 東亞大學校 附設 스포츠科學硏究所 1997 스포츠科學硏究論文集 Vol.15 No.-

A teacher's sport activities have influence on student's physical, mental and emotional soundness. It is essential that study for teacher's sport activities should be conducted, so I under took this stud. This reserch's object based on recognition for sports, sports participation and degree of sport participation. To accomplish this reserch's object, I collected and classified data by agoe, sex, place was 281 persons. The following and conclusion after credible sratistic verfication. 1. An attitude for teacher's sports show positive inclination, but women more negative than men in regarding sports activites. 2. Men and women don't take part in sports activities for time space, talent, lack of interest, individual physical condition, lack of interest, individual physical condition, lack of facility in turn. 3. Mountaion climbing was the most favorite item of sports, and many enjoyed bowling and swimming of leisurely individual sports. 4. Professional and semp-Pro events-Baseball, soccer, tennis, gymnastics, basketball- were favored as spectator sports. 5. In all levels of teacher's the time and expenditure for sports activities were shown very imsufficient. 6. Almost people spend about and hour a week for sports activities. Next frequency is form one hour to hours, nothing, above seven hours in turn. 7. For the improvement of school sports participation, school sports facilities should be enlarged first and the teacher's should posotively and volumtorily parbicipate in sports activites.

젖소 足皮膚炎에 대한 오존수의 治療 效果

이수진,조성환,전무형,김덕환,박창식,한홍율,김명철 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

This study was carried out to determine therapeutic the effect of ozonated water therapy on bovine pododermatitis. In addition, bactericidal effect of ozonated water on etiological agent of bovine pododermatitis was examined. The pathohistological examination for the pododermatitis, according to application with ozonated water and ozone ointment was investigated. Thirty healthy cattle were divided two groups(each of 15) : control group (povidone group), treatment group (ozone solution group). Various parameters were evaluated in terms of the lameness score, swelling score, lesion score, WBC count, neutrophil count, pathohistological finding, and antimicrobial action. The decrease of lameness and lesion score were shown in hoof lesions on 14 days after application of ozonated water. Significant decrease of swelling was shown in hoof lesions on 14 days 1 Corresponding author after application of ozonated water(p < 0.01). In hematological findings, WBC count revealed values within normal range. The number of neutrophils was slightly higher than that of normal, however, this was improved on 14 days after application of ozonated water. In pathohistological findings, recovery was rapid macroscopically and microscopically in the treatment with ozonated water on the hoof lesions and ozonated water was effective. In antimicobial action, bactericidal effect was observed in treatment with ozonated water on the hoof lesions and ozonated water was effective.

대장암종에서 암관련 유전자들의 Promoter Methylation과 암종 발생 및 진행과의 관게에 대한 연구

정동준,이정은,민영기,조성우,백무준,양승하,김의한,김창진 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

The colorectal corcinoma is one of the most common malignant tumor in Korea and its carcinogenic mechanism has not been elucidated exactly yet. So far, the most common carcinogenic mechanism has been known to be multiple genetic changes. But, the CpG island hypermethylation has been revealed to be one of the most important carcinogenic mechanism second to the genetic changes. The epigenetic changes characteristically imprint and transfer to the next generation without changes of nucleotide sequences. When the CpG island of promoter or 5'- exon 1 of a specific gene is methylated by DNA methyltrasferases (DNMTs), the gene expression is inhibited. Carcinogenic mechansisms by promoter methylation has been well known, but the progression of the cancer has not been elucidated yet. The molecular researches on the carcinogenic mechanisms largely depended on the tumor tissue containing various components of cell, due to difficulties in harvesting pure homogeneous tumor cells. Promoter methylatonof the cancer related genes as APc, hMLH2, p16, DAP-K and MGMT in 50 cases of sporadic colorectal carcinoma was studied. The pure normal colorectal epithelial cells, and cancer cells from superficial and deep invasive portion were harvested respectively by laser captured microdissection(LCM), and DNA was extracted from them. The promoter methylation was studied by methylation specific poymerase reaction (MSP) after bisulfite modification of the DNA and the expression of the genes by immunohistochemistry. The results suggests that promoter methylation of the cancer related genes are closely related with the colorectal carcinogenesis and it occurrs from early phase of carcinogenesis, progressing constantly to the late stage. And the promoter methylation of normal epithelial cells increased by age. The p15, known to be the genes related to hematologic malignancy, was closely related to the colorectal carcinoma as well.

젖소 足皮膚炎에 대한 오존연고의 治療 效果

이수진,전무형,조성환,김덕환,박창식,김명철 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

Thirty dairy cows with pododermatitis were selected and treatment effect of ozone ointment for bovine pododermatitis was investigated. In addition, bactericidal effect of ozone ointment on etiological agent of bovine pododermatitis was evaluated. The pathohistological examination for the pododermatitis, according to application with ozone ointment was investigated. Thirty dairy cows were divided two groups: control group(vaseline group: 15 cows), treatment group(ozone ointment group: 15 cows). Various parameters were evaluated in terms of the lameness score, swelling score, lesion score, WBC, neutrophil, pathohistological finding, and antimicrobial action. As compared with vaseline group, ozone ointment group revealed significant decrease of lameness (p<O.05), swelling (p<O.O1) and lesion score (p<O.05) were shown in hoof lesions on 14 days after application. In hematological findings, WBC count revealed slightly high values within normal range before treatment, however, this was I Corresponding author improved on 14 days after application of ozone ointment. The number of neutrophils was slightly higher than that of normal, however. this was improved on 14 days after application of ozone ointment. In pathohistological findings, normal dermal tissue was found in tissues with pododermatitis on 14 days after application of ozone ointment. In antimicobial action, marked decrease rate of bacteria was observed in feet of all cases treated with ozone ointment. The decreasing rate of bacteria in anaerobic culture was higher than that in aerobic culture.

Paraquat 중독환자의 초기검사로서 sodium dithionite를 이용한 소변내 paraquat검출의 임상적 의의

윤갑준,임경수,이진웅,김영식,이부수,박덕우,김선만,이강현,황성오,안무업 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Background : Mortality from paraquat intoxication depends upon plasma paraquat concentration. To know the severity of paraquat intoxication is important for directing therapeutic modality and predicting prognosis. Sodium dithionite test for urinary paraquat provides an easy and simple method to determine the severity of paraquat intoxication in emergency department. purpose : To determine whether the result urinary paraquat test by sodium dithionite can predict outcome in patients with paraquat intoxication in emergency department. Subjects : 48 patients(male 31, female 17, mean age 37 years) who had exposure to paraquat and presented within 24 hours after exposure. Result : Thirty five patients were positive in paraquat urine test and thirteen patients were negative. Clinical manifestations were more severe in positive patients than in negatives. Complication was much more in positives than in negatives. 28 of 35 patients(80%) in positives and 2 of 13 patients(15%) in negatives died. Conclusion : Positive test for urinary paraquat is associated with high mortality and morbidity from paraquat intoxication, and qualitative test for urinary paraquat by sodium dithionite is an useful method to determine the severity of paraquat intoxication in emergency department.