녹두 발아중 분획한 단백질의 전기영동 패턴의 변화

조성환,표광호 한국농화학회 1989 Applied Biological Chemistry (Appl Biol Chem) Vol.32 No.3

Changes of protein contents and amino acid composition and SDS-PAGE pattern of protein of mung bean which were germinated in dark at 25℃ for 6 day. The total protein contents gradually decreased during germination and the contents of each fractionative soluble proteins were increased shortly after the soaking of mung bean and gradually decreased during the germination afterwards. SDS-PAGE of albumin fraction showed 18 bands, and during the germination the most of bands were diminished or disappeared. But protein bands at 24,000, 40,000, 45,000, 70,000 dalton position remained until 6th day of germination. SDS-PAGE of globulin fraction showed 6 discrete bands, and during the germination the protein band at 45,000 dalton position disappeard. But the protein bands at 14,000∼25,000 dalton position did not change during the period. SDS-PAGE of globulin fraction showed 10 discrete hands, and during the germination the bands of 45,000∼70,000 dalton become diminished or disappeared. But the bands of 30,000, 60,000 dalton did not change throughout the germination period.

Evaluation of Pavement Responses under Wide Base Tire and Dual Tire Assembly

조성환,임정혁 한국도로학회 2014 한국도로학회논문집 Vol.16 No.2

PURPOSES : The first generation of wide base tires introduced in the early 1980s was found to cause a significant increase in pavement damage compared to dual-tire assemblies. However, wide base tires have evolved considerably, and a new generation of wide base tire is thought to be comparable to conventional dual tires for pavement damage. A challenge associated with using wide base tires is the accurate quantification of pavement damage induced by these tires. The objective of this study was to investigate the responses of flexible pavement to continuously moving vehicular loading under various tire configurations. METHODS : The comparison of the strain/stress responses of full-depth pavement caused by conventional dual tire assembly and new generation of wide-base tires was performed. The FE model incorporates linear viscoelasticity of asphalt material and continuous moving load using implicit dynamic analysis. RESULTS AND CONCLUSIONS : The result demonstrates that the new wide-base tires caused slightly more fatigue damage and less

개 담관세포암(膽管細胞癌)의 1예(例)

조성환,김덕환,김교준,권오덕,박노태,Cho, Sung Whan,Kim, Duck Hwan,Kim, Kyo Joon,Kwon, Oh Deog,Park, No Tai 충남대학교 농업과학연구소 1986 Korean Journal of Agricultural Science Vol.13 No.2

식욕부진(食慾不振) 및 복부팽만(腹部膨滿)을 충남대학교(忠南大學校) 농과대학부속가축병원(農科大學部屬家畜病院)에 의뢰(依賴)된 11년(年)된 암컷 잡종견(雜種犬)을 병리조직학적(病理組織學的)으로 관찰(觀察)한 바 담관세포암으로 진단(診斷)하였고 그 결과(結果)는 다음과 같다. 임상적(臨床的) 소견(所見)으로 복부팽만(腹部膨滿), 식욕부진(食慾不振), 호흡곤란(呼吸困難), 및 침울(沈鬱) 등(等)을 관찰(觀察)할 수 있었고, 혈중(血中) GOT, GPT, LDH 및 BUN등(等)은 정상치(正常値)보다 증가(增加)하였으며 총단백량 및 Albumin 은 약간 감소(減少)하였다. 육안적(肉眼的) 소견(所見)으로 간장(肝臟)은 종대, 표면(表面)에는 많은 백색(白色)의 꽃양배추양 결절(結節)이 있었으며 직경(直徑) 3~4cm의 낭형성(囊形成)이 있었다. 낭내(囊內)에는 황백색(黃白色)의 교상물질(膠狀物質)이 차 있었다. 해부학적(解剖學的)으로 본종양을 간내(肝內) 담관암으로 분류(分類)하였다. 조직학적(組織學的)으로 본종양은 관상암과 담관낭종암이 혼합(混合)되어 있었으며 관상암은 관상구조(管狀構造)가 특징(特徵)으로 내피세포(內皮細胞)는 입방형(立方形) 또는 원계형(圓桂形)이며 결합조직(結合組織)의 증식(增殖)을 볼 수 있었다. 그리고 담관낭종암은 단층(單層)및 중층입방형(中層立方形) 또는 국주형세포(國柱形細胞)로 덮혀 있었다. On 11-year-old female mixed-breed dog with chief complaints of existence of abdominal enlargement and anorexia was referred to the veterynary teaching hospital of Chungnam National University. The dog was diagnosed into cholangiocarcinoma by the result of the histopathological observation. The results observed are summarized as follows: The clinical signs were abdominal enlargement, anorexia, dyspnea and depression. There were marked increase in serum glutamic pyruvic transaminase(GPT), serum glutamic oxaloacetic transaminase(GOT), lactic dehydrogenase(LDH) and blood urea nitrogen(BUN). Serum total protein and albumin were slightly below normal. In gross finding, the liver was enlarged and friable with numerous white, cauliflower-like nodules over the surface and composed on cysts from 3 to 4 cm in diameter. The cystic lesions contained a yellow gelatinous material. Anatomically, this neoplasm was classified as intrahepatic bile duct carcinoma. Histologically, this neoplasm was combined tubular carcinoma with bile duct cystadenocarcinoma. The former were characterized by tubular structures lined by anaplastic cuboidal or columnar cells with diffuse fibrous stroma, and the latter by multiple cystic structures lined by simple and stratified cuboidal or columnar cells.

MEASURED ENERGY CONSUMPTION ANALYSIS OF A DISTRICT HEATING SYSTEM UNDER DIFFERENT TEMPERATURE CONTROL STRATEGIES

조성환,홍성기,최상호,M. ZAHEERUDDIN 대한설비공학회 2014 International Journal Of Air-Conditioning and Refr Vol.22 No.2

In this study, the secondary side measured energy consumption of a district heating system (DHS)with different hot water supply temperature control methods was compared. Two controlmethods were evaluated: an outdoor temperature reset control (OTRC) and an outdoor temperaturepredictive control (OTPC). While the OTRC strategy has been widely used for energysavings in DHS, the results show that the OTPC strategy resulted in higher energy savings. Ingeneral, the OTPC strategy maintained lower supply water temperature, and thereby reducedstandby losses and increased overall heat transfer rate to the heated spaces due to higher watermass flow rate to the spaces. During the actual energy consumption monitoring, the OTPCstrategy saved about 6.6% more energy compared to that of OTRC strategy. Also, it was foundthat under partial load conditions, such as during the daytime, the fluctuations in hot watersupply temperature with OTRC were more severe than OTPC strategy. Overall, the resultsshowed that the OTPC strategy gave better control and was more stable under full load andpartial load conditions.

초・중・고등학생 대상 세계시민교육 프로그램에 대한 체계적 문헌고찰

조성환,김기덕,백예은,김재은 한국교육학회 2023 敎育學硏究 Vol.61 No.4

This study conducted a systematic review on global citizenship education (GCE) programs for Korean students in elementary, middle, and high schools. A total of 44 articles published in Korean journal from 2011 to 2022 were analyzed based on the framework: (1) approaches to delivery (UNESCO, 2015), (2) perspectives on GCE (Na & Jho, 2017), (3) research methods. The outcomes are as follows. First, GCE programs was mainly delivered by approaches of ‘integrated within certain subjects’ and ‘separate, stand-alone subject’, while few programs were delivered by ‘school-wide approach’ and ‘cross-curricular approach’. Second, while many GCE programs were delivered by ‘tourist perspective’ and ‘humanitarian perspective’, there were few studies based on ‘Neo-liberal perspective’ and ‘critical perspective’. Third, one out-dated scale has been used up to recently. And it was difficult to consistently interpret the effects of GCE program because different scales were used for each study. In addition, many studies did not report verification procedures about reliability and validity in the process of reorganizing questionnaire. Also, many did not use rigid research methods to analyze the effects of GCE program. Based on these findings, suggestions for future research related to GCE program were presented. 본 연구는 체계적 문헌고찰을 통해 초・중・고등학생을 대상으로 실행된 세계시민교육 프로그램 관련 연구물을 분석하였으며, 향후 세계시민교육의 실천과 후속연구를 위한 시사점을 제공하고자 하였다. 이를 위해 2011년부터 2022년까지 등재지 및 등재후보지에 출판된 44편의 국내 문헌을 선정한 뒤, 다음과 같은 분석 틀-(1) 세계시민교육의 실행 방식(UNESCO, 2015), (2) 세계시민교육의 관점(나장함, 조대훈, 2017), (3) 프로그램의 연구 방법-을 기준으로 문헌의 전문을 검토하였다. 분석 결과 및 제언은 다음과 같다. 첫째, 세계시민교육의 실행 방식을 분석한 결과, 대부분의 연구가 부가적 접근법인 ‘특정 교과에 포함된 주제’와 ‘독립 교과’ 방식을 적용하였으며, ‘전학교적 접근’과 ‘범교과 주제’ 방식의 세계시민교육 연구는 상대적으로 적었다. 둘째, 세계시민교육의 관점을 분석한 결과, 연구의 대부분이 ‘여행자적 관점’과 ‘인도주의적 관점’을 반영하였으며, ‘신자유주의적 관점’과 ‘비판적 관점’의 연구는 상대적으로 적었다. 셋째, 프로그램의 연구 방법을 분석한 결과, 많은 연구가 개발된 지 오래되고 타당성이 부족한 척도를 활용하여 프로그램 효과를 분석하고 있었다. 또한, 연구마다 상이한 척도를 사용하여 세계시민교육 프로그램 연구의 효과를 일관성 있게 해석하기 어려웠으며, 문항을 재구성하는 과정에서 신뢰도와 타당도 검증 절차를 보고하지 않은 연구들이 많았다. 그리고 엄격한 연구 방법을 사용하여 프로그램 효과를 분석한 연구과 그렇지 않은 연구를 분석하였다. 앞선 연구 결과를 토대로 세계시민교육 프로그램 관련 연구를 위한 제언을 제시하였다.

Genome-Wide Analysis of Genes Induced by Fusarium graminearum Infection in Resistant and Susceptible Wheat Cultivars

조성환,이정관,정기홍,이인원,Jong-Chul Park,백남천 한국식물학회 2012 Journal of Plant Biology Vol.55 No.1

Fusarium head blight (FHB) caused by Fusarium graminearum is one of the most serious diseases in wheat (Triticum aestivum) and barley (Hordeum vulgare). Dahongmil is an elite Korean wheat cultivar with relatively high resistance to FHB. To identify differentially expressed genes in the resistant cultivar Dahongmil and the susceptible cultivar Urimil after inoculation of F. graminearum, we used the Affymetrix GeneChip® Wheat Genome Array to identify 328 ESTs that were differentially expressed in inoculated seedling tissues of the two cultivars. From these, we selected 16 induced genes and found that they have defense functions, such as genes encoding pathogen resistance proteins, oxidative stress-related proteins, metabolism, and proteins involved in defense mechanisms. To verify the DNA microarray results, we tested seven of these genes by semiquantitative RT-PCR and confirmed that these defenseand stress-related genes were expressed at much higher levels in the resistant Dahongmil cultivar. We next developed a hypothetical functional gene network and identified 89interaction pairs mediated by four of the differentially expressed genes in the hypothetical network. We further refined the network by identifying nine genes showing significant up- or down-regulation after FHB challenge in the resistant cultivar and two genes having multiple interactions with queried proteins. We hope that the set of induced genes identified in this study can be used for development of new wheat and barley cultivars with improved resistance to FHB.

Effect of Natural Antioxidant Sources on Oxidation of Olive Flounder (Paralichthys olivaceus) and Fish Feed during Storage

조성환 한국수산과학회 2010 Fisheries and Aquatic Sciences Vol.13 No.3

The effects of various natural antioxidant sources on oxidation of olive flounder (Paralichthys olivaceus) and fish diet during storage was determined. Juvenile fish were distributed among 18 flow-through tanks (40 fish per tank). Six experimental diets were prepared in triplicate: control (CT), antitox (AT), green tea extract (GE), fig extract (FE), Haeroc product (HP) and by-product of green tea (BG). The experimental diets were stored at two temperatures: room temperature (26.8°C) for 14 days and frozen (-30°C) for 16 weeks. Thirty fish were sampled from each tank at the end of the 8-week feeding trial. Whole bodies of fish were homogenized and stored in a home freezer (-9.6°C) for 24 weeks. Acid values (AVs) and peroxide values (POVs) of the diets and frozen fish during storage were monitored. AVs of the experimental diets tended to increase with the storage period except for that of the HP diet at room temperature. POVs from FE, CT, and BG diets peaked at day 7 and then decreased through the remainder of the experiment. AVs of the experimental diets and fish increased with time at -30°C and -9.6°C. Results of this study show that by-products of green tea and Haeroc product seem to have potential as antioxidants in fish feed to inhibit oxidation of both the feed and fish during storage.

Regulation of CYP1A1 and Inflammatory Cytokine by NCOA7 Isoform 4 in Response to Dioxin Induced Airway Inflammation

조성환,박신영,이은정,조요한,박현선,홍석호,김우진 대한결핵및호흡기학회 2015 Tuberculosis and Respiratory Diseases Vol.78 No.2

Background: Aryl hydrocarbon receptor (AhR), a ligand-dependent transcription factor, binds to a wide variety of synthetic and naturally occurring compounds. AhR is involved in the regulation of inflammatory response during acute and chronic respiratory diseases. We investigated whether nuclear receptor coactivator 7 (NCOA7) could regulate transcriptional levels of AhR target genes and inflammatory cytokines in 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-treated human bronchial epithelial cells. This study was based on our previous study that NCOA7 was differentially expressed between normal and chronic obstructive pulmonary disease lung tissues. Methods: BEAS-2B and A549 cells grown under serum-free conditions were treated with or without TCDD (0.15 nM and 6.5 nM) for 24 hours after transfection of pCMV-NCOA7 isoform 4. Expression levels of cytochrome P4501A1 (CYP1A1), IL-6, and IL-8 were measured by quantitative real-time polymerase chain reaction. Results: The transcriptional activities of CYP1A1 and inflammatory cytokines were strongly induced by TCDD treatment in both BEAS-2B and A549 cell lines. The NCOA7 isoform 4 oppositely regulated the transcriptional activities of CYP1A1 and inflammatory cytokines between BEAS-2B and A549 cell lines. Conclusion: Our results suggest that NCOA7 could act as a regulator in the TCDD-AhR signaling pathway with dual roles in normal and abnormal physiological conditions.

동학의 자생적 근대성: 해월 최시형의 인간관과 세계관을 중심으로

조성환 서강대학교 서강대학교 신학연구소 2020 신학과 철학 Vol.- No.36

이 글은 동학의 근대성을 한국사상사의 맥락에서 찾고자 하는 시도이다. 종래에 동학의 근대성을 논한 선행연구들은 대체로 서구적 근대성을 기준으로 동학의 근대성을 찾고자 하였다. 이에 대해 본 논문에서는 조선시대와는 다른 새로운 사상적 요소를 동학의 근대성으로 규정하는 내재적 방법론을 취하였다. 그 중에서도 특히 동학의 인간관과 세계관에 나타난 새로움에 주목하였다. 동학을 창시한 최제우는 전통적인 ‘하늘’을 우주적 생명력으로 재해석하고, 그 우주적 생명력이 인간 안에 내재해 있다고 하는 시천주(侍天主)적 인간관을 제시하였다. 이어서 최시형은 그것을 ‘천인(天人)’ 개념으로 정식화함과 동시에 ‘하늘’의 의미를 우주적 생명력을 제공하는 천지(天地)로까지 확장시켰다. 그래서 최시형에게 있어 만물은 천지의 우주적 생명력을 먹고 자라는 하늘의 자식으로 자리매김된다. 이로 인해 인간의 모든 행위는 우주적 생명력의 외적 표현으로 간주되고, 신성한 행위로 긍정된다. 바로 여기에서 술(述)=해석에서 작(作)=창조로의 일대 전환이 발생하고, 모든 인간은 창조적 주체로 거듭난다. 종래의 ‘성인’의 자리를 자기 안의 ‘하늘’이 대체하게 된 것이다. 이것이 바로 개벽적 인간관과 성리학적 인간관의 가장 큰 차이이다. 또한 최시형은 우주를 도덕적 관점이 아닌 생태적 관점에서 재해석하여, 각각의 하늘들이 다른 하늘들에 자신의 존재를 빚지고 있는 ‘기화’의 그물망이라고 보았다. 그런 의미에서 이 세계는 일종의 ‘생태적 그물망’이라고도 할 수 있다. 이 생명연기의 세계에서는 어떤 존재도 홀로 존재할 수 없고, 타자와의 상호의존적 관계 속에서 자신의 생명을 유지하게 된다. 따라서 각각의 인간은 한편으로는 독립적이면서 다른 한편으로는 의존적인, 상반되는 속성을 동시에 지니게 된다. 이처럼 동학은 인간중심적이고 이성중심적이고 혈연중심적인 유학적 인간관과 세계관과 도덕관을 지구중심적이고 영성중심적이고 생태중심적인 것으로 전환시켰다. 그런데 이러한 경향들은 서구의 근대성보다는 탈근대성에 가까운 것들이다. 이후에 천도교에서는 동학적 인간관과 세계관을 바탕으로 서구의 근대성을 수용하게 되는데, 따라서 거기에는 서구의 근대성과 탈근대성이 혼재되게 된다. 바로 이 점이 동학과 천도교로 대표되는 한국적 근대성의 특징이다.

Effect of Fasting and Refeeding on Growth and Blood Chemistry in Juvenile Olive Flounder Paralichthys olivaceus L.

조성환 한국양식학회 2009 韓國養殖學會誌 Vol.22 No.1

Effect of fasting and refeeding on growth and blood chemistry of juvenile olive flounder Paralichthys olivaceus L. was investigated when fish achieved compensatory growth. Fish were fed the experimental diet for 6 days a week. Five treatments in triplicate were prepared: C, S1, S2, S3 and S4. Fish in the control group (C) were handfed to apparent satiation twice a day. Fish in treatments of S1, S2, S3 and S4 experienced 1, 2, 3 and 4 weeks of starvation and were then hand-fed to satiation twice daily during the remaining 7, 6, 5 and 4 weeks of the experiment, respectively. Weight gain of fish in C, S1 and S2 were higher than those of fish in S3 and S4. A significant difference in plasma total protein, glucose, triglyceride, T3 and T4 was observed in between starved and refed fish for the rest periods of the feeding trial. Plasma total protein and T3 of flounder decreased with week of fasting and following correlationships were obtained; Y (Total protein) = -0.13X (week of fasting) + 1.54, R2=0.9792 and Y (T3) = -11.48X (week of fasting) +79.57, R2=0.8822, respectively.