야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

결핵의 진단법으로서 중합효소 연쇄반응 검사의 가치

서상철,은상진,김한길,송경은,서장수,최성만,이원길,김재식,김중명 慶北大學校 醫科大學 1994 慶北醫大誌 Vol.35 No.1

목적 : 결핵균 동정을 위한 중합효소 연쇄반응을 임상병리검사로서 적용하기 위한 기초 실험으로 분석학적 예민도와 특이도를 알아보고 검토된 조건에 의한 중합효소 연쇄반응을 실제 임상 가검물에 적용하기 위함이다. 재료 및 방법 : Ziehl-Neelsen염색 양성인 객담, 뢰벤스타인-젠센(Loewenstein-Jensen) 배지에서 분리된 결핵균 및 M. tuberculosis H37Rv를 비롯한 항산균 표준균주 11주와 비항산균 10주를 사용하여 2가지 DNA의 분리법, 3set 프라이머, 3종류 중합효소, 2가지 미량 원심시험관 및 Easy-Cycler^R(Ericomp사, 미국)와 수조형으로 검사를 시행하여 DNA 증폭 산물을 비교하였다. 또한 이미 지일-닐슨 염색법에 의한 선별검사에서 음성을 나타낸 객담 53예, 소변 101예, 삼출액 128예, 뇌척수액 54예 등 총 370예의 임상가검물을 대상으로 하여 위에서 검토된 중합효소 연쇄반응 조건으로 검사한 성적과 지일-닐슨 염색법, 배양법 성적과 비교하였다. 결과 : DNA분리방법 2가지와 3가지 중합효소는 분석학적 예민도와 5fg로 나타나 차이를 발견할 수 없었으며, 프라이머 P1, P2쌍은 예민도가 5fg, 프라이머 INS1, INS2쌍과 프라이머 Pt3, Pt6쌍의 예민도는 0.5fg이었다. Easy-Cycler^R가 수조형에 비해 높고, 미량 원심시험관은 Robbins사 제품이 Sarstedt사 제품에 비해 높은 분석학적 예민도를 나타냈다. 프라이머 P1, P2쌍과 프라이머 INS1, INS2와 Pt3, Pt6 2쌍의 프라이머의 특이도에 있어서는 항산균 표준균주 중 M. tuberculosis H37Rv, M. tuberculosis ATCC 27294, M. bovis ATCC 29312의 3주에서 특이 증폭 띠를 관찰할 수 있었다. 그러나 비결핵성 항산균 8주에는 특이 증폭 띠를 나타내지 않았다. 또 비항산균 10주에도 특이 증폭 띠가 관찰되지 않았다. 따라서 Robbins사의 미량 원심시험관과 proteinase-K법에 의한 DNA분리방법에 Easy-Cycler^R를 사용하여 프라이머 INS1, INS2쌍으로 일차 중합효소 연쇄반응을 시행한 후 프라이머 Pt3, Pt6쌍으로 이차중합효소 연쇄반응을 시행하는 방법을 370예의 임상가검물에 시행하였고 지일-닐슨 염색법과 결핵균배양검사도 동시에 시행한 결과 중합효소 연쇄반응 양성은 106예(28.6%), 배양 양성은 27예(7.3%) 및 염색 양성은 18예(4.9%)였으며, 결핵환자는 141명(38.1%)였다. 370예의 임상 가검물로 시행한 중합효소 연쇄반응의 진단학적 예민도와 특이도, 양성결과 예측치 및 음성결과 예측치는 68.8%, 96.1%, 91.5% 및 83.3%로 각각 나타났다. 결론 : 결핵균 동정을 위한 중합효소 연쇄반응은 배양검사에 비해 신속하며, 분석학적인 예민도와 특이도가 뛰어나므로 결핵 진단을 위한 임상병리검사로서 유용하다고 사료됨. In this study, We investigated the optimal conditions of polymerase chain reaction(PCR) assay for the rapid identification of Mycobacterium tuberculosis in clinical specimens as routine laboratory test. So we have compared two DNA isolation methods, 3 polymerases, 2 set of primer, 2 kinds of thermocycler and 2 microtubes. We evaluated two DNA isolation methods, bead-beating method and proteinase-K method, the latter was more sensitive than the former. In comparision of two sets of primers, P1 and P2 primers detecting 123-base pair fragment of IS6110 made from Biosnthesis(U. S. A.) and INS1 and INS2 primers detecting 245-base pair fragment of IS986 showed equally sensitive results ie. 5fg. Specificity of primers were tested and INS1 and INS2 primers gave 245-base pair product from M. tuberculosis H37Rv, M. tuberculosis ATCC 27294 and Mycobacterium bovis ATCC 29312 but not from 8 nontuberculous mycobacterial strains such as M. kansasii ATCC 12478, M. terrae ATCC 15755, M. intracellurae ATCC 13950, M. avium ATCC 25281, M. gordonae ATCC 14470, M. fortuitum ATCC 6841, M. smegmatis ATCC 19420, M. scrofulaceum ATCC 19981. Coagulase-positive staphylococcus, coagulase-negative staphylococcus, streptococcus, Esccherichia coli, Serra-tia marcescens, Klebsiella pneumoniae, Enlerobacter aerogenes, Enterobacter cloacae, Acinetobacter calcoaceticus, Pseudomonas aerugonosa showed no 245-base pair amplification product. PCR by the P1 and P2 primers showed 123-base pair amplification product from M. tuberculosis H37Rv, M. tuberculosis ATCC 27294 and Mycobacterium bovis ATCC 29312 but not from 8 nontuberculous mycobacterial strains such as M. kansasii ATCC 12478, M. terrae ATCC 15755, M. intracellularae ATCC 13950, M. avium ATCC 25281, M. gordonae ATCC 14470, M. fortuitum ATCC 6841, M. smegmatis ATCC 19420, M. scrofulaceum ATCC 19981. Coagulase-postive staphylococcus, coagulase-negative staphylococcus, streptococcus, Escherichia coli, Serratia marcescens, Klebsiella pneumoniae, Enlerobacter aerogenes, Enterobacter cloacae, Acinelobacler calcoaceticus, Pseudomonas aeruginosa showed no 123-base pair amplification product. In comparision of polymerase, Taq DNA polymerase^R(Promega Co., U. S. A.) was more cost effective than Ampli Tag^R(Perkin-Elmer Cetus, U. S. A.), but these two were equally sensitive and specific in the detection of M. tuberculosis-specific DNA. Tac polymerase (Korea'Biotech, Korea) showed many nonspecific bands. In comparision of thermocycler, Easy-Cycler^R(Ericomp, U. S. A.) was more sensitive than water-bath type and in that of microtubes Robbin's were more sensitive than Sarstedt's PCR results were compared with results of culture for M. tuberculosis and Ziehl-Neelsen stain in 370 clinical specimens that were negative by initial Ziehl-Neelsen stain. There were 27 specimens(7.3%) that were positive for M. tuberculosis by culture, 18(4.9%) specimens that were positive by Ziehl-Neelsen stain, and 106 specimens(28.6%) that were positive for PCR and 141 cases that were positive for tuberculosis. Overall sensitivity, specificity, positive predictive value and negative predictive value were 68.8, 96.1, 91.5 and 83.3% respectively, for PCR; 19.1, 100, 100, and 66.8%, respectively for culture; and 12.8, 100, 100, and 59.0% respectively for Ziehl-Neelsen stain.

정보의 재생산과 편집디자인의 관계 : 2007 삼성중공업 Annual Report제작프로세스를 중심으로

서경원 청주대학교 학술연구소 2009 淸大學術論集 Vol.14 No.-

This study examines the process and the procedure through which data information is selected; proposed; and how editorial design is affected based on the agreed upon keyword and content by illustrating the process of designing ‘2007 Samsung Heavy Industries Annual Report.’ This research seeks to suggest the following. Providing a method on how to edit and reproduce information with the given RFP(Request for Proposal;) illustrating the application of the method on whether rational steps were taken; and verifying and sharing the experience of the effects and control drawn by clearing separating editing and editorial design. There can be various methods to obtain complete works of editorial design that everyone can relate to. The effort to illustrate the relational approach of reprocessing information and editorial design was made to suggest one way to secure a complete end product. At times, intuition or a designer’s experience and inclination can create results. However, the probability that such an approach will be effective in successfully producing a publication is low. Therefore, one way to increase the probability of successfully creating a product is to analyze and present procedural processes as this research intends. The degree to which content and design coincide is the issue of the day in editorial design. Design is ultimately a matter of language and visual communication. Precise process can be a rational approach to design. Presenting efficient design through reprocessing information and attractive keyword can play a decisive role in providing clues to visual language. The added contribution of the designer’s intuition and creativity will heighten the probability of communicating rationally and visually with the readers and the clients.

원전 중대사고시 피폭경로 및 핵종의 방사선 피폭에 대한 상대적 중요도 해석

황원태,김은한,김병우,서경석,한문희 대한방사선 방어학회 1994 방사선방어학회지 Vol.19 No.3

원자력발전소의 중대 사고시 대기로 방출된 방사성물질에 의해 피폭자가 사고후 일생동안 받게 될 전신 피폭선량의 핵종의 상대적 중요도를 방출점으로부터 거리에 따라 각 피폭결로에 대해 평가하였다. 방사능운과 지표에 침적된 방사성물지에 의한 외부피폭,호흡과 오염된 음식물섭취에 의한 내부피폭이 피폭경로로 고려되었다. 오염된 음식물섭취에 의한 영향은 우리나라 환경을 고려하여 개발된 동적 섭식경로모델 KORFOOD를 사용하여 침적시점과 침점후 시간에 따른 음식물내 방사성물질의 농도 변화를 고려하였다. 방출점으로부터 80 km까지 피폭선량을 평가한 결과, 오염된 음식물섭취에 의한 영향이 가장 놓았다. 핵종별 기여도는 방사능운에 의한 외부피폭과 호흡에 의한 내부피폭의 경우 I, 침적된 방사성물질에 의한 외부피폭의 경우 Cs에 의한 영향이 가장 높았다. 오염된 음식물섭취에 의한 내부피폭의 경우 Cs은 여름철 침적, Sr은 겨울철 침적에 보다 중요한 영향을 미쳤다. In the case of a severe accident of a nuclear power plant, the whole body does and the relative importance of the radionuclides during the lifetime of an exposed person were estimated for each exposure pathway with distances from the release point. The external exposure pathways due to immersion of radioactive could and deposition of radioactive materials on the ground, and the internal exposure pathways due to inhalation and ingestion of contaminated foodstuffs were estimated considering the variation of radioactive concentration in the foodstuffs according to deposition time and elapsed time after deposition using a dynamic ingestion pathway model applicable to Korean environment, named "KORFOOD". As the results up to 80 km from the release point, the effects due to ingestion of contaminated foodstuffs showed the highest contribution to total exposure does. The contribution of I isotopes was the highest in the case of the external does due to immersion of radioactive cloud and internal does due to inhalation. The contribution of Cs isotopes was highest in the case of the external does due to deposition of radioactive materials on the ground. In the case of the internal does due to ingestion of contaminated foodstuffs, Cs deposition in summer and Sr deposition in winter, respectively, were the most dominant radionuclide to whole body.

반사 시스템 응용에 의한 북측면의 태양 복사에너지 강도

최원기,유경돈,서승직 대한건축학회 2004 대한건축학회논문집 Vol.20 No.11

This paper focuses on applications to a sun reflecting(hereafter referred to as suncatcher) system for the increase of solar radiant energy intensity in the north surface of building. This suncatcher system uses the same principle as reflecting solar collectors and is similar to the 'Le four solaire d'Odello' of French. This paper performs its fundamental study through the simplified numerical model. Preliminary results indicate the suncatcher system could provide profitable conditions for solar thermal collection of the north surface in all climates. As a passive device, application of suncatcher baffle outside the north surface in building could significantly increase the level of thermal collection on a sunny day. Consequently, the suncatcher system is proved to be an effective device for the improvement of environment performance in the north surface. An additional study is required to establish the complete suncatcher system on the basis of this research. This approach could have implications for energy efficiency and conservation, effectively utilizing the free resource of solar radiation.

원자력 사고 중 핵종의 건·습침적에 따른 농작물 오염 영향

황원태,김은한,서경석,한문희,최용호,이창우 대한방사선 방어학회 2002 방사선방어학회지 Vol.27 No.3

핵종의 지표 침적 모델의 고찰과 함께 방사성물질의 공기중 농도로부터 건침적 뿐 아니라 습침적에 따른 농작물 오염 영향을 분석, 고찰하였다. 이를 위해 방사성물질의 지표 침적량으로부터 농작물의 오염을 평가하는 기존 동적 섭식경로모델을 공기중 농도 또는 지표 침적량으로부터 평가할 수 있도록 개선하였다. 평가결과, 방사성물질의 지표 침적량은 습침적에 의한 영향이 건침적에 의한 영향보다 뚜렷이 높으나, 농작물의 오염정도는 핵종, 강우율 등에 따라 다르게 나타났다. 이러한 현상은 방사성물질의 지표 침적과 농작물에로의 차단중 어느 과정이 농작물 오염에 보다 지배적으로 작용하는가에 기인한다. Combined with deposition model onto the ground of radionuclides, the influence of radioactive contamination to agricultural products was analyzed due to wet deposition as well as dry deposition from radioactive air concentration during a nuclear emergency. The previous dynamic food chain model, in which initial input parameter is only radionuclide concentrations on the ground, was improved for the evaluating of radioactive contamination to agricultural products from either radionuclide concentrations in air or radionuclide concentrations on the ground. As the results, in case of deposition onto the ground, wet deposition was more dominant process than dry deposition. While the contamination levels of agricultural products were dependent on the a variety of factors such as radionuclides and rainfall rate. It means that the contamination levels of agricultural products are determined from which is more dominant process between deposition on the ground and interception onto agricultural plants.

모기업 협력업체 산업안전보건관리에 대한 인식

이경용,이관형,오지영,서남규,손두익,갈원모,신문진 한국안전학회 2003 한국안전학회지 Vol.18 No.4

This study is planned to investigate the attitude toward the safety and health management of contractor comparry. Under the contract based production system, all of activities including safety and health management in the contractor company are depended upon the contract. How to make contract influence the worker's health of contractor company. Work's health of contractor company can be protected by efforts of company of contract-out and contractor company, especially their safety managers. The modelling of the effective safety and health management system for contractor company should consider the need of safety manager of each company and employer of contractor company. Data is collected from safety managers of 3 contract out companies as electronic and electrical manufacturing industry and 55 safety managers, 57 employers of their contractor companies using self administered survey with structured questionnaire. The most of all respondents want to support from the contract out companies. The most important items supported from contract out company is the information based on the safety information network between each company. Safety manager and employer of contactor company also itemized safety education and training in the supporting system from contract out company. These results can be generalized to survey on the other industries.

충북 남부지역 중소 제조기업의 경쟁력 제고를 위한 경영 및 애로기술 실태조사 연구

윤상원,구경완,정성진,백기영,김선훈,서용성,이근복 호서대학교 반도체제조장비국산화연구센터 2001 반도체장비학술심포지움 Vol.2001 No.-

본 연구에서는 충북 남부 3개군 지역(옥천 · 보은 · 영동)의 중소 제조기업의 경영실태 및 애로기술을 대상으로 실증조사 되었다.·지역의 특정상황의 전제하에 지방 중소기업들의 실질적인 문제점들을 발견하고, 그 개선 방안을 탐색함은 물론 산 · 학 · 연 컨소시엄 사업의 성공적인 수행을 위해 시도되었다.세부적으로는 중소 제조기업의 경영 및 애로기술 특성을 일반적 사항, 경영애로요인, 경영전략, 현장개선기법, 정보화, 제품 및 공정 기술상의 애로기술 현황파악의 6개 영역으로 조사되었다. 조사결과들은 추후 산 · 학 · 연 공동기술개발 사업에 있어 창의적, 틈새적 과제발굴 및 신제품 기술개발의 토대마련이 될 뿐 아니라, 지역경제 발전을 위한 산 · 학 관의 지원 전략 수립에 적극 활용 될 것으로 판단된다.

HBsAg양성 원발성간암에 있어서 HBV-DNA표지자의 PCR성적

이원길,김재식,김정철,서장수,강은자,이중원,김기연,송경은,양정선 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.2

목적 : HBV-DNA를 정성적 그리고 정량적으로 검출함으로써 강한 전염성과 높은 유병율로 인해 사회적으로 문제가 되는 B형 간염과 원발성간암의 연관성을 알아보고자 하였다. 대상 및 방법 : B형 간염 표면항원(HBsAg)이 양성을 보이는 원발성간암 환자 38명을 대상으로 하였으며 HBV-DNA 중합효소연쇄반응법을 이용하여 정성적 검출 그리고 luminometry법을 이용하여 정량적 검출을 하였다. 결과 : HBV-DNA 중합효소연쇄반응법과 luminometry법의 양성율은 각각 57.9%, 39.5%를 보였다. 그리고 HBsAg과 HBeAg가 동시에 양성을 보인 11명에서는 HBV-DNA 양성율이 81.8%를 보였다. 결론: HBV-DNA 중합효소연쇄반응법의 양성율이 luminometry법에 의한 양성율보다 높은 결과를 보였고 HBV-DNA 검사는 B형 간염을 조기 발견하고 치료하여 만성간질환으로의 진행을 예방하고 특히 원발성간암의 병율을 낮추는데 많이 기여할 것으로 사료되었다. Background : Primary hepatocellular carcinoma(PHCC) is the most common malignant neoplasm in most countries of Asia and Africa. Hepatitis B virus is known to be strongly related to the pathogenesis of PHCC. HBV-DNA PCR and HBV-DNA quantitation assay were attempted to apply to 38 cases of HBsAg positive PHCC. Methods : The ordinary HBV markers(HBsAg, anti-HBs, anti-HBc(Ig-G and -M), HBeAg and anti-HBe) were examined with ELISA. Qualitative screening of HBV-DNA PCR and HBV-DNA quantitation-luminometric measurement were performed. Results : Among 38 cases of HBsAg positive PHCC, positive rates for HBV DNA-PCR and HBV DNA-quantitation were 57.9% and 39.5%, respectively. And 11 cases which were positive for both HBsAg and HBeAg showed 81.8% positive rate in HBV-DNA PCR. Conclusion : For 38 cases of HBsAg positive PHCC, the positive rate for HBV DNA-PCR was higher than HBV-DNA quantitation-luminometry. In HBV-DNA PCR, both HBsAg and HBeAg positive cases showed high positive rate.

유방암종에서 p53, cyclin D1 및 cathersin D 단백발현의 의의

문경래,김환정,이미자,기근홍,서재홍,양정원,조현진 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Background and Objectives: Breast carcinoma is a heterogenous disease. The prognosis of this carcinoma correlates with various prognostic factors. The aim of this study was to assess the prognostic significance of expression of p53, cyclin Dl and cathepsin D protiens in association with various prognostic factors in invasive breast carcinoma. Materials and Methods: The relationship is presented between expression of p53, cyclin D1 and cathepsin D by immunohistochemistry and various established prognostic factors such as tumor size, histologic grade, lymph node status and extensive intraductal component in 55 cases of invasive breast carcinoma. Results: No significant correlations were observed between expression of p53, cyclin Dl and cathepsin D and tumor size. The expression of p53 correlated with high histologic grade. The expression of p53 and cyclin Dl correlated with lymph node metastasis. Conclusion: These results indicate that expression of p53 and cyclin Dl in invasive ductal carcinoma may be an important prognostic factor, closely related to the high histologic grade and lymph node metastasis.