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서장수,이원길,김재식 慶北大學校 醫科大學 1988 慶北醫大誌 Vol.29 No.4
A mixed passive hemagglutintion(MPHA) test using indicator sheep RBC with anti-IgG applied for detection of platelet antibodies in serum. As a result of application, authors have found some merits of MPHA comparing to immunofluorescent method and enzyme immunoassay. 1) The sensitivity and specificity of MPHA are similar to the EIA. 2) The specificity of MPHA is highr than immunofluorescence method. 3) A interpretation of test results is very objective, and a laboratory can be offer semiquantitative antibody titer to physician. 4) The procedure of MPHA is a simple and easy.
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서장수,전효진,김성철,송경은,이원길,김재식 慶北大學校 醫科大學 1989 慶北醫大誌 Vol.30 No.3
To determine the predictability of platelet crossmatching in platelet transfusion, 28MPHA(mixed passive hemagglutination) platelet crossmatchings among 47 plateletpheresis transfusions were tested. Posttransfusion outcome using CCI(correted count increment) were first calculated to detect refractory cases, then crossmatched with calculated samples. The comparison of mean CCI values between the crossmatch-compatible group and the crossmatch-incompatible group showed a statistically significant difference (p<0.005). The overall predictive rates of MPHA crossmatching were 78.6% (1 Hour CCI) and 82.1% (24 Hour CCI). The corrected predictive rates, excluding cases obtaining non-immune factors in causing poor transfusion response, (e.g., splenomegaly, high fever and DIC...) were 86.7% (1 Hour CCI) and 93.3% (24 Hour CCI). These results suggest that the MPHA crossmatching is a useful method in donor selection of plateletphersis, especially, in platelet posttransfusion refractory patients.
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