폐결핵 환자 혈청에서 수용성 Interleukin 2 수용체 및 Adenosine Deaminase 활성도에 관한 연구

고정희,박성규,백상현,박찬권,박병수,안진영,최우석,박정규 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.1

Interleukin-2 (IL-2) induces T cell proliferation in an autocrine manner and provides a means by which antigen triggered T cells can be clonally expanded in vitro. During the following activation, the activity of IL-2 is mediated by specific high affinity IL-2 binding membrane receptors which are expressed shortly after activation. In this process, a 42 KD-fragment (soluble IL-2R) is continuously cleaved off and circulates as a soluble marker of T lymphocyte activation. Elevated level of soluble IL-2R has been identified in the serum of patients with malignant autoimmune and allergic disorders, systemic parasitic infection, undergoing graft versus host disease, acute or chronic lymphocytic leukemia and HIV-infection. ADA (adenosine deaminase) completes the process of differentiation of T cell and is essential for progression of T cell maturation. Therefore level of ADA is to correlated with magnitude of T cell immune response. The fact that expression of sIL-2R and ADA activity increases in peripheral blood T-lymphocytes of patients with active pulmonary tuberculosis suggests that T cell activation might have a major role in the pathogenesis of pulmonary tuberculosis. In order to evaluate the T cell immune response in pulmonary tuberculosis, we measured the serum concentration of sIL-2R and ADA activity in 17 patients with current pulmonary tuberculosis, 10 chronic inactive pulmonary tuberculosis, 10 as normal controls. (1) Current pulmonary tuberculosis had significantly higher levels of sIL-2R (237.24±95.47)when compared with those of inactive tuberculosis (78.6±11.06). and the control (68.17±15.4) group. (2) ADA activity in current pulmonary tuberculosis was significantly increased (34.41±20.63) when compared with those of inactive tuberculosis (24.7±14.36) and control (17.65± 5.94) group. (3) There was good correlation between sIL-2R concentration and ADA activity in serum in current pulmonary tuberculosis group. (4) sIL-2R concentration and ADA activity was decreased significantly 6 months after anti-tuberculosis drug medication. In conclusion, sIL-2R concentration and ADA activity in serum in current pulmonary tuberculosis group was increased when compared with those of inactive pulmonary tuberculosis and the control group. By the way, T cell mediated immune response was enhanced in current pulmonary tuberculosis, but in inactive chronic pulmonary tuberculosis who had treated by antituberculosis drug medication, the concentration of sIL-2R and ADA activity was nearly normal.

대량의 각혈로 사망한 장티푸스 1예

한상우,유진홍,권순석,김성훈,박지찬,홍은정,최경성,박용완,장준희,안지원,박유경,강지영,박상미,신완식 대한감염학회 2004 감염과 화학요법 Vol.36 No.5

We experienced a 25 year-old male patient with typhoid fever complicated with massive hemoptysis. Pulmonary complication in typhoid fever is very rare and to our knowledge, there has been no report of hemoptysis as a main cause of death with this disease. We herein report a rare case of typhoid fever.

Influence of Sowing Time on Growth, Yield and Nutritional Quality of Forage Rape in Spring

Kwon,Byung-Sun,Shin,Jeong-Sik,Park,Hee-Jin,Shin,Jong-Sup,Choi,Seong-Yu 한국자원식물학회 2003 Plant Resources Vol.6 No.1

To determine as optimal sowing time of forage rape in spring time in southern areas of Korea, forage rape cv. Velox, the highest yielding variety among introduced varieties of forage rape, was grown under five different sowing times. Yield components such as plant length, number of branches and number of leaves etc. were higher at the sowing time of Mar. 15 and Mar. 25. The plants sown at Mar. 15 and Mar. 25 also showed highest fresh and dry matter yield. When plants were grown under later sowing time, they showed higher values in content of crude protein and lower values in contents of crude fiber such as Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), cellulose and lignin. There was no relationship between variation of In Vitro Dry matter Digestibility (IVDMD) and sowing time. The plants sown at Mar. 15 and Mar. 25 showed highest digestible dry matter yields.

분산 데이타베이스 설계를 위한 데이터 분할 방법론

박성진,백두권 한국경영과학회 1994 한국경영과학회 학술대회논문집 Vol.- No.2

최근들어 분산 데이터베이스 시스템에 관한 연구가 연구 목적뿐만 아니라 실질적인 응용 분야에서도 급속히 증가하고 있다. 한편, 분산 데이터베이스 시스템에서 데이터 분할은 주요한 설계 이슈중의 하나이다. 비록 데이터 단편화 설계에 관한 많은 연구가 진행되었지만 기존 연구의 대부분은 일시적인 해결책이나 특별한 경우에 대한 일반적인 해결책에 그치고 있으며 단순히 수평 혹은 수직 분할 방법론만을 선택적으로 적용하고 있다. 본 논문에서는 혼합 단편화에 기반한 분할 방법론을 제안하였다. 제안한 방법론은 후보 단편들을 생성하고 데이터 할당과 중복을 위한 최적의 단위를 제공한다.

mRNA의 3' end processing에 대한 mRNA 중합요소 인산화의 영향

권대환,박재현,송영하,안성훈 한양대학교 이학기술연구소 2006 이학기술논문지 Vol.9 No.-

RNA 중합효소의 C-terminal은 YSPTSPS의 반복적인 아미노산 서열을 갖고 있으며, 이들의 인산화는 전사의 개시, 중합, 또는 종결단계에 중요하다고 알려져 있다. 본 연구에서는 이러한 RNA 중합효소의 인산화가 mRNA의 3' end processing에 미치는 영향을 조사하였다. C-terminal domain (CTD) of RNA polymerase Ⅱ contains a repeated heptameric sequence (YSPTSPS) which is phosphorylated at serine 2 and 5. Phosphorylation of CTD in elongating RNA polymerase Ⅱ has been suggested to play a role in transcriptional initiation, elongation or termination. In the present study, we investigated the effect of RNA polymerase Ⅱ CTD phosphorylation on the 3' end processing of mRNA.

전주,익산지역 산업단지 악취 및 유해대기오염물질 발생원 조사

권영호,박성순,양고수 전북대학교 공학연구원 ( 구 전북대학교 공업기술연구소 ) 2002 工學硏究 Vol.33 No.-

VOCs (Volatile Organic Compounds) have been produced commercially and used for many purposes in the chemical industry including the manufacture of herbicides, plastics, and solvent. Their emissions affect the change of climate, the growth and decay of plants, and the health of human beings and animals. Therefore it is necessary to limit and control of VOC emission. There are various removal technologies of VOCs, such as catalystic oxidation, biofiltration, Adsorption, Thermal oxidation and Menbrane separation. In this study, we examined both source and components of odor and VOC pollutants emitted from Jeonju and Iksan industrial Area. According to the results, the main VOCs used in the two industrial Area were benzene, toluene, chloroform, methanol, methylene chloride, xylene, ethyl ethyl ketone etc. Especially, hydrogen peroxide and methanol were extremely used in these complex. The regulation of VOC will be more strict within near future. Therefore, this research work will be applicable removal efficiency of VOC emitted from Jeonju and Iksan industrial Area as a fundamental data.

AOPWIN을 이용한 Acetanilide의 광분해 특성 평가

권민정,최윤호,송상환,박혜연,구현주,정성환,나진균,박광식 한국환경독성학회 2001 환경독성보건학회지 Vol.16 No.3

Acetanilide is a High Production Volume Chemical, which is produced about 2,300 tons/year in Korea as of 1998 survey. Most is used as an intermediate for synthesis of pharmaceuticals and dyes, and the chemical is one of seven chemicals of which human and environmental risk are being assessed by National Institute of Environmental Research under the frame of OECD SIDS program. The Atmospheric Oxidation Program for Microsoft Windows (AOPWIN) is used to estimates the rate constant for the atmospheric, gas-phase reaction between photochemically produced hydroxyl radicals and organic chemicals. It is also used to estimates the rate constant for the gas-phase reaction between ozone and olefinic/acetylenic compounds. The rate constants estimated by the program are then used to calculate atmospheric half-lives for organic compounds based upon average atmospheric concentrations of hydroxyl radicals and ozone. AOPWIN requires only a chemical structure to make these predictions. Structures are entered into AOPWIN by SMILES (Simplified Molecular Input Line Entry System) notations. In this study, one of environmental fate/distribution of the chemical elements, photodegradation of acetanilide was estimated using AOPWIN model based on SMILES notation and chemical name data.

스테이지 이동 반력에 의한 베이스 진동 저감기법 개발

박아영,김형기,권오영,홍성욱 한국공작기계학회 2009 한국공작기계학회 춘계학술대회논문집 Vol.2009 No.-

A variety of precise stages are adopted for precise machinery. To secure vibration isolation, those precise stages are mounted on a heavy base structure supported by compliant springs. Then the base structure is often subjected to vibration due to the reaction force induced by the stage motion. This paper investigates the dynamic behavior of base structures as well as a vibration reduction technique. A 6 DOF dynamic model is established to investigate the vibration of positioning stage and base structure. A new input shaper is devised to eliminate both the residual vibrations in base structure. Simulations show that the proposed input shaper effectively remove the residual vibrations in base structure.

연 X-선 현미경을 이용한 금 나노입자 세포영상

권영만,김한경,김경우,김선희,윤홍화,천권수,강성훈,박성훈,정선관,윤권하 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.3

연 x-선 현미경은 ‘물의 창’ 영역(2.3~4.4 nm)의 파장을 이용하여, 수십 nm의 분해능으로 세포를 파괴하지 않고 살아있는 상태에서 세포의 내부구조를 관찰할 수 있어 가시광선현미경과 전자현미경을 단점을 보완하는 특징을 갖는 세포 생물학 연구에 적합한 현미경이다. 그러나 기존 연 x-선 현미경은 광원으로 방사선 가속기를 이용하기 때문에 사용이 제한적이었다. 이에, 본 연구에서는 2.88 nm의 연 x-선을 광원으로 사용하는 소형 연 x-선 현미경을 이용하여, 내포작용에 의해 금 나노입자를 포획한 HT1080과 MDA-MB 231 세포의 영상을 약 60 nm 분해능으로 획득하였다. 금 나노입자의 세포에 대한 독성을 제거하기 위하여 폴리에틸렌 글리콜을 캡핑하였고, 2.88 nm 파장의 연 x-선에 대하여 충분한 조영효과로 인하여 세포영상에서 뚜렷한 대조도를 나타내었다. 내포작용에 의해 액포에 포함되어 있는 다양한 크기의 금 나노입자 군집을 확인하였으며, 세포내부의 액포의 분포상태도 관찰할 수 있었다. 따라서 고분해능을 가진 소형 연 x-선 현미경을 이용하여 금 나노입자를 세포내의 미세기관이나 특정 단백질에 표지하면 연 x-선에 대한 조영효과의 증가에 의하여 더욱 유용한 정보를 획득할 수 있을 것으로 생각한다. A compact soft x-ray microscope operated in the ‘water window’ wavelength region (2.3~4.4 nm) was used for observing cells with nano-scale spatial resolution. To obtain cellular imaging captured with colloidal gold nanoparticles using a compact soft x-ray microscope. The colloidal gold nanoparticles showed higher contrast and lower transmission more than 7 times than that of cellular protein on the soft x-ray wavelength region. The structure and thickness of the cell membrane of the Coscinodiscus oculoides (diatome) and red blood cells were seen clearly. The gold nanoparticles within the HT1080 and MDA-MB 231 cells were seen clearly on the soft x-ray microscopy. The gold nanoparticles were aggregated within vesicles by endocytosis.

두경부에서 생긴 림프종에서 EBV와 HTLV-1의 검색

박준식,정효순,권성우,이상숙 경북대학교 병원 2003 경북대학교병원의학연구소논문집 Vol.7 No.1

Background and Objectives : Extranodal non-Hodgkin's lymphoma(NHL) of the head and neck(H&N) accounts for 10-20% of all cases of NHL. Despite their frequency, the cause of these lymphomas is still poorly understood. Recently, the role of viral origin in NHLs, including Epstein-Barr virus(EBV), as the main cause of sinonasal lymphomas of T/NK cell phenotype and HTLV-1 as a cause of acute T-cell lymphoma/leukemia has been well documented. We investigated the clinicopathologic findings, immunophenotypic profile, and status of EBV and HTLV-1 DNA of patients with H&N lymphoma. Materials and Methods : Twenty-seven patients with NHL of H&N region were studied. There were 15 males and 12 females with the median age of 50 years. All patients were reclassified according to the Working formulation(WF) and REAL classification. EBV genome DNA and HTLV-1 RNA were surveyed by PCR assay using formalin-fixed and paraffin-embedded tissue blocks. Results : The tonsil was the most commonly involved site(44.4%) followed by nasal cavity(18.5%), nasopharynx(18.5%) and orbit(7.4%). Immunophenotyping revealed 19 cases of B cell lineage, 7 cases of T cell lineage and one case of null cell type. Most of B-cell lymphomas were diffuse large cell lymphomas(58%). Tonsillar lymphomas were all B-cell origin. Four of the five nasal cavity lymphomas and one nasopharyngeal lymphoma showed an angiocentric T/NK cell phenotype with strong association with EBV. EBV genome was detected in 15 of 26 H&N NHLs(57.7%). Seven of 19 B-cell lymphomas(36.8%) and all T/NK or null cell type lymphomas were positive for EBV DNA. However, there was no HTLV-1 positive cases found. Conclusions : It could be concluded that the high incidence of EBV of angiocentric T/NK-cell lymphomas of the nasal cavity may indicate a probable role of EBV in the development of these lymphomas.