-
RISS 활용도 분석
-
일본/중국/유럽자료
-
최신/인기 학술자료
-
해외논문구매대행(E-DDS)
http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 박광식 (검색결과 255 건) 검색분석정보 보기
- 무료
- 기관 내 무료
- 유료
-
-
-
-
-
박광식,George Tuttle,Federico Sinche,Stacey L. Harper 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.1
The stability of citrate-capped silver nanoparticles(AgNPs) and the embryonic developmental toxicitywere evaluated in the fish test water. Serious aggregation ofAgNPs was observed in undiluted fish water (DM-100) inwhich high concentration of ionic salts exist. However,AgNPs were found to be stable for 7 days in DM-10,prepared by diluting the original fish water (DM-100) withdeionized water to 10 %. The normal physiology of zebrafishembryos were evaluated in DM-10 to see if DM-10can be used as a control vehicle for the embryonic fishtoxicity test. As results, DM-10 without AgNPs did notinduce any significant adverse effects on embryonicdevelopment of zebrafish determined by mortality, hatching,malformations and heart rate. When embryonic toxicityof AgNPs was tested in both DM-10 and in DM-100,AgNPs showed higher toxicity in DM-10 than in DM-100. This means that the big-sized aggregates of AgNPs werelow toxic compared to the nano-sized AgNPs. AgNPsinduced delayed hatching, decreased heart rate, pericardialedema, and embryo death. Accumulation of AgNPs in theembryo bodies was also observed. Based on this study,citrate-capped AgNPs are not aggregated in DM-10 and itcan be used as a control vehicle in the toxicity test of fishembryonic development.
-
인간 폐포세포 유래 A549세포주에서의 Cadmium 처리에 의한 메탈로치오닌 유전자 발현
박광식,구자민 한국환경독성학회 2003 환경독성보건학회지 Vol.18 No.1
Metallothionein gene expression activity of cadmium was investigated in a human lung epithelial cell line. Cells, grown to near confluence, were exposed to 0~10 μM Cd metal for 6 hours. Cadmium did not cause morphological alteration in lung epithelial cells that are characteristic of cell damages such as cell shrinkage, detachment of the cell from its neighbors, cytoplasmic and chromatic condensation. However, metallothionein genes of MT-I and MT-2 were rapidly induced in the treated cell measured by RT-PCR. Regarding the induction pattern of metallothionein mRNA, MT- I mRNA was induced in a dependent manner. MT~2 mRNA induction, which was measured using oligo primers based on cDNA of human reticulocytes, seemed to be slightly increased in low doses but decreased at high concentration used in the experiment.
-
-
-
-
