Choline dehydrogenase interacts with SQSTM1/p62 to recruit LC3 and stimulate mitophagy.

Park, Sungwoo,Choi, Seon-Guk,Yoo, Seung-Min,Son, Jin H,Jung, Yong-Keun Landes Bioscience 2014 AUTOPHAGY Vol.10 No.11

<P>CHDH (choline dehydrogenase) is an enzyme catalyzing the dehydrogenation of choline to betaine aldehyde in mitochondria. Apart from this well-known activity, we report here a pivotal role of CHDH in mitophagy. Knockdown of CHDH expression impairs CCCP-induced mitophagy and PARK2/parkin-mediated clearance of mitochondria in mammalian cells, including HeLa cells and SN4741 dopaminergic neuronal cells. Conversely, overexpression of CHDH accelerates PARK2-mediated mitophagy. CHDH is found on both the outer and inner membranes of mitochondria in resting cells. Interestingly, upon induction of mitophagy, CHDH accumulates on the outer membrane in a mitochondrial potential-dependent manner. We found that CHDH is not a substrate of PARK2 but interacts with SQSTM1 independently of PARK2 to recruit SQSTM1 into depolarized mitochondria. The FB1 domain of CHDH is exposed to the cytosol and is required for the interaction with SQSTM1, and overexpression of the FB1 domain only in cytosol reduces CCCP-induced mitochondrial degradation via competitive interaction with SQSTM1. In addition, CHDH, but not the CHDH FB1 deletion mutant, forms a ternary protein complex with SQSTM1 and MAP1LC3 (LC3), leading to loading of LC3 onto the damaged mitochondria via SQSTM1. Further, CHDH is crucial to the mitophagy induced by MPP+ in SN4741 cells. Overall, our results suggest that CHDH is required for PARK2-mediated mitophagy for the recruitment of SQSTM1 and LC3 onto the mitochondria for cargo recognition.</P>

제3분과 : 야생동물 ; 국립공원 산불통제구간내 야생동물 서식현황 분석 -지리산국립공원 노고단~피아골계곡을 중심으로-

강병선 ( Byung Seon Kahng ),박기연 ( Ki Yeon Park ),김은창 ( Eun Chang Kim ),이재윤 ( Jae Yoon Lee ),지기선 ( Ki Seon Ji ),최형진 ( Hyung Jin Choi ),오현경 ( Hyun Kyung Oh ) 한국환경생태학회 2013 한국환경생태학회 학술대회지 Vol.2013 No.1

우리나라 전 국토의 6.58%를 차지하고 있는 국립공원은 생태계 현황을 대표하는 지역으로 한국에 서식하는 생물종 39,150종 중 40%에 해당하는 15,727종이 서식하고 있다(국립공원관리공단, 2012). 특히 환경부에서 지정한 멸종위기종 221종 중에서 65%에 해당하는 144종이 서식하고 있어 인간의 간섭 및 개발압력으로부터 마지막 피난처의 중요한 역할 또한 수행하고 있다. 국립공원은 자연생태계와 자연 및 문화경관 등을 보전하고 지속가능한 이용을 도모함을 목적으로 하는 자연공원법에 의하여 지정되는 자연공원의 하나로서 자연생태계의 보전뿐만 아니라 지속가능한 이용 또한 고려되어야 한다. 즉, 생물종 서식처의 보존 뿐만 아니라 탐방객의 이용 또한 고려되어야 하며 이를 위해서는 생물종의 서식현황 분석은 반드시 선행되어야 할 과제이다. 그러나 생물종이 다양하고 서식처가 광범위하여 종의 전체적인 서식현황을 분석에는 많은 시간과 비용이 수반된다. 따라서 시간과 공간이 제한되는 산불조심기간 중 산불통제 구간을 대상으로 야생동물의 서식현황을 분석해 보고자 하였다. 아울러 구례군 지역에 서식하는 야생동물의 서식현황을 분석하여 보호지역 관리에 기초자료를 제공하고자 하였다. 월 1회 정기적으로 탐방로를 중심으로 야생동물의 식흔, 배설물 등 흔적을 2010년부터 2012년까지 최근 3년간 모니터링 하였으며, 모니터링 시 GPS 좌표 측점, 사진촬영, 주변환경조사 등을 병행하였다. 아울러 무인센서카메라를 10대 설치하여 실체를 확인하였으며, 조사된 자료는 GIS DB로 최근 3년간, 지리산국립공원 노고단~피아골계곡에서 조사된 야생동물 흔적은 124건으로 삵 59건, 담비 27건, 족제비 21건, 멧토끼 10건, 멧돼지 4건, 고라니 2건, 오소리 1건 등이었다. 이중 삵과 담비는 멸종위기종으로서 86건으로 발견종의 69%를 차지하고 있었다. 특히 봄과 가을철 산불통 제기간 중에는 월평균 19회 관찰되었는데 이는 다른 기간 평균 4회 보다 5배정도 많은 횟수로 탐방객의 출입이 제한되었을 때 위협요인이 감소됨으로서 서식여건이 보다 안정화되어 훨씬 많이 발견되고 있음을 알 수 있었다. 2010년부터 2012년 지리산국립공원 구례지역에서 조사된 야생동물 698건의 서식 분포특성을 분석한 결과 삵 286건, 담비 139건, 족제비 95건, 수달 53건, 오소리 30건, 멧돼지 30건, 멧토끼 29건, 너구리 16건, 고라니 13건, 노루 4건, 하늘다람쥐 3건 등이었다. 특히 멸종위기종인 삵, 담비, 수달, 하늘다람쥐 등이 481건으로 68%를 차지하고 있어 멸종위기 야생동물의 출현빈도가 매우 높음을 알 수 있었다. 최근 3년간 야생동물 서식현황을 분석한 결과 타기간 보다 산불방지기간에 출현빈도가 높아 탐방객의 출입에 따른 위협요인을 계량적으로 파악할 수 있었으며, 특히, 봄철 산불방지기간은 야생동물의 번식기라는 점에서 보다 안정된 서식처를 제공한다는 의의가 있을 수 있다. 또한 멸종위기종의 흔적이 전체종의 68%가 발견됨으로서 멸종위기종의 서식처를 제공하고 있음을 알 수 있었다. 향후 이러한 결과를 바탕으로 각 야생동물에 대한 서식처 분석과 더불어 이를 통한 관리방안이 수립될 필요성이 있다.

Variation of Bird community and Habitat use after Developing Hangang river Ecological Learning Center

Paik, In-Hwan,Jin, Seon-Deok,Jeon, Byung-Seon,Yu, Jae-Pyoung,Park, Chi-Young,Paek, Woon-Kee national science museum of korea 2010 Journal of Korean nature Vol.3 No.4

The Hangang (Han River) Ecological Learning Center is a river ecosystem park which was completed in December of 2002. The river ecosystem park was created based on the understanding of the problems of standard park-formed river which centers around convenient facilities instead of on the wish to accentuate the innate unique characteristics of a river. Therefore, the study was conducted in order to analyze the changes and habitation usage of the avifauna in the Hangang Ecological Learning Center to provide basic information for the improvement of bird species diversity in an ecosystem park. The study was conducted in July and October of both 2003 and 2005 to study the avian colonies of the same time period, and the habitat usage of four different areas were examined. Main dominant species includes species which preferred waterside and grassland, and 2005 showed higher levels of species, bird count and number of migratory birds. Furthermore, among the four regions used as habitats, the confluence region showed the highest level of increase, and this is predicted to have caused by the shallow depth of the confluence and the stability of the waterside plant colonies.

어린이 공공전문진료센터의 진료 역량: 상급종합병원과의 비교를 중심으로

오정윤,조수진,정진선,조진숙,박춘선 건강보험심사평가원 2022 HIRA RESEARCH Vol.2 No.2

소아청소년 복합만성질환자 수 및 진료비의 변화: 2011-2021년 건강보험청구자료 분석

오정윤,조수진,정진선,조진숙,박춘선 건강보험심사평가원 2023 HIRA RESEARCH Vol.3 No.2

Background: The number of children and adolescents has decreased by more than 3 million over the past 10 years. We analyzed changes in the number of children and adolescents with complex chronic conditions (CCCs) and their medical expenditures between 2011 and 2021. Methods: We used National Health Insurance claims data of patients under the age of 20 from 2011 to 2021. CCCs were classified as pediatric CCCs by Feudtner et al. Patients who repeatedly utilize medical services due to CCCs were defined as patients who sought medical care twice or more within the past 3 years. Their sex, age, CCC categories, and the number of CCC categories were presented using descriptive analyses. Results: The number of children and adolescents with CCCs increased from 345,320 (3.04%) in 2011 to 451,238 (5.33%) in 2021. Patients who repeatedly utilized medical services due to CCCs increased by 71,889 during the same period. The proportion of patients with two or more CCC categories increased from 35.80% in 2011 to 43.67% in 2021. In 2021, patients with other congenital or genetic defect were most common. Medical expenditures associated with CCCs increased from 475,654 million won (12.34%) in 2011 to 768,467 million won (19.11%) in 2021. Conclusion: The number of patients with CCCs and their medical expenditures increased in Korea between 2011 and 2021. The results indicate that consistent national efforts are required to maintain the specialized pediatric care. Further studies are needed to identify pediatric patients who require specialized medical services through various methods.

한국 정신장애의 역학 조사 연구[I] : 각 정신장애의 유병률

조맹제,함봉진,김장규,박강규,정은기,서동우,김선욱,조성진,이준영,홍진표,최용성,박종익,이동우,이기철,배재남,신정호,정인원,박종한,배안,이충경 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.4

Objectives : This study aims to estimate the prevalence of the DSM-IV psychiatric disorders in Korean population using the Korean version of Composite International Diagnostic Interview (K-CIDI). Methods : Subjects were selected by taking multi-stage, cluster samples of 7,867 adult household residents, 18 to 64 years of age, in ten catchment areas. Total 78 trained interviewers administered the K-CIDI to the selected respondents, from June 1 to November30,2001. Results : Total 6,275 respondents completed the interview. Some 33.5% of respondents reported at least one lifetime disorder, 20.6% reported at least one-year disorder, and 16.7% reported at least one-month disorder. The most common lifetime disorders were alcohol abuse/dependence (17.24%), nicotine dependence/withdrawal (11.19%), specific phobia (5.16%), and major de-pressive disorder (4.25%). The lifetime prevalence of substance abuse/dependence (0.25%) and schizophrenia (0.16%) was very low. Nicotine and alcohol use disorder showed very high male/female ratio. Mood disorder and anxiety disorder were more prevalent among female than male. Conclusion : The prevalence of psychiatric disorders was high. In comparison with other studies, remarkable differences in distributions of psychiatric disorders across the areas and times were observed.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

노인의 Helicobacter pylori 재균율

박주상,홍원선,이영수,이은주,명승재,정훈용,양석균,김진호,민영일 대한노인병학회 2000 Annals of geriatric medicine and research Vol.4 No.3

"한국판 학령전기 기질성격검사[K-psTCI]" 의 신뢰도 및 타당도 검사

박진균,김지웅,기선완,신의진 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.4

Objectives : The purpose of this study was to evaluate the reliability and validity of the Korean version of Preschool Temperament and Character Inventory (K-psTCI), a questionnaire based on Cloninger's seven-factor model of personality. Methods : The psTCI was translated into Korean and administered to 266 children aged 2-6 years. α test-retest study of the K-psTCI was conducted across a 4-month interval, Internal consistency was calculated by Cronbach a. Test-retest and reliability was analyzed by Pearson correlation analysis. Factor analyses for the temperament and character dimensions were performed using principal component analysis, rotating factors by varimax. The psTCI scores of Korean and United States preschoolers were compared Results : Cronbach α values for the K-psTCI scales ranged from .62 to .78 for each dimensions. Test-retest correlations (r) ranged from .50 to .77 for each dimensions. Explorative factor analysis with the condition of eigenvalue greater than 2 produced four factors for the temperament items and three factors for the character items like the US original version of psTCI. Through factor analyses, five items in the K-psTCI were categorized differently from the US version of psTCI. Korean preschoolers had higher mean scores on Cooperativeness (CO) in males as compared to a sample of US preschoolers. Conclusion : The results of this study suggests that the Korean preschool TCI is satisfactory in reliability and validity.

암면재배 수박의 품종별 당함량 비교

박순기,이범선,최진호,김월수,정순주 전남대학교 농업과학기술연구소 2000 農業科學技術硏究 Vol.35 No.-

The fruit qualities and sugar contents of hydroponically grown watermelon cultivars were investigated in order to obtain informations for nutriculture of watermelon in glass house. The fruit circumference of Mudeungsan watermelon was increased from 30 to 38 days after pollination, and periods of fruit development was longer than that of other cultivars. Total soluble solid content in fruit flesh of watermelon was high in Samboggool, Gwigongja, Dalgona, Gamro, Mudeungsan, Olimpia, Hwangto and Geumro in order. Maltose content was higher in Gwigongja and Mudeungsan watermelon cultivar Sucrose content was higher in Samboggool, Gwigongja and Dalgona watermelon cultivar than that of other watermelon cultivars. Glucose content was much higher in Mudeungsan watermelon cultivar while very lower in Samboggool, Gwigongja and Dalgona watermelon cultivar than that of other watermelon cultivars. Fructose content was higher in Hwangto, Gamro, and Mudeungsan watermelon cultivar while was lower in Gwigongja watermelon cultivar than that of other watermelon cultivars. Maltose, glucose, and fructose content in fruit fresh of watermelon was higher in Mudeungsan watermelon than that of other watermelon cultivars. Contents of maltose, fructose and glucose in fruit flesh of watermelon were higher in Mudeungsan watermelon than those of other cultivars.