Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

반공포로의 석방과 국민형성의 딜레마

최미진(Choi, Mi-Jin) 부산대학교 한국민족문화연구소 2011 한국민족문화 Vol.41 No.-

이 글의 목적은 김광주의 『석방인』을 대상으로 휴전 전후 반공포로 석방의 의미항을 고찰하는 데 있다. 휴전 직전 이루어진 반공포로 석방사건을 재현한 이 소설은 전후사회의 상황을 후경으로 삼아 반공포로들이 남한사 회의 ‘국민’으로 자리매김하는 문제를 집중적으로 다룬다. 우선, 이 소설은 부산 가야리 제9수용소를 배경으로 반공포로의 석방과정을 사실적이면서도 극적으로 재현한다. 가장 기동적인 신문매체를 통해 보도되었던 당시의 사실을 재현하고 문맥화하는 한편 극적 재구성을 통해 실감을 확보하고 있었다. 특히 반공포로의 자발성과 국가의 주도적 역할에 대한 강조는 휴전 직전 이승만 정권의 독자적 행보를 정당화하는 국가이데올로기의 순응 장치로서 기능하고 있었다. 그리고, 석방된 반공포로 박철은 고백 행위를 통해 남한 사회에서 자신의 정체성을 확보하고 사회적 위상을 새롭게 하는 계기를 마련하고 있었다. 다르게 말하면 남한 사회가 요구하는 사상증명으로서 박철의 고백은 일종의 과거 청산인 동시에 현재의 비참한 상황을 극복할 수 있는 자기 구원에의 의지를 지닌 것이었다. 그럼에도 고백 행위가 이루어지는 교차적 회상구조는 반공포로를 ‘보호’의 대상이자 ‘갱생’의 대상으로 간주했던 당대 상황을 반영한다. 즉 남한사회가 반공포로에 대해 동포애주의와 사상증명사이에서 부유하던 국민형성의 딜레마를 재현하고 있었다. 마지막으로, 이 소설은 반공포로의 ‘석방’을 과거청산에서 과거극복으로 재의미화하여 국민형성의 딜레마를 지양하고자 하였다. 문화인에 대한 작가의 남다른 애정과 전후사회 재편의 새로운 방향을 보여주는 시각이었다. 특히 석방관 건립은 한국전쟁의 상처를 기억하고 그러한 체험을 가진 이들을 애도한 공적 문화사업의 일종으로, 박철을 비롯한 연극인, 나아가 남한사회의 모든 국민들이 반공이데올로기에 편향되어 경직된 과거청산에 함몰되기 보다는 건설적인 미래를 위한 전후복구의 청사진을 제시하고 있었다. 그것은 주체적 여성 최미자를 통해 이루어지고 있으나, 주체적 여성의 독자적인 부상이라기보다 전후사회를 이끌 새로운 주체의 요건을 제시하는 것에 가까웠다. 이러한 결과를 두고 볼 때, 『석방인』은 ‘제시하는 것’에 침묵하는 세태소설이 아닌 세태를 읽어내는 작가의 시선이 건강하게 살아있는 소설이라 할 수 있다. This study aims at exploring the meaning of the release of anticommunist POWs before and after the Korean War armistice, by looking into Seokbangin (The Released) written by Kim, Gwang-Ju. This novel, which describes the incident of setting anticommunist prisoners of war free just before the Korean war armistice, focuses on the issue of helping the released POWs to put down roots in the South Korean society during the post-war period. First, set against the 9th prison camp in Gaya-ri, Busan, this novel reproduces the process of discharging anticommunist prisoners of war in a very realistic and dramatic way. Some communicable facts are reenacted through the medium of newspapers and put into context, while dramatic reconstruction increases its realism. In particular, the emphasis on the voluntary acts of anticommunist prisoners of war and on the dominating role of the nation serve as a device conforming to the national ideology of the time justifying the unilateral conducts by the Lee, Seung-Man Administration right before the armistice. As Park Cheol, the discharged anticommunist prisoner of war, makes a confession, he tries to renew his identity in South Korea and find an opportunity to rebuild his social status. In other words, he attests to the ideology demanded by the South Korean society. His confession represents both his efforts for the liquidation of the past and his willingness to rescue himself from the miserable situation. In addition, the structure of crossing recollections used for his confession suggests the dilemma of the time about embracing the released anticommunist POWs as South Korean people, indicating not only the state of mind of the released POWs who had to choose either brotherly love or ideology attestation but also the conflicts facing the South Korean society at the time. Lastly, this novel re-signifies the "release" of the anticommunist prisoners as an act of overcoming the past, rather than the liquidation of the past, rejecting the dilemma about embracing them into the nation. This clearly shows the extraordinary affection toward the cultured people and a new direction for the post-war society. As part of public cultural projects aimed at remembering the wounds of the Korean War and paying tribute to all those who underwent the terrible war, the establishment of the "Seokbanggwan (Release Memorial)" shows that Park Cheol and other theatrical people and all South Korean people, in a broader context, did not confine themselves to the liquidation of the past based on the anticommunist ideology, but presented a new blueprint for post-war reconstruction work to usher in a brighter future. Even though these works have been mostly done by Choi, Mi-Ja, an independent lady, it was more like suggesting the qualifications for a new player who can lead the post-war society, than the emergence of independent women. This female character was nothing but the embodiment of the men"s perspective. Given all these study outcomes, Seokbangin is not a novel of manners, which remains quiet about the current situation, but it can be rightly called a novel where the writer"s point of view on the society is clearly apparent.

한국전쟁기 김말봉의 『별들의 故鄕』 연구

최미진(Choi Mi­Jin),김정자(Kim Jeong­Ja) 한국문학회 2005 韓國文學論叢 Vol.39 No.-

This paper takes a close look at Kim, Mal-bong's Hometown of Stars released during the Korean War period, in order to relate the writer's literary characteristics seen after the liberation from the Japanese colonial rule to the characteristics in social melodrama. Hometown of Stars adopts the formula of social melodrama that gives its unique double effect by combining basic plot of love stories among characters with social and historical events as a background. First, in the Hometown of Stars, the Licensed Prostitution Abolition Movement after the Liberalization plays a role in diversifying love relationship among characters and gives an opportunity for self-reflection. In particular, the Movement is used to highlight the changing social views of the characters. The writer indirectly sheds light on the inhumane treatment of prostitutes and the problems of residual values of the feudalistic way of thinking based on the class system. However, his intention to put the behind story of social and historical facts into a realistic context, rather than to investigate the Movement in itself helps intensify the pleasure to read this novel. Next, the social and historical background of the Korean War serves as a vehicle to provide plausibility to the plot and to raise readers' awareness of the reality through the hidden fact of history. The writer's views that the government's misinformation at the time of the Korean War helped increase people's casualties are represented in a natural fashion through characters' behaviors, evoking a sense of reality on the historical facts from readers. Moreover, the War, which serves as a defining moment in dramatizing love conflicts of the characters, boosts the interest in reading the story. The writer sets the Korean War as a turning point to convert the ideological confrontation after the Liberalization to the punitive confrontation structure in which virtue is rewarded and vice is punished. The ending of Hometown of Stars shows both melodramatic limit and social melodramatic characteristics that go beyond the limit, because the clash between the prevailing ideology and the moral faith forms the typical punitive confrontation structure, while transcending the formula to reach the universal value of humanity, especially religious ethics. The writer is not swayed by conventions or ideology and he uses “star” as a metaphor of human and spirit, hoping that they can be reborn in the “hometown”, the source of new hope. In this symbolic fashion, he suggests a “wise” way to overcome wartime confusion to readers. The formula of social melodrama in Hometown of Stars not only provides pleasure of reading popular novels, but also puts social and historical facts into a realistic context and deals a measurable blow to covered historical facts and ignored social thinking frame. In that sense, Kim, Mal-bong could succeed in strengthening literature's impact by the means of social melodrama that contains popular and social elements at the same time. This is related to his direct involvement and hard work in abolishing the Licensed Prostitution System after the Liberation. It is also notable that the Licensed Prostitution Abolition Movement is a main theme in Hometown of Stars, as well as in his other works after the Liberation, such as Splendid Hell, Blue Wings and Life.

심근 허혈-재관류 백서 모델에서 mitogen-activated protein kinase 활성화에 대한 α-lipoic acid의 효과

김연경,박은미,최준호,윤경호,유남진,이은미,김남호,정진원,김민선,박병림,오석규 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

관상동맥 스텐트 삽입술을 시행한 환자에서 안지오텐신 전환효소 억제제와 안지오텐신 수용체 차단제가 재협착에 미치는 영향

김연경,정진원,윤경호,박은미,송미진,최준호,이은미,유남진,김남호,오석규 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

급성 관동맥증후군 환자의 신기능 이상과 주요 심장사건과의 관계

이상재,윤경호,최준호,송미진,김연경,박은미,김현정,이은미,유남진,김남호,오석규,정진원 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

트레드밀 운동시 흡기근 테이핑이 폐활량에 미치는 영향

김민지,신수영,송월섭,조수진,최동락,황미진,황진규,박진현,김경,Dennis W. Fell 대구대학교 특수교육재활과학연구소 2011 再活科學硏究 Vol.29 No.1

이 연구는 트레드밀 운동과 키네시오 테이핑의 효과에 따른 폐활량의 변화를 알아보기 위해 26명의 비흡연자가 참가하였으며 키네시오 테이핑을 적용하지 않은 그룹과 키네시오 테이핑을 적용한 두 그룹으로 무작위로 배정하고 각 군들을 주 3회 6주간의 트레드밀 훈련을 실시하였다. 실험 전과 후, 스파이로미터를 사용하여 키네시오 테이핑의 적용 따른 폐활량 변화 효과를 측정하였다. 이 연구의 결과를 종합해보면 키네시오 테이핑을 적용 그룹에서 적용하지 않은 그룹에 비해 FVC, FEV1에서 유의한 증가를 보였다. 이러한 결과는 건강한 성인에서 키네시오 테이핑을 적용한 경우 키네시오 테이핑을 적용하지 않고 트레드밀 운동을 한 경우보다 폐활량의 향상에 효과적이라고 생각되어진다. The purpose of this study was to investigate the variation of vital capacity(VC) according to the effects of kinesio taping with treadmill exercise. Twenty-six non-smokers were participated in this research and these subjects were randomly assigned into two groups. To measure the VC variation effects of kinesio taping, spirometer was used. The collected data were analyzed statistically by using a paired Mauchly test and repeated measure ANOVA. The results of this study were as follows; A group: treadmill with kinesio taping B group: treadmill without kinesio taping. Between A and B, there were significant differences. In the case of A group, there was 11.66% increase of VC, during 3 weeks experiment(p<.01). In the case of B group, there were significant differences, 3.35% increase of VC, during 3 weeks taping intervention(p<.01). After 6 weeks experiment, the improvement of VC shown a significant difference with intergroup (p<.05). From this result, it was revealed that treadmill exercise with kinesio taping was effective to improve VC to healthy adult than treadmill exercise without kinesio taping.

향기요법이 통증을 주소증으로 입원한 환자의 수면장애에 미치는 영향

박수곤 ( Soo Gon Park ),양미성 ( Mi Sung Yang ),김오영 ( Oh Young Kim ),조희근 ( Hee Guen Jo ),최진봉 ( Jin Bong Choi ),김선종 ( Sun Jong Kim ),설재욱 ( Jae Uk Sul ) 한방재활의학과학회 2010 한방재활의학과학회지 Vol.20 No.4

Objectives :The purpose of this study is to investigate the effects of aroma therapy on sleep disorder patients with musculoskeletal pain. Methods :The 44 patients were divided into 2 groups: Experimental group(Exp. group) was treated with aroma therapy and control group was treated except aroma therapy. To evaluate the efficiency of aroma therapy, the Korean sleep scale A(Jin-Joo Oh, Mi-Soon Song, Sin-Mi Kim. 1998) and visual analogue scale(VAS) were applied before treatment and after 5 days treatment. Results :1. The Experimental group has improved statistical significance in sleep disorder score as compare to the control group. 2. The improvement of sleep disorder score of Exp.group has difference according to impression. 3. The improvement of sleep disorder has an effect on pain decrease. Conclusions :Aroma therapy has effects of improvement on sleep disorder with musculoskeletal pain.

백서 척수후근절로부터 슈반세포의 효과적인 체외 배양법

김성민,이종호,김남열,안강민,최원재,최시호,차미주,이주영,황순정,장정원,명훈,최진영,서병무,정필훈,김명진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.4

Schwann cells(SCs), an important component of the peripheral nervous system, intract with nerous to mutually support growth and replication for the peripheral nerve regentation. Recently, ading SCs to the lumen of guidance channel is widely tried to improve regeneration or to make regeneration possible over otherwise irreparable gaps. however, it is not easy to isolate and multiplicate SCs as much as enough to help the axonal regeneration. For the allogeneic SCs source for tubular nerve guidance, we developed a little bit improved technique of harvesting and multiplicating SCs. by culturing dispersed dorsal root ganglia in specially designed medium with growth factors and serial processing, we repeatedlly generate relatively homogenous SC cultures. Our technique was compared with other methods of literature using immunostaining methods such as GFAP, S100, BDNF and the total SC count assessment at different time interval after primary culture.