방사선치료 조사영역 내에 발생한 설암 환자에서 입체조형방사선치료 경험 : 증례보고

조문준,장지영,김기환,김병국,송창준,김준상,김재성 충남대학교 암연구소 2003 암연구소 업적집 Vol.3 No.-

Objectives : We report an interim result of conformal radiotherapy in a patient with early stage cancer at the base of the tongue, which developed in a previously irradiated area. Materials and Methods : A 64-year-old male patient was diagnosed with T4NOMO supraglottic cancer. He received 72Gy of radiation therapy from 21 November 1988 to 24 February 1989. He had local failure and underwent a salvage total laryngectomy on 28 August 1989. Subsequently, he did well. In early 1999, he suffered from throat pain. He had a 2.5cm ulcerative mass at the base of his tongue, in the area that had been irradiated previously. Biopsy showed squamous cell carcinoma. After workup, he was diagnosed with base of tongue cancer with T2NOMO. Surgery was not feasible because the morbidity was not acceptable. Since it was difficult to re-irradiate the area with a curable dose using conventional 2D radiation therapy with an acceptable morbidity, we decided to try conformal radiotherapy. We used 7 static beam ports with field sizes from 7×6.4 to 8×8㎠, using 6 and 10MV photons. The fractionation regimen was 1.8Gy, 5 times per week. He received 64.8Gy in 36 fractions from 9 April 1999 to 1 June 1999. Results : In the 21 months since radiotherapy, the patient has not experienced any acute or chronic complications, such as xerostomia. He experienced relief of pain shortly after the start of radiotherapy, showed a complete response, and is still doing well. Conclusion : Conformal radiotherapy can be used to treat cancer that develops within a previously irradiated field, with curative intent.

방사선치료 조사영역 내에 발생한 설암 환자에서 입체조형방사선치료 경험 : 증례보고

조문준,장지영,김기환,김병국,송창준,김준상,김재성 충남대학교 암공동연구소 2003 암공동연구소 업적집 Vol.3 No.

Objectives : We report an interim result of conformal radiotherapy in a patient with early stage cancer at the base of the tongue, which developed in a previously irradiated area. Materials and Methods : A 64-year-old male patient was diagnosed with T4NOMO supraglottic cancer. He received 72Gy of radiation therapy from 21 November 1988 to 24 February 1989. He had local failure and underwent a salvage total laryngectomy on 28 August 1989. Subsequently, he did well. In early 1999, he suffered from throat pain. He had a 2.5cm ulcerative mass at the base of his tongue, in the area that had been irradiated previously. Biopsy showed squamous cell carcinoma. After workup, he was diagnosed with base of tongue cancer with T2NOMO. Surgery was not feasible because the morbidity was not acceptable. Since it was difficult to re-irradiate the area with a curable dose using conventional 2D radiation therapy with an acceptable morbidity, we decided to try conformal radiotherapy. We used 7 static beam ports with field sizes from 7×6.4 to 8×8㎠, using 6 and 10MV photons. The fractionation regimen was 1.8Gy, 5 times per week. He received 64.8Gy in 36 fractions from 9 April 1999 to 1 June 1999. Results : In the 21 months since radiotherapy, the patient has not experienced any acute or chronic complications, such as xerostomia. He experienced relief of pain shortly after the start of radiotherapy, showed a complete response, and is still doing well. Conclusion : Conformal radiotherapy can be used to treat cancer that develops within a previously irradiated field, with curative intent.

순비기나무 (Vitex rotundifolia L. f.) 삽수 종류 및 생장조정제 처리에 따른 발근율

Jeong Yeob Kim,Yoon Ki Hong,Song Hee Ahn,Jung Seob Moon,Eun Seok Park,Hee June Kim 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.05

Background : This study was conducted to developed the propagation method by cutting for mass cultivation of Vitex roundifolia. We were pitched the cutting two times and treated plant growth regulators to enhance the rooting percentage. Vitex roundifolia is live in beach sandy soil south of Hwanghae-Do and Gangwon-Do. Vitex roundifolia have been used to bath foam. It is good for aromatic plant. It has 0.8% essential oil content in leaf and flower. Major components of essential oil were alpha-Pinene, Sabinene, beta-Pinen, 1,8-cineole, d-Limone. Despite the superior usability, it had not yet been made by the artificial cultivation Methods and Results : We were pitched the cutting of a first-year branch on June 5, which was greenwood cutting and July 17, which was semi-hardwood cutting at Kwangseung-ri beach, Gochanggun, Jeonbuk. The length of cutting was 10cm. It had 3~4 nodes, we stuck a cutting remain 2 nodes above ground on ordinary raise seedling soil. Rooting percentage was measured at 60 days after stuck a cutting. Rooting percentage was higher greenwoody cutting(95%) than semi-hardwood cutting(57.6%). In green wood cutting, there was no significance with plant growth, but chemical injury was occurred in IBA 5,000ppm. In semi-hardwood cutting, there was significance with plant growth regulators. The rooting percentages of all the treat were higher than control(no treatment). Rooting percentage was the highest in NAA 5,000ppm treated. Conclusion : Greenwood cutting method was more proper to propagation for Vitex roundifolia than semi-hardwood cutting. The optimum time to cutting for Vitex roundifolia propagation was the early in June. If miss a time to propagation Rooting percent was elevated by plant growth regulator.

위암 환자의 복강내 투여를 위한 Activated Charcoal-Alginate Bead 제형으로부터 Mitomycin C의 용출 거동

이진호,최선웅,서중기,김동민,정경수,오정연,김진향,노승무,민병무,김용백,김창식,박근성,강대영,송규상,양준묵,조준식,정현용,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Locoregional recurrence is the most common type of recurrence in surgical operation of gastric adenocarcinoma, and peritoneal dissemination is one of the most difficult problems in advanced gastric adenocarcinoma treatment. Because the peritoneal cavity is the most common site of the first recurrence after gastric cancer resection, intraperitoneal chemotherapy seems a logical choice for cancer chemotherapy. In this study, Mitomycin C (MMC)-activated charcoal (CH)-alginate (ALG) beads were prepared by the mixtures of CH particles adsorbed with MMC as an anti-cancer drug and aqueous alginate solution. The alginate is recognized as biodegradable, nontoxic, and biocompatible. The release of MMC from the beads in 0.1 M Tris buffer was stable and continuous until about 1 week. The MMC-CH-ALG beads can be applied in the peritoneal cavity for intraperitoneal chemotherapy since they provide a good adhesiveness on the tissue and controlled release pattern of the drugs.

벼 農事의 肥料施用이 水質에 미치는 影響

김기준 건국대학교 생명과학연구원 1995 생명과학지 Vol.2 No.-

A lot of agrochemicals are applied in paddy field to increase grain yield of rice per unit area especially in the small scale farming system and it would be generated or associated with environmental problems. This experiment was carried out to evaluate the effect of fertilizer application on the quality of water in paddy field. Concentration of nitrogen, phosphorus and potassium in the surface water was increased in 2 to 3 days after fertilizer application and then decreased. Nutrients concentration in the surface water was lower in whole-layer application of fertilizer than those of surface application. But very small amount of nutrients was lost to percolating water and there was no difference between the fertilizer application methods. Nitrogen, phosphorus and potassium contents in the rice plant were higher in the whole-layer application than in the surface application of fertilizer. Removal of nitrogen, phosphorus and potassium by rice plant at 60 days after transplanting was 6, 2 and 7㎏ per 10a, respectively. This result suggested that most of applied nitrogen and potassium could be uptake by rice cropping but not for phosphorus.

Winged bean의 品種 및 施肥水準別 營養成分 및 産草量에 關한 硏究

金基駿 建國大學校附設農業資源開發硏究所 1985 農資源開發論集 Vol.10 No.-

Two winged bean cultivars, KK 1 and TPT 8, were cultivated under different fertilizer levels, and the successive variation of major nutrients contents, fresh and dry matter weights of winged bean plants were observed to investigate the possibility of utilization of this introduced new crop as a green fodder. The results obtained could be summarized as follows: 1. Varietal differences of major nutrients contents, fresh and dry matter weight were not significant. 2. Crude protein and fiber contents of winged bean plant were higher in doubled fertilizer level. Crude protein and ash contents showed their maximum value at 60-day after flowering, crude fiber contents increased up to 70-day after flowering, and content of soluble nitrogen free extracts was highest at 40-days after flowering. Interaction effect between variety and fertilizer level was found in crude protein content. 3. Higher contents of fat and nitrogen free extracts were found in standard fartilizer level compared with those in doubled fertilizer level. 4. Fresh and dry weights of winged bean plant were more in doubled fertilizer level, and showed their maximum value at 80-day after flowerning. This means that optimum cutting stage of winged bean plant for green fodder is 80-day after flowering. 5. Maximum fresh weight of winged bean plant was 7.7 tons per hectare in a variety, KK 1, and 7.9 tons per hectare in an another variety, TPT 8. As a conclusion the introduced new crop, winged bean, can be utilized as green or ensliage forage crop.

Cu/ZnO 촉매에 의한 Acrylonitrile의 水化反應

金基泰,尹奇俊,鄭泰秀 成均館大學校 科學技術硏究所 1987 論文集 Vol.38 No.1

Acrylamide was prepared by catalytic hydration of acrylonitrile. In the process of hydration of acrylonitrile, ethylene cyanohydrine was produced as a by-product. Cu/ZnO was used as the catalyst for the reaction. Cu/ZnO was prepared by reduction of Cu/ZnO with hydrogen flowing at 350℃. The reaction was carried out in the suspension of the pretreated catalyst. A simple batch reactor was used with reaction times of several hours at 70℃, 60℃, and 50℃. The results of this study are as follows: 1. The catalytic hydration of acrylonitrile is a pseudo first-order reaction. 2. The reaction products were acrylamide and ethylene cyanohydrin. The selectivity of ethylene cyanohydrin with the Cu/ZnO used in this experiment was higher than those with such catalysts as Cu, Cu/ZnO, Cu/MgO, and copper chromite which had been used by other workers. 3. The reaction rate equations are as follows: -r_AN = 9.42 x 10 exp(4)·e^[(-13,000)/RT]·C_AN(mole/l·min) r_AM = -8.68 x 10 exp(6)·e^[(-17,500/RT]·C_AN(mole/l·min) r_ECH = 4.29 x l0 exp(3)·e^[(-11,200/RT]·C_AN(mole/l·min)

Re-188이 표지된 황 교질(Sulfur Colloid)/리피오돌(Lipiodol)의 제조와 생체내 분포

김영주,정재민,김석기,손미원,이동수,정준기,이명철 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.5

목적: 리피오돌은 간암환자의 간동맥으로 투여시 암에 많이 섭취된다. 이 연구에서는 Re-188-황 교질/리피오돌을 제조하여 생체내분포를 보았다. 재료 및 방법: Re-188-황 교질/리피오돌을 마우스의 꼬리정맥으로 주사한후 1 시간 후에 생체내 분포를 확인하였다. 간암을 유발시킨 백서의 좌심실로 Re-188-황 교질/리피오돌을 주사하고 5 분후의 생체내 분포와 간의 자가방사사진을 보았다. 결과: Re-188-황 교질/리피오돌을 마우스의 꼬리정맥으로 주사한 후 1시간(n=3)섭취율(% ID/organ)은 간에서 5.2±0.7, 폐는 91.0±1.7이었다. 간암을 유발시킨 백서의 좌심실로 Re-188-황 교질/리피오돌을 주사한 후 5 분(n=4)의 정상간의 섭취율(% ID/g)은 0.41±0.28, 간암은 1.88±1.57, 폐는 1.65±1.54였으며, 자가방사사진에서도 간암부위의 섭취가 정상적인 간조직 보다 증가되었다. 결론: Re-188-황 교질/리피오돌을 동맥으로 투여시 간암부위의 섭취가 정상적인 간조직보다 많아 간암치료용 방사성의약품으로서 사용할 수 있음을 알았다. Lipiodol is used for targeting liver cancers by administrating through the hepatic artery. In the present study, feasibility of Re-188-sulfur colloid suspension in lipiodol as a liver cancer targeting agent was investigated. Materials and Methods: Re-188-sulfur colloid was prepared, harvested by centrifugation, washed with organic solvent and then suspended into lipiodol. Biodistribution of Re-188-sulfur colloid in normal saline and its suspension in lipiodol in mice after 1 hr of injection through the tail vein were investigated. Biodistribution and autoradiography of tumor-bearing liver was acquired after 5 min post-injection into left ventricle of the tumor-inoculated rats. Results: After 1 hr of injection with Re-188-sulfur colloid suspension in lipiodol through the tail vein in normal mice (n=3), the uptakes in the liver and lung were 5.2±0.7 and 91.0±1.7% ID/organ, respectively. After 5 min of injection with Re-188-sulfur colloid suspention in lipiodol through the left ventricle in the tumor-inoculated rats (n=4), uptakes in the normal liver, hepatoma, and lung were 0.41±0.28, 1.88±1.57, and 1.65±1.54% ID/organ, respectively. And autoradiography of hepatoma showed increased uptake than normal liver tissues. Conclusion: Re-188-sulfur colloid suspension in lipiodol injected through the artery shows higher uptake in the hepatoma than normal liver tissue that indicates the feasibility as a new radiopharmaceutical for therapy of hepatoma.

방사선 조사에 의한 phospholipase D 활성화 연구

김영래,김기환,조문준,임규,황병두,박승길 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1

Activation of oleate-dependent phospholipase D (PLD) isozyme by irradiation was reported previously. However, the effects of irradiation on other PLD isotypes (PLD1 and PLD2) have not been studied. There are several kinds of PLD isozymes in a cell. Therefore, it is difficult to study PLD isotype-specific activation in a cell by irradiation. We tested which isotype of PLD responds to irradiation. We used the fibroblasts cell lines overexpressing PLDl or PLD2 by introducing recombinant PLD1 or PLD2 cDNA. Phospholipids in cells were labelled with [(3)^H] myristic acid. Before irradiation, l-butanol was added. After irradiation with doses of 1, 2, and 10 Gray, we measured the formed phosphatidylbutanol containing [(3)^H] myristic acid. The overexpressed PLD isotypes were functional in those cell lines. PLD2 activity was enhanced by irradiation when we compared the PLD activations between control and PLD1 or PLD2 overexpressing cells. However PLD1 was not activated by irradiation. PLD2 isozyme, but not PLD1, was activated by irradiation. One Gray irradiation showed the maximal effect on PLD2 activation. The activation mechanism and physiological significance of PLD2 activation by irradiation remain to be uncovered.

下利의 傷寒論的 觀点과 四象醫學的 觀點의 比較·考察

金庸柱,金俊錡,崔達永 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.7 No.2

病證分析에 있어 病證을 細密히 觀察하면 體質에 따르는 특수한 病證이 있고 一般病證이라도 體質에 따르는 內的成因이 다름을 알 수 있는데, 이에 본 硏究에서는 證治醫學의 代表的인 書籍인 『傷寒論』과 體質醫學의 代表的인 書籍인 『東醫壽世保元』에서는 少陰人裏病證과 少陽人表病證에서 주로 言及되었다. 李濟馬는 三陰病證은 모두 少陰人病證이라 하였는데 下利症은 그 病機 에 있어 주로 少陰人病證과 聯關性이 많았으나, 少陰病熱化證의 경우에 있어서는 少陰人 病機로 理解하기에는 어려움이 있었으며 오히려 少陽人 病機로 推定할 수 있었다. The results of the pathogenesis analysis of a diarrhea of the differential diagnosis suggested in Sanghnnlun and Dongyishoushibaoyuan are as followings. 1. The various factors such as cold, asthenia and sthenia are the pathogenesis of a diarrhea. Among of them, the asthenia-cold is the main pathogenesis, and theis shows that the above pathogenesis could belong under the constitutional pathogensis of Shaoyinren. 2. Dong-Mu proved that all the symptoms of Sanyinbing were the symptom of Shaovinern. The case of Shanyinbing involved by the heat-evil, however, indicates that it is not the pathogenesis of Shaoyangren. 3. To understand the various differential diagnosis of the pathogenesis in Sanghanlun, the study on the theoretical relation with the constitutional pathogenesis in Dongyishibaoyuan will be necessary.