DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

Development of a Mobile Application, "Wild Flowers of Bukhansan National Park (version 1.0)", for Identification of Plants in Bukhansan National Park

Kim, Sang-Tae,Lee, Seung-Yeon,Kim, Seung-Chul,Byun, Hye-Won,Lee, Sang-Tae,Kim, Mu-Yeal,Hong, Seok-Pyo,Chung, Young-Jae,Park, Ki-Ryong,Lee, Chung-Hee,Lee, Joong-Ku,Heo, Kyeong-In,Lee, Ji-Ye,Lee, Eun-Je National Science Museum of Korea 2011 Journal of Korean nature Vol.4 No.3

We developed the educational purpose mobile application, named "Wild Flowers of Bukhansan National Park (version 1.0)", aiming for easy identification of wild flowers for students and visitors in the park. When visitors find a flower or part of plant in the park, visitors can search for its name utilizing the pictures and characters provided in their own smartphone mobile devices or tablet PCs. The application provides pictures of wild flowers in the park and character-based searching system based on 12 diagnostic features (e.g., growth form, leaf arrangement, flower symmetry, petal color, petal number, sepal number, etc). We adopted the complete floristic survey of Chung and Lee (1962) and added species that we confirmed their distribution in the park during the development of this application. In summary, number of vascular plants in this park was estimated to be 428 taxa including 100 families, 280 genera, 327 species, 1 subspecies, 50 varieties, and 5 formas. We provided a total of 588 pictures representing 358 taxa and each taxon includes multiple pictures in many cases. Included identification quizzes can be an efficient educational tool as well as fun activity for students and visitors who are learning plant species in Korea. Our next step will include GPS function in the application for indicating visitor's location and for providing previously reported sites of the species that we interested in the map of the park. The future application which includes GPS function will be a valuable tool for the monitoring of rare plants, plant researches related to the climate changes, etc. We currently provide Korean iPhone version only, and English version and both of android versions will be serviced soon.

RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

최대/최소 경계 스키마 추출 기법을 이용한 XML 문서의 DTD 추출

박경현,최은선,이종연,박정석,류근호 충북대학교 컴퓨터정보통신 연구소 2000 컴퓨터정보통신연구 Vol.8 No.2

XML이 인터넷상의 데이터 교환의 표준으로 대두되면서 데이터 모델이나 플랫폼에 관계없이 데이터의 전송이 가능하게 되었다. 특히 데이터 중심의 XML 문서의 경우 전송시의 부하를 줄이기 위해 DTD없이 전송되는 경우가 일반적이다. 그러한 이유로 전송받은 XML 데이터의 저장 효율을 높이고 질의를 최적화하며 사용자에게 편의성을 제공해주기 위해서는 XML 데이터로부터 DTD 추출이 필수적으로 요구된다. 이 논문에서는 이러한 DTD를 추출하기 위해 반구조적 데이터의 스키마 추출 기법을 적용한다. 특히, 반구조적 데이터의 최소 경계 스키마를 추출하는데 데이터로그를 이용하는 기존의 방법보다 효율적인 방법인 시뮬레이션을 적용함으로써 보다 향상된 DTD 추출 기법을 제시한다. XML makes it possible for data to be exchanged regardless of the data model in which it is represented or the platform on which it is stored, serving as a standard for data exchange format on the internet. Especially, It is natural to send XML data without DTD on the internet when XML is data-centric XML. Therefore it is needed to extract DTD to store XML data efficiently, to optimize queries, and to help users browse XML data. In this paper, Techniques for upper/lower bound schema extraction are applied to extract DTD from XML data. Especially we propose an efficient technique for extracting DTD by using simulation which is better than datalog as a lower bound schema extraction method.

스트레스와 건강행위 실천과의 연관성

박은영,박형철,박경수,손석준 大韓家庭醫學會 2000 Korean Journal of Family Medicine Vol.21 No.11

좌심실 장축 운동의 기능적 의의 (Ⅰ)

박종훈,전은석,구대회 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.2

To assess the functional significance of long axis movement which would reflect the fiber orientation of the ventricular muscle, we examined the mitral annulus motion and compared it with standard minor axis movement. M-mode echocardiogram were performed in 24 healthy individuals and 17 mitral valve replaced (MVR) patients with simultaneous EKG and phonocardiogram recordings. The results were as follows : 1. In the controls, the onset of shortening of long axis preceeds that of minor axis (phase difference : 20±3msec, mean± SEM) but in MVR patients this phase difference was reversed (-33±8msec). It means that the spherical shape changes which can be observed in normal controls during isovolumic contraction period were lost in MVR patients. 2. In MVR patients, the time intervals from A2 to the end of shortening of long axis were significantly delayed compared with normal controls (54±3 VS 90±8 msec, P<0.01). 3. In MVR patients, the delayed shortening of long axis was observed in amplitude as well as in time. In summary. we observed the regional nonuniformity between long axis and short axis in normal control and interpreted that it could play a role in modulating ventricular function which was lost in MVR patients.

한국형 후각검사 KVSS Test의 개발

홍석찬,유영석,김은서,김석천,박수홍,김진국,강성호 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.7

암수동체성 어류 Rivulus marmoratus의 치어에 미치는 몇가지 화학적 발암원의 급성 독성에 관하여

박인석,박은호 漢陽大學校環境科學硏究所 1987 環境科學論文集 Vol.8 No.-

어독성 검출계로서 암수동체성 어류 Rivulus marmoratus의 가치성을 살펴보고자 본종의 치어에 미치는 N-methyl-N'-nitro-N-nitrosoguanidine(MNNG), N-nitroso-N-methylurea(MNU)와 aflatoxin B₁(AFB₁)의 급성독성을 조사한 결과는 아래와 같다. 1. MNNG의 24hr-??값은 37.7ppm이었고 95% 신뢰한계의 상한치는 38.5ppm, 하한치는 37.0ppm이었다. MNU의 24hr-?? 값은 96.0ppm이었고 상한치는 97.9ppm, 하한치는 94.2ppm이었으며 AFB₁의 24hr-?? 값은 0.94ppm이었고 95% 신뢰한계의 상한치는 0.98ppm, 하한치는 0.90ppm이었다. 2. 모든 처리화합물은 본종에 대해 좁은 농도범위 내에서 급성 독성 효과를 나타내었으며 아울러 뚜렷한 농도반응을 보여주었다. 이상의 결과를 본종이 지닌 어독성 연구 실험동물로서의 가치성과 연관시켜 논의하였다. The acute toxicities of N-methyl-N'-nitro-N-nitrosoguanidine(MNNG), N-nitroso-N-methylurea(MNU) and aflatoxin B₁(AFB₁) to the hermaphroditic fish Rivulus marmoratus larvae were examined to evaluate the possibility of the use of this fish as the assay model of the aquatic toxicology. The results obtained were as follows; 1. The 24hr-?? to MNNG was 37.7 ppm with 95% confidence range value of 38.5~37.0ppm. The ?? for 24hours to MNU and AFB₁were found to be 96.0ppm and 0.94ppm, respectively. Their 95% confidence ranges were 97.9~94.2ppm in MNU and 0.98~0.90ppm in AFB₁. 2. The R. marmoratus larvae responded to narrow range of concentration of all tested chemicals, and toxic effects of the chemicals were clearly dose dependent. The above results were discussed in relation to the usefulness of R. marmoratus as a suitable model for aquatic toxicology.

목련과 수종과 부위에 따른 견직물의 천연염색변이

박재인,연방희,최태호,이은경,임선희,김홍은,조남석,김태동 忠北大學校 農業科學硏究所 2003 農業科學硏究 Vol.20 No.-

In order to establish a dyeing method with Magnoliaceae tree species dyeing was examined with 4 different parts, leaf, stem bark, root, flower of 4 Magnohaceae species, Magnolia kobus Dc., Magnolia denudata Desr, Magnolia obovata Thunb, Liriodendron tulipifera L Mordants used were chemicals, AIK(SO_4)_2 24H_2O, Ca(OH)_2, FeSO_4 7H_2O. All four species showed similar coloring among four different parts. Leaves and petals showed dark yellow, stem bark, grayish yellow, root bark, dark red or yellow. Al mordant made more brighter colour than Fe mordant. Stem bark showed less difference in color by mordants, whereas petals did the biggest.

의과대학생의 불안 및 자아강도가 스트레스 대처방식에 미치는 영향

박재석,류설영,장은진,김정범 대한생물치료정신의학회 2002 생물치료정신의학 Vol.8 No.1