實驗的 肝損傷 白鼠에 鹿茸 投與가 미치는 影響에 ?한 硏究

崔達永,申政圭,李尙仁,李學仁,金完熙 慶熙大學校韓醫科大學韓醫學硏究所 1979 慶熙韓醫大論文集 Vol.2 No.-

鬼箭羽(Euonymus alatus(Thunb.) Sieb)가 肝癌細胞(Hep3B)와 子宮癌細胞(HeLa)의 成長抑制에 미치는 影響에 관한 硏究

崔達永 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.7 No.2

귀전우(Euonymus alatus Sieb.)가 간암, 식도암, 위암, 자궁암등의 작종 암을 치료하는 방법으로 민방에서 많이 사용하고 있다. 실제 Euonymus alatus(Thunb.) Sieb의 추출물중 Eupolyphaga simemsis(ES)는 HDL-C/TC ratio와 LCATactivity를 증진시킨다. Human hepatoblastoma 세포인 Hep3B 세포의 성장억제를 유도하는 factor로는 TGF-β, Insulin등으로 알려져 있다. 따라서 귀전우는 간에 있어 당 대사에 관여하는 plasma HDL3-C level은 낮게 유지함으로써 간에서 지방간 이행을 억제하여 간에서의 암 발생을 억제하는 효과를 보인다. 실험에서 귀전우가 자궁암세포보다도 간암세포에 보다 높은 세포독성을 보임으로써 여러종류의 암에 모두 적용하기는 어렵다고 보여진다. 또한 귀전우가 바이러스에 의해 유도되는 간암과 hepatocellular Carcinoma 세포에서 효과가 있는지와 HCC에서 세포의 증식억제효과를 어떤 경로를 통해 이루어지는지 계속적으로 연구가 수행되어져야 될 것이다. Euonymus alatus(Thunb.) Sieb has been used for cancer theraphy such as Liver cancer, esophagus cancer, stomach cancer, and uterine cancer in folk. Eupolyphaga simemsis(ES) extracted from Euonymus alatus(Thunb.) Sieb increases HDL-C/TC ratio and LCAT activity. TGF-β and Insulin has been Knoen as factors to induce the supression of proliferation of Hep3B, Human hepatoblastoma cell. Plasma HDL3-C involved in carbohydrate methabolism in the liver was maintained in low level by Euonymus alatus(Thunb.) Sieb. The low level of plasma HDL3-C suppresses the transition from normal liver to Fat liver. Finally, this resuly in suppression of cancer in liver. In this study, Euonymus alatus(Thunb.) Sieb shows higher cell toxicity in hepatoblastoma cell(Hep3B) compared to the uterine cancer cell(HeLa). This means Euonymus alatus(Thunb.) Sieb seems not to affect in the all kinds of cancer. In future, we will study whether Euonymus alatus(Thunb.) Sieb have aneffect on the liver cancer induced by virus and hepatocellular carcinoma. Also, the mechanims of suppression of cell proliferation in HCC need to investigated.

積聚에 關한 文獻的 考察

朴明得,崔達永 대한동의병리학회 1997 동의생리병리학회지 Vol.11 No.1

惡性腫瘍이란 생체조직의 일부가 끊임없이 비정상적이고 계속적으로 과잉발육하는 것으로 1988년부터 전체 사망원인 제 1위를 차지하게 되어 국민보건문제중 중요문제로 대두하게 되었으며, 最近 韓醫學에서도 腫瘍에 대한 硏究가 活潑히 進行되고 있으나 아직 確實한 治療法이나 治療藥物에 대한 硏究는 未備한 實情이다. 따라서 著者는 腫瘍治療에 대한 韓方臨床活用에 도움이 되고자 腫瘍이 積聚의 範疇에서 取扱된다는 점에 着眠하여 積聚의 病因, 病機 및 治法에 關하여 歷代文獻을 中心으로 硏究考察하였다. 1. According to my study, because of Uh-Hyeol(瘀血; blood stasis), Dam-Yin(痰飮; phelgm-retention diseases), Yin-Sik-Bu-Jei(飮食不節; irregular diet), Chil-jeng-Son-Sang(七情損傷; seven modes of emotions), Gi-Ge-BUl-SI(起居不時; irregular life style), miss treatment of Nae-Sang-Oih-Gam-Ki-Wol(內傷外感氣鬱; disorder of internal organs, disease of cause by exogenous pathogenic factor, depressed vital energy), Oh-Jeok(五積) is produced. 2. The pathogenical mechanisms of Juk-Chui(積聚) is the accumalation of phegm, which is caused by the jam of Ki function, and blood stasis, caused by bad circulation. 3. Oh-Jeok(五積) connected with Oh-Jang(五臟; five viscera) can be divided as Gan-Jeok(肝積-肥氣; lump located at the left hypochondrium), Sini-Jeok(心積-伏粱), Bi-Jeok(脾積-비기; feeling of fullness in the chest or upper abdomen), Pyei-jeok(肺積-息實; lump located in the right hypochondrium), Sin-Jeok(賢積-奔豚; a syndrome characterized by a feeling of gas rushing up though the thorax to the thoat from lower) and they have characteristic position and symptom as follows. Gan-Jeok(肝積) is located in left hypochondrium, Sim-Jeok(心積) in upper umbilicus, Bi-Jeok(肝積) in umbillicus, Pyei-Jeok(肺積) in right hypochondrium. 4. Summarising the method of Juk-Chui(積聚) treatments, the methods are Bwo-Jeong-Beob(扶正法; supporting normal body energy) and Geo-Sa-Beob(祛邪法; eliminating the evil factors) and it is acknowledged that I-gi(理氣; the methods of regulating vital energy), Hoal-Hyeol(活血; promoting blood circulation), So-Jeok(消積; dispersing), Hoa-Eo(化瘀), Hoa-Dam(化痰; dissipating phlegm), Hae-Wol(解鬱) are used in Geo-Sa-Beob(祛邪法; eliminating the evil factors). 5. To treat Juk-Chui(積聚), I used Bwo-Jeong-Beob(扶正法; supporting normal body energy) including Dae-Bo-Ki-Hyel(大補氣血 ; invigorating premodial energy), Bo-Bi-Ik-Ki(補脾益氣; invigorating the spleen and benefit vital energy), On-Bo-Bi-Sin(溫補脾腎; warming and invigorating spleen, kidney), but the focus must be put on Bo-Ik-Bi-Ui( 補益脾胃; promoting normal energy of spleen and stomach.

柴胡疏肝散이 Stress 誘發 白鼠肝障碍의 恢復에 關한 實驗的 硏究

具立本,崔達永 대한동의병리학회 1988 동의생리병리학회지 Vol.3 No.-

This study is made to investigate the relationship of emotional exchange and Sosul function of liver . Thus, in order to observe it experimentally, I have given the heat stimulus and swimming fatigue stimulus with stressor to the rat . According to the type of stressor(heat stimulus and swimming fatigue stimulus) ,experimental mammals were divided three groups . The control group was normal rats that administrated stress, experimental group(Sample group) was normal rats that administrated extracts of SiHOSoGan - San after administration of stress . The change of glucose, ALP, total protein. bilirubin in blood serum were measured in 1st, 2nd, 4th, 6th, 8th times. Their results were as follows : 1) Variation of swimming fatigue stress group . The serum Glucose contents of control group were increased highest in 1st time and gradually decreased, the statiscal significance was recognized after 1st, 2nd time in sample group . The serum ALP activites of control group was continously increased and statiscal significance was recognized 4th, 8th time in sample group The serum bilirubin contents of contents of control group was increased to the 2nd time and gradually decreased, the statiscal significance was recognized after 2nd, 6th. 8th time in sample group. 2) Variation of heat stimulus group . The serum Glucose contents of control group were increased like swimming fatigue stress group, the statiscal significance was recognized after 4th time in sample group. The serum total protein contents of control group were increased to the 2nd time and gradually decreased, the statiscal significance was recognized 4th, 6th, 8th time in control group . By reviewing above results, it is considered that contents of serum enzyme changes according to the type of stressor, and SiHoSoGan - San is recognized to the effective for the recovery aganist the injury of liver in rat induced by stress.

失血로 誘發된 흰쥐의 血虛證에 대한 當歸補血湯의 效果

金令美,崔達永 대한동의병리학회 1988 동의생리병리학회지 Vol.3 No.-

To establish Hyol-deficiency(血虛) symptom induced by losing blood systemically, white rats were induced losing blood by cardiac puncture and used Tanggwibohyo -t'ang as a kind of experimental method . After three days. the change of RBC count, Hemoglobin value and Hematocrit value in blood were measured . The obtained results were as follows; 1. RBC count of the white rats in losing blood was reduced in control group and showed a significant change in sample group 2. Hemoglobin value of the white rats in losing blood was reduced in control group and showed a significant change in sample group 3. Hematocrit valve of the whife rats in losing blood was showed a significant change in sample group compared with control group . According to the above results, It can infer that the effects of Tanggwibohyol - tang to Hyol-deficiency (血虛) symptom induced by losing blood is significant.

寒冷과 溫熱刺戟을 받은 흰쥐의 體溫과 血淸反應에 對한 實驗的 硏究

朴元煥,崔達永 대한동의병리학회 1989 동의생리병리학회지 Vol.4 No.-

This study was planned to observed the reaction of temperature-variation occurring in a living body received cold stimulus and heat stimulus. The external condition was devised to measure Han-Yol (cold and heart) reaction. According to the type of stimulus-method(cold and heat) rats were divided two groups (C-1ㆍC-2 and H-1ㆍH-1) The change of rectal temperature and transaminase, glucose, alkaline phosphatase, total protein, albumin in blood serum were measured 0, 1, 2, 4, 6 and 8 hours after one hour exposure to cold or heat (C-1, H-1) and immediately after one cold or heat, the stimulus being 1, 2, 3, 5 and 7 hours (C-2, H-2) Their results were as follows : 1) In the rectal temperature, old-stimulus-group showed higher degree compared with heat-stimulus-group. (C-2) group showed higher significance than (C-1) group, and also. (H-1) group showed higher significance than that of (H-1) group. 2) In serm glutamic oxaloacetic transaminase activities, (C-2ㆍH-2) group showed higher significance than (C-1ㆍH-1) group, also (H-1) group showed higher significance than (C-1) group particulary, (C-2ㆍH-2) group showed an especially higher value at 3, 5 hours' stimulus group. serum glutamic pyruvic transaminase activities showed an especially higher value at 2 hour' stimulus of (C-2) group. 3) In variation of glucose level, (H-1) group showed rapidly higher value than (C-1) group, (H-2) group showed an especially higher value at 1 hour' stimulus group. 4) In alkaline phosphate activities, (C-1) group showed an especially higher value at 1 hour' stimulus group, (C-2) group showed rapidly higher significance than (H-2) group. 5) In total protein, (H-1) group (at 0, 1, 6 hours' stimulus) showed higher significance than (C-1) group, (C-2ㆍH-2) group decreased gradually as extension of stimulus hours. 6) In albumin, (C-1) group showed a statistical significance at 8 hours' stimulus, (H-2) group showed higher significance than (C-2) group. Han-Yol(cold and heat) stimulus caused metabolic abnormal stimulation and depression in the body, and the reaction of Han-Yol (cold and heat) stimulus caused relatively variation as resisting power in the body. But, in order to make an indication of Han-Yol (cold and heat) stimulus, continuous study is more necessary.

玉泉散이 ALLOXAN 投與 白鼠血淸의 代謝基質에 미치는 影響

吳政錫,崔達永 대한동의병리학회 1990 동의생리병리학회지 Vol.5 No.-

This paper is made to investigate the effect of Oak Cheun-San. In order to examine the effect of Oak Cheun-San applicable to the sogal, I induce the alloxan diabets in rats. The result of observation effecting to the metabolic substrates of serum has been found as follows. 1. In the case of the increasing of blood sugar level caused by the administration of alloxan, the blood sugar level was significantly decreased by the administration of Oak Cheun San and it was significant. 2. The amount of serum total cholesterol was increased by the administration of alloxan and it was significantly decreased in the experimental groups. It was significant. 3. The amount of serum triglyceride was significantly increased by the administration of alloxan, but it was significantly decreased in the experimental groups. 4. The activity of serum transaminase was a little increased by the administration of alloxan. The activity of the GOT was significantly decreased in the experimental groups, but the GPT was not recognized the effects of the administration of Oak Cheun-San. Therefore, as the result of the experiment Oak Cheun-San have recovered the metabolic substrates of serum which caused by alloxan diabet, so various examination in this field is essentially required further.

傷寒論 倒裝句法에 대한 文獻的 硏究

金俊錡,崔達永 대한동의병리학회 1991 동의생리병리학회지 Vol.6 No.-

신수혈의 침자극과 황기약침이 실험용 생쥐의 면역활성물질인 IL-1 α의 遺傳子發現에 미치는 影響

申相習,朴元煥,洪文燁,李泰均,崔達永 대한동의병리학회 1999 동의생리병리학회지 Vol.13 No.2

체내의 환경을 항상적으로 유지하는 생체방어체계에는 면역활성물질이 있으며, 이러한 면역활성물질의 적절한 발현이 생체의 항상성을 유지하는데 작용하게 된다. 이에 한의학에서는 생체의 항상성 유지와 체내의 면역력 증가를 위해 많은 요법들이 시행되어 왔는데, 그 중 자침요법과 약침요법이 많이 응용되고 있다. 그러나 침자극과 약침의 효과에 대한 기전은 기대하는 만큼 제시되지 못하고 있다. 이에 본 논문은 침자극과 약침의 효과에 대한 기전을 밝히는 방법의 일환으로서 면역활성물질인 IL-1α가 補腎臟, 振氣化, 祛水濕, 强腰脊, 益水壯火 등의 穴性이 있는 신수혈(BL23)의 침자극과 황기약침처치를 통해 어떻게 발현하는가를 관찰하였다. 그 결과, 면역 증강 작용을 나타내는 cytokine IL-1α 에 대한 침자극의 반응은 관찰되었고, 황기약침에 의한 IL-1α 의 발현은 명확하지 않았다. 그러므로 신수혈을 이용한 cytokine IL-1α의 추적은 황기약침의 효과보다 침자극의 효과에 대한 근거를 추측할 수 있을 것이며, 뿐만 아니라 침자극에 의한 면역반응정도를 통해 질병의 진행상태를 나타내는 진단지표로서 활용가능성이 있을 것이다. Acupuncture and Radix Astragali aqua-acupuncture stimuli have long been used to cure human diseases. However, it still remains to be unknown on its action mechanism, physiological and biochemical aspects. Thus, many attempts were made to show the scientific background covering the above mentioned mechanisms. Most recent studies show that these tests improve blood circulatory system and increase leucocyte counts. In this study, we have applied the acupuncture stimuli to mouse Sinsu(BL23), which is a stimulative point of oriental medicine, to see if cytokine such as IL-1α can be detected. Mice were treated with lipopolysaccharide(LPS) for inflammation induction, and then reverse transcriptase-polymerase chain reaction (RT-PCR) using each primer set was performed to trace the amounts of mRNA. The results are as follows ; 1. The RT-PCR product using IL-1α primers was detected as 450 bp on agarose gels and the IL-1α gene was not expressed in normal mice at 15 min after the removal of acupuncture. However, it was gradually increased indicating a faint expression after 30 min. 2. IL-1α was expressed in LPS-treated mice 30 min after the acupuncture was pulled out, and expressed most highly at 3 h after the removal of acupuncture. The gene expression of LPS-treated mice were more effective than those of normal mice. 3. IL-1α was not expressed in normal mice and LPS-treated mice from 30 min to 3hs. after Radix Astragali aqua-acupuncture stimulation. Therefore, a tracing the gene expression of cytokine IL-1α can be used more not only for a basis of the effect of acupuncture stimulation but a diagnosis guide through the immunological action than the effect of Radix Astragali aqua-acupuncture. Moreover, we need to further study the cytokine's expression in Radix Astragali aqua-acupuncture stimulation.

肝과 節의 相關性에 對한 實驗的 硏究

崔達永,申玟圭,金完熙 慶熙大學校韓醫科大學韓醫學硏究所 1983 慶熙韓醫大論文集 Vol.6 No.-