안구적출에 따른 위둔덕의 Calbindin D-28k과 c-fos의 변화에 관한 연구

김명,김기훈,김주영,하현철,안명수,김장민,조향훈,정명섭,장인엽 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Changes of Calbindin D-28k- and c-fos-immunoreactivities in the superior collicuclus after Eye Enucleation. Objective and methods: Calcium-binding proteins (CBPs) play an important role in the protection, differentiation and reorganization of the central nervous system. The effects of neonatal retinal deafferentation on a CBPs, calbindin D-28k were examined immunohistochemically in the superficial layer of the rat superior colliculus. Also early gene familly c-fos was examined to evaluate the neuronal characteristics in the superior colliculus after monocular enucleation. Results: On the experimental side of superior colliculus, the number of calbindin D-28k-immunoreactive (IR) cells was reduced (77.4% compared to control), but not fibers. Appearance of c-fos-like immunoreactivity was represented much more in the ipsilateral superior colliculus than contralateral side within 24h after eye enucleation. Conclusion: These results suggest that the changes of retinotectal projection may alter the expressional pattern of calbindin D-28k and c-fos expression.

흉부 CT 검사에서 Scan Direction이 Automatic Exposure Control을 적용한 선량에 미치는 영향에 관한 연구

남태현(Tae Hyun Nam),정용환(Yong Hwan Jeong),김주현(Joo Hyun Kim),권순안(Soon Ahn Gwon),대창민(Chang Min Dae),김성룡(Sung Ryong Kim),민관홍(Kwan Hong Min) 대한CT영상기술학회 2012 대한CT영상기술학회지 Vol.14 No.2

목적 적정한 영상의 질과 합리적인 선량위해 이용되고 있는 Automatic Exposure Control에 대한 다양한 연구가 진행되고 있는 가운데 scan direction의 영향에 대한 연구는 미비한 실정이기에, 본 논문에서는 AE를 적용한 흉부 CT검사에서 scan direction이 선량에 미치는 영향에 대해 알아보고자 하였다. 대상 및 방법 Somatom Definition Flash(Siemens Healthcare, 이하 S사), Aquilion 64(Toshiba Medical System, 이하 T사), LightSpeed VCT(General Electric Medical System, 이하 G사), Brilliance iCT(Philips healthcare, 이하 p시)와 인체 등가물질의 whole body phantom을 이용하였다. Phantom position은 supine, feet first로 위치시키고, SPR parameter 중 관전압을 120, 100, 80 kV로 각각 group 1, group 2, group 3으로 분류 후, cranio-caudal scan direction, caudo-cranial scan direction으로 각 5회 반복 scan 하고 선량 비교는 장비에서 제공하는 CTDIvol 값의 평균값을 구하여 scan direction에 따른 변화를 각 제조사별로 비교하였다. 결과 S사의 평균 CTDIvol 값을 비교해 본 결과 cranio-caudal scan direction에 대한 caudo-cranial scan direction의 증감률은 group 1에서 -4.22%, group 2에서 -5.27%, group 3에서 -4.50%로 나타났고, T사의 증감률은 group 1에서 -9.41%, group 2에서 -8.20%, group 3에서 -9.31%로 측정되었다. G사의 증감률은 group 1에서 -1.65%, group 2에서 -3.41%, group 3에서 -3.23%로 나타났으며. P사의 증감률은 group 1에서 +1.21%, group 2에서 -1.58% group 3에서 -2.41%로 나타났다. 결론 AEC를 적용한 흉부 CT검사에서 scan direction이 변화함에 따라 환자에 적용되는 선량에 차이가 발생하는 것으로 나타났다. 이러한 결과를 통해 기존에 AEC에 영향을 주는 것으로 알려진 요인 외에 scan direction 또한 영향을 미치는 것을 알 수 있었다. 따라서, 제조사별 AEC에 대한 심도있는 이해를 바탕으로 그 영향인자에 대한 추가적인 연구가 필요할 것이며, 이러한 연구들을 통해 검사부위에 맞는 최적화된 기법을 적용한다면 더욱 합리적인 선량감소를 달성할 수 있을 것으로 사료된다. I. Purpose There are many studies about automatic exposure control system. However, there is not enough study between scan direction and ABC. The purpose of this study was to compare radiation exposure dose in chest CT depending on scan direction. II. Meterial and Methods Somatom Definition Flash(Siemens Healthcare), Aquilion 64(Toshiba Medical system), LightSpeed VCT(General Electric Medical System), Brilliance iCT(Philips healthcare) and whole body phantom(KUPBU - 50 Kyoto kagaku Co.Ltd) are used in this study, Phantom position was supine, feet first. Study was devided into 3 groups depending on scan projection radiography tube voltage: group 1(120kV), group 2(100 kV), group 3(80 kV). Average CTDIvol values are compared between cranio-caudal scan direction and caudo-cranial scan direction in each group. III. Result The CTDIvol between cranio-caudal scan direction and caudo-cranial scan direction is changed. Siemens result is -4.22% in group 1, -5.27% in group 2, -4.50% in group 3. Toshiba result is -9.41% in group 1, -8.20% in group 2, -9.31% in group 3. GE result is -1.65% in group 1, -3.41% in group 2, -3.23% in group 3. Philips result is +1.21% in group 1, -1.58% in group 2, -2.41% in group 3. IV. Conclusions Radiation exposure dose was changed between cranio-caudal scan direction and caudo-cranial scan direction in Chest CT with ABC. Therefore, scan direction can affect to AEC. We should consider about many factors of AEC and create suitable protocol for each body part in order to reduce radiation dose.

Expression of cellular retinoic acid-binding protein-I (CRABP-I) in the cerebrospinal fluid of adult onset moyamoya disease and its association with clinical presentation and postoperative haemodynamic change

Jeon, Jin Sue,Ahn, Jun Hyong,Moon, Youn-joo,Cho, Won-Sang,Son, Young-Je,Kim, Seung-Ki,Wang, Kyu-Chang,Bang, Jae Seung,Kang, Hyun-Seung,Kim, Jeong Eun,Oh, Chang Wan BMJ Publishing Group Ltd 2014 Journal of neurology, neurosurgery and psychiatry Vol.85 No.7

<P><B>Objective</B></P><P>The elevation of cellular retinoic acid-binding protein-I (CRABP-I) has been suggested as a candidate in the pathogenesis of paediatric moyamoya disease (MMD). However, few studies have addressed CRABP-I in adult onset MMD. The aim of this study was to examine the expression of CRABP-I in the cerebrospinal fluid (CSF) of adult onset MMD, and to evaluate its association with clinical presentation and postoperative haemodynamic change.</P><P><B>Methods</B></P><P>This study examined the CSF from 103 patients: bilateral MMD, n=58 (56.3%); unilateral MMD, n=19 (18.4%); atherosclerotic cerebrovascular disease (ACVD), n=21 (20.4%); and control group, n=5 (4.9%). The intensity of CRABP-I was confirmed by western blotting and expressed as the median (25th–75th percentile). The differences in CRABP-I expression according to disease entity (unilateral MMD vs bilateral MMD vs ACVD), initial presenting symptoms (haemorrhage vs ischaemia) and postoperative haemodynamic change (vascular reserve in single photon emission CT and basal collateral vessels in digital subtraction angiography) were analysed.</P><P><B>Results</B></P><P>CRABP-I intensities in bilateral MMD (1.45(0.86–2.52)) were significantly higher than in unilateral MMD (0.91(0.78–1.20)) (p=0.044) or ACVD (0.85(0.66–1.11)) (p=0.004). No significant differences were noted based on the initial presenting symptoms (p=0.687). CRABP-I was not associated with improvement in vascular reserve (p=0.327), but with decrease in basal collateral vessels (p=0.023) postoperatively.</P><P><B>Conclusions</B></P><P>Higher CRABP-I in the CSF can be associated with typical bilateral MMD pathogenesis in adults. Additionally, postoperative basal collateral change may be related to the degree of CRABP-I expression.</P>

소의 대동맥 내피세포에서 산화저밀도 지단백에 의한 아포프토시스에 백일해 독소 및 콜레라 독소가 미치는 영향

손호영(Ho Young Son),강성구(Sung Ku Kang),강무일(Moo Il Kang),차봉연(Bong Youn Cha),이광우(Kwang Woo Lee),장상아(Sang Ah Chang),최윤희(Yoon Hee Choi),문성대(Sung Dae Moon),안유배(Yoo Bae Ahn),손현식(Hyun Sik Son),윤건호(Kun Ho Yoon) 대한내과학회 1999 대한내과학회지 Vol.56 No.5

N/A Objectives : Oxidized LDL is thought to play a key role in atherogenesis. Among their wide variety of biological properties, oxidized LDL injures the endothelium as an early event of atherogenesis. However, the mechanisms by which oxidized LDL injures endothelial cells are not definitely known. In order to evaluate the involvement of GTP-binding protein on the mechanism of apoptosis, we studied the effects of pertussis and cholera toxin on oxidized LDL-induced apoptosis in bovine aortic endothelial cells(BAECs). Methods : Oxidized LDL elicited apoptosis in cultured BAECs as shown by characteristic morphological and biochemical changes. Chromatin condensation and nucleus fragmentation were visualized by using fluorescence microscopy of intact cells staining by acridine orange/ ethidium bromide. DNA fragmentation was quantified by an ELISA with specific antibody for bromodeoxyuridine- labelled DNA fragments and confirmed with DNA ladder formation. Results : Studies using a combination of bacterial toxins which change the function of GTP-binding protein suggest that oxidized LDL-induced apoptosis was regulated by GTP-binding protein. Oxidized LDL-induced apoptosis was not changed by pretreatment of BAECs with pertussis toxin. In contrast, pretreatment with cholera toxin completely prevented the oxidized LDL- induced apoptosis. Conclusion : These results show that oxidized LDL induces apoptosis of BAECs and suggest that cholera toxin-sensitive G-proteins are involved in signal transduction of oxidized LDL-induced apoptosis of BAEC.

Propylthiouracil 투여에 의한 갑상선 기능저하 흰쥐 조직에서 알코올 탈수소효소 활성의 변화

백상현,김동선,이창범,박용수,안유헌,김태화,기춘석,강주섭 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.2

Background: Thyroid hormone has been known to affect hepatic alcohol dehydrogenase (ADH) activity. Although the liver is the principal site of ethanol metabolism, stomach is also responsible in part for ethanol oxidation. The effects of thyroid hormone on ADH activity in gastric mucosa and other tissues of rats had not been previously examined. Method: The effects of thyroid hormone on liver, stomach, lung, and kidney ADH activities (nM of NADH/min/mg of cytosolic protein) have been investigated in male Sprague Dawley rats treated with propylthiouracil (50 mg/kg) for 14 days. Results: Whereas hepatic ADH activities were not changed by treatment with PTU(42.9(8.6 vs 45.2 (10.1), gastric ADH activities in PTU-treated rats increased by 258.8% of control rat (6.3 ( 0.6 vs 2.2 ( 1.2, p〈0.001). In the activities of other tissues, PTU treatment decreased lung ADH activity by 59.7% of control, and increased kidney ADH activities by 247.1% of control rats. Conclusion: These data suggest that hypothyroidism causes an increase of gastric alcohol metabolism, and that the increase of gastric ADH activity can partly restore the first pass metabolism of ethanol in hypothyroid rats.

BTA드릴에 의한 심공가공시 최적절삭조건과 공구수명에 관한 연구

장성규,이충일,전언찬,안찬우,김현수 동아대학교 공과대학부설 생산기술연구소 1996 生産技術硏究所硏究論文集 Vol.1 No.2

The BTA deep hole machining has an increasing because of its wide range applications and its good productivity. The BTA tools are capable fo machining for having a large length to diameter ratio in single pass. It's really necessary that the research for machining of the deep hole drilling by the BTA drilling because its required quality should be satisfied with one time machining. In machining deep holes by single edge BTA drill, P10 class of tool material out of single tube BTA drilling system, we got the result of our study of selecting the optimum cutting condition and tool life for an object, SM55C through our testing as follows. (1) Cutting speed V=42m/min, and feed speed F=90mm/min is the optimum cutting condition and the tool life was about 10 meters. (2) The testing was shown surface roughness was 12㎛, and the roundness was less 16㎛.

정신분열병 및 정신분열형 장애에 대한 올란자핀의 효과와 안전성

안용민,강대엽,권준수,김창윤,김철응,반건호,신영민,이기철,이동우,이중서,조현상,채정호,김용식 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.4

연구목적 : 본 다기관 공동임상연구는 국내 환자를 대상으로 새로운 항정신병약물인 올라자핀의 치료 효과와 안전성을 확인하고자 시행되었다. 방 법 : 1999년 7월부터 2000년 3월까지 국내 10개 병원에 방문한 정신분열병 및 정신분열형 장애 105명을 대상으로 비대조 개방 임상연구를 시행하였다. 중등도 이상의 정신병 증상을 지니거나, 부작용으로 약물교체가 필요한 경우 또는 기분장애 증상의 치료가 필요한 환자를 대상으로 하였다. 2∼7일간의 약물 배설기간 후에 1일 10mg의 올란자핀을 투여하였고, 이후로 3∼7일 간격으로 용량을 조절하여 총 8주간 투여하였다. 치료 효과는 PANSS(Positive and Negative Syndrome Scale), BPRS(Brief Psychiatric Rating Scale), CGI(Clinical Global Impression-Severity), MADRS(Montgomery-Asberg Depression Rating Scale), HAM-A(Hamilton Rating Scale for Anxiety)로 판정하였다. 안전성 평가는 자발적인 이상반응 보고, 활력징후와 혈액 검사 및 SAS(Simpson-Angus Scale), BARS(Barnes Akathisia Rating Scale), AIMS(Abnormal Involuntary Movement Scale)척도를 이용하였다. 결 과 : 105명중에서 85.7%인 90명이 8주간의 치료를 완료하였다. 평균 최빈 용량은 일일 16.1(±4.7)mg이었고, 종료시점에서의 BPRS전체 점수가 기저 상태에 비해 40%이상 감소된 반응군은 69.5%이었다. PANSS의 양성 증후군과 일반정신병리 뿐만 아니라 음성 증후군 소척도에서도 유의한 감소를 보였으며, 이 감소들은 치료 초기부터 나타나서 8주간 지속되었다. 43.8%의 피험자가 중등도 이상의 우울증상을 같이 지니고 있었으며, 올란자핀에 의해 MADRS와 HAM-A가 유의하게 감소하였다. 치료 기간 동안에 활력징후에는 뚜렷한 변화가 없었으나 체중은 8주간 지속적으로 증가하였다. SAS와 AIMS는 감소하였으며, 정좌불능증을 제외한 나머지 추체외로 증상의 발생비율도 낮았다. 임상적인 증상이나 징후를 동반하지 않는 ALT/SGPT의 상승을 보였지만 프로락틴을 포함하는 대부분의 혈액 검사상 뚜렷한 이상 변화는 없었다. 결 론 ; 비록 본 임상연구가 비대조 개방 임상연구로서 많은 제한점을 가지고 있지만, 올란자핀이 기존 약물에 내약성을 보이거나 기분증상을 동반하는 정신병에 치료 효과가 있었다. 그리고 기존 항정신병약물 치료시에 문제가 되었던 추체외로 증상과 고프르로락틴 혈증을 포함하는 대부분의 부작용면에서 안전성을 보여 주었다. Objective : This multicenter clinical trial was carried out to investigate the efficacy and the safety of olanzpine for the treatment of Korean patients. Mothod : 105 patients with schizophrenia and schizophreniform disorder, visited at 10 mental or university hospitals, had received an open and non-comparative treatment with olanzapine for 8 weeks. Patients had psychotic or depressive symptoms with the severity above moderate degree or intolerable side effects to previous antipsychotics. After a wash-out period of 2-7 days, 10mg olanzapine was prescribed initially to all the patients, and then the dosage could be adjusted within the range of 5-20mg/day of olanzapine by 3-7 days. Results : 90(85.7%) of 105 patients completed the 8-weeks trial and the mean modal dose of olanzapine was 16.1(±4.7%)mg/day. At the end of the trial, 73 patients(69.5%) were classified as responder, which was defined as 40% or more improvement in BPRS(Brief Psychiatric Rating Scale) score comparing to baseline. There was a significant reduction in the scores of PANSS(Positive and Negative Syndrome Scale)and subscales including negative symptom scores and CGI. Also weekly analysis showed that the reductions in scores were kept on for the whole period of the trial. 43.8% of all the patients had depressive symptoms at the baseline and total scores of MADRS(Montgomery-Asberg Depression Rating Scale) and HAM-A(Hamilton Rating Scale for Anxiety) were also reduced after the trials. Vital signs revealed no clinically significant changes but continuous weight gain was observed during the treatment with olanzapine. The scores of SAS(Simpson-Angus Scale) and AIMS(Abnormal Involuntary Movement Scale) for assessing the EPS(extrapyramidal symptoms)and tardive dyskinesia respectively were significantly decreased and only a few patients reported EPS as adverse events. Although mild and clinically non-significant of ALT/SGPT was observed, most laboratory parameters including plasma prolactin level showed to significant changes during the trial. Conclusions : Although this trial had many limitations because it was a non-comparative and open study, olanzapine showed high efficacy on the positive, negative and depressive symptoms in schizophrenia and schizophreniform disorder.In addition to that, olanzapine showed a substantially favorable safety profile, such as low incidence of EPS and hyperprolactinemia.

Al 7075 合金의 時效硬化處理가 疲勞龜裂傳播에 미치는 影響

안창남,김현철,임병수 成均館大學校 科學技術硏究所 1993 論文集 Vol.44 No.2

An experimental investigation has been carried out to determine the effect of age hard-ening on the fatigue crack propagation behavior and mechanical properties using Al 7075 alloy hot extruded. Fatigue and mechanical property tests have been performed with the specimens prepared in various age hardening heat treatment conditions. Analysis of the experimental results was made to determine the relationship between Δ K_th, yield strength, % elongation and ageing temperature and time.

삼출성 중이염 환자에서의 혈중 및 아데노이드 면역글로불린 E의 면역혈청학적 연구

장혁순,강성호,유창수,김성현,이병돈,박영진,윤세동,안희영,강주원 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

Otitis media with effusion (OME) is one of the most common disease in children but its pathogenesis is not clear. In particular the role of allergy has revealed conflicting support with widely varing opinions as to its importance. Within recent years, striking biochemical and immunochemical analytic techniques have opened a new approach the study of otitis media with effusion. So we focused on distributions of IgE in adenoid tissue and serum IgE levels. Authors compared the serum IgE and the distribution of IgE in adenoid tissue between the 20 patients underwent tonsillectomy, adenoidectomy and ventilating tube insertion and control group of 11 patients underwent tonsillectomy and adenoidectomy. The results were as follows; 1. In the distribution of IgE in adenoid tissue, there was a significant increase in the group of patients with OME compared to the control group (P<0.01). 2. In the analysis of serum IgE levels, the levels of IgE in the OME patients an control group were higher than normal level, although the level of IgE in the OME patients were less than that of control group, there was no significance P>0.05).

레이저를 이용한 얇은 원통형 튜브에서의 유도초음파 발생

김현묵,정경일,장경영,안형근,곽노권,이창목 한국비파괴검사학회 2003 학술대회 논문집 Vol.- No.1

레이저를 이용하여 두께가 얇은 원통형 튜브에 유도초음파를 발생하고, 공기-결합 트랜스듀서를 이용하여 비접촉식으로 수신하였다. 원통형 튜브는 발전설비의 열교환기 및 증기발생기 튜브에 사용되는 인코넬(Inconnel)튜브이다. 레이저의 조사 경로에 선 배열 슬릿을 삽입하여 유도 초음파의 파장을 조절하였다. 파장이 일정할 때 발생되는 모드를 이론적인 분산선도로부터 예측하고, 예측된 위상속도로부터 공기-결합 트랜스듀서의 경사각을 결정하였다. 원통형 튜브에는 축대칭 모드가 지배적으로 발생하는 것으로 나타났다. 이러한 결과는 웨이블렛 변환에 의한 시간-주파수 선도와 이론적인 분산선도를 비교한 결과 증명되었다.